LOCUS       sequence-393456-        9272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mini-Agrin in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9272)
  TITLE     Jeff Miner Lab Plasmids
REFERENCE   2  (bases 1 to 9272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCI_E01_mini-mAgrin-Myc-His-WPRE_v2
FEATURES             Location/Qualifiers
     source          1..9272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    27..310
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     enhancer        501..880
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        881..1084
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1035..1055
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          1220..1352
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1335..1354
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1396..1415
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1396..1414
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1515..1524
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4410..4430
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             4455..4484
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4494..4523
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4533..4562
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4572..4601
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4611..4640
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4668..4685
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    4722..5310
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4775..4795)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5193..5204)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5398..5416)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    5453..5574
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5464..5483)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5518..5537
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5819..6176
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5819..6010
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5842..5862)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6027..6162
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6207..7244
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             7223..7231
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    7268..7316
                     /note="synthetic polyadenylation signal"
     promoter        7472..7576
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7577..7645,7646..8437)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7795..7814)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(8595..9183)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8675..8694)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gatcttcacc tagatccttt tatagtccgg aaatacagga acgcacgctg gatggccctt
       61 cgctgggatg gtgaaaccat gaaaaatggc agcttcagtg gattaagtgg gggtaatgtg
      121 gcctgtaccc tctggttgca taggtattca tacggttaaa atttatcagg cgcgattgcg
      181 gcagtttttc gggtggtttg ttgccatttt tacctgtctg ctgccgtgat cgcgctgaac
      241 gcgttttagc ggtgcgtaca attaagggat tatggtaaat ccacttactg tctgccctcg
      301 tagccatcga gataaaccgc agtactccgg ccacgatgcg tccggcgtag aggatcgaga
      361 tcttcaatat tggccattag ccatattatt cattggttat atagcataaa tcaatattgg
      421 ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg
      481 tccaatatga ccgccatgtt ggcattgatt attgactagt tattaatagt aatcaattac
      541 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
      601 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      661 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      721 tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa
      781 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg actttcctac
      841 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
      901 caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
      961 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa
     1021 ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     1081 agctggttta gtgaaccgtc agatcactag aagctttatt gcggtagttt atcacagtta
     1141 aattgctaac gcagtcagtg cttctgacac aacagtctcg aacttaagct gcagaagttg
     1201 gtcgtgaggc actgggcagg taagtatcaa ggttacaaga caggtttaag gagaccaata
     1261 gaaactgggc ttgtcgagac agagaagact cttgcgtttc tgataggcac ctattggtct
     1321 tactgacatc cactttgcct ttctctccac aggtgtccac tcccagttca attacagctc
     1381 ttaaggctag agtattaata cgactcacta tagggctagc aaagcgatcg ccgaactcac
     1441 ctatctcccc aacacctaat aacattcaat cactctttcc actaaccacc tatctacatc
     1501 accacccacc agccgccacc atggtccgcc cgcggctgag cttcccggcc ccgctgctgc
