LOCUS       sequence-393508-       11102 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd gen lentiviral plasmid for expressing endoplasmic reticulum 
            localized TurboID with EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11102)
  AUTHORS   Yan T, Julio AR, Villanueva M, Jones AE, Ball AB, Boatner LM, Turmon
            AC, Nguyen KB, Yen SL, Desai HS, Divakaruni AS, Backus KM
  TITLE     Proximity-labeling chemoproteomics defines the subcellular 
            cysteinome and inflammation-responsive mitochondrial redoxome.
  JOURNAL   Cell Chem Biol. 2023 Jul 20;30(7):811-827.e7. doi: 
            10.1016/j.chembiol.2023.06.008. Epub 2023 Jul 6.
  PUBMED    37419112
REFERENCE   2  (bases 1 to 11102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2023.06.008"; journalName: "Cell Chem Biol"; 
            date: "2023-07-20- 20"; volume: "30"; issue: "7"; pages: 
            "811-827.e7"
COMMENT     Sequence Label: FUGW-ER-TurboID
FEATURES             Location/Qualifiers
     source          1..11102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        173..552
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        554..752
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     707..727
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             770..950
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    997..1122
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1615..1848
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2033..2077
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2375..2492
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2557..3768
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3605..3624
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3808..3817
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3949..4905
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             4912..4953
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      4963..4972
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4969..4971,4972..4974,4975..5688)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4969..4971,4972..4974,4975..5685)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5275..5294)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5622..5643
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5729..6317
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5782..5802)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6200..6211)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6320..6336)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6321..6337)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6842..7022
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7048..7065)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7054..7278
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7324..7752
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7411..7430)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7621..7642
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7761..7781)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7766..8095
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7946..8081
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8008..8027
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8143..8190
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8209..8583
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8713..8834
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8750..8769)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8804..8823
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8883..8899)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8883..8899)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8896..8918)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8907..8923
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8931..8937,8938..8955,8956..8961))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8976..8997
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9114..9131)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9285..9873)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9365..9384)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10044..10835,10836..10904))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10905..11009)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(join(11084..11102,1))
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg
       61 agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt
      121 agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga
      181 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
      241 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      301 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      361 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      421 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      481 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      541 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      601 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      661 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      721 aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg ggtctctctg
      781 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      841 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      901 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      961 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     1021 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     1081 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     1141 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     1201 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     1261 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     1321 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     1381 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     1441 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     1501 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1561 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1621 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1681 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1741 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1801 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1861 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1921 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1981 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     2041 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     2101 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     2161 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     2221 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     2281 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc
     2341 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     2401 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     2461 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     2521 cagtttggtt aattaacccg tgtcggctcc agatctggcc tccgcgccgg gttttggcgc
     2581 ctcccgcggg cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg
     2641 agcgtcctga tccttccgcc cggacgctca ggacagcggc ccgctgctca taagactcgg
     2701 ccttagaacc ccagtatcag cagaaggaca ttttaggacg ggacttgggt gactctaggg
     2761 cactggtttt ctttccagag agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt
     2821 ctgcggaggg atctccgtgg ggcggtgaac gccgatgatt atataaggac gcgccgggtg
     2881 tggcacagct agttccgtcg cagccgggat ttgggtcgcg gttcttgttt gtggatcgct
     2941 gtgatcgtca cttggtgagt agcgggctgc tgggctggcc ggggctttcg tggccgccgg
     3001 gccgctcggt gggacggaag cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg
     3061 agcaaggttg ccctgaactg ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga
     3121 gtcttgaatg gaagacgctt gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc
     3181 atggtgggcg gcaagaaccc aaggtcttga ggccttcgct aatgcgggaa agctcttatt
     3241 cgggtgagat gggctggggc accatctggg gaccctgacg tgaagtttgt cactgactgg
     3301 agaactcggt ttgtcgtctg ttgcgggggc ggcagttatg gcggtgccgt tgggcagtgc
     3361 acccgtacct ttgggagcgc gcgccctcgt cgtgtcgtga cgtcacccgt tctgttggct
     3421 tataatgcag ggtggggcca cctgccggta ggtgtgcggt aggcttttct ccgtcgcagg
     3481 acgcagggtt cgggcctagg gtaggctctc ctgaatcgac aggcgccgga cctctggtga
