LOCUS       sequence-393219-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses split-intein fused MSPdH4-6 for circularization during 
            expression (internal His-Tag).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Miehling J, Goricanec D, Hagn F
  TITLE     A Split-Intein-Based Method for the Efficient Production of 
            Circularized Nanodiscs for Structural Studies of Membrane Proteins.
  JOURNAL   Chembiochem. 2018 Sep 17;19(18):1927-1933. doi: 
            10.1002/cbic.201800345. Epub 2018 Jul 30.
  PUBMED    29947468
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/cbic.201800345"; journalName: "Chembiochem"; date: 
            "2018-09-17- 17"; volume: "19"; issue: "18"; pages: "1927-1933"
COMMENT     Sequence Label: pET28a-cMSPdH4-6
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             130..147
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggcagca gcattaaaat cgcgacccgt aaatatctgg gcaaacagaa tgtctacgat
       61 attggtgtcg aacgtgacca caactttgcc ctgaaaaatg gctttatcgc atccaactgc
      121 tttaacggtc atcaccacca tcatcacagc acctttagca aactgcgtga acagctgggc
      181 ccggtgaccc aggaattttg ggataacctg gaaaaagaaa ccgaaggcct gcgtcaggaa
      241 atgagcaaag atctggaaga ggtgaaagcg aaagtgcagc cgtatagcga tgaactgcgt
      301 cagcgtctgg cggcccgtct ggaagcgctg aaagaaaacg gcggtgcgcg tctggcggaa
      361 tatcatgcga aagcgaccga acatctgagc accctgagcg aaaaagcgaa accggcgctg
      421 gaagatctgc gtcagggcct gctgccggtg ctggaaagct ttaaagtgag ctttctgagc
      481 gcgctggaag agtataccaa aaaactgaac acccagggtt gtctgtccta tgaaacggaa
      541 atcctgacgg tggaatacgg tctgctgccg attggtaaaa ttgtggaaaa acgcattgaa
      601 tgcaccgtgt atagcgttga caacaatggc aacatttaca cgcagccggt cgcgcaatgg
      661 catgaccgtg gcgaacagga agtgtttgaa tattgtctgg aagatggttc tctgatccgc
      721 gccaccaaag atcacaaatt tatgacggtg gacggtcaaa tgctgccgat tgatgaaatc
      781 ttcgaacgtg aactggatct gatgcgcgtt gacaacctgc cgaattga
//
