LOCUS       sequence-393463-        9356 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mini-Agrin in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9356)
  TITLE     Jeff Miner Lab Plasmids
REFERENCE   2  (bases 1 to 9356)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCI_mini-mAgrin-AviTag
FEATURES             Location/Qualifiers
     source          1..9356
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    23..306
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     enhancer        497..876
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        877..1080
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1031..1051
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          1216..1348
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1331..1350
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1392..1411
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1392..1410
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1511..1520
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4445..4489
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             4490..4510
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="EDLYFQS"
     CDS             4535..4564
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4574..4603
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4613..4642
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4652..4681
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4691..4720
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4748..4765
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    4802..5390
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4855..4875)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5273..5284)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5478..5496)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    5533..5654
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5544..5563)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5598..5617
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        5899..6256
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5899..6090
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5922..5942)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6107..6242
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6287..7324
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             7303..7311
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    7348..7396
                     /note="synthetic polyadenylation signal"
     promoter        7552..7656
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7657..7725,7726..8517)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7875..7894)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(8675..9263)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8755..8774)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttcacctaga tccttttata gtccggaaat acaggaacgc acgctggatg gcccttcgct
       61 gggatggtga aaccatgaaa aatggcagct tcagtggatt aagtgggggt aatgtggcct
      121 gtaccctctg gttgcatagg tattcatacg gttaaaattt atcaggcgcg attgcggcag
      181 tttttcgggt ggtttgttgc catttttacc tgtctgctgc cgtgatcgcg ctgaacgcgt
      241 tttagcggtg cgtacaatta agggattatg gtaaatccac ttactgtctg ccctcgtagc
      301 catcgagata aaccgcagta ctccggccac gatgcgtccg gcgtagagga tcgagatctt
      361 caatattggc cattagccat attattcatt ggttatatag cataaatcaa tattggctat
      421 tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca
      481 atatgaccgc catgttggca ttgattattg actagttatt aatagtaatc aattacgggg
      541 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      601 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      661 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      721 cacttggcag tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac
      781 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg
      841 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc
      901 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      961 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc
     1021 gccccgttga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
     1081 ggtttagtga accgtcagat cactagaagc tttattgcgg tagtttatca cagttaaatt
     1141 gctaacgcag tcagtgcttc tgacacaaca gtctcgaact taagctgcag aagttggtcg
     1201 tgaggcactg ggcaggtaag tatcaaggtt acaagacagg tttaaggaga ccaatagaaa
     1261 ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat tggtcttact
     1321 gacatccact ttgcctttct ctccacaggt gtccactccc agttcaatta cagctcttaa
     1381 ggctagagta ttaatacgac tcactatagg gctagcaaag cgatcgccga actcacctat
     1441 ctccccaaca cctaataaca ttcaatcact ctttccacta accacctatc tacatcacca
