LOCUS       sequence-393479-       12299 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of MAP3K14 (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12299)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 12299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-MAP3K14-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..12299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2219..2445
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2446..2626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2673..2798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3291..3524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3709..3753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4020..4136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4168..4188
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4188..5399
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5236..5255
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    5400..5424
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      5427..5436
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(8278..8302)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8304..8345
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             8352..9122
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(9128..9148)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    9177..9765
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9230..9250)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9648..9659)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        9779..10278
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9814..9837)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10211..10230
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             10299..10898
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10299..10318)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10795..10815
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             11036..11269
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11341..11462
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11378..11397)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11432..11451
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11502..11637
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11657..11676)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11658..11676)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11686..11703)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11686..11702)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11695..11717)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11844..12272
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11931..11950)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12141..12162
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggt ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     4141 tccagtgtgg tggaattctg cagatatcaa ctttgtatag aaaagttggc ctccgcgccg
     4201 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga
     4261 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
     4321 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
     4381 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
     4441 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
     4501 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
     4561 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
     4621 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
     4681 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
     4741 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
     4801 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
     4861 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
     4921 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg
     4981 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg
     5041 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
     5101 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
     5161 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
     5221 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
     5281 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
     5341 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagaca
     5401 caagtttgta caaaaaagca ggcttagcca ccatggcagt gatggaaatg gcctgcccag
     5461 gtgcccctgg ctcagcagtg gggcagcaga aggaactccc caaagccaag gagaagacgc
     5521 cgccactggg gaagaaacag agctccgtct acaagcttga ggccgtggag aagagccctg
     5581 tgttctgcgg aaagtgggag atcctgaatg acgtgattac caagggcaca gccaaggaag
     5641 gctccgaggc agggccagct gccatctcta tcatcgccca ggctgagtgt gagaatagcc
     5701 aagagttcag ccccaccttt tcagaacgca ttttcatcgc tgggtccaaa cagtacagcc
     5761 agtccgagag tcttgatcag atccccaaca atgtggccca tgctacagag ggcaaaatgg
     5821 cccgtgtgtg ttggaaggga aagcgtcgca gcaaagcccg gaagaaacgg aagaagaaga
     5881 gctcaaagtc cctggctcat gcaggagtgg ccttggccaa acccctcccc aggacccctg
     5941 agcaggagag ctgcaccatc ccagtgcagg aggatgagtc tccactcggc gccccatatg
     6001 ttagaaacac cccgcagttc accaagcctc tgaaggaacc aggccttggg caactctgtt
     6061 ttaagcagct tggcgagggc ctacggccgg ctctgcctcg atcagaactc cacaaactga
     6121 tcagcccctt gcaatgtctg aaccacgtgt ggaaactgca ccacccccag gacggaggcc
     6181 ccctgcccct gcccacgcac cccttcccct atagcagact gcctcatccc ttcccattcc
     6241 accctctcca gccctggaaa cctcaccctc tggagtcctt cctgggcaaa ctggcctgtg
     6301 tagacagcca gaaacccttg cctgacccac acctgagcaa actggcctgt gtagacagtc
     6361 caaagcccct gcctggccca cacctggagc ccagctgcct gtctcgtggt gcccatgaga
     6421 agttttctgt ggaggaatac ctagtgcatg ctctgcaagg cagcgtgagc tcaggccagg
     6481 cccacagcct gaccagcctg gccaagacct gggcagcaag gggctccaga tcccgggagc
     6541 ccagccccaa aactgaggac aacgagggtg tcctgctcac tgagaaactc aagccagtgg
     6601 attatgagta ccgagaagaa gtccactggg ccacgcacca gctccgcctg ggcagaggct
     6661 ccttcggaga ggtgcacagg atggaggaca agcagactgg cttccagtgc gctgtcaaaa
     6721 aggtgcggct ggaagtattt cgggcagagg agctgatggc atgtgcagga ttgacctcac
     6781 ccagaattgt ccctttgtat ggagctgtga gagaagggcc ttgggtcaac atcttcatgg
     6841 agctgctgga aggtggctcc ctgggccagc tggtcaagga gcagggctgt ctcccagagg
