LOCUS       sequence-393454-        9614 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mini-Agrin in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9614)
  TITLE     Jeff Miner Lab Plasmids
REFERENCE   2  (bases 1 to 9614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCI_mini-mAgrin-mSA-Myc-His
FEATURES             Location/Qualifiers
     source          1..9614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    31..314
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     enhancer        505..884
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        885..1088
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1039..1059
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          1224..1356
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1339..1358
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1400..1419
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1400..1418
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1519..1528
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4414..4755
                     /codon_start=1
                     /product="monomeric streptavidin/rhizavidin hybrid (Lim et 
                     al., 2013)"
                     /label=mSA
                     /translation="AEAGITGTWYNQSGSTFTVTAGADGNLTGQYENRAQGTGCQNSPY
                     TLTGRYNGTKLEWRVEWNNSTENCHSRTEWRGQYQGGAEARINTQWNLTYEGGSGPATE
                     QGQDTFTKVK"
     CDS             4756..4776
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             4801..4830
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4840..4869
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4879..4908
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4918..4947
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4957..4986
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5014..5031
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    5068..5656
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5121..5141)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5539..5550)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5744..5762)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    5799..5920
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5810..5829)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5864..5883
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        6165..6522
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6165..6356
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6188..6208)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6373..6508
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6553..7590
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             7569..7577
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    7614..7662
                     /note="synthetic polyadenylation signal"
     promoter        7818..7922
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7923..7991,7992..8783)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8141..8160)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(8941..9529)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9021..9040)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aaaggatctt cacctagatc cttttatagt ccggaaatac aggaacgcac gctggatggc
       61 ccttcgctgg gatggtgaaa ccatgaaaaa tggcagcttc agtggattaa gtgggggtaa
      121 tgtggcctgt accctctggt tgcataggta ttcatacggt taaaatttat caggcgcgat
      181 tgcggcagtt tttcgggtgg tttgttgcca tttttacctg tctgctgccg tgatcgcgct
      241 gaacgcgttt tagcggtgcg tacaattaag ggattatggt aaatccactt actgtctgcc
      301 ctcgtagcca tcgagataaa ccgcagtact ccggccacga tgcgtccggc gtagaggatc
      361 gagatcttca atattggcca ttagccatat tattcattgg ttatatagca taaatcaata
      421 ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct
      481 catgtccaat atgaccgcca tgttggcatt gattattgac tagttattaa tagtaatcaa
      541 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      601 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      661 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      721 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtccgccc cctattgacg
      781 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc
      841 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      901 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      961 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     1021 ataaccccgc cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     1081 gcagagctgg tttagtgaac cgtcagatca ctagaagctt tattgcggta gtttatcaca
     1141 gttaaattgc taacgcagtc agtgcttctg acacaacagt ctcgaactta agctgcagaa
     1201 gttggtcgtg aggcactggg caggtaagta tcaaggttac aagacaggtt taaggagacc
     1261 aatagaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag gcacctattg
     1321 gtcttactga catccacttt gcctttctct ccacaggtgt ccactcccag ttcaattaca
     1381 gctcttaagg ctagagtatt aatacgactc actatagggc tagcaaagcg atcgccgaac
