LOCUS       sequence-393372-        5016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Mannosidase/Golgi, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-MannII-N-10
FEATURES             Location/Qualifiers
     source          1..5016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             92..886
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             92..886
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(146..165)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     756..775
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1074..1093)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1118..1165
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1494..2082
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1983..2002
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2257..2560
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2561..2764
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2715..2735
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3159..3168
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3165..3875
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     polyA_signal    4000..4121
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4037..4056)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4091..4110
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4128..4583)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4265..4286)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4477..4496
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4610..4714
                     /gene="bla"
                     /label=AmpR promoter
     promoter        join(4716..5016,1..57)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4716..4907
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4739..4759)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      join(4924..5016,1..43)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat
       61 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
      121 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
      181 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
      241 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
      301 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
      361 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
      421 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
      481 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
      541 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
      601 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
      661 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
      721 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
      781 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
      841 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
      901 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
      961 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     1021 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca
     1081 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
     1141 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
     1201 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca
     1261 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc
     1321 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa
     1381 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     1441 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     1501 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     1561 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     1621 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     1681 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1741 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1801 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     1861 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     1921 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     1981 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2041 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     2101 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     2161 cctgattctg tggataaccg tattaccgcc atgcattagt tattaatagt aatcaattac
     2221 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     2281 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     2341 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     2401 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     2461 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     2521 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     2581 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     2641 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     2701 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     2761 agctggttta gtgaaccgtc agatccgcta gcgccaccat gaagttaagt cgccagttca
     2821 ccgtgtttgg cagcgcgatc ttctgcgtcg taatcttctc actctacctg atgctggaca
     2881 ggggtcactt ggactaccct cggggcccgc gccaggaggg ctcctttccg cagggccagc
     2941 tttcaatatt gcaagaaaag attgaccatt tggagcgttt gctcgctgag aacaacgaga
     3001 tcatctcaaa tatcagagac tcagtcatca acctgagcga gtctgtggag gacggcccgc
     3061 gggggtcacc aggcaacgcc agccaaggct ccatccacct ccactcgcca cagttggccc
     3121 tgcaggctga ccccagctcc ggatcggatc caccggtcgc caccatggtg tctaagggcg
     3181 aagagctgat taaggagaac atgcacatga agctgtacat ggagggcacc gtggacaacc
     3241 atcacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc cagaccatga
     3301 gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg gctactagct
     3361 tcctctacgg cagcaagacc ttcatcaacc acacccaggg catccccgac ttcttcaagc
     3421 agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaagac gggggcgtgc
     3481 tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac gtcaagatca
     3541 gaggggtgaa cttcacatcc aacggccctg tgatgcagaa gaaaacactc ggctgggagg
     3601 ccttcaccga gacgctgtac cccgctgacg gcggcctgga aggcagaaac gacatggccc
     3661 tgaagctcgt gggcgggagc catctgatcg caaacgccaa gaccacatat agatccaaga
     3721 aacccgctaa gaacctcaag atgcctggcg tctactatgt ggactacaga ctggaaagaa
     3781 tcaaggaggc caacaacgag acctacgtcg agcagcacga ggtggcagtg gccagatact
     3841 gcgacctccc tagcaaactg gggcacaagc ttaattaagc ggccgcgact ctagatcata
     3901 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     3961 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     4021 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     4081 cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt
     4141 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     4201 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     4261 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     4321 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     4381 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     4441 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     4501 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     4561 taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4621 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4681 ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg
     4741 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4801 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     4861 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     4921 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     4981 ttttttattt atgcagaggc cgaggccgcc tcggcc
//
