LOCUS       sequence-393322-        4693 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 598, Emission: 625.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Kremers GJ, Hazelwood KL, Murphy CS, Davidson MW, Piston DW
  TITLE     Photoconversion in orange and red fluorescent proteins.
  JOURNAL   Nat Methods. 2009 May;6(5):355-8. doi: 10.1038/nmeth.1319. Epub 2009
            Apr 12.
  PUBMED    19363494
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1319"; journalName: "Nat Methods"; date: "2009-05"; 
            volume: "6"; issue: "5"; pages: "355-8"
COMMENT     Sequence Label: mRaspberry-N1
FEATURES             Location/Qualifiers
     source          1..4693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             56..850
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             56..850
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(110..129)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     720..739
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1038..1057)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1082..1129
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1458..2046
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1947..1966
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2221..2524
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2525..2728
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2679..2699
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    2751..2831
                     /label=MCS
                     /note="multiple cloning site"
     CDS             2839..3519
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mRaspberry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQCMYGSKGYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     complement(2972..2990)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3240..3259
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3434..3457
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    3641..3762
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3678..3697)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3732..3751
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3769..4224)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3906..3927)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4118..4137
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4251..4355
                     /gene="bla"
                     /label=AmpR promoter
     promoter        join(4357..4693,1..21)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4357..4548
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4380..4400)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      join(4565..4693,1..7)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat
       61 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
      121 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
      181 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
      241 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
      301 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
      361 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
      421 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
      481 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
      541 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
      601 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
      661 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
      721 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
      781 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
      841 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
      901 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
      961 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     1021 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     1081 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
     1141 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     1201 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     1261 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag
     1321 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     1381 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1441 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1501 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1561 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1621 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1681 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1741 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1801 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1861 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1921 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1981 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2041 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2101 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
     2161 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     2221 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     2281 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     2341 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     2401 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     2461 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     2521 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     2581 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     2641 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     2701 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
     2761 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
     2821 gatccaccgg tggctaccat ggtgagcaag ggcgaggagg tcatcaagga gttcatgcgc
     2881 ttcaaggtgc gcatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     2941 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     3001 ctgcccttcg cctgggacat cctgtcccct cagtgcatgt acggctccaa gggctacgtg
     3061 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
     3121 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
     3181 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
     3241 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
     3301 gacggcgccc tgaagggcga gatgaagatg aggctgaagc tgaaggacgg cggccactac
     3361 gacgccgagg tcaagaccac ctacatggcc aagaagcccg tgcagctgcc cggcgcctac
     3421 aagaccgaca tcaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
     3481 tacgagcgcg ccgagggccg ccactccacc ggcgcctaag cggccgcgac tctagatcat
     3541 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     3601 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     3661 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     3721 gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg
     3781 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     3841 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     3901 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     3961 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     4021 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     4081 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     4141 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     4201 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4261 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4321 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
     4381 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4441 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     4501 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     4561 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     4621 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     4681 aggaggcttt ttt
//
