LOCUS       sequence-393266-        6080 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing chimeric P2-phiV10 tail fiber.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Fa-Arun J, Huan YW, Darmon E, Wang B
  TITLE     Tail-Engineered Phage P2 Enables Delivery of Antimicrobials into 
            Multiple Gut Pathogens.
  JOURNAL   ACS Synth Biol. 2023 Feb 17;12(2):596-607. doi: 
            10.1021/acssynbio.2c00615. Epub 2023 Feb 2.
  PUBMED    36731126
REFERENCE   2  (bases 1 to 6080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00615"; journalName: "ACS Synth Biol"; date: 
            "2023-02-17- 17"; volume: "12"; issue: "2"; pages: "596-607"
COMMENT     Sequence Label: pP2-phiV10
FEATURES             Location/Qualifiers
     source          1..6080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    26..47
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             2628..2645
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2698..2716)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2712..2759
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    2791..2811
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      2812..2869
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     CDS             complement(3172..4122)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      4170..4392
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(4884..4978)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(5008..5799,5800..5868))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIQYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5631..5650
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5869..5973)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6038..6056
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(join(6060..6080,1..23))
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagagtac gctctccttg
       61 aatgttgtct ggtagttcta caaatgaatc cagatagcat aacttttata tattgtgcaa
      121 tctcacatgc atgagcataa aattcagaac cgttatcacc actgccggtg cagcaaagct
      181 ggcagcggca accgcgccgg gagggcggaa ggtcaacatt accacgatgg ccgtcgggga
      241 tggcggtggt aaattgcctg tcccggatgc cggacagacc gggcttatcc atgaagtctg
      301 gcgacatgcg ctgaacaaaa tcagccagga caaacgaaac agtaattata ttatcgccga
      361 gctggttatt ccgccggagg tgggcggttt ctggatgcgt gagcttggcc tgtacgatga
      421 tgcgggaacg ttaattgccg tggcgaacat ggccgaaagc tataagccag cccttgccga
      481 aggctcagga cgttcgcaga cctgtcgcat ggtcatcatc gtcagcagtg tggcctcagt
      541 ggagctgacc attgacacca caacggtgat ggcgacgcag gattacgttg atgacaaaat
      601 tttagcggca aatgatggat ttaagtttat tggtcagtgc cctgatatat tgacactaag
      661 aacaatagaa cctgaaaaaa atggtcaaag gatcacgttg cgacagcata cgatcggaac
      721 tgggcttggt ggaggggtat tcagagctgt acttgatgga actggctata ccgatgatga
      781 tggtgttgta ataaaaacag caggtggttc tgtatggtta cgggttaatg cagataaggt
      841 taatccgttt atgtttgggg ccactggcgt ggctgatgat acagcagcac tgcaaaagat
      901 gcttgaatgt ggcagggcgg ctgagcttgg cacaaatgtc tggaaagcat ctaatcttga
      961 gctaaataac aagtcgtgtt cactatctgg gtctggtcta cacgtttcca ggatagagca
     1021 aatatctggg gcaacaggcg cgttactgac aattactcag gattgcagcc tgatttattt
     1081 gtccgattgt ggcctgtatg gggatggaat aactgcggga acaagcgggg taacgatgga
     1141 gacaggaaac ccaggtgggg caccaagcta tccattcaat accgcccctg acgttcgtag
     1201 ggatctttac attagcaatg ttcacattac aggatttgac gagttaggat ttgattatcc
     1261 tgaaacgaat ttttccgtct caacgcatgg attattcata agaaacatta aaaaaaccgg
     1321 ggctaagatc ggaactaccg attttacatg gacaaacctt caaattgata cgtgtgggca
     1381 ggaatgctta gtgcttgatg gtgctggtaa ttgcagaatt ataggtgcca aattaatttg
     1441 ggctggttca gaaaatgaaa caccatattc aggattacgt atttctaatt cacaaaatgt
     1501 aaacatgaca ggagttgagt tacaggactg tgcatacgat ggattgtata ttaaaaattc
     1561 aactgttgct atcagtgggt tgaatactaa cagaaatagt gcatcatcaa atctgtcata
     1621 ccataatatg gtttttgaaa acagtatagt aactgtagat ggttatgttt gccgtaacta
     1681 tgctgctaca tcgctatatg atctaaattc acaagccgga aatgtgaggt gtattggttc
     1741 cgatagtacc gtattgataa atggtattta cgagtcagaa gtaaacagcg aacgactcat
     1801 gggtgacaat aatcttattc agccatattc tggtgattta ataataaatg gcctaaaaaa
     1861 ctattataca tatacaggaa gcgtaaaaaa caatattcct acatttgacg gagtcgttac
     1921 aaccgcaaca tatgtaagcg ctccttcgat actagggcaa ggaaacatgc ttaagttaac
     1981 gcaatcaaac aaggataagt tattattttc tgataaagta tcgagacatg ggtgtacaat
     2041 cggccttgtt ctaatcccgt catttacggg tgcaacaact atgacagctt tcactctggg
     2101 gagtggatat tcgccctctg gaaactctgc tgttatgcaa tttatagtta actccagtgg
     2161 tgtgcagact atagcaatat tattatccgg tgatggtata actcaaacgt taacaagcga
