LOCUS       sequence-393263-        6170 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing P2 tail fiber H and chaperone protein G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Fa-Arun J, Huan YW, Darmon E, Wang B
  TITLE     Tail-Engineered Phage P2 Enables Delivery of Antimicrobials into 
            Multiple Gut Pathogens.
  JOURNAL   ACS Synth Biol. 2023 Feb 17;12(2):596-607. doi: 
            10.1021/acssynbio.2c00615. Epub 2023 Feb 2.
  PUBMED    36731126
REFERENCE   2  (bases 1 to 6170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00615"; journalName: "ACS Synth Biol"; date: 
            "2023-02-17- 17"; volume: "12"; issue: "2"; pages: "596-607"
COMMENT     Sequence Label: pP2_tail_fiber
FEATURES             Location/Qualifiers
     source          1..6170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    26..47
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             2718..2735
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2788..2806)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2802..2849
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    2881..2901
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      2902..2959
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     CDS             complement(3262..4212)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      4260..4482
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(4974..5068)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(5098..5889,5890..5958))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIQYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5721..5740
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5959..6063)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6128..6146
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(join(6150..6170,1..23))
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagagtac gctctccttg
       61 aatgttgtct ggtagttcta caaatgaatc cagatagcat aacttttata tattgtgcaa
      121 tctcacatgc atgagcataa aattcagaac cgttatcacc actgccggtg cagcaaagct
      181 ggcagcggca accgcgccgg gagggcggaa ggtcaacatt accacgatgg ccgtcgggga
      241 tggcggtggt aaattgcctg tcccggatgc cggacagacc gggcttatcc atgaagtctg
      301 gcgacatgcg ctgaacaaaa tcagccagga caaacgaaac agtaattata ttatcgccga
      361 gctggttatt ccgccggagg tgggcggttt ctggatgcgt gagcttggcc tgtacgatga
      421 tgcgggaacg ttaattgccg tggcgaacat ggccgaaagc tataagccag cccttgccga
      481 aggctcagga cgttcgcaga cctgtcgcat ggtcatcatc gtcagcagtg tggcctcagt
      541 ggagctgacc attgacacca caacggtgat ggcgacgcag gattacgttg atgacaaaat
      601 tgcagagcac gaacagtcac gacgtcaccc ggacgcctcg ctgacagcaa aaggttttac
      661 tcagttaagc agtgcgacca acagcacgtc tgaaacactg gccgcaacgc cgaaagcggt
      721 aaaggccgcg tatgacctgg ctaacgggaa atataccgca caggacgcca ccacagcgcg
      781 aaaaggcctt gtccagctta gtagcgccac caacagcacg tctgaaacgc tcgccgcaac
      841 accaaaagcc gttaagacgg taatggatga aacgaacaaa aaagcgccat taaacagccc
      901 tgcactgacc ggaacgccaa cgacgccaac tgcgcgacag ggaacgaata atactcagat
      961 cgcaaacacg gctttcgtta tggccgcgat tgccgccctt gtagactcgt cgcctgacgc
     1021 actgaatacg ctgaacgagc tggcggcggc gctgggcaat gacccgaatt ttgctaccac
     1081 catgactaat gcgcttgcgg gtaagcaacc gaaagatgct accctgacgg cgctggcggg
     1141 gcttgctact gcggcagaca ggtttccgta ttttacgggg aatgatgttg ccagcctggc
     1201 gaccctgaca aaagtcgggc gggatattct ggctaaatcg accgttgccg ccgttatcga
     1261 atatctcggt ttacaggaaa cggtaaaccg agccgggaac gccgtgcaaa aaaatggcga
     1321 taccttgtcc ggtggactta cttttgaaaa cgactcaatc cttgcctgga ttcgaaatac
     1381 tgactgggcg aagattggat ttaaaaatga tgccgatggt gacactgatt catacatgtg
     1441 gtttgaaacg ggggataacg gcaatgaata tttcaaatgg agaagccgcc agagtaccac
     1501 aacaaaagac ctgatgacgt tgaaatggga tgcactaaat attcttgtta atgccgtcat
     1561 taatggctgt tttggagttg gtacgacgaa tgcactaggt ggtagctcta ttgttcttgg
     1621 tgataatgat accggattta aacagaatgg agacggtatt cttgatgttt atgctaacag
     1681 tcagcgtgta ttccgttttc agaatggagt ggctattgct tttaaaaata ttcaggcagg
     1741 tgatagtaaa aagttctcgc tatccagctc taatacatcc acgaagaata ttacctttaa
     1801 tttatggggt gcttccaccc gtccagtggt tgcagagtta ggcgatgagg ccggatggca
     1861 tttctatagc cagcgaaata cagataactc ggtaatattt gctgttaacg gtcagatgca
     1921 acccagcaac tggggaaatt ttgattcccg ctatgtgaaa gatgttcgcc tgggtacgcg
     1981 agttgttcaa ttgatggcgc gaggtggtcg ttatgaaaaa gccggacaca cgattaccgg
     2041 attaagaatc attggtgaag tagatggcga tgatgaagcc atcttcaggc cgatacaaaa
     2101 atacatcaat ggcacatggt ataacgttgc gcaggtgtaa gttatgcagc atttaaagaa
     2161 cattaagtca ggtaatccaa aaacaaaaga gcaatatcag ctaacaaaga attttgatgt
     2221 tatctggtta tggtccgaag acggaaaaaa ctggtatgag gaagtgaaga actttcagcc
