LOCUS       sequence-393243-        4780 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 400 / 504, Emission: 515 / 517.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4780)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPA-GFP-Farnesyl-5
FEATURES             Location/Qualifiers
     source          1..4780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             22..816
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             22..816
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(76..95)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     686..705
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1004..1023)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1048..1095
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1424..2012
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1913..1932
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2187..2490
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2491..2694
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2645..2665
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2733..2742
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2739..2741,2742..2744,2745..3455)
                     /codon_start=1
                     /product="photoactivatable GFP variant (Patterson et al., 
                     2002)"
                     /label=PA-GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2784..2805)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3045..3064)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3392..3413
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3694..3815
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3731..3750)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3785..3804
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3822..4277)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3959..3980)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4171..4190
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4304..4408
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4410..4767
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4410..4601
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4433..4453)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4618..4753
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
       61 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
      121 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
      181 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg
      241 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
      301 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
      361 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
      421 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
      481 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
      541 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
      601 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
      661 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
      721 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
      781 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
      841 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
      901 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
      961 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga
     1021 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg
     1081 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc
     1141 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc
     1201 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag
     1261 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     1321 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     1381 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     1441 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     1501 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     1561 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     1621 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     1681 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     1741 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     1801 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     1861 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     1921 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     1981 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     2041 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     2101 tggataaccg tattaccgcc atgcattagt tattaatagt aatcaattac ggggtcatta
     2161 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     2221 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     2281 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     2341 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     2401 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     2461 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2521 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2581 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2641 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta
     2701 gtgaaccgtc agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagc
     2761 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     2821 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     2881 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcagctacg
     2941 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     3001 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     3061 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     3121 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     3181 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggcc aacttcaaga
     3241 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     3301 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagccac cagtccaagc
     3361 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     3421 ccgggatcac tctcggcatg gacgagctgt acaagtccgg actcagatct aagctgaacc
     3481 ctcctgatga gagtggcccc ggctgcatga gctgcaagtg tgtgctctcc tgagaattct
     3541 gcagtcgacg gtaccgcggg cccgggatcc accggatcta gataactgat cataatcagc
     3601 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     3661 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     3721 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     3781 agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat
     3841 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     3901 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     3961 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     4021 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     4081 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     4141 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     4201 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     4261 gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     4321 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     4381 gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
     4441 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4501 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4561 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4621 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4681 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     4741 ttttttggag gcctaggctt ttgcaaagat cgatcaagag
//
