LOCUS       sequence-393327-        7054 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 
            / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7054)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 7054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: mEos2-Integrin-Beta3-N-18
FEATURES             Location/Qualifiers
     source          1..7054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             75..869
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             75..869
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(129..148)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     739..758
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1057..1076)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1101..1148
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1477..2065
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1966..1985
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2240..2543
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2544..2747
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2698..2718
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             5219..5899
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    6021..6142
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6058..6077)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6112..6131
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6149..6604)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6286..6307)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6498..6517
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6631..6735
                     /gene="bla"
                     /label=AmpR promoter
     promoter        join(6737..7054,1..40)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6737..6928
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6760..6780)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      join(6945..7054,1..26)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat
       61 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
      121 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
      181 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
      241 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
      301 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
      361 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
      421 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
      481 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
      541 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
      601 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
      661 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
      721 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
      781 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
      841 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
      901 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
      961 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     1021 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc
     1081 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc
     1141 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
     1201 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
     1261 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg
     1321 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     1381 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1441 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1501 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1561 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     1621 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     1681 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     1741 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     1801 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     1861 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     1921 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     1981 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2041 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2101 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     2161 ccgtattacc gccatgcatt agttattaat agtaatcaat tacggggtca ttagttcata
     2221 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     2281 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     2341 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     2401 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     2461 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     2521 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     2581 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     2641 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     2701 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt ttagtgaacc
     2761 gtcagatccg ctagcgctac cggactcaga tctcgagacc gccatgcgag cgcagtggcc
     2821 gggacaactc tgggccgctc tgctggcgct gggggcgctg gcgggcgttg ttgttggaga