     1501 cccaccagcc gccaccatgg tccgcccgcg gctgagcttc ccggccccgc tgctgccgct
     1561 gctgctgctc ctggcggcgg ccgcgccagc cgtgccgggc gccagcggga cgtgcccgga
     1621 gcgcgccttg gagcggcgcg aggaggaggc gaacgtggtg ctcaccggca cagtggagga
     1681 gatcctcaac gtggacccgg tgcagcacac ctactcctgc aaggttcgag tgtggcggta
     1741 cttgaaaggc aaagatgtgg tggcccaaga gagcctgctg gatggtggca acaaggtggt
     1801 aattggtggc tttggagacc ccctcatctg cgacaaccag gtgtccaccg gggacactag
     1861 aatcttcttt gtgaacccag caccacccta cctgtggcca gcccacaaga atgagttgat
     1921 gctcaactcc agccttatgc ggatcacttt gcggaacctg gaagaggtgg agttctgtgt
     1981 agaagacaaa cctgggatcc acttcacagc agcgccttct atgcctcctg atgtgtgcag
     2041 gggaatgtta tgtggctttg gtgctgtgtg tgaacctagt gttgaggatc caggccgggc
     2101 ctcctgtgtg tgcaagaaga atgtctgccc tgctatggta gctcctgtgt gtggctcaga
     2161 tgcttccacc tatagcaacg agtgtgagct acagcgtgca cagtgcaacc agcaacggcg
     2221 catccgccta ctccgccaag ggccatgtgg ctctccccca aagtcctgtg attcccagcc
     2281 ttgcctccac ggaggtacct gccaggacct ggattctggc aagggtttca gctgcagctg
     2341 tactgcaggc agggctggca ctgtctgtga gaaagtgcaa ctcccctctg tgccagcttt
     2401 taagggccac tccttcttgg ccttccccac cctccgagcc taccacacgc tgcgtctggc
     2461 actagaattc cgggcgctgg agacagaggg actgctgctc tacaatggca atgcacgtgg
     2521 caaagatttc ctggctctgg ctctgttgga tggtcatgta cagttcaggt tcgacacggg
     2581 ctcagggccg gcggtgctaa caagcttagt gccagtggaa ccgggacggt ggcaccgcct
     2641 cgagttgtca cggcattggc ggcagggcac actttctgtg gatggcgagg ctcctgttgt
     2701 aggtgaaagt cccagtggga ctgatggcct caacttggac acgaagctct atgtgggtgg
     2761 tctcccagaa gaacaagttg ccacggtgct tgatcggacc tctgtgggca tcggcctgaa
     2821 aggatgcatt cgtatgttgg acatcaacaa ccagcagctg gagctgagcg attggcagag
     2881 ggctgtggtt caaagctctg gtgtggggga atgcggagac catccctgct cacctaaccc
     2941 ctgccatggc ggggccctct gccaggccct ggaggctggc gtgttcctct gtcagtgtcc
     3001 acctggccgc tttggcccaa cttgtgcaga tgaaaagaac ccctgccaac cgaacccctg
     3061 ccacgggtca gccccctgcc atgtgctttc caggggtggg gccaagtgtg tgtgccccct
     3121 gggacgcagt ggttccttct gtgagacagt cctggagaat gctggctccc ggcccttcct
     3181 ggctgacttt aatggcttct cctacctgga actgaaaggc ttgcacacct tcgagagaga
     3241 cctaggggag aagatggcgc tggagatggt gttcttggct cgtgggccca gtggcttact
     3301 cctctacaat gggcagaaga cggatggcaa gggggacttt gtatccctgg ccctgcataa
     3361 ccggcaccta gagttccgct atgaccttgg caagggggct gcaatcatca ggagcaaaga
     3421 gcccatagcc ctgggcacct gggttagggt attcctggaa cgaaatggcc gcaagggtgc
     3481 ccttcaagtg ggtgatgggc cccgtgtgct aggggaatct ccggtcccgc acaccatgct
     3541 caacctcaag gagcccctct atgtgggggg agctcctgac ttcagcaagc tggctcgggg
     3601 cgctgcagtg gcctccggct ttgatggtgc catccagctg gtgtctctaa gaggccatca
     3661 gctgctgact caggagcatg tgttgcgggc agtagatgta gcgccttttg caggccaccc
     3721 ttgtacccag gccgtggaca acccctgcct taatgggggc tcctgtatcc cgagggaagc
     3781 cacttatgag tgcctgtgtc ctgggggctt ctctgggctg cactgcgaga aggggatagt
     3841 tgagaagtca gtgggggacc tagaaacact ggcctttgat gggcggacct acatcgagta
     3901 cctcaatgct gtgactgaga gtgagaaagc gctgcagagc aaccactttg agctgagctt
     3961 acgcactgag gccacgcagg ggctggtgct gtggattgga aaggttggag aacgtgcaga
     4021 ctacatggct ctggccattg tggatgggca cctacaactg agctatgacc taggctccca
     4081 gccagttgtg ctgcgctcca ctgtgaaggt caacaccaac cgctggcttc gagtcagggc
     4141 tcacagggag cacagggaag gttcccttca ggtgggcaat gaagcccctg tgactggctc
     4201 ttccccgctg ggtgccacac aattggacac agatggagcc ctgtggcttg gaggcctaca
     4261 gaagcttcct gtggggcagg ctctccccaa ggcctatggc acgggttttg tgggctgtct
     4321 gcgggacgtg gtagtgggcc atcgccagct gcatctgctg gaggacgctg tcaccaaacc
     4381 agagctaaga ccctgcccca ctctcagcgc tggaggaggt ggaagcggag gaggaggtag
     4441 cggaggtctg aacgacatct tcgaggctca gaaaatcgaa tggcacgaag aggatctgta
     4501 ctttcagagc atcgatggaa gtggtaaagc tatggagcaa aagctcattt ctgaagagga
     4561 cttgaatgaa atggagcaaa agctcatttc tgaagaggac ttgaatgaaa tggagcaaaa
     4621 gctcatttct gaagaggact tgaatgaaat ggagcaaaag ctcatttctg aagaggactt
     4681 gaatgaaatg gagcaaaagc tcatttctga agaggacttg aatgaaatgg agagcttggg
     4741 cgacctccat catcaccatc accactaggg gatatcagct gaattctaga aattcatcga
     4801 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     4861 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     4921 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     4981 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     5041 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     5101 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     5161 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     5221 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     5281 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     5341 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ccaggttcct
