LOCUS       sequence-393332-        4908 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Bashiri G, Rehan AM, Greenwood DR, Dickson JM, Baker EN
  TITLE     Metabolic engineering of cofactor F420 production in Mycobacterium 
            smegmatis.
  JOURNAL   PLoS One. 2010 Dec 29;5(12):e15803.
  PUBMED    21209917
REFERENCE   2  (bases 1 to 4908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2010-12-29"; volume: "5(12)"; pages: "e15803"
COMMENT     Sequence Label: pYUB28b
FEATURES             Location/Qualifiers
     source          1..4908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    80..104
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             119..141
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             160..177
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             187..204
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             208..240
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             289..306
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(359..377)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      373..420
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      457..885
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(544..563)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     754..775
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     rep_origin      complement(2988..3576)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3068..3087)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
       61 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
      121 tgtttaactt taagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc
      181 agcggcctgg tgccgcgcgg cagccatatg gctagcatga ctggtggaca gcaaatgggt
      241 cgggatccga attcgagctc cgtcgacaag cttgcggccg cactcgagca ccaccaccac
      301 caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
      361 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
      421 ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa
      481 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
      541 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
      601 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
      661 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
      721 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
      781 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
      841 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatatcta
      901 gatttaaaga tctggtaccg cggccgcgcc acggatgcca ccacaggcac tacaacggag
      961 ttcgccacgt acatcaccac aaccaccgat tctggcggtg agctccccga tattcagcgg
     1021 aaatggcttg gtatcgacca agattcgtag aaccccgtct cgtctggctg gtattcaaaa
     1081 cgggcgcaac gaaacacgca acgagacagg catggcccaa accagaaaac tagcgtctac
     1141 caggactttt acctgtccga cccgttgcaa cggaaccccc cacggaaccc ccgcgacacc
     1201 cgctccccaa ttgcgttaga acagcggtgg attgtcggct tcgttgtggg ccttttgagc
     1261 cgcttcctgt tctgccgcac gctctttcct cgcccgatag ccgagtcgct taacggtgtc
     1321 cagatgcagc ccgaaatgtt tggccgtttg cggccaagag tggccctcgt cgtcgtgata
     1381 ggcgcggatg cgttcgcggc gtgcagcctg ctcggcgagc cactcgctgc gttcctgcgc
     1441 cacgagccgg acgacgtggc gttcggatag tccggtgatt cgagcgcctt cggcggcggt
     1501 cacgcgccgc tttttgcgga cagtcggctg ccggttgtag ccgtcgctgt agccgtcgct
     1561 catagcaatg cctccatgca tggctgacgc ggactttgcg cgccgcgcaa ctgtgctcgc
     1621 cgccgtgcgc gctgctgcgc ccttccgcga gatggccgac tggcgcgcac tgagtgtggc
     1681 ctcgtagacc acgatcccgt ccgcccaaat gcgcgacttg gttgtgatcc aacgccaaat
     1741 gctgttggcg atggcgcgga cctcgctgtc cggtagcggt ccgggacaca cgtcgttgca
     1801 cggaaattcg gcgtttcgcg cgtggcactc ggcatagatc gcgcggccga gtccgtccac
