LOCUS       sequence-393403-         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for the expression of the basic region and leucine zipper 
            domain of the Saccharomyces cerevisiae transcription factor GCN4 in 
            bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Weiss MA, Ellenberger T, Wobbe CR, Lee JP, Harrison SC, Struhl K
  TITLE     Folding transition in the DNA-binding domain of GCN4 on specific 
            binding to DNA.
  JOURNAL   Nature. 1990 Oct 11;347(6293):575-8. doi: 10.1038/347575a0.
  PUBMED    2145515
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "1990-10-11- 11"; volume: "347"; issue: "6293"; 
            pages: "575-8"
COMMENT     Sequence Label: pGCNK58
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..21
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             132..188
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     primer_bind     complement(278..296)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      292..339
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(705..710,711..727,728..733))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
ORIGIN
        1 ttgtttaact ttaagaagga gatatacata tgaaagatcc ggcggcgctg aaacgcgcgc
       61 gtaacacaga agctgcgcgt cgtagccgtg ctcgtaaact gcagcgtatg aagcagctcg
      121 aggataaagt ggaagaactc ttaagcaaaa actaccacct ggaaaacgaa gtggcacgtc
      181 tgaaaaagct tgtgggtgag cgttaatgag ctctcgagtc tagaatcgga tccggctgct
      241 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
      301 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
      361 ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag
      421 cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc
      481 gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct
      541 gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc
      601 tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca
      661 cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat
      721 gataagctgt caaacatgag aattcttga
//
