LOCUS       sequence-393503-        8536 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BAC targeting cassette for pIndigoBAC-536 with tetR selection marker
            and lens-specific transgenesis marker dsRed.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8536)
  AUTHORS   Fuentes F, Reynolds E, Lewellis SW, Venkiteswaran G, Knaut H
  TITLE     A Plasmid Set for Efficient Bacterial Artificial Chromosome (BAC) 
            Transgenesis in Zebrafish.
  JOURNAL   G3 (Bethesda). 2016 Jan 27. pii: g3.115.026344. doi: 
            10.1534/g3.115.026344.
  PUBMED    26818072
REFERENCE   2  (bases 1 to 8536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2016 Jan 27. pii: g3.115.026344. doi: 
            10.1534/g3.115.026344."
COMMENT     Sequence Label: pBS-IndHom-Tol2-TetR-cryaa-dsRed
FEATURES             Location/Qualifiers
     source          1..8536
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(678..694)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    702..718
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(726..732,733..750,751..756))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    771..792
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    join(1352..1514,1515..1526)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     promoter        join(1827..1832,1833..1849,1850..1855)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             1903..3093
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(2255..2274)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     misc_feature    join(3781..3792,3793..3980)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     regulatory      5019..5028
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5025..5702
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MAPSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPAEIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(5155..5173)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(5205..5225)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     5423..5442
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5617..5640
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    5827..5948
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5864..5883)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5918..5937
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(6348..6366)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6387..6403)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6411..6427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6435..6441,6442..6459,6460..6465))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6480..6501
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6618..6635)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6789..7377)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6869..6888)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7548..8339,8340..8408))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8171..8190
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8409..8513)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 skramkygym mkskttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaawr mkmykmwmsm kkrcaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg agctctaaat
      661 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
      721 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
      781 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
      841 cagctgcatt aatgaatcgg ccaacgcgaa ccccttgcgg ccgcccgggc cgtcgaccaa
      901 ttctcatgtt tgacagctta tcatcgaatt tctgccattc atccgcttat tatcacttat
      961 tcaggcgtag caaccccgcg gtggcggccg catctggcct tgtttcagac accagggagt
     1021 ctctgctcac gtttcctgct atttgcagcc tctctatcaa gactaataca cctcttcccg
     1081 catcggctgc ctgtgagagg cttttcagca ctgcgggatt gcttttcagc cccaaaagag
     1141 ctaggcttga cactaacaat tttgagaatc agcttctact gaagttaaat ctgaggtttt
     1201 acaactttga gtagcgtgta ctggcgttag attgtctgtc ttatagtttg ataattaaat
     1261 acaaacagtt ctaaagcagg ataaaacctt gtatgcattt catttaatgt tttttgagat
     1321 taaaagctta aacaagaatc tctagttttc tttcttgctt ttacttttac ttccttaata
     1381 ctcaagtaca attttaatgg agtacttttt tacttttact caagtaagat tctagccaga
     1441 tacttttact tttaattgag taaaattttc cctaagtact tgtactttca cttgagtaaa
     1501 atttttgagt actttttaca cctctgtcaa gaactcctgg acaaacctct gacctgtgtg
     1561 gaacagagtg gatatgggtg tctgaacaga tattcacgtc ttttgcagat cagagggcat
     1621 ttctggtgtt gtgcaatgac ctgggtccaa caggtaggcg tactcgtatc caagagcatt
     1681 gttggacagg aggtagtctc cattcctata aagaggaagg aatggcacca tgtagtagcc
     1741 gtcattgtgg ccaattggtg cattggtacg tgggtagatg gaccggggag gctgatgagt
     1801 tcttagccag cggatctttc tcatgtttga cagcttatca tcgataagct ttaatgcggt
     1861 agtttatcac agttaaattg ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg
     1921 ctcatcgtca tcctcggcac cgtcaccctg gatgctgtag gcataggctt ggttatgccg
     1981 gtactgccgg gcctcttgcg ggatatcgtc cattccgaca gcatcgccag tcactatggc
     2041 gtgctgctag cgctatatgc gttgatgcaa tttctatgcg cacccgttct cggagcactg
     2101 tccgaccgct ttggccgccg cccagtcctg ctcgcttcgc tacttggagc cactatcgac
     2161 tacgcgatca tggcgaccac acccgtcctg tggatcctct acgccggacg catcgtggcc
     2221 ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg
     2281 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
     2341 ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg
     2401 gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag
     2461 ggagagcgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg
     2521 cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga
     2581 caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg
     2641 atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc
     2701 actggtcccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc
     2761 gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt
     2821 atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg
     2881 caggtagatg acgaccatca gggacagctt caaggatcgc tcgcggctct taccagccta
     2941 acttcgatca ttggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg
     3001 aacgggttgg catggattgt aggcgccgcc ctataccttg tctgcctccc cgcgttgcgt
