LOCUS       sequence-393215-        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses split-intein fused MSPdH5 for circularization during 
            expression (external His-Tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Miehling J, Goricanec D, Hagn F
  TITLE     A Split-Intein-Based Method for the Efficient Production of 
            Circularized Nanodiscs for Structural Studies of Membrane Proteins.
  JOURNAL   Chembiochem. 2018 Sep 17;19(18):1927-1933. doi: 
            10.1002/cbic.201800345. Epub 2018 Jul 30.
  PUBMED    29947468
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/cbic.201800345"; journalName: "Chembiochem"; date: 
            "2018-09-17- 17"; volume: "19"; issue: "18"; pages: "1927-1933"
COMMENT     Sequence Label: pET28a-cMSPdH5
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             31..51
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1003..1020
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcat gaaaacctgt attttcaggg cattaaaatc
       61 gcgacccgta aatatctggg caaacagaat gtctacgata ttggtgtcga acgtgaccac
      121 aactttgccc tgaaaaatgg ctttatcgca tccaactgct ttaacggtag cacctttagc
      181 aaactgcgtg aacagctggg cccggtgacc caggaatttt gggataacct ggaaaaagaa
      241 accgaaggcc tgcgtcagga aatgagcaaa gatctggaag aggtgaaagc gaaagtgcag
      301 ccgtatctgg atgactttca gaaaaaatgg caggaagaga tggaactgta tcgtcagaaa
      361 gtggaaccgc tgggcgaaga gatgcgtgat cgtgcgcgtg cgcatgtgga tgcgctgcgt
      421 acccatctgg cgccgtatag cgatgaactg cgtcagcgtc tggcggcccg tctggaagcg
      481 ctgaaagaaa acggcggtgc gcgtctggcg gaatatcatg cgaaagcgac cgaacatctg
      541 agcaccctga gcgaaaaagc gaaaccggcg ctggaagatc tgcgtcaggg cctgctgccg
      601 gtgctggaaa gctttaaagt gagctttctg agcgcgctgg aagagtatac caaaaaactg
      661 aacacccagg gttgtctgtc ctatgaaacg gaaatcctga cggtggaata cggtctgctg
      721 ccgattggta aaattgtgga aaaacgcatt gaatgcaccg tgtatagcgt tgacaacaat
      781 ggcaacattt acacgcagcc ggtcgcgcaa tggcatgacc gtggcgaaca ggaagtgttt
      841 gaatattgtc tggaagatgg ttctctgatc cgcgccacca aagatcacaa atttatgacg
      901 gtggacggtc aaatgctgcc gattgatgaa atcttcgaac gtgaactgga tctgatgcgc
      961 gttgacaacc tgccgaatga caagcttgcg gccgcactcg agcaccacca ccaccaccac
     1021 tga
//