     5401 tgggccgcga ctctagggca tgcaagctga tccggctgct aacaaagccc gaaaggaagc
     5461 tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cggccgcttc
     5521 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     5581 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     5641 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga
     5701 tgtgggaggt ttttttaagc aagtaaaacc tctacaaatg tggtaaaatc gaattttaac
     5761 aaaatattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta
     5821 tttcacaccg catacgcgga tctgcgcagc accatggcct gaaataacct ctgaaagagg
     5881 aacttggtta ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
     5941 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     6001 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6061 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     6121 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     6181 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     6241 cctaggcttt tgcaaaaagc tcgattcttc tgacactagc gccaccatga agaagcccga
     6301 actcaccgct accagcgttg aaaaatttct catcgagaag ttcgacagtg tgagcgacct
     6361 gatgcagttg tcggagggcg aagagagccg agccttcagc ttcgatgtcg gcggacgcgg
     6421 ctatgtactg cgggtgaata gctgcgctga tggcttctac aaagaccgct acgtgtaccg
     6481 ccacttcgcc agcgctgcac tacccatccc cgaagtgttg gacatcggcg agttcagcga
     6541 gagcctgaca tactgcatca gtagacgcgc ccaaggcgtt actctccaag acctccccga
     6601 aacagagctg cctgctgtgt tacagcctgt cgccgaagct atggatgcta ttgccgccgc
     6661 cgacctcagt caaaccagcg gcttcggccc attcgggccc caaggcatcg gccagtacac
     6721 aacctggcgg gatttcattt gcgccattgc tgatccccat gtctaccact ggcagaccgt
     6781 gatggacgac accgtgtccg ccagcgtagc tcaagccctg gacgaactga tgctgtgggc
     6841 cgaagactgt cccgaggtgc gccacctcgt ccatgccgac ttcggcagca acaacgtcct
     6901 gaccgacaac ggccgcatca ccgccgtaat cgactggtcc gaagctatgt tcggggacag
     6961 tcagtacgag gtggccaaca tcttcttctg gcggccctgg ctggcttgca tggagcagca
     7021 gactcgctac ttcgagcgcc ggcatcccga gctggccggc agccctcgtc tgcgagccta
     7081 catgctgcgc atcggcctgg atcagctcta ccagagcctc gtggacggca acttcgacga
     7141 tgctgcctgg gctcaaggcc gctgcgatgc catcgtccgc agcggggccg gcaccgtcgg
     7201 tcgcacacaa atcgctcgcc ggagcgcagc cgtatggacc gacggctgcg tcgaggtgct
     7261 ggccgacagc ggcaaccgcc ggcccagtac acgaccgcgc gctaaggagg taggtcgagt
     7321 ttagactcta gccatcacga tggccgcaat aaaatatctt tattttcatt acatctgtgt
     7381 gttggttttt tgtgtgaatc gatagcgata aggatcctct ttgcgcttgc gttttccctt
     7441 gtccagatag cccagtagct gacattcatc cggggtcagc accgtttctg cggactggct
     7501 ttctacgtaa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     7561 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     7621 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     7681 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     7741 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     7801 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     7861 actttcaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     7921 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     7981 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     8041 gataacactg cggccaactt acttctgaca actatcggag gaccgaagga gctaaccgct
     8101 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     8161 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     8221 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     8281 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     8341 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     8401 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     8461 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaattc
     8521 gaaatgaccg accaagcgac gcccaaccgg tatcagctca ctcaaaggcg gtaatacggt
     8581 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     8641 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     8701 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8761 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     8821 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     8881 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8941 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     9001 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     9061 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     9121 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     9181 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     9241 cgcgcagaaa aaaaggattt caagaagatc ctttgatctt ttctacgggg tctgacgctc
     9301 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatc
//