     6901 accgggccct gtactacctg ggccaggccc tggagggtct ggaatacctc cactcacgaa
     6961 ggattctgca tggggacgtc aaagctgaca acgtgctcct gtccagcgat gggagccacg
     7021 cagccctctg tgactttggc catgctgtgt gtcttcaacc tgatggcctg ggaaagtcct
     7081 tgctcacagg ggactacatc cctggcacag agacccacat ggctccggag gtggtgctgg
     7141 gcaggagctg cgacgccaag gtggatgtct ggagcagctg ctgtatgatg ctgcacatgc
     7201 tcaacggctg ccacccctgg actcagttct tccgagggcc gctctgcctc aagattgcca
     7261 gcgagcctcc gcctgtgagg gagatcccac cctcctgcgc ccctctcaca gcccaggcca
     7321 tccaagaggg gctgaggaaa gagcccatcc accgcgtgtc tgcagcggag ctgggaggga
     7381 aggtgaaccg ggcactacag caagtgggag gtctgaagag cccttggagg ggagaatata
     7441 aagaaccaag acatccaccg ccaaatcaag ccaattacca ccagaccctc catgcccagc
     7501 cgagagagct ttcgccaagg gccccagggc cccggccagc tgaggagaca acaggcagag
     7561 cccctaagct ccagcctcct ctcccaccag agcccccaga gccaaacaag tctcctccct
     7621 tgactttgag caaggaggag tctgggatgt gggaaccctt acctctgtcc tccctggagc
     7681 cagcccctgc cagaaacccc agctcaccag agcggaaagc aaccgtcccg gagcaggaac
     7741 tgcagcagct ggaaatagaa ttattcctca acagcctgtc ccagccattt tctctggagg
     7801 agcaggagca aattctctcg tgcctcagca tcgacagcct ctccctgtcg gatgacagtg
     7861 agaagaaccc atcaaaggcc tctcaaagct cgcgggacac cctgagctca ggcgtacact
     7921 cctggagcag ccaggccgag gctcgaagct ccagctggaa catggtgctg gcccgggggc
     7981 ggcccaccga caccccaagc tatttcaatg gtgtgaaagt ccaaatacag tctcttaatg
     8041 gtgaacacct gcacatccgg gagttccacc gggtcaaagt gggagacatc gccactggca
     8101 tcagcagcca gatcccagct gcagccttca gcttggtgac caaagacggg cagcctgttc
     8161 gctacgacat ggaggtgcca gactcgggca tcgacctgca gtgcacactg gcccctgatg
     8221 gcagcttcgc ctggagctgg agggtcaagc atggccagct ggagaacagg ccctcaaacc
     8281 cagctttctt gtacaaagtg ggcggcaagc ccatccccaa ccccctgctg ggcctggaca
     8341 gcaccgctag catcccgctg ctgaacgcta aacagattct gggacagctg gacggcggga
     8401 gcgtggcagt cctgcctgtg gtcgactcca ccaatcagta cctgctggat cgaatcggcg
     8461 agctgaagag tggggatgct tgcattgcag aatatcagca ggcagggaga ggaagcagag
     8521 ggaggaaatg gttctctcct tttggagcta acctgtacct gagtatgttt tggcgcctga
     8581 agcggggacc agcagcaatc ggcctgggcc cggtcatcgg aattgtcatg gcagaagcgc
     8641 tgcgaaagct gggagcagac aaggtgcgag tcaaatggcc caatgacctg tatctgcagg
     8701 atagaaagct ggcaggcatc ctggtggagc tggccggaat aacaggcgat gctgcacaga
     8761 tcgtcattgg cgccgggatt aacgtggcta tgaggcgcgt ggaggaaagc gtggtcaatc
     8821 agggctggat cacactgcag gaagcaggga ttaacctgga caggaatact ctggccgcta
     8881 tgctgatccg agagctgcgg gcagccctgg aactgttcga gcaggaaggc ctggctccat
     8941 atctgtcacg gtgggagaag ctggataact tcatcaatag acccgtgaag ctgatcattg
     9001 gggacaaaga gattttcggg attagccggg ggattgataa acagggagcc ctgctgctgg
     9061 aacaggacgg agttatcaaa ccctggatgg gcggagaaat cagtctgcgg tctgccgaaa
     9121 agtaagccaa ctttattata catagttgat atccagcaca gtggcggccg ctcgacaatc
     9181 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     9241 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     9301 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     9361 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     9421 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     9481 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     9541 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     9601 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     9661 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     9721 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attctaccgg
     9781 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc
     9841 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc
     9901 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg
     9961 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt
    10021 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg
    10081 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt
    10141 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc
    10201 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc
    10261 tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg
    10321 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
    10381 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
    10441 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
    10501 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
    10561 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
    10621 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
    10681 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
    10741 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
    10801 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
    10861 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc
    10921 gcccgaccga aaggagcgca cgaccccatg catctcgagg gcccggtacc tttaagacca
    10981 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
    11041 gggctagctc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
    11101 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
    11161 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
    11221 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
    11281 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
    11341 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    11401 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    11461 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
    11521 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
    11581 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
    11641 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
    11701 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
    11761 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
    11821 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
    11881 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
    11941 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
    12001 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
    12061 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
    12121 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
    12181 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
    12241 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattt
//
