LOCUS       sequence-393236-        6000 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Golgi Complex, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6000)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 6000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-TGNP-N-10
FEATURES             Location/Qualifiers
     source          1..6000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      52..187
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             236..1030
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             236..1030
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(290..309)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     900..919
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1218..1237)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1262..1309
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1638..2226
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2127..2146
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2401..2704
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2705..2908
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2859..2879
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      4281..4290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4287..4289,4290..4292,4293..5006)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4332..4353)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4593..4612)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4940..4961
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5128..5249
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5165..5184)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5219..5238
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5256..5711)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5393..5414)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5605..5624
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5738..5842
                     /gene="bla"
                     /label=AmpR promoter
     promoter        join(5844..6000,1..201)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        join(5844..6000,1..35)
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5867..5887)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
       61 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
      121 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
      181 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat
      241 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
      301 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
      361 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
      421 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
      481 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
      541 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
      601 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
      661 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
      721 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
      781 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
      841 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
      901 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
      961 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     1021 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     1081 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     1141 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     1201 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     1261 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
     1321 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     1381 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     1441 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag
     1501 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1561 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1621 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1681 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1741 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1801 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1861 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1921 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1981 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2041 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2101 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2161 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2221 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2281 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
     2341 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     2401 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     2461 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     2521 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     2581 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     2641 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     2701 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     2761 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     2821 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     2881 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagctatg
     2941 ccaccatgcg gttcgtggtt gccttggtcc tcctgaacgt cgcagcggcg ggagccgtgc
     3001 cgctcttggc caccgaaagc gtcaagcaag aagaagctgg agtacggcct tctgcaggaa
     3061 acgtctccac ccaccccagc ttgagccaac ggcctggagg ctctaccaag tcgcatccgg
     3121 agccgcagac tccaaaagac agccctagca agtcgagtgc ggaggcgcag accccagaag
     3181 acacccccaa caagtcgggt gcggaggcaa agacccaaaa agacagctcc aacaagtcgg
     3241 gtgcggaggc aaagacccaa aaaggcagca ctagcaagtc gggttcggag gcgcagacca
     3301 caaaagacag cactagtaag tcgcatccgg agctgcagac tccaaaagac agcactggca
     3361 aatcgggtgc ggaggcgcag accccagaag acagccccaa caggtcgggt gcggaggcaa
     3421 agacccaaaa agacagccct agcaagtcag gttcggaggc gcagaccaca aaagatgtcc
     3481 ctaataagtc gggtgcggac ggccagaccc caaaagacgg ctccagcaag tcgggtgcgg
     3541 aggatcagac cccaaaagac gtccctaaca agtcgggtgc ggagaagcag actccaaaag
     3601 acggctctaa caagtccggt gcagaggagc agggcccaat agacgggccc agcaagtcgg
     3661 gtgcggagga gcagacctca aaagacagcc ctaacaaggt ggttccagag cagccttcct
     3721 ggaaagacca ttccaagccc atctccaacc cttctgataa caaggagctc cccaaggctg
     3781 acacaaacca gcttgctgac aaagggaagc tttctcctca tgctttcaaa accgaatctg
     3841 gggaggaaac tgacctcatt tctcccccgc aggaggaagt taagtcttca gagcctactg
     3901 aggatgtgga gcccaaagag gctgaagatg atgatacagg acccgaggag ggctcaccgc
     3961 ccaaagaaga gaaagaaaag atgtccggtt ctgcctccag tgagaaccgt gaaggaacac
     4021 tttcggattc cacgggtagc gagaaggatg acctttatcc gaacggttct ggaaatggca
     4081 gcgcggagag cagccacttc tttgcatatc tggtgactgc agccattctt gtggctgtcc
     4141 tctatatcgc tcatcacaac aagcggaaga tcattgcttt tgtcctggaa ggaaaaagat
     4201 ctaaagtcac ccggcggcca aaggccagtg actaccaacg tttggaccag aagtccagct
     4261 ccggagggga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     4321 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     4381 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     4441 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt gacctacggc gtgcagtgct
     4501 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     4561 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     4621 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     4681 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaaggtct
     4741 atatcaccgc cgacaagcag aagaacggca tcaaggtgaa cttcaagacc cgccacaaca
     4801 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     4861 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc
     4921 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     4981 tcggcatgga cgagctgtac aagtaagcgg ccgcgactct agatcataat cagccatacc
     5041 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     5101 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     5161 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     5221 ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt
     5281 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     5341 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     5401 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     5461 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     5521 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     5581 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     5641 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     5701 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     5761 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     5821 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
     5881 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5941 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
//
