LOCUS       sequence-393501-        7545 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Construct for chemogenetically activating and visualizing 
            subpopulations of neurons in the retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7545)
  TITLE     Intrsectional chemogenetic viruses for retinal neurobiology
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-hSyn-con/fon DREADD Gq-mCherry
FEATURES             Location/Qualifiers
     source          1..7545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        175..622
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     protein_bind    636..669
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(990..1009)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(1272..1305)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(1306..1339)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     regulatory      2058..2067
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2209..2227)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     protein_bind    2309..2342
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    2343..2376
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     protein_bind    complement(3580..3613)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     misc_feature    3672..4260
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3725..3745)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4143..4154)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4292..4768
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4491..4510)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4808..4948
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4808..4937
                     /label=AAV2 ITR
     rep_origin      5023..5478
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5110..5129)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5320..5341
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5495..5514)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5614..5636
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        5760..5864
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5865..5933,5934..6725)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6083..6102)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6896..7484
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7385..7404
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtgtct agactgcaga gggccctgcg tatgagtgca
      181 agtgggtttt aggaccagga tgaggcgggg tgggggtgcc tacctgacga ccgaccccga
      241 cccactggac aagcacccaa cccccattcc ccaaattgcg catcccctat cagagagggg
      301 gaggggaaac aggatgcggc gaggcgcgtg cgcactgcca gcttcagcac cgcggacagt
      361 gccttcgccc ccgcctggcg gcgcgcgcca ccgccgcctc agcactgaag gcgcgctgac
      421 gtcactcgcc ggtcccccgc aaactcccct tcccggccac cttggtcgcg tccgcgccgc
      481 cgccggccca gccggaccgc accacgcgag gcgcgagata ggggggcacg ggcgcgacca
      541 tctgcgctgc ggcgccggcg actcagcgct gcctcagtct gcggtgggca gcggaggagt
      601 cgtgtcgtgc ctgagagcgc agtcgagaag gtaccgaagt tcctattctt caaatagtat
      661 aggaacttcg aagttcctat tcttcaaaag gtataggaac ttcggcgcgc cttacttgta
      721 cagctcgtcc atgccgccgg tggagtggcg gccctcggcg cgttcgtact gttccacgat
      781 ggtgtagtcc tcgttgtggg aggtgatgtc caacttgatg ttgacgttgt aggcgccggg
      841 cagctgcacg ggcttcttgg ccttgtaggt ggtcttgacc tcagcgtcgt agtggccgcc
      901 gtccttcagc ttcagcctct gcttgatctc gcccttcagg gcgccgtcct cggggtacat
      961 ccgctcggag gaggcctccc agcccatggt cttcttctgc attacggggc cgtcggaggg
     1021 gaagttggtg ccgcgcagct tcaccttgta gatgaactcg ccgtcctgca gggaggagtc
     1081 ctgggtcacg gtcaccacgc cgccgtcctc gaagttcatc acgcgctccc acttgaagcc
     1141 ctcggggaag gacagcttca agtagtcggg gatgtcggcg gggtgcttca cgtaggcctg
     1201 tgggacgaca tgacttaacc agttaaccat gttctctgaa catgagaagg gctggccact
     1261 ctccagtcga cataacttcg tataggatac tttatacgaa gttatataac ttcgtatagt
     1321 ataccttata cgaagttata ctagttgtgc cccccctttt ttttataaaa ttgtattaat
     1381 gttatataca tatctcctgt atgtgaccca tgtgcttatg actctatttc tcatgtgttt
     1441 aggtgcctga ggaggagctg gggatggtgg acttggagag gaaagccgac aagctgcagg
     1501 cccagaagag cgtggacgat ggaggcagtt ttccaaaaag cttctccaag cttcccatcc
     1561 agctagagtc agccgtggac acagctaaga cttctgacgt caactcctca gtgggtaaga
     1621 gcacggccac tctacctctg tccttcaagg aagccactct ggccaagagg tttgctctga
     1681 agaccagaag tcagatcact aagcggaaaa ggatgtccct ggtcaaggag aagaaagcgg
     1741 cccagaccct cagtgcgatc ttgcttgcct tcatcatcac ttggacccca tacaacatca
     1801 tggttctggt gaacaccttt tgtgacagct gcatacccaa aaccttttgg aatctgggct
     1861 actggctgtg ctacatcaac agcaccgtga accccgtgtg ctatgctctg tgcaacaaaa
     1921 cattcagaac cactttcaag atgctgctgc tgtgccagtg tgacaaaaaa aagaggcgca
     1981 agcagcagta ccagcagaga cagtcggtca tttttcacaa gcgcgcaccc gagcaggcct
     2041 tgaaggatcc cccggtcgcc accatggtga gcaagggcga ggaggataac atggccatca
     2101 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
     2161 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     2221 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
     2281 gctccaaggt gagtacagga ggggatccat aacttcgtat aaagtatcct atacgaagtt
     2341 atataacttc gtataaggta tactatacga agttatgcta gcaaccccga cacttacctg
     2401 caggttgtcc gatgagggta acttggtgga gttgaggatg gtgctgtgac ccggaagctt
     2461 gagcacgatg gagtagatgg ctctcgtctc ggagccaatg tcctcctcgt cggaggaggc
     2521 ggagttctcc agggaggcag cagcatcatt gttgttccaa ctgtcactgc tgctgtggtc