     1861 gttccgggtc ggcaggtaga tccgcatgag ggcgggacga taggcccaca acctgacgga
     1921 atcgaacagt gcgcaattcc gccctagcgg cgtcggagcc gctttgtacg tggtctgctg
     1981 acgccagcgc ggcggtggca tgttcgcgcc gagctcggcc tcgatgtggc tgagtgtgta
     2041 gagatctgag tggagccatt ccgtttccca ggcgatgtgg ccggggtttt tggtcatgag
     2101 gcctgagtaa ctgcggtcgc catcgacggc gcgccgaagg ccttcggcgc acgccgccat
     2161 gtatgcgagc ggcttacgcc gcgcgtattc ggtgcgtgga acaggggcgt tgagtgccca
     2221 cactgcgtgt gcgtggccgt tggcgcgatt gcccacgatc gcgttgggca gcggatggga
     2281 cccccgggcg ctgagcgctc ggagcgctgc gtctggatgg tctacgtcca cgaccagcag
     2341 gtttgccagc gctgttgggt tcgcctcgat gtaccggcgg cctagggccg acgcgcggct
     2401 ttggcggtag atcccctcga gcagatcgtc gcttgccagc ggccagtacg gcagccagag
     2461 ctgctcaaat tcgtcggcga cgtggctcac gcttggtagt agaccacgat taatcaccgg
     2521 tgtatggtcc gacacgagct ccaagtcaga tatttcgctg aggggccacc ccacaactgc
     2581 acactccccc gctctcccgt cgagccctgg tggtggaaca ccagcgacag ccgagcaccc
     2641 ccaaccacct gtaccaacca ggaggaacac atgcgtcgtt tcgaggacgt ttccgggccg
     2701 ctgagagccg ctgtggcggc cgtacacgcc gccttagacc cgttagaccc cctgccgcct
     2761 gaatgcgcgg gtacgagcca cacagcgccc gaacttacgg agctggtggg ctcacgcgtg
     2821 cggccgcacg cgttgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2881 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     2941 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     3001 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     3061 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     3121 accctgccgc ttaccggata cctgtccgcc tttctcccct cgggaagcgt ggcgctttct
     3181 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3241 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3301 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3361 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3421 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3481 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3541 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3601 gggtctgacg ctcatggaac tagagcatga gattatcaaa aaggatcttc acctagatcc
     3661 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3721 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     3781 ccatagttgc ctgactcccc gacgtggccg accagcccgt catcgtcaac gcctgatccg
     3841 cggtgcggac aggccgtgtc gcgaccggcc gtgcggaatt aagccggccc gtaccctgtg
     3901 aatagaggtc cgctgtgaca caagaatccc tgttacttct cgaccgtatt gattcggatg
     3961 attcctacgc gagcctgcgg aacgaccagg aattctggga gccgctggcc cgccgagccc
     4021 tggaggagct cgggctgccg gtgccgccgg tgctgcgggt gcccggcgag agcaccaacc
     4081 ccgtactggt cggcgagccc ggcccggtga tcaagctgtt cggcgagcac tggtgcggtc
     4141 cggagagcct cgcgtcggag tcggaggcgt acgcggtcct ggcggacgcc ccggtgccgg
     4201 tgccccgcct cctcggccgc ggcgagctgc ggcccggcac cggagcctgg ccgtggccct
     4261 acctggtgat gagccggatg accggcacca cctggcggtc cgcgatggac ggcacgaccg
     4321 accggaacgc gctgctcgcc ctggcccgcg aactcggccg ggtgctcggc cggctgcaca
     4381 gggtgccgct gaccgggaac accgtgctca ccccccattc cgaggtcttc ccggaactgc
     4441 tgcgggaacg ccgcgcggcg accgtcgagg accaccgcgg gtggggctac ctctcgcccc
     4501 ggctgctgga ccgcctggag gactggctgc cggacgtgga cacgctgctg gccggccgcg
     4561 aaccccggtt cgtccacggc gacctgcacg ggaccaacat cttcgtggac ctggccgcga
     4621 ccgaggtcac cgggatcgtc gacttcaccg acgtctatgc gggagactcc cgctacagcc
     4681 tggtgcaact gcatctcaac gccttccggg gcgaccgcga gatcctggcc gcgctgctcg
     4741 acggggcgca gtggaagcgg accgaggact tcgcccgcga actgctcgcc ttcaccttcc
     4801 tgcacgactt cgaggtgttc gaggagaccc cgctggatct ctccggcttc accgatccgg
     4861 aggaactggc gcagttcctc tgggggccgc cggacaccgc ccccggcg
//
