LOCUS       sequence-393447-        9205 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral transfer vector for the neuronal expression of 
            membrane-bound mScarlet (good visualization of neuronal processes).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9205)
  TITLE     Tools for analysing neuronal cultures in vitro
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9205)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-hSyn1-mScarlet/CAAX
FEATURES             Location/Qualifiers
     source          1..9205
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        111..490
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        492..690
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     645..665
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             708..888
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    935..1060
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1553..1786
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1971..2015
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2312..2429
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2484..2931
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      2962..2971
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2968..3663
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(3101..3119)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3369..3388
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3774..4362
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3827..3847)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4245..4256)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4365..4381)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4366..4382)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4883..5063
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5089..5106)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5095..5319
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5365..5793
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5452..5471)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5662..5683
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5802..5822)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5807..6136
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5987..6122
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6049..6068
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6184..6231
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6250..6624
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6754..6875
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6791..6810)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6845..6864
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6924..6940)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6924..6940)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6937..6959)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6948..6964
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6972..6978,6979..6996,6997..7002))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7017..7038
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7155..7172)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7326..7914)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7406..7425)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8085..8876,8877..8945))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8708..8727
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8946..9050)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9125..9144)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
       61 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
      121 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
      181 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      241 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      301 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      361 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      421 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      481 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      541 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      601 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      661 gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt
      721 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      781 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      841 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      901 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      961 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     1021 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     1081 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     1141 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     1201 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     1261 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     1321 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     1381 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     1441 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1501 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1561 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1621 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1681 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1741 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1801 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1861 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1921 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1981 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     2041 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     2101 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     2161 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     2221 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     2281 ttctgcagac aaatggcagt ttcatccaca attttaaaag aaaagggggg attggggggt
     2341 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     2401 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag
     2461 tttggttaat ccgagtgcgt atgagtgcaa gtgggtttta ggaccaggat gaggcggggt
     2521 gggggtgcct acctgacgac cgaccccgac ccactggaca agcacccaac ccccattccc
     2581 caaattgcgc atcccctatc agagaggggg aggggaaaca ggatgcggcg aggcgcgtgc
     2641 gcactgccag cttcagcacc gcggacagtg ccttcgcccc cgcctggcgg cgcgcgccac
     2701 cgccgcctca gcactgaagg cgcgctgacg tcactcgccg gtcccccgca aactcccctt
     2761 cccggccacc ttggtcgcgt ccgcgccgcc gccggcccag ccggaccgca ccacgcgagg
     2821 cgcgagatag gggggcacgg gcgcgaccat ctgcgctgcg gcgccggcga ctcagcgctg
     2881 cctcagtctg cggtgggcag cggaggagtc gtgtcgtgcc tgagagcgca gtcgctgcag
     2941 ggaactagtc ctaggggtac cgccaccatg gtgagcaagg gcgaggcagt gatcaaggag
     3001 ttcatgcggt tcaaggtgca catggagggc tccatgaacg gccacgagtt cgagatcgag
     3061 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     3121 ggtggccccc tgcccttctc ctgggacatc ctgtcccctc agttcatgta cggctccagg
     3181 gccttcacca agcaccccgc cgacatcccc gactactata agcagtcctt ccccgagggc
     3241 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg ccgtgaccgt gacccaggac
     3301 acctccctgg aggacggcac cctgatctac aaggtgaagc tccgcggcac caacttccct
     3361 cctgacggcc ccgtaatgca gaagaagaca atgggctggg aagcgtccac cgagcggttg
     3421 taccccgagg acggcgtgct gaagggcgac attaagatgg ccctgcgcct gaaggacggc
     3481 ggccgctacc tggcggactt caagaccacc tacaaggcca agaagcccgt gcagatgccc
     3541 ggcgcctaca acgtcgaccg caagttggac atcacctccc acaacgagga ctacaccgtg
     3601 gtggaacagt acgaacgctc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     3661 aagtccggac tcagatctcg agctcaagct tcgaattctg cagtcgacaa ccctcctgat
     3721 gagagtggcc ccggctgcat gagctgcaag tgtgtgctct cctgattatc gataatcaac
     3781 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     3841 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     3901 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     3961 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     4021 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     4081 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     4141 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     4201 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     4261 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     4321 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc
     4381 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     4441 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcgtaccttt
     4501 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     4561 actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca
     4621 cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc
     4681 actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc
     4741 caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     4801 ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg
     4861 agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct
     4921 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     4981 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     5041 gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     5101 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     5161 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     5221 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     5281 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     5341 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     5401 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     5461 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     5521 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     5581 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5641 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     5701 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     5761 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     5821 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5881 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5941 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     6001 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     6061 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     6121 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     6181 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     6241 aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc
     6301 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
     6361 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
     6421 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
     6481 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
     6541 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
     6601 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
     6661 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     6721 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     6781 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     6841 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     6901 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     6961 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     7021 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7081 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7141 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7201 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7261 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7321 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7381 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7441 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7501 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7561 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7621 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     7681 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     7741 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     7801 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     7861 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     7921 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     7981 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     8041 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     8101 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8161 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     8221 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     8281 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     8341 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     8401 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     8461 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     8521 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     8581 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     8641 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     8701 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     8761 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     8821 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     8881 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     8941 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     9001 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     9061 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc
     9121 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
     9181 tggaggtcgc tgagtagtgc gcgag
//