     1441 tcacctatct ccccaacacc taataacatt caatcactct ttccactaac cacctatcta
     1501 catcaccacc caccagccgc caccatggtc cgcccgcggc tgagcttccc ggccccgctg
     1561 ctgccgctgc tgctgctcct ggcggcggcc gcgccagccg tgccgggcgc cagcgggacg
     1621 tgcccggagc gcgccttgga gcggcgcgag gaggaggcga acgtggtgct caccggcaca
     1681 gtggaggaga tcctcaacgt ggacccggtg cagcacacct actcctgcaa ggttcgagtg
     1741 tggcggtact tgaaaggcaa agatgtggtg gcccaagaga gcctgctgga tggtggcaac
     1801 aaggtggtaa ttggtggctt tggagacccc ctcatctgcg acaaccaggt gtccaccggg
     1861 gacactagaa tcttctttgt gaacccagca ccaccctacc tgtggccagc ccacaagaat
     1921 gagttgatgc tcaactccag ccttatgcgg atcactttgc ggaacctgga agaggtggag
     1981 ttctgtgtag aagacaaacc tgggatccac ttcacagcag cgccttctat gcctcctgat
     2041 gtgtgcaggg gaatgttatg tggctttggt gctgtgtgtg aacctagtgt tgaggatcca
     2101 ggccgggcct cctgtgtgtg caagaagaat gtctgccctg ctatggtagc tcctgtgtgt
     2161 ggctcagatg cttccaccta tagcaacgag tgtgagctac agcgtgcaca gtgcaaccag
     2221 caacggcgca tccgcctact ccgccaaggg ccatgtggct ctcccccaaa gtcctgtgat
     2281 tcccagcctt gcctccacgg aggtacctgc caggacctgg attctggcaa gggtttcagc
     2341 tgcagctgta ctgcaggcag ggctggcact gtctgtgaga aagtgcaact cccctctgtg
     2401 ccagctttta agggccactc cttcttggcc ttccccaccc tccgagccta ccacacgctg
     2461 cgtctggcac tagaattccg ggcgctggag acagagggac tgctgctcta caatggcaat
     2521 gcacgtggca aagatttcct ggctctggct ctgttggatg gtcatgtaca gttcaggttc
     2581 gacacgggct cagggccggc ggtgctaaca agcttagtgc cagtggaacc gggacggtgg
     2641 caccgcctcg agttgtcacg gcattggcgg cagggcacac tttctgtgga tggcgaggct
     2701 cctgttgtag gtgaaagtcc cagtgggact gatggcctca acttggacac gaagctctat
     2761 gtgggtggtc tcccagaaga acaagttgcc acggtgcttg atcggacctc tgtgggcatc
     2821 ggcctgaaag gatgcattcg tatgttggac atcaacaacc agcagctgga gctgagcgat
     2881 tggcagaggg ctgtggttca aagctctggt gtgggggaat gcggagacca tccctgctca
     2941 cctaacccct gccatggcgg ggccctctgc caggccctgg aggctggcgt gttcctctgt
     3001 cagtgtccac ctggccgctt tggcccaact tgtgcagatg aaaagaaccc ctgccaaccg
     3061 aacccctgcc acgggtcagc cccctgccat gtgctttcca ggggtggggc caagtgtgtg
     3121 tgccccctgg gacgcagtgg ttccttctgt gagacagtcc tggagaatgc tggctcccgg
     3181 cccttcctgg ctgactttaa tggcttctcc tacctggaac tgaaaggctt gcacaccttc
     3241 gagagagacc taggggagaa gatggcgctg gagatggtgt tcttggctcg tgggcccagt
     3301 ggcttactcc tctacaatgg gcagaagacg gatggcaagg gggactttgt atccctggcc
     3361 ctgcataacc ggcacctaga gttccgctat gaccttggca agggggctgc aatcatcagg
     3421 agcaaagagc ccatagccct gggcacctgg gttagggtat tcctggaacg aaatggccgc
     3481 aagggtgccc ttcaagtggg tgatgggccc cgtgtgctag gggaatctcc ggtcccgcac
     3541 accatgctca acctcaagga gcccctctat gtggggggag ctcctgactt cagcaagctg
     3601 gctcggggcg ctgcagtggc ctccggcttt gatggtgcca tccagctggt gtctctaaga
     3661 ggccatcagc tgctgactca ggagcatgtg ttgcgggcag tagatgtagc gccttttgca
     3721 ggccaccctt gtacccaggc cgtggacaac ccctgcctta atgggggctc ctgtatcccg
     3781 agggaagcca cttatgagtg cctgtgtcct gggggcttct ctgggctgca ctgcgagaag
     3841 gggatagttg agaagtcagt gggggaccta gaaacactgg cctttgatgg gcggacctac
     3901 atcgagtacc tcaatgctgt gactgagagt gagaaagcgc tgcagagcaa ccactttgag
     3961 ctgagcttac gcactgaggc cacgcagggg ctggtgctgt ggattggaaa ggttggagaa
     4021 cgtgcagact acatggctct ggccattgtg gatgggcacc tacaactgag ctatgaccta
     4081 ggctcccagc cagttgtgct gcgctccact gtgaaggtca acaccaaccg ctggcttcga
     4141 gtcagggctc acagggagca cagggaaggt tcccttcagg tgggcaatga agcccctgtg
     4201 actggctctt ccccgctggg tgccacacaa ttggacacag atggagccct gtggcttgga
     4261 ggcctacaga agcttcctgt ggggcaggct ctccccaagg cctatggcac gggttttgtg
     4321 ggctgtctgc gggacgtggt agtgggccat cgccagctgc atctgctgga ggacgctgtc
     4381 accaaaccag agctaagacc ctgccccact ctcgcggaag cgggtatcac cggcacgtgg
     4441 tacaaccagt ctggttctac cttcaccgtt accgcgggtg cggacggtaa cctgaccggt
     4501 cagtacgaaa accgtgcgca gggcactggt tgccagaact ctccgtacac cctgaccggt
     4561 cgttacaacg gtaccaaact ggaatggcgt gttgaatgga acaactctac cgaaaactgc
     4621 cactctcgta ccgaatggcg tggtcagtac cagggtggtg cggaagcgcg tatcaacacc
     4681 cagtggaacc tgacctacga aggtggttct ggtccggcga ccgaacaggg tcaggacacc
     4741 ttcaccaaag ttaaagagga tctgtacttt cagagcatcg atggaagtgg taaagctatg
     4801 gagcaaaagc tcatttctga agaggacttg aatgaaatgg agcaaaagct catttctgaa
     4861 gaggacttga atgaaatgga gcaaaagctc atttctgaag aggacttgaa tgaaatggag
     4921 caaaagctca tttctgaaga ggacttgaat gaaatggagc aaaagctcat ttctgaagag
     4981 gacttgaatg aaatggagag cttgggcgac ctccatcatc accatcacca ctaggggata
     5041 tcagctgaat tctagaaatt catcgacaat caacctctgg attacaaaat ttgtgaaaga