     1561 cgctgctgct gctcctggcg gcggccgcgc cagccgtgcc gggcgccagc gggacgtgcc
     1621 cggagcgcgc cttggagcgg cgcgaggagg aggcgaacgt ggtgctcacc ggcacagtgg
     1681 aggagatcct caacgtggac ccggtgcagc acacctactc ctgcaaggtt cgagtgtggc
     1741 ggtacttgaa aggcaaagat gtggtggccc aagagagcct gctggatggt ggcaacaagg
     1801 tggtaattgg tggctttgga gaccccctca tctgcgacaa ccaggtgtcc accggggaca
     1861 ctagaatctt ctttgtgaac ccagcaccac cctacctgtg gccagcccac aagaatgagt
     1921 tgatgctcaa ctccagcctt atgcggatca ctttgcggaa cctggaagag gtggagttct
     1981 gtgtagaaga caaacctggg atccacttca cagcagcgcc ttctatgcct cctgatgtgt
     2041 gcaggggaat gttatgtggc tttggtgctg tgtgtgaacc tagtgttgag gatccaggcc
     2101 gggcctcctg tgtgtgcaag aagaatgtct gccctgctat ggtagctcct gtgtgtggct
     2161 cagatgcttc cacctatagc aacgagtgtg agctacagcg tgcacagtgc aaccagcaac
     2221 ggcgcatccg cctactccgc caagggccat gtggctctcc cccaaagtcc tgtgattccc
     2281 agccttgcct ccacggaggt acctgccagg acctggattc tggcaagggt ttcagctgca
     2341 gctgtactgc aggcagggct ggcactgtct gtgagaaagt gcaactcccc tctgtgccag
     2401 cttttaaggg ccactccttc ttggccttcc ccaccctccg agcctaccac acgctgcgtc
     2461 tggcactaga attccgggcg ctggagacag agggactgct gctctacaat ggcaatgcac
     2521 gtggcaaaga tttcctggct ctggctctgt tggatggtca tgtacagttc aggttcgaca
     2581 cgggctcagg gccggcggtg ctaacaagct tagtgccagt ggaaccggga cggtggcacc
     2641 gcctcgagtt gtcacggcat tggcggcagg gcacactttc tgtggatggc gaggctcctg
     2701 ttgtaggtga aagtcccagt gggactgatg gcctcaactt ggacacgaag ctctatgtgg
     2761 gtggtctccc agaagaacaa gttgccacgg tgcttgatcg gacctctgtg ggcatcggcc
     2821 tgaaaggatg cattcgtatg ttggacatca acaaccagca gctggagctg agcgattggc
     2881 agagggctgt ggttcaaagc tctggtgtgg gggaatgcgg agaccatccc tgctcaccta
     2941 acccctgcca tggcggggcc ctctgccagg ccctggaggc tggcgtgttc ctctgtcagt
     3001 gtccacctgg ccgctttggc ccaacttgtg cagatgaaaa gaacccctgc caaccgaacc
     3061 cctgccacgg gtcagccccc tgccatgtgc tttccagggg tggggccaag tgtgtgtgcc
     3121 ccctgggacg cagtggttcc ttctgtgaga cagtcctgga gaatgctggc tcccggccct
     3181 tcctggctga ctttaatggc ttctcctacc tggaactgaa aggcttgcac accttcgaga
     3241 gagacctagg ggagaagatg gcgctggaga tggtgttctt ggctcgtggg cccagtggct
     3301 tactcctcta caatgggcag aagacggatg gcaaggggga ctttgtatcc ctggccctgc
     3361 ataaccggca cctagagttc cgctatgacc ttggcaaggg ggctgcaatc atcaggagca
     3421 aagagcccat agccctgggc acctgggtta gggtattcct ggaacgaaat ggccgcaagg
     3481 gtgcccttca agtgggtgat gggccccgtg tgctagggga atctccggtc ccgcacacca
     3541 tgctcaacct caaggagccc ctctatgtgg ggggagctcc tgacttcagc aagctggctc
     3601 ggggcgctgc agtggcctcc ggctttgatg gtgccatcca gctggtgtct ctaagaggcc
     3661 atcagctgct gactcaggag catgtgttgc gggcagtaga tgtagcgcct tttgcaggcc
     3721 acccttgtac ccaggccgtg gacaacccct gccttaatgg gggctcctgt atcccgaggg
     3781 aagccactta tgagtgcctg tgtcctgggg gcttctctgg gctgcactgc gagaagggga
     3841 tagttgagaa gtcagtgggg gacctagaaa cactggcctt tgatgggcgg acctacatcg
     3901 agtacctcaa tgctgtgact gagagtgaga aagcgctgca gagcaaccac tttgagctga
     3961 gcttacgcac tgaggccacg caggggctgg tgctgtggat tggaaaggtt ggagaacgtg
     4021 cagactacat ggctctggcc attgtggatg ggcacctaca actgagctat gacctaggct
     4081 cccagccagt tgtgctgcgc tccactgtga aggtcaacac caaccgctgg cttcgagtca
     4141 gggctcacag ggagcacagg gaaggttccc ttcaggtggg caatgaagcc cctgtgactg
     4201 gctcttcccc gctgggtgcc acacaattgg acacagatgg agccctgtgg cttggaggcc
     4261 tacagaagct tcctgtgggg caggctctcc ccaaggccta tggcacgggt tttgtgggct
     4321 gtctgcggga cgtggtagtg ggccatcgcc agctgcatct gctggaggac gctgtcacca
     4381 aaccagagct aagaccctgc cccactctcg aggatctgta ctttcagagc atcgatggaa
     4441 gtggtaaagc tatggagcaa aagctcattt ctgaagagga cttgaatgaa atggagcaaa
     4501 agctcatttc tgaagaggac ttgaatgaaa tggagcaaaa gctcatttct gaagaggact
     4561 tgaatgaaat ggagcaaaag ctcatttctg aagaggactt gaatgaaatg gagcaaaagc
     4621 tcatttctga agaggacttg aatgaaatgg agagcttggg cgacctccat catcaccatc
     4681 accactaggg gatatcagct gaattctaga aattcatcga caatcaacct ctggattaca
     4741 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     4801 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     4861 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     4921 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     4981 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     5041 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     5101 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     5161 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     5221 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     5281 gtcggatctc cctttgggcc gcctccccgc ccaggttcct tgggccgcga ctctagggca
