LOCUS       sequence-393510-       11034 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd gen lentiviral plasmid for expressing golgi localized TurboID 
            with EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11034)
  AUTHORS   Yan T, Julio AR, Villanueva M, Jones AE, Ball AB, Boatner LM, Turmon
            AC, Nguyen KB, Yen SL, Desai HS, Divakaruni AS, Backus KM
  TITLE     Proximity-labeling chemoproteomics defines the subcellular 
            cysteinome and inflammation-responsive mitochondrial redoxome.
  JOURNAL   Cell Chem Biol. 2023 Jul 20;30(7):811-827.e7. doi: 
            10.1016/j.chembiol.2023.06.008. Epub 2023 Jul 6.
  PUBMED    37419112
REFERENCE   2  (bases 1 to 11034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2023.06.008"; journalName: "Cell Chem Biol"; 
            date: "2023-07-20- 20"; volume: "30"; issue: "7"; pages: 
            "811-827.e7"
COMMENT     Sequence Label: FUGW-Golgi-TurboID
FEATURES             Location/Qualifiers
     source          1..11034
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        140..519
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        521..719
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     674..694
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             737..917
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    964..1089
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1582..1815
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2000..2044
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2342..2459
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2524..3735
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3572..3591
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3783..3792
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3789..3791,3792..3794,3795..4505)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3789..3791,3792..3794,3795..4505)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4095..4114)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4442..4463
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4515..4541
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4545..4571
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4575..4601
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4608..5564
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     misc_feature    5628..6216
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5681..5701)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6099..6110)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6219..6235)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6220..6236)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6741..6921
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6947..6964)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6953..7177
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7223..7651
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7310..7329)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7520..7541
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7660..7680)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7665..7994
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7845..7980
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7907..7926
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8042..8089
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8108..8482
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8612..8733
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8649..8668)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8703..8722
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8782..8798)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8782..8798)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8795..8817)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8806..8822
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8830..8836,8837..8854,8855..8860))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8875..8896
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9013..9030)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9184..9772)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9264..9283)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9943..10734,10735..10803))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10804..10908)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10983..11002)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
       61 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
      121 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
      181 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
      241 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
      301 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
      361 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
      421 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
      481 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
      541 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
      601 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
      661 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc
      721 gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
      781 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
      841 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
      901 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
      961 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
     1021 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
     1081 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
     1141 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
     1201 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
     1261 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
     1321 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
     1381 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt
     1441 cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt
     1501 agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag
     1561 agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag
     1621 cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat
     1681 agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact
     1741 cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa
     1801 ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt
     1861 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
     1921 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
     1981 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
     2041 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
     2101 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
     2161 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
     2221 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
     2281 agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta ttcatccaca
     2341 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     2401 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
     2461 gggtttatta cagggacagc agagatccag tttggttaat taacccgtgt cggctccaga
     2521 tctggcctcc gcgccgggtt ttggcgcctc ccgcgggcgc ccccctcctc acggcgagcg
     2581 ctgccacgtc agacgaaggg cgcagcgagc gtcctgatcc ttccgcccgg acgctcagga
     2641 cagcggcccg ctgctcataa gactcggcct tagaacccca gtatcagcag aaggacattt
     2701 taggacggga cttgggtgac tctagggcac tggttttctt tccagagagc ggaacaggcg
     2761 aggaaaagta gtcccttctc ggcgattctg cggagggatc tccgtggggc ggtgaacgcc
     2821 gatgattata taaggacgcg ccgggtgtgg cacagctagt tccgtcgcag ccgggatttg
     2881 ggtcgcggtt cttgtttgtg gatcgctgtg atcgtcactt ggtgagtagc gggctgctgg
     2941 gctggccggg gctttcgtgg ccgccgggcc gctcggtggg acggaagcgt gtggagagac
     3001 cgccaagggc tgtagtctgg gtccgcgagc aaggttgccc tgaactgggg gttgggggga
     3061 gcgcagcaaa atggcggctg ttcccgagtc ttgaatggaa gacgcttgtg aggcgggctg
     3121 tgaggtcgtt gaaacaaggt ggggggcatg gtgggcggca agaacccaag gtcttgaggc
     3181 cttcgctaat gcgggaaagc tcttattcgg gtgagatggg ctggggcacc atctggggac
     3241 cctgacgtga agtttgtcac tgactggaga actcggtttg tcgtctgttg cgggggcggc
     3301 agttatggcg gtgccgttgg gcagtgcacc cgtacctttg ggagcgcgcg ccctcgtcgt
     3361 gtcgtgacgt cacccgttct gttggcttat aatgcagggt ggggccacct gccggtaggt
