LOCUS       sequence-393315-        5550 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5550)
  TITLE     Davidson mEmerald
REFERENCE   2  (bases 1 to 5550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEmerald-Tropomyosin-N-14
FEATURES             Location/Qualifiers
     source          1..5550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             74..868
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             74..868
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(128..147)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     738..757
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1056..1075)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1100..1147
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1476..2064
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1965..1984
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2239..2542
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2543..2746
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2697..2717
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      2775..2784
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3669..3678
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3675..3677,3678..3680,3681..4394)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3720..3741)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3981..4000)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4328..4349
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4516..4637
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4553..4572)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4607..4626
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4644..5099)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4781..4802)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4993..5012
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5126..5230
                     /gene="bla"
                     /label=AmpR promoter
     promoter        join(5232..5550,1..39)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5232..5423
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5255..5275)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      join(5440..5550,1..25)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg
       61 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
      121 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
      181 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
      241 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
      301 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
      361 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
      421 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
      481 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
      541 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
      601 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
      661 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
      721 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
      781 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
      841 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
      901 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
      961 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     1021 ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg
     1081 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg
     1141 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
     1201 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
     1261 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt
     1321 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     1381 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     1441 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     1501 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1561 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1621 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1681 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1741 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1801 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     1861 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     1921 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     1981 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     2041 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     2101 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     2161 cgtattaccg ccatgcatta gttattaata gtaatcaatt acggggtcat tagttcatag
     2221 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     2281 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     2341 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     2401 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     2461 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     2521 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     2581 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     2641 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     2701 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg
     2761 tcagatccgc tagcgccacc atggacgcca tcaagaagaa gatgcagatg ctgaagctcg
     2821 acaaggagaa cgccttggat cgagctgagc aggcggaggc cgacaagaag gcggcggaag
     2881 acaggagcaa gcagctggaa gatgagctgg tgtcactgca aaagaaactc aagggcaccg
     2941 aagatgaact ggacaaatac tctgaggctc tcaaagatgc ccaggagaag ctggagctgg
     3001 cagagaaaaa ggccaccgat gctgaagccg acgtagcttc tctgaacaga cgcatccagc
     3061 tggttgagga agagttggat cgtgcccagg agcgtctggc aacagctttg cagaagctgg
     3121 aggaagctga gaaggcagca gatgagagtg agagaggcat gaaagtcatt gagagtcgag
     3181 cccaaaaaga tgaagaaaaa atggaaattc aggagatcca actgaaagag gcaaagcaca
     3241 ttgctgaaga tgccgaccgc aaatatgaag aggtggcccg taagctggtc atcattgaga
     3301 gcgacctgga acgtgcagag gagcgggctg agctctcaga aggcaaatgt gccgagcttg
     3361 aagaagaatt gaaaactgtg acgaacaact tgaagtcact ggaggctcag gctgagaagt
     3421 actcgcagaa ggaagacaga tatgaggaag agatcaaggt cctttccgac aagctgaagg
     3481 aggctgagac tcgggctgag tttgcggaga ggtcagtaac taaattggag aaaagcattg
     3541 atgacttaga agacgagctg tacgctcaga aactgaagta caaagccatc agcgaggagc
     3601 tggaccacgc tctcaacgat atgacttcca tatccggcgg tagcggcggt agcggggatc
     3661 caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     3721 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     3781 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     3841 tgccctggcc caccctcgtg accaccttga cctacggcgt gcagtgcttc gcccgctacc
     3901 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     3961 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     4021 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     4081 acatcctggg gcacaagctg gagtacaact acaacagcca caaggtctat atcaccgccg
     4141 acaagcagaa gaacggcatc aaggtgaact tcaagacccg ccacaacatc gaggacggca
     4201 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     4261 tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc
     4321 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     4381 agctgtacaa gtaagcggcc gcgactctag atcataatca gccataccac atttgtagag
     4441 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     4501 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     4561 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     4621 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     4681 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     4741 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     4801 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     4861 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     4921 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     4981 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     5041 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     5101 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     5161 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5221 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     5281 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5341 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5401 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     5461 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     5521 gccgcctcgg cctctgagct attccagaag
//