     5341 tgcaagctga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
     5401 ctgagcaata actagcataa ccccttgggg cggccgcttc gagcagacat gataagatac
     5461 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     5521 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     5581 aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt ttttttaagc
     5641 aagtaaaacc tctacaaatg tggtaaaatc gaattttaac aaaatattaa cgcttacaat
     5701 ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgcgga
     5761 tctgcgcagc accatggcct gaaataacct ctgaaagagg aacttggtta ggtaccttct
     5821 gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     5881 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     5941 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     6001 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     6061 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     6121 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     6181 tcgattcttc tgacactagc gccaccatga agaagcccga actcaccgct accagcgttg
     6241 aaaaatttct catcgagaag ttcgacagtg tgagcgacct gatgcagttg tcggagggcg
     6301 aagagagccg agccttcagc ttcgatgtcg gcggacgcgg ctatgtactg cgggtgaata
     6361 gctgcgctga tggcttctac aaagaccgct acgtgtaccg ccacttcgcc agcgctgcac
     6421 tacccatccc cgaagtgttg gacatcggcg agttcagcga gagcctgaca tactgcatca
     6481 gtagacgcgc ccaaggcgtt actctccaag acctccccga aacagagctg cctgctgtgt
     6541 tacagcctgt cgccgaagct atggatgcta ttgccgccgc cgacctcagt caaaccagcg
     6601 gcttcggccc attcgggccc caaggcatcg gccagtacac aacctggcgg gatttcattt
     6661 gcgccattgc tgatccccat gtctaccact ggcagaccgt gatggacgac accgtgtccg
     6721 ccagcgtagc tcaagccctg gacgaactga tgctgtgggc cgaagactgt cccgaggtgc
     6781 gccacctcgt ccatgccgac ttcggcagca acaacgtcct gaccgacaac ggccgcatca
     6841 ccgccgtaat cgactggtcc gaagctatgt tcggggacag tcagtacgag gtggccaaca
     6901 tcttcttctg gcggccctgg ctggcttgca tggagcagca gactcgctac ttcgagcgcc
     6961 ggcatcccga gctggccggc agccctcgtc tgcgagccta catgctgcgc atcggcctgg
     7021 atcagctcta ccagagcctc gtggacggca acttcgacga tgctgcctgg gctcaaggcc
     7081 gctgcgatgc catcgtccgc agcggggccg gcaccgtcgg tcgcacacaa atcgctcgcc
     7141 ggagcgcagc cgtatggacc gacggctgcg tcgaggtgct ggccgacagc ggcaaccgcc
     7201 ggcccagtac acgaccgcgc gctaaggagg taggtcgagt ttagactcta gccatcacga
     7261 tggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt tgtgtgaatc
     7321 gatagcgata aggatcctct ttgcgcttgc gttttccctt gtccagatag cccagtagct
     7381 gacattcatc cggggtcagc accgtttctg cggactggct ttctacgtaa tggtttctta
     7441 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     7501 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     7561 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     7621 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     7681 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     7741 tgagagtttt cgccccgaag aacgttttcc aatgatgagc actttcaaag ttctgctatg
     7801 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     7861 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     7921 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     7981 acttctgaca actatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     8041 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     8101 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     8161 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     8221 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     8281 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     8341 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     8401 cgctgagata ggtgcctcac tgattaagca ttggtaattc gaaatgaccg accaagcgac
     8461 gcccaaccgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     8521 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     8581 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     8641 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     8701 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     8761 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     8821 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     8881 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     8941 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9001 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     9061 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     9121 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggattt
     9181 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     9241 taagggattt tggtcatgag attatcaaaa ag
//