     2221 tctgacaacg gagcaagcgt tagcttcagg aggcgtatat cattttgcga tgggattcgc
     2281 tccaggccga ttatggtggt ctattattga tataaatacc ggaaggcgta tacgacgggc
     2341 gtacagacaa ccagatcttc acgccgcatt taatagcata ttcaacagcg gaacatcttc
     2401 tatcaccgca ttttcaggac cattagcagg tgatattgca tgtgaagggg caggttcgca
     2461 tgtttatgtt ggcggtttct catctgagtc agactacgca gcgagcagaa tgtacggatt
     2521 atttacccca gtagacctgg ataaacaata ctcattccgc acgctgaacg gaaatattta
     2581 aggatccgaa ttcgagctcc gtcgacaagc ttgcggccgc actcgagcac caccaccacc
     2641 accactgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
     2701 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
     2761 tgaaaggagg aactatatcc ggattaataa tactagtagc ggccgctgca gtccggcaaa
     2821 aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa ccgaaaagat tacttcgcgt
     2881 tatgcaggct tcctcgctca ctgactcact gcgctcggtc gttcggctgc ggcgagcggt
     2941 atcagctcac tcaaaggcgg taatctcgag gttacattgt cgatctgttc atggtgaaca
     3001 gctttgaatg caccaaaaac tcgtaaaagc tctgatgtat ctatcttttt tacaccgttt
     3061 tcatctgtgc atatggacag ttttcccttt gatatgtaac ggtgaacagt tgttctactt
     3121 ttgtttgtta gtcttgatgc ttcactgata gatacaagag ccataagaac ctcagatcct
     3181 tccgtattta gccagtatgt tctctagtgt ggttcgttgt ttttgcgtga gccatgagaa
     3241 cgaaccattg agatcatact tactttgcat gtcactcaaa aattttgcct caaaactggt
     3301 gagctgaatt tttgcagtta aagcatcgtg tagtgttttt cttagtccgt tatgtaggta
     3361 ggaatctgat gtaatggttg ttggtatttt gtcaccattc atttttatct ggttgttctc
     3421 aagttcggtt acgagatcca tttgtctatc tagttcaact tggaaaatca acgtatcagt
     3481 cgggcggcct cgcttatcaa ccaccaattt catattgctg taagtgttta aatctttact
     3541 tattggtttc aaaacccatt ggttaagcct tttaaactca tggtagttat tttcaagcat
     3601 taacatgaac ttaaattcat caaggctaat ctctatattt gccttgtgag ttttcttttg
     3661 tgttagttct tttaataacc actcataaat cctcatagag tatttgtttt caaaagactt
     3721 aacatgttcc agattatatt ttatgaattt ttttaactgg aaaagataag gcaatatctc
     3781 ttcactaaaa actaattcta atttttcgct tgagaacttg gcatagtttg tccactggaa
     3841 aatctcaaag cctttaacca aaggattcct gatttccaca gttctcgtca tcagctctct
     3901 ggttgcttta gctaatacac cataagcatt ttccctactg atgttcatca tctgagcgta
     3961 ttggttataa gtgaacgata ccgtccgttc tttccttgta gggttttcaa tcgtggggtt
     4021 gagtagtgcc acacagcata aaattagctt ggtttcatgc tccgttaagt catagcgact
     4081 aatcgctagt tcatttgctt tgaaaacaac taattcagac atacatctca attggtctag
     4141 gtgattttaa tcactatacc aattgagatg ggctagtcaa tgataattac atgtcctttt
     4201 cctttgagtt gtgggtatct gtaaattctg ctagaccttt gctggaaaac ttgtaaattc
     4261 tgctagaccc tctgtaaatt ccgctagacc tttgtgtgtt ttttttgttt atattcaagt
     4321 ggttataatt tatagaataa agaaagaata aaaaaagata aaaagaatag atcccagccc
     4381 tgtgtataac tcactacttt agtcagttcc gcagtattac aaaaggatgt cgcaaacgct
     4441 gtttgctcct ctacaaaaca gaccttaaaa ccctaaaggc ttaagtagca ccctcgcaag
     4501 ctcgggcaaa tcgctgaata ttccttttgt ctccgaccat caggcacctg agtcgctgtc
     4561 tttttcgtga cattcagttc gctgcgctca cggctctggc agtgaatggg ggtaaatggc
     4621 actacaggcg ccttttatgg attcatgcaa ggaaactacc cataatacaa gaaaagcccg
     4681 tcacgggctt ctcagggcgt tttatggcgg gtctgctatg tggtgctatc tgactttttg
     4741 ctgttcagca gttcctgccc tctgattttc cagtctgacc acttcggatt atcccgtgac
     4801 aggtcattca gactggctaa tgcacccagt aaggcagcgg tatcatcaac aggcttaccc
     4861 gtcttactgt ccctagtgct tggattctca ccaataaaaa acgcccggcg gcaaccgagc
     4921 gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca
     4981 agcgagctcg taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     5041 agctatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     5101 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     5161 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     5221 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     5281 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     5341 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     5401 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     5461 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     5521 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     5581 agaatattgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     5641 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     5701 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     5761 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     5821 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     5881 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     5941 tatttagaaa aataaacaaa taggggttcc gcgcacattt cccatggtgc cacctgacgt
     6001 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa
     6061 tttcagataa aaaaaatcct
//