     2881 gtccaacatc tgtaccacac gaggcgtgaa ctcctgccag cagtgtctgg ctgtgagtcc
     2941 tgtgtgtgcc tggtgctcag atgagacttt gtctcagggc tcaccccgat gtaacctgaa
     3001 ggagaacctg ctgaaggaca attgtgctcc agagtctatt gagttcccag tcagtgaggc
     3061 ccagatcctg gaggctaggc cactcagcag caagggctct ggaagcagcg cccagatcac
     3121 tcaagtcagc cctcagagga ttgcccttcg actacggcca gatgattcga agatcttctc
     3181 acttcaagtg cggcaggtgg aggattaccc cgtggacatc tactacttga tggacctgtc
     3241 tttctccatg aaggatgatc tgtccagcat ccagaccctg ggtaccaagt tggcctctca
     3301 gatgcgcaag cttactagca accttcggat cggctttggg gccttcgtgg acaagcctgt
     3361 atcgccgtac atgtacatct ccccaccaca ggcaatcaaa aacccctgtt acaatatgaa
     3421 gaatgcctgc ttgcccatgt ttggctacaa acacgtgctg acgctaaccg accaggtgtc
     3481 ccgcttcaat gaagaagtga agaaacagag cgtgtcccgt aatcgagatg ccccagaggg
     3541 cggctttgac gccatcatgc aggctacagt atgtgatgaa aaaattggct ggaggaatga
     3601 cgcatcccat ttgctagtgt ttaccacgga tgccaagacc catattgccc tggatggaag
     3661 gctggcaggc attgtcctgc ccaatgatgg gcactgtcac attggcaccg acaaccacta
     3721 ctctgcctcc actaccatgg actacccatc tctggggctg atgactgaga aactatccca
     3781 gaaaaacatt aacttgatct ttgcagtgac tgaaaatgtc gtcagccttt accagaatta
     3841 tagtgagctc attcctggga ccacagtggg agtcctgtct gatgactcaa gcaacgtcct
     3901 ccagctcatt gttgatgctt acgggaaaat ccgctctaaa gtggagctgg aagtacgtga
     3961 cctgccggaa gaactgtcac tgtccttcaa tgccacctgc ctcaacaacg aggttatccc
     4021 gggcctcaag tcttgtgtgg gcctcaagat tggagacacg gtgagcttta gtatcgaggc
     4081 caaggtgcgt ggctgccccc aggagaagga gcagtctttc actatcaagc ctgtgggctt
     4141 taaggacagc ctcaccgtcc aggtgacctt cgactgtgac tgtgcctgcc aggcctttgc
     4201 ccagccttcc agcccacgct gcaacaatgg gaacgggact tttgagtgtg gggtgtgccg
     4261 ctgtgaccag ggctggctgg ggtccatgtg tgagtgctct gaggaggatt accgaccctc
     4321 tcagcaggaa gagtgcagcc ccaaggaggg ccagcccatc tgcagccagc ggggagagtg
     4381 cctctgtggc cagtgtgtct gccatagcag cgacttcggc aagatcactg gcaagtactg
     4441 tgagtgcgat gacttctcct gcgtccgcta caaaggggag atgtgttccg gccatgggca
     4501 atgtaactgt ggggactgcg tgtgtgactc ggactggact ggctactact gcaactgtac
     4561 tacacgcact gacacctgca tgtccaccaa tgggctgctg tgcagcggcc ggggcaactg
     4621 cgagtgcggc agctgtgtgt gcgtccagcc aggctcctat ggagacacct gtgagaagtg
     4681 ccccacctgc ccagatgcct gctcctttaa gaaggagtgt gtggagtgta agaagttcaa
     4741 ccggggaacg ctccatgaag aaaacacctg cagccgctac tgccgggacg acatcgagca
     4801 ggtgaaagag ctgacggata ctggcaaaaa cgccgtgaat tgtacctaca agaacgagga
     4861 tgactgtgtc gtcagattcc agtactacga agacaccagt gggagggcag tcctctatgt
     4921 ggtggaagag cctgagtgtc ccaagggtcc tgatatcctg gtggtcctgc tgtcagtgat
     4981 gggggccatc ctgctcattg gccttgctac tctgctcatc tggaagctac tcatcaccat
     5041 ccatgaccgg aaggaatttg ctaaatttga ggaagaacga gccagagcca agtgggacac
     5101 agcaaacaac ccgctgtata aagaggccac ctccaccttc accaatatca cctaccgggg
     5161 gactggtagc gcatccgggg gtgcgtcagg cggcagcgga tctggaccgg tcgccaccat
     5221 gagtgcgatt aagccagaca tgaagatcaa actccgtatg gaaggcaacg taaacgggca
     5281 ccactttgtg atcgacggag atggtacagg caagcctttt gagggaaaac agagtatgga
     5341 tcttgaagtc aaagagggcg gacctctgcc ttttgccttt gatatcctga ccactgcatt
     5401 ccattacggc aacagggtat tcgccaaata tccagacaac atacaagact attttaagca
     5461 gtcgtttcct aaggggtatt cgtgggaacg aagcttgact ttcgaagacg ggggcatttg
     5521 cattgccaga aacgacataa caatggaagg ggacactttc tataataaag ttcgatttta
     5581 tggtaccaac tttcccgcca atggtccagt tatgcagaag aagacgctga aatgggagcc
     5641 ctccactgag aaaatgtatg tgcgtgatgg agtgctgacg ggtgatattc atatggcttt
     5701 gttgcttgaa ggaaatgccc attaccgatg tgacttcaga actacttaca aagctaagga
     5761 gaagggtgtc aagttaccag gctaccactt tgtggaccac tgcattgaga ttttaagcca
     5821 tgacaaagat tacaacaagg ttaagctgta tgagcatgct gttgctcatt ctggattgcc
     5881 tgacaatgcc agacgataag cggccgcgac tctagatcat aatcagccat accacatttg
     5941 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     6001 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     6061 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     6121 ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc
     6181 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     6241 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     6301 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     6361 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     6421 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     6481 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     6541 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     6601 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     6661 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     6721 tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
     6781 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     6841 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     6901 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     6961 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     7021 ccgaggccgc ctcggcctct gagctattcc agaa
//