     3421 gtgcggtagg cttttctccg tcgcaggacg cagggttcgg gcctagggta ggctctcctg
     3481 aatcgacagg cgccggacct ctggtgaggg gagggataag tgaggcgtca gtttctttgg
     3541 tcggttttat gtacctatct tcttaagtag ctgaagctcc ggttttgaac tatgcgctcg
     3601 gggttggcga gtgtgttttg tgaagttttt taggcacctt ttgaaatgta atcatttggg
     3661 tcaatatgta attttcagtg ttagactagt aaattgtccg ctaaattctg gccgtttttg
     3721 gcttttttgt tagacgaagc ttgggctgca ggtcgactct agaggatccc cgggtaccgg
     3781 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     3841 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     3901 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     3961 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     4021 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     4081 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     4141 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     4201 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     4261 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     4321 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     4381 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     4441 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     4501 acaagggcag cggctacccg tatgatgttc cggattacgc tggctatccc tacgacgtgc
     4561 ccgactatgc cgggtacccc tatgacgtcc cagactacgc agctagcaaa gacaatactg
     4621 tgcctctgaa gctgatcgct ctcctggcta atggcgagtt ccatagtggc gaacagctgg
     4681 gagaaaccct gggcatgtcc agggccgcta tcaacaagca cattcagact ctgcgcgact
     4741 ggggcgtgga cgtgttcacc gtgcccggaa agggctactc tctgcccgag cctatcccgc
     4801 tgctgaacgc taaacagatt ctgggacagc tggacggcgg gagcgtggca gtcctgcctg
     4861 tggtcgactc caccaatcag tacctgctgg atcgaatcgg cgagctgaag agtggggatg
     4921 cttgcattgc agaatatcag caggcaggga gaggaagcag agggaggaaa tggttctctc
     4981 cttttggagc taacctgtac ctgagtatgt tttggcgcct gaagcgggga ccagcagcaa
     5041 tcggcctggg cccggtcatc ggaattgtca tggcagaagc gctgcgaaag ctgggagcag
     5101 acaaggtgcg agtcaaatgg cccaatgacc tgtatctgca ggatagaaag ctggcaggca
     5161 tcctggtgga gctggccgga ataacaggcg atgctgcaca gatcgtcatt ggcgccggga
     5221 ttaacgtggc tatgaggcgc gtggaggaaa gcgtggtcaa tcagggctgg atcacactgc
     5281 aggaagcagg gattaacctg gacaggaata ctctggccgc tacgctgatc cgagagctgc
     5341 gggcagccct ggaactgttc gagcaggaag gcctggctcc atatctgcca cggtgggaga
     5401 agctggataa cttcatcaat agacccgtga agctgatcat tggggacaaa gagattttcg
     5461 ggattagccg ggggattgat aaacagggag ccctgctgct ggaacaggac ggagttatca
     5521 aaccctggat gggcggagaa atcagtctgc ggtctgccga aaaggagggc ttcctttggc
     5581 ggattttctg ttttcggaag tgagaattcg atatcaagct tatcgataat caacctctgg
     5641 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     5701 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     5761 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     5821 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     5881 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     5941 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     6001 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     6061 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     6121 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     6181 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagacc
     6241 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     6301 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     6361 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     6421 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     6481 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
     6541 tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca
     6601 atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     6661 agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag
     6721 agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     6781 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     6841 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     6901 cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct
     6961 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     7021 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     7081 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     7141 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     7201 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     7261 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     7321 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     7381 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     7441 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     7501 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     7561 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     7621 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     7681 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     7741 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     7801 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     7861 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     7921 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     7981 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     8041 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     8101 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     8161 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     8221 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     8281 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     8341 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     8401 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     8461 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
     8521 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     8581 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     8641 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8701 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     8761 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8821 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8881 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8941 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     9001 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     9061 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     9121 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     9181 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     9241 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     9301 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     9361 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9421 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9481 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9541 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9601 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     9661 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9721 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9781 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9841 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9901 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9961 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    10021 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    10081 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    10141 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    10201 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    10261 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    10321 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    10381 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    10441 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    10501 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    10561 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    10621 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    10681 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10741 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10801 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    10861 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10921 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
    10981 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgt
//
