LOCUS       sequence-393427-        5414 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV for Chemogenetic plasmids.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Magnus CJ, Lee PH, Bonaventura J, Zemla R, Gomez JL, Ramirez MH, Hu 
            X, Galvan A, Basu J, Michaelides M, Sternson SM
  TITLE     Ultrapotent chemogenetics for research and potential clinical 
            applications.
  JOURNAL   Science. 2019 Mar 14. pii: science.aav5282. doi: 
            10.1126/science.aav5282.
  PUBMED    30872534
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2019 Mar 14. pii: science.aav5282. doi: 10.1126/science.aav5282."
COMMENT     Sequence Label: pAAV Syn intron Psam4GlyR m3m4 HA
FEATURES             Location/Qualifiers
     source          1..5414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..39
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(258..362)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(388..843)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(525..546)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     737..756
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   915..1044
                     /label=AAV2 ITR
     intron          1637..1769
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1752..1771
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             2912..2938
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(3193..3213)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     polyA_signal    3404..3524
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3415..3434)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   3686..3815
                     /label=AAV2 ITR
     rep_origin      complement(4052..4640)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4132..4151)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4811..5414,1..188,189..257))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
       61 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
      121 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
      181 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
      241 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
      301 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
      361 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
      421 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
      481 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
      541 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
      601 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
      661 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
      721 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
      781 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
      841 cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
      901 cgctattacg ccagctgcgc gctcgctcgc tcactgaggc cgcccgggca aagcccgggc
      961 gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg
     1021 ccaactccat cactaggggt tccttgtagt taatgattaa cccgccatgc tacttatcta
     1081 cgtagccatg ctctaggaag atccgagctc cagtgtgctg gaattcgccc ttttcagtat
     1141 ttaaattgac gaccgacccc gacccactgg acaagcaccc aacccccatt ccccaaattg
     1201 cgcatcccct atcagagagg gggaggggaa acaggatgcg gcgaggcgcg tgcgcactgc
     1261 cagcttcagc accgcggaca gtgccttcgc ccccgcctgg cggcgcgcgc caccgccgcc
     1321 tcagcactga aggcgcgctg acgtcactcg ccggtccccc gcaaactccc cttcccggcc
     1381 accttggtcg cgtccgcgcc gccgccggcc cagccggacc gcaccacgcg aggcgcgaga
     1441 taggggggca cgggcgcgac catctgcgct gcggcgccgg cgactcagcg ctgcctcagt
     1501 ctgcggtggg cagcggagga gtcgtgtcgt gcctgagagc gcagctgtgc tcctgggcac
     1561 cgcgcagtcc gcccccgcgg ctcctggcca gaccacccct aggaccccct gccccaagtc
     1621 gcagccgttg gatcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
     1681 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
     1741 tgacatccac tttgcctttc tctccacagg tgatgatatc actagtgcta gcgccaccat
     1801 gcgctgttct ccaggcggcg tgtggctcgc cctggctgct tcccttctgc acgttagcct
     1861 gcagggtgag ttccagcgca aactgtataa ggagcttgtt aagaattata accccctgga
     1921 gcggccggtc gcaaatgatt cccagccact gacagtgtac ttcagcctct ccttgctgca
     1981 gatcatggac gtggatgaaa agaaccaggt gctgaccact aatatttggt tgcagatgtc
     2041 ctggaccgat cactacttgc agtggaatgt gagcgaatac ccaggtgtaa agactgtaag
     2101 attccctgac ggccaaatct ggaaaccaga tatcctgctg tacaacagcg cagacgaaag
     2161 gtttgatgca acatttcaca ccaacgtggg agtcaattct tcaggccact gcctgtacct
     2221 gccccctgga atcttcaagt cctcatgcta tatcgacgtc cgctggtttc ccttcgacgt
     2281 ccagcactgc aaactcaaat tcgggagctg gagctacggc ggatggagcc tggatctgca
     2341 aatgcaggag gctgacatct ctggttacat cccgaatggg gagtgggacc ttgtgggaat
     2401 ccccggtaaa agaagcgagc gattttatga atgctgcaaa gagcccttcc cagatgtcac
     2461 cttcacagtg accatgcgga gacgcatggg ttattatctg atccaaatgt atatcccaag
     2521 cttgcttata gtgattttgt catggatctc cttctggatt aatatggacg ccgctccagc
     2581 tagggtcgga ctgggcatca ccacagtgct gacaatgact actcagagct caggcagccg
     2641 agccagcttg cccaaggttt cttacgtgaa ggccatcgat atctggatgg ctgtctgcct
     2701 tctgtttgtc ttcagcgcac tgctggaata cgccgctgtc aattttgtgt ctcgacagca
     2761 taaagagctg ttgcggttca gaagaaaacg acgccaccac aaagaggatg aggcaggaga
     2821 aggacgcttc aactttagcg cctatggtat gggacctgct tgcctccagg ctaaagacgg
     2881 aatttccgtg aagggagcca acaatagcaa ctacccttat gatgtcccag actatgcaac
     2941 aaccaaccca ccccctgctc catctaagag cccggaggaa atgcgcaaac tctttattca
     3001 gagagcgaaa aagatcgaca aaatctcccg gatcggattc cccatggctt tcctgatttt
     3061 caacatgttt tattggatca tctacaagat tgtgcgaagg gaggacgtac acaaccagta
     3121 agcggcccgc gtctcgagta atcaacctct ggattacaaa atttgtgaaa gattgactgg
     3181 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     3241 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttagt
     3301 tcttgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     3361 gttgggcact gacaattccg tggtgcgctt cgagcagaca tgataagata cattgatgag
     3421 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga atttgtgatg
     3481 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     3541 ttcattttat gtttcaggtt cagggggaga tgtgggaggt tttttaaagc aagtaaaacc
     3601 tctacaaatg tggtaaaatc gataaggatc ttcctagagc atggctacgt agataagtag
     3661 catggcgggt taatcattaa ctacaaggaa cccctagtga tggagttggc cactccctct
     3721 ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt
     3781 gcccgggcgg cctcagtgag cgagcgagcg cgcagctgca ttaatgaatc ggccaacgcg
     3841 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     3901 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     3961 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     4021 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     4081 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     4141 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     4201 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     4261 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     4321 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     4381 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     4441 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     4501 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     4561 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     4621 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     4681 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     4741 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     4801 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     4861 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     4921 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     4981 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     5041 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     5101 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     5161 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     5221 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     5281 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     5341 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     5401 gaccgagttg ctct
//