     2281 agacacaata aagattgttt acgatgaaaa taatattatt gtcgctatca ccagagatgc
     2341 ttcaacgctt aatcctgaag gttttagcgt tgttgaggtt cctgatatta cctccaaccg
     2401 acgtgctgac gactcaggta aatggatgtt taaggatggt gctgtggtta aacggattta
     2461 tacggcagat gaacagcaac aacaggcaga atcacaaaag gccgcgttac tttccgaagc
     2521 ggaaaacgtt attcagccac tggaacgcgc tgtcaggctg aatatggcga cggatgagga
     2581 acgtgcacga ctggagtcat gggaacgtta cagcgttctg gtcagccgtg tggatcctgc
     2641 aaatcctgaa tggccggaaa tgccgcaata aggatccgaa ttcgagctcc gtcgacaagc
     2701 ttgcggccgc actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc
     2761 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
     2821 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattaataa
     2881 tactagtagc ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt
     2941 ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcact
     3001 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatctcgag
     3061 gttacattgt cgatctgttc atggtgaaca gctttgaatg caccaaaaac tcgtaaaagc
     3121 tctgatgtat ctatcttttt tacaccgttt tcatctgtgc atatggacag ttttcccttt
     3181 gatatgtaac ggtgaacagt tgttctactt ttgtttgtta gtcttgatgc ttcactgata
     3241 gatacaagag ccataagaac ctcagatcct tccgtattta gccagtatgt tctctagtgt
     3301 ggttcgttgt ttttgcgtga gccatgagaa cgaaccattg agatcatact tactttgcat
     3361 gtcactcaaa aattttgcct caaaactggt gagctgaatt tttgcagtta aagcatcgtg
     3421 tagtgttttt cttagtccgt tatgtaggta ggaatctgat gtaatggttg ttggtatttt
     3481 gtcaccattc atttttatct ggttgttctc aagttcggtt acgagatcca tttgtctatc
     3541 tagttcaact tggaaaatca acgtatcagt cgggcggcct cgcttatcaa ccaccaattt
     3601 catattgctg taagtgttta aatctttact tattggtttc aaaacccatt ggttaagcct
     3661 tttaaactca tggtagttat tttcaagcat taacatgaac ttaaattcat caaggctaat
     3721 ctctatattt gccttgtgag ttttcttttg tgttagttct tttaataacc actcataaat
     3781 cctcatagag tatttgtttt caaaagactt aacatgttcc agattatatt ttatgaattt
     3841 ttttaactgg aaaagataag gcaatatctc ttcactaaaa actaattcta atttttcgct
     3901 tgagaacttg gcatagtttg tccactggaa aatctcaaag cctttaacca aaggattcct
     3961 gatttccaca gttctcgtca tcagctctct ggttgcttta gctaatacac cataagcatt
     4021 ttccctactg atgttcatca tctgagcgta ttggttataa gtgaacgata ccgtccgttc
     4081 tttccttgta gggttttcaa tcgtggggtt gagtagtgcc acacagcata aaattagctt
     4141 ggtttcatgc tccgttaagt catagcgact aatcgctagt tcatttgctt tgaaaacaac
     4201 taattcagac atacatctca attggtctag gtgattttaa tcactatacc aattgagatg
     4261 ggctagtcaa tgataattac atgtcctttt cctttgagtt gtgggtatct gtaaattctg
     4321 ctagaccttt gctggaaaac ttgtaaattc tgctagaccc tctgtaaatt ccgctagacc
     4381 tttgtgtgtt ttttttgttt atattcaagt ggttataatt tatagaataa agaaagaata
     4441 aaaaaagata aaaagaatag atcccagccc tgtgtataac tcactacttt agtcagttcc
     4501 gcagtattac aaaaggatgt cgcaaacgct gtttgctcct ctacaaaaca gaccttaaaa
     4561 ccctaaaggc ttaagtagca ccctcgcaag ctcgggcaaa tcgctgaata ttccttttgt
     4621 ctccgaccat caggcacctg agtcgctgtc tttttcgtga cattcagttc gctgcgctca
     4681 cggctctggc agtgaatggg ggtaaatggc actacaggcg ccttttatgg attcatgcaa
     4741 ggaaactacc cataatacaa gaaaagcccg tcacgggctt ctcagggcgt tttatggcgg
     4801 gtctgctatg tggtgctatc tgactttttg ctgttcagca gttcctgccc tctgattttc
     4861 cagtctgacc acttcggatt atcccgtgac aggtcattca gactggctaa tgcacccagt
     4921 aaggcagcgg tatcatcaac aggcttaccc gtcttactgt ccctagtgct tggattctca
     4981 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
     5041 gtcattactg gatctatcaa caggagtcca agcgagctcg taaacttggt ctgacagtta
     5101 ccaatgctta atcagtgagg cacctatctc agctatctgt ctatttcgtt catccatagt
     5161 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     5221 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     5281 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     5341 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     5401 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     5461 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     5521 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     5581 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     5641 gactggtgag tactcaacca agtcattctg agaatattgt atgcggcgac cgagttgctc
     5701 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     5761 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     5821 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     5881 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     5941 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     6001 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     6061 gcgcacattt cccatggtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     6121 ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct
//