     2581 ttggtccatc tgctcggagc tgggtttcca gctcttggtt gtgaaccaga agtggcagcg
     2641 gccatacttc ctcctgttgg agcgtttcat gctttgctgt tgaagttcgt aactgctgca
     2701 gcttcgagaa ctgcccgtgg ggtggacaaa gttttctgtc tctgcctctg tcccagaggc
     2761 ttgcaggcca gcaagctctt tggtacgctt ttcagtttcc ttatagatcc tccagtataa
     2821 aatagtcata atggtgacag gcatataaaa accagcgatg gctgtgccaa aagtaatggt
     2881 gggctcactg aggaactgaa tgaagcactc tcccggaggc acagttctct ttccaacaaa
     2941 gtattgccag aacaagatgg caggagccca aaggacaaag gagatgaccc aagccagacc
     3001 gatcatcaca ccggctctct ttgttgttcg tttggctcgg tacgtgagcg gcctcgtgat
     3061 ggaaaagtat ctgtcaaagc tgatgaccag aagattcata acagaggcat tgctggctac
     3121 gcagtcaatg gcaagccaga ggtcacaggc caagttccct aaggcccatc gattcatgat
     3181 gatgtaggtc gtaaacagat tcattgaaat gaccccgata atcagatcgg cacaggccag
     3241 gcttaagagg aagtagttgt tgaccgtctt cagctgcttg ttgaccttaa atgacacaat
     3301 taccaggatg ttgccgatga tggtcaccaa ggccaggatg cccgttaaga aagcgatgaa
     3361 gaccacttgc cagacggtat gacctcccag agggtcatcg gtggttccgt ctggagagga
     3421 gaaattgcca gctgctcgag aaacattgta gctgccgaaa tgagtgacgg ttcccggggg
     3481 cagccctgca tcggaggggc tgtgtatcca ggaggagctg atgtttggaa acaaaggcga
     3541 ggttgtactg ttattgtgca aggtcatggt ggcgagctcg aagttcctat actatttgaa
     3601 gaataggaac ttcgaagttc ctataccttt tgaagaatag gaacttcgaa ttcgatatca
     3661 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     3721 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     3781 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     3841 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     3901 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     3961 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     4021 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     4081 cttggctgct cgcctatgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     4141 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     4201 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     4261 atcgataccg agcgctgctc gagagatcta cgggtggcat ccctgtgacc cctccccagt
     4321 gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt
     4381 aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt
     4441 ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga
     4501 accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca
     4561 agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct
     4621 cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc
     4681 caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc
     4741 gtgaaccact gctcccttcc ctgtccttct gattttgtag gtaaccacgt gcggaccgag
     4801 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     4861 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     4921 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
     4981 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
     5041 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct
     5101 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     5161 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     5221 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     5281 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     5341 gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
     5401 cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     5461 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     5521 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     5581 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     5641 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     5701 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     5761 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5821 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5881 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5941 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     6001 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     6061 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     6121 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     6181 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     6241 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     6301 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     6361 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     6421 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     6481 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     6541 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     6601 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6661 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     6721 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     6781 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6841 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6901 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6961 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7021 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     7081 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     7141 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     7201 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     7261 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     7321 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     7381 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     7441 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     7501 cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//
