LOCUS       sequence-393267-        6869 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid expressing chimeric P2-P1(S') tail fiber.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Fa-Arun J, Huan YW, Darmon E, Wang B
  TITLE     Tail-Engineered Phage P2 Enables Delivery of Antimicrobials into 
            Multiple Gut Pathogens.
  JOURNAL   ACS Synth Biol. 2023 Feb 17;12(2):596-607. doi: 
            10.1021/acssynbio.2c00615. Epub 2023 Feb 2.
  PUBMED    36731126
REFERENCE   2  (bases 1 to 6869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00615"; journalName: "ACS Synth Biol"; date: 
            "2023-02-17- 17"; volume: "12"; issue: "2"; pages: "596-607"
COMMENT     Sequence Label: pP2-P1(S')
FEATURES             Location/Qualifiers
     source          1..6869
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    26..47
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             3417..3434
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3487..3505)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      3501..3548
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    3580..3600
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      3601..3658
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     CDS             complement(3961..4911)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      4959..5181
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(5673..5767)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(5797..6588,6589..6657))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIQYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6420..6439
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6658..6762)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6827..6845
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(join(6849..6869,1..23))
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagagtac gctctccttg
       61 aatgttgtct ggtagttcta caaatgaatc cagatagcat aacttttata tattgtgcaa
      121 tctcacatgc atgagcataa aattcagaac cgttatcacc actgccggtg cagcaaagct
      181 ggcagcggca accgcgccgg gagggcggaa ggtcaacatt accacgatgg ccgtcgggga
      241 tggcggtggt aaattgcctg tcccggatgc cggacagacc gggcttatcc atgaagtctg
      301 gcgacatgcg ctgaacaaaa tcagccagga caaacgaaac agtaattata ttatcgccga
      361 gctggttatt ccgccggagg tgggcggttt ctggatgcgt gagcttggcc tgtacgatga
      421 tgcgggaacg ttaattgccg tggcgaacat ggccgaaagc tataagccag cccttgccga
      481 aggctcagga cgttcgcaga cctgtcgcat ggtcatcatc gtcagcagtg tggcctcagt
      541 ggagctgacc attgacacca caacggtgat ggcgacgcag gattacgttg atgacaaaat
      601 tgcagagcac gaacagtcac gacgtcaccc ggacgcctcg ctgacagcaa aaggttttac
      661 tcagttaagc agtgcgacca acagcacgtc tgaaacactg gccgcaacgc cgaaagcggt
      721 aaaggccgcg tatgacctgg ctaacgggaa atataccgca caggacgcca ccacagcgcg
      781 aaaaggcctt gtccagctta gtagcgccac caacagcacg tctgaaacgc tcgccgcaac
      841 accaaaagcc gttaagacgg taatggatga aacgaacaaa aaagcgccat taaacagccc
      901 tgcactgacc ggaacgccaa cgacgccaac tgcgcgacag ggaacgaata atactcagat
      961 cgcaaacacg gctttcgtta tggccgcgat tgccgccctt gtagactcgt cgcctgacgc
     1021 actgaatacg ctgaacgagc tggcggcggc gctgggcaat gacccgaatt ttgctaccac
     1081 catgactaat gcgcttgcgg gtaagcaacc gaaagatgct accctgacgg cgctggcggg
     1141 gcttgctact gcggcagaca ggtttccgta ttttacgggg aatgatgttg ccagcctggc
     1201 gaccctgaca aaagtcgggc gggatattct ggctaaatcg agcattcttg ctgttatcca
     1261 ataccttggt ttaagagaac tcggtaccag cggtgaaaag atccccctgt tgagcacggc
     1321 taacacatgg agtgcacgcc agactttcaa cggcgggatc accggggcgc tgacagggaa
     1381 cgccgatacc gcaacgaaat tgaaaacagc cagaaacatt aatggcgtca ggttcgatgg
     1441 ttctggtgac attaatatca atactctggt atcgcgcggt cgcgtaacgg ccctggaggc
     1501 gaatgcacag ggaacatccg ggattcagct gtatgaggca tacaacaatg gctacccttc
     1561 cccctatggc aatgtgcttc accttaaagg tgccaccgct gctggcgaag gtgagttatt
     1621 cattggctgg agtggcacga gcggtgccca tgcgcccgta catatccgtt cgcggcggga
     1681 tactgattct gccaactggt ctgaatgggc gcaggtctat acgtcaaaag attcaattcc
     1741 cggcgtcaat gccaaagggg atcaggatac ctctggtaat gcggctacag cgaccaagtt
     1801 gcagacagca tgtactatca acggtgtctc gtttgatggt tctaaaaata ttgagctaac
     1861 ggctgaaaat ttaaatcttg agcgaacagt agaattagcc gctgggtcat tgcagaaaaa
     1921 tcagaacggc gcggatattc ctggaaaaga taccttcaca aaaaatattg gtgcatgtcg
     1981 cgcttttcac agttctatta gtacaggtgc agggaactgg acaacggcac aattgattga
     2041 atggctggat tctcaagggg cattcaatca cccatactgg atgtgcaaat gttcatggtc
     2101 gtacggcaat aataaaatta taaccgatac tggctgtgga actattcatc ttgcaggttg
     2161 cgttattgag gttatgggta ataaaggtgc catgaccatc cgtgtaacaa caccaagcac
     2221 ttccagcggt ggcggaatca ctaacgctca attcacttat attaatcatg gtgatgctta
     2281 cgctcctggc tggcgacgag actacaacac gaaaaacctg caacctgcat ttgctttagg
     2341 gcagacagga aacagggttg caaatgataa agctgttggc tggaactgga atagcggtgt
     2401 ttatgatgca gacctaaaag gcgcatcaac attaattctt catttcaata tgaacgcggg
     2461 tagctgcccg gctgtacaat tacgcgtgaa ttataagaac ggcggtattt attatcgttc
     2521 agcgcgtgat ggttatggat ttgaggctga ctggtcagag ttttacacca caacccgcaa
