LOCUS       sequence-393239-        5861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5861)
  AUTHORS   Kanchanawong P, Shtengel G, Pasapera AM, Ramko EB, Davidson MW, Hess
            HF, Waterman CM
  TITLE     Nanoscale architecture of integrin-based cell adhesions.
  JOURNAL   Nature. 2010 Nov 25;468(7323):580-4. doi: 10.1038/nature09621.
  PUBMED    21107430
REFERENCE   2  (bases 1 to 5861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-11-25- 25"; volume: "468"; issue: "7323"; 
            pages: "580-4"
COMMENT     Sequence Label: mEos2-VASP-5
FEATURES             Location/Qualifiers
     source          1..5861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             73..867
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             73..867
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(127..146)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     737..756
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1055..1074)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1099..1146
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1475..2063
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1964..1983
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2238..2541
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2542..2745
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2696..2716
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2790..3467
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    4826..4947
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4863..4882)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4917..4936
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4954..5409)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5091..5112)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5303..5322
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5436..5540
                     /gene="bla"
                     /label=AmpR promoter
     promoter        join(5542..5861,1..38)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5542..5733
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5565..5585)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      join(5750..5861,1..24)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga
       61 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
      121 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
      181 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
      241 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
      301 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
      361 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
      421 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
      481 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
      541 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
      601 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
      661 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
      721 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
      781 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
      841 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
      901 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
      961 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     1021 tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg
     1081 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt
     1141 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
     1201 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
     1261 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt
     1321 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     1381 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1441 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     1501 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     1561 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     1621 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1681 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1741 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1801 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     1861 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     1921 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     1981 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     2041 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     2101 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     2161 gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt agttcatagc
     2221 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     2281 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2341 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2401 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2461 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2521 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2581 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2641 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2701 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     2761 cagatccgct agcgctaccg gtcgccacca tgagtgcgat taagccagac atgaagatca
     2821 aactccgtat ggaaggcaac gtaaacgggc accactttgt gatcgacgga gatggtacag
     2881 gcaagccttt tgagggaaaa cagagtatgg atcttgaagt caaagagggc ggacctctgc
     2941 cttttgcctt tgatatcctg accactgcat tccattacgg caacagggta ttcgccaaat
     3001 atccagacaa catacaagac tattttaagc agtcgtttcc taaggggtat tcgtgggaac
     3061 gaagcttgac tttcgaagac gggggcattt gcattgccag aaacgacata acaatggaag
     3121 gggacacttt ctataataaa gttcgatttt atggtaccaa ctttcccgcc aatggtccag
     3181 ttatgcagaa gaagacgctg aaatgggagc cctccactga gaaaatgtat gtgcgtgatg
     3241 gagtgctgac gggtgatatt catatggctt tgttgcttga aggaaatgcc cattaccgat
     3301 gtgacttcag aactacttac aaagctaagg agaagggtgt caagttacca ggctaccact
     3361 ttgtggacca ctgcattgag attttaagcc atgacaaaga ttacaacaag gttaagctgt
     3421 atgagcatgc tgttgctcat tctggattgc ctgacaatgc cagacgatcc ggactcagat
     3481 ccatgagcga gacggtcatc tgttccagcc gggctactgt gatgctttat gatgacagca
     3541 acaagcgatg gctccctgct ggcactggtc cccaggcctt cagccgcgtc cagatctacc
     3601 acaaccccac tgctaactcc ttccgagttg ttggccgcaa gatgcagccg gatcagcagg
     3661 tggttatcaa ctgtgccatc attcggggtg tcaagtacaa tcaggccact cccatcttcc
     3721 atcagtggcg agatgcccgc caggtctggg gcctcaactt cggcagcaag gaggacgcca
     3781 tacagtttgc aacaggcatg gccaacgccc tagaggcctt ggaaggaggt gggcctcccc
     3841 cagccccagc accccctgcc tggtctgccc agaatggtcc ctccccagag gagctggaac
     3901 aacagaaaag gcagccggag catatggagc gccgggtctc caatgcagga ggcccacctg
     3961 ctcccccagc tgggggccct cctccacctc caggacctcc ccctcctcca ggtccccccc
     4021 cacccccagg tctgccctcc tcaggggtat ctggggcagg tcatggagca ggggcagccc
     4081 caccccctgc acccccactc cctacaacac agggccccaa tagtgggggt tccggggccc
     4141 caggcctggc tgctgccatt gctggagcca aactcaggaa agtgagcaag caggaggagg
     4201 cctctggggg gcccctggcc cccaaagctg agaacagtcg aagcactggt ggggggctta
     4261 tggaagagat gaacgccatg ctggcccgga gaagaaaagc cacacaggtt ggggagaagc
     4321 cccccaaaga cgagtcagcc agtgaggagt cagaggcccg actccctgcc cagagtgaac
     4381 ctgtgagaag accctgggag aagaacagca caaccttgcc aaggatgaag tcgtcctctt
     4441 ctgtgactac ctccgaggcc cacccctcga cgccctgctc cagtgatgac tccgacttgg
     4501 agagggtgaa gcaggagctt ctggaagagg tgcggaagga gctacagaaa atgaaagagg
     4561 aaatcatcga agtctttgtc caggagctga ggaagcgggg ttctccttga gcggccgcca
     4621 ccgcggtgga gctcggtacc ccgggtcgac ctgcagccaa gcttcgaatt ctgcagtcga
     4681 cggtaccgcg ggcccgggat ccaccggatc tagataactg atcataatca gccataccac
     4741 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     4801 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     4861 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     4921 tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat attttgttaa
     4981 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     5041 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     5101 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     5161 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     5221 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     5281 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     5341 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     5401 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5461 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5521 aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     5581 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     5641 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     5701 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     5761 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     5821 agaggccgag gccgcctcgg cctctgagct attccagaag t
//