     3541 ggggagggat aagtgaggcg tcagtttctt tggtcggttt tatgtaccta tcttcttaag
     3601 tagctgaagc tccggttttg aactatgcgc tcggggttgg cgagtgtgtt ttgtgaagtt
     3661 ttttaggcac cttttgaaat gtaatcattt gggtcaatat gtaattttca gtgttagact
     3721 agtaaattgt ccgctaaatt ctggccgttt ttggcttttt tgttagacga agcttgggct
     3781 gcaggtcgac tctagaggat ccgggccgcc accatggacc ctgtggtcgt gctggggctg
     3841 tgcctgagct gtctgctgct gctgagcctg tggaagcagt cctacggagg cgggggatca
     3901 ggaagcggcg gccgcggatc aggaagcgga tcaggaagcg gatcaggaaa agacaatact
     3961 gtgcctctga agctgatcgc tctcctggct aatggcgagt tccatagtgg cgaacagctg
     4021 ggagaaaccc tgggcatgtc cagggccgct atcaacaagc acattcagac tctgcgcgac
     4081 tggggcgtgg acgtgttcac cgtgcccgga aagggctact ctctgcccga gcctatcccg
     4141 ctgctgaacg ctaaacagat tctgggacag ctggacggcg ggagcgtggc agtcctgcct
     4201 gtggtcgact ccaccaatca gtacctgctg gatcgaatcg gcgagctgaa gagtggggat
     4261 gcttgcattg cagaatatca gcaggcaggg agaggaagca gagggaggaa atggttctct
     4321 ccttttggag ctaacctgta cctgagtatg ttttggcgcc tgaagcgggg accagcagca
     4381 atcggcctgg gcccggtcat cggaattgtc atggcagaag cgctgcgaaa gctgggagca
     4441 gacaaggtgc gagtcaaatg gcccaatgac ctgtatctgc aggatagaaa gctggcaggc
     4501 atcctggtgg agctggccgg aataacaggc gatgctgcac agatcgtcat tggcgccggg
     4561 attaacgtgg ctatgaggcg cgtggaggaa agcgtggtca atcagggctg gatcacactg
     4621 caggaagcag ggattaacct ggacaggaat actctggccg ctacgctgat ccgagagctg
     4681 cgggcagccc tggaactgtt cgagcaggaa ggcctggctc catatctgcc acggtgggag
     4741 aagctggata acttcatcaa tagacccgtg aagctgatca ttggggacaa agagattttc
     4801 gggattagcc gggggattga taaacaggga gccctgctgc tggaacagga cggagttatc
     4861 aaaccctgga tgggcggaga aatcagtctg cggtctgccg aaaaggctag cggcaagccc
     4921 atccccaacc ccctgctggg cctggacagc accggaccgg tcgccaccat ggtgagcaag
     4981 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     5041 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     5101 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     5161 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     5221 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     5281 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     5341 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     5401 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     5461 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     5521 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     5581 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     5641 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgac
     5701 tctagaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     5761 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     5821 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     5881 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     5941 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     6001 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     6061 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     6121 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     6181 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     6241 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     6301 ttgggccgcc tccccgcatc gataccgtcg acctcgagac ctagaaaaac atggagcaat
     6361 cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga
     6421 ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc
     6481 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     6541 ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     6601 ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg gatggtgcta
     6661 caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag agaacacccg
     6721 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tattagagtg
     6781 gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc cggactgtac
     6841 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6901 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6961 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7021 cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg
     7081 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt
     7141 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg
     7201 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg
     7261 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc
     7321 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     7381 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     7441 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     7501 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     7561 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     7621 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     7681 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     7741 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     7801 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     7861 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7921 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     7981 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc
     8041 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     8101 cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa ttaatcatcg
     8161 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
     8221 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
     8281 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
     8341 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
     8401 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
     8461 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
     8521 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
     8581 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     8641 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     8701 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8761 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     8821 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     8881 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     8941 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     9001 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     9061 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     9121 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     9181 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     9241 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     9301 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     9361 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     9421 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     9481 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     9541 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     9601 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     9661 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     9721 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     9781 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     9841 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     9901 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     9961 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
    10021 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
    10081 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
    10141 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
    10201 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
    10261 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
    10321 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
    10381 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
    10441 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
    10501 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
    10561 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
    10621 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
    10681 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
    10741 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
    10801 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
    10861 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
    10921 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
    10981 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
    11041 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
    11101 cc
//