     5101 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     5161 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     5221 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     5281 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     5341 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     5401 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     5461 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     5521 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     5581 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     5641 tgggccgcct ccccgcccag gttccttggg ccgcgactct agggcatgca agctgatccg
     5701 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
     5761 gcataacccc ttggggcggc cgcttcgagc agacatgata agatacattg atgagtttgg
     5821 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     5881 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     5941 ttttatgttt caggttcagg gggagatgtg ggaggttttt ttaagcaagt aaaacctcta
     6001 caaatgtggt aaaatcgaat tttaacaaaa tattaacgct tacaatttcc tgatgcggta
     6061 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgcggatctg cgcagcacca
     6121 tggcctgaaa taacctctga aagaggaact tggttaggta ccttctgagg cggaaagaac
     6181 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     6241 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     6301 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     6361 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     6421 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     6481 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctcga ttcttctgac
     6541 actagcgcca ccatgaagaa gcccgaactc accgctacca gcgttgaaaa atttctcatc
     6601 gagaagttcg acagtgtgag cgacctgatg cagttgtcgg agggcgaaga gagccgagcc
     6661 ttcagcttcg atgtcggcgg acgcggctat gtactgcggg tgaatagctg cgctgatggc
     6721 ttctacaaag accgctacgt gtaccgccac ttcgccagcg ctgcactacc catccccgaa
     6781 gtgttggaca tcggcgagtt cagcgagagc ctgacatact gcatcagtag acgcgcccaa
     6841 ggcgttactc tccaagacct ccccgaaaca gagctgcctg ctgtgttaca gcctgtcgcc
     6901 gaagctatgg atgctattgc cgccgccgac ctcagtcaaa ccagcggctt cggcccattc
     6961 gggccccaag gcatcggcca gtacacaacc tggcgggatt tcatttgcgc cattgctgat
     7021 ccccatgtct accactggca gaccgtgatg gacgacaccg tgtccgccag cgtagctcaa
     7081 gccctggacg aactgatgct gtgggccgaa gactgtcccg aggtgcgcca cctcgtccat
     7141 gccgacttcg gcagcaacaa cgtcctgacc gacaacggcc gcatcaccgc cgtaatcgac
     7201 tggtccgaag ctatgttcgg ggacagtcag tacgaggtgg ccaacatctt cttctggcgg
     7261 ccctggctgg cttgcatgga gcagcagact cgctacttcg agcgccggca tcccgagctg
     7321 gccggcagcc ctcgtctgcg agcctacatg ctgcgcatcg gcctggatca gctctaccag
     7381 agcctcgtgg acggcaactt cgacgatgct gcctgggctc aaggccgctg cgatgccatc
     7441 gtccgcagcg gggccggcac cgtcggtcgc acacaaatcg ctcgccggag cgcagccgta
     7501 tggaccgacg gctgcgtcga ggtgctggcc gacagcggca accgccggcc cagtacacga
     7561 ccgcgcgcta aggaggtagg tcgagtttag actctagcca tcacgatggc cgcaataaaa
     7621 tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaatcgata gcgataagga
     7681 tcctctttgc gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg
     7741 gtcagcaccg tttctgcgga ctggctttct acgtaatggt ttcttagacg tcaggtggca
     7801 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     7861 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     7921 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7981 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     8041 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     8101 ccgaagaacg ttttccaatg atgagcactt tcaaagttct gctatgtggc gcggtattat
     8161 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     8221 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     8281 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacta
     8341 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     8401 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     8461 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     8521 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     8581 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     8641 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     8701 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     8761 cctcactgat taagcattgg taattcgaaa tgaccgacca agcgacgccc aaccggtatc
     8821 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     8881 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8941 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     9001 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     9061 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     9121 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     9181 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     9241 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     9301 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9361 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     9421 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     9481 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatttcaag aagatccttt
     9541 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     9601 catgagatta tcaa
//