     3061 cgcggtgcat ggagccgggc cacctcgacc tgaatggaag ccggcggcac ctcgctaacg
     3121 gattcaccac tccaagaatt ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa
     3181 ccaacccttg gcagaacata tccatcgcgt ccgccatctc cagcagccgc acgcggcgca
     3241 tctcgggcag cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga
     3301 cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc
     3361 gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct
     3421 tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt
     3481 tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa
     3541 cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca
     3601 gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg
     3661 tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca
     3721 cggaggcatc agtgaccaga tccgatctgc tgggcttgct gaaggtaggg ggtcaagaac
     3781 cagaggtgta aagtacttga gtaattttac ttgattactg tacttaagta ttatttttgg
     3841 ggatttttac tttacttgag tacaattaaa aatcaatact tttactttta cttaattaca
     3901 tttttttaga aaaaaaagta ctttttactc cttacaattt tatttacagt caaaaagtac
     3961 ttattttttg gagatcactt cattctattt tcccttgcta ttaccaaacc aattgaattg
     4021 cgctgatgcc cagtttaatt taaatgttat ttattctgcc tatgaaaatc gttttcacat
     4081 tatatgaaat tggtcagaca tgttcattgg tcctttggaa gtgacgtcat gtcacatcta
     4141 ttaccacaat gcacagcacc ttgacctgga aattagggaa attatatcag tcaatcagtg
     4201 gaagaaaatg gaggaagtat gtgattcatc agcagctgcg agcagcacag tccaaaatca
     4261 gccacaggat caagagcacc cgtggccgta tcttcgcccc gggagcttcg aattctaata
     4321 tggcaataaa cgatcttcag agaaacttgc attggtaaat agtttcagcc tatgcatatg
     4381 tagttattca aactgtaatg actatcttac agtattatat gtttataata gtttgatcca
     4441 ataaatccag atttatatgc ttttcaacat aaaattgtga aatgcacaga atgtacatat
     4501 acaatacatt tcctacaata atgctctctt ttctattaat agtgtgcatt cagtgcagga
     4561 tgttatgtat ttattttctg tagtgcaaac tttttcaaag aattttccag tctaagcttt
     4621 tcagtcaaga aaaaaaaaaa gcgattgatt atctgtgata tttaaaaaaa ttagctctta
     4681 aatctggtga accaagtggc cacaacacat taaatactga cgttctacag ccatgtagct
     4741 tcatctggtc tggtttgctt tggcaggcac ttattttccg gtgatagttg gccacaagag
     4801 tattgtctga gccattcagt gctagactgt cattctcagg tcagagtcca ccccgctgat
     4861 tctgctgaca acactgttcc caccatgaga taatgccatt ccagagagat ccatttgtaa
     4921 gcccctcttt ctgcagcaca ggtatataac caggggtctg cctccactaa ggccggcaca
     4981 catcatttgg ggatctttgt actgtggatc caccggtcgc caccatggcc ccctccgagg
     5041 acgtcatcaa ggagttcatg cgcttcaagg tgcgcatgga gggctccgtg aacggccacg
     5101 aattcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     5161 tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc ccccagttcc
     5221 agtacggctc caaggtgtac gtgaagcacc ccgccgaaat ccccgactac aagaagctgt
     5281 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     5341 ccgtgaccca ggactcctcc ctgcaggacg gctccttcat ctacaaggtg aagttcatcg
     5401 gcgtgaactt cccctccgac ggccccgtaa tgcagaagaa gactatgggc tgggaggcct
     5461 ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac aaggccctga
     5521 agctgaagga cggcggccac tacctggtgg agttcaagtc catctacatg gccaagaagc
     5581 ccgtgcagct gcccggctac tactacgtgg actccaagct ggacatcacc tcccacaacg
     5641 aggactacac catcgtggag cagtacgagc gcgccgaggg ccgccaccac ctgttcctgt
     5701 agcggccggc cgcgactcta gatcataatc agccatacca tatttgtaga ggttttactt
     5761 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     5821 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     5881 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     5941 gtatcttaat cgaggggggg cccggtaccc aattcgccct atagtgagtc tcgagaaaat
     6001 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
     6061 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
     6121 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
     6181 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc
     6241 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     6301 tatatgtgta gaaacctcga gggggggccc ggtaccagct tttgttccct ttagtgaggg
     6361 ttaatttcga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     6421 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6481 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6541 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     6601 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     6661 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     6721 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6781 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6841 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6901 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     6961 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7021 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7081 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7141 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7201 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     7261 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7321 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7381 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7441 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7501 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7561 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7621 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7681 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     7741 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7801 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7861 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7921 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     7981 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8041 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8101 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8161 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8221 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8281 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8341 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8401 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8461 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8521 cccckraawg kssmay
//