     2581 accctctgcg ggggatgttg gtgcatatac gcaggcagaa tgtaactcaa ggtttattac
     2641 aggtattcgc ctgggcggtc tgtcatctgt ccagacatgg aatggccccg gctggtctga
     2701 caggtcaggt tatgtcgtta cgggttcagt taacgggaac cgtgatgaat taattgatac
     2761 aacacaggca aggccaattc agtattgcat taatgggacg tggtataacg cggggagtat
     2821 ttaattatga tgcacttaag aaatattaca gctggcaacc ctaaaacaaa agagcaatac
     2881 cagctaacga aacaatttaa catcaaatgg ctttatacag aggatggaaa aaactggtat
     2941 gaggaacaaa agaatttcca gtatgatacg ttgaaaatgg cctatgacca caacggcgtt
     3001 attatttgta ttgaaaagga tgtttcagca attaatccag aaggcgcaag cgtcgttgaa
     3061 ttacctgata ttacagcaaa tcgccgggct gatatttctg gtaaatggat gttcaaagat
     3121 ggcgtagtgg taaagcgaac ttataccgag gaagagcaga ggcaacaagc ggaaaatgaa
     3181 aagcaaagtc tgctacagct cgtcagggat aaaacccagc tatgggactc acagctacgg
     3241 ctgggtatca tttccgccga gaataagcag aaattaaccg agtggatgct ctttgcgcag
     3301 aaagtcgaat ccacagacac ctccagccta ccagtaacat ttcccgaaca acctgaatga
     3361 gacaaggcaa ggatccgaat tcgagctccg tcgacaagct tgcggccgca ctcgagcacc
     3421 accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg
     3481 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
     3541 gttttttgct gaaaggagga actatatccg gattaataat actagtagcg gccgctgcag
     3601 tccggcaaaa aagggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt
     3661 acttcgcgtt atgcaggctt cctcgctcac tgactcactg cgctcggtcg ttcggctgcg
     3721 gcgagcggta tcagctcact caaaggcggt aatctcgagg ttacattgtc gatctgttca
     3781 tggtgaacag ctttgaatgc accaaaaact cgtaaaagct ctgatgtatc tatctttttt
     3841 acaccgtttt catctgtgca tatggacagt tttccctttg atatgtaacg gtgaacagtt
     3901 gttctacttt tgtttgttag tcttgatgct tcactgatag atacaagagc cataagaacc
     3961 tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag
     4021 ccatgagaac gaaccattga gatcatactt actttgcatg tcactcaaaa attttgcctc
     4081 aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt
     4141 atgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg
     4201 gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa
     4261 cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa
     4321 atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt
     4381 ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt
     4441 tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc
     4501 aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg
     4561 caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt
     4621 ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat
     4681 cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat
     4741 ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat
     4801 cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc
     4861 atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa
     4921 ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat gataattaca
     4981 tgtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact
     5041 tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta
     5101 tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga
     5161 tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc
     5221 gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac
     5281 cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga
     5341 gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg
     5401 gtaaatggca ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag
     5461 aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct
     5521 gactttttgc tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta
     5581 tcccgtgaca ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca
     5641 ggcttacccg tcttactgtc cctagtgctt ggattctcac caataaaaaa cgcccggcgg
     5701 caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac
     5761 aggagtccaa gcgagctcgt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     5821 acctatctca gctatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     5881 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     5941 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     6001 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     6061 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     6121 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     6181 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     6241 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     6301 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     6361 gtcattctga gaatattgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     6421 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     6481 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6541 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     6601 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     6661 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     6721 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc ccatggtgcc
     6781 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     6841 gaggcagaat ttcagataaa aaaaatcct
//
