LOCUS       sequence-393511-       11117 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3rd gen lentiviral plasmid for expressing mitochondria localized 
            TurboID with EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11117)
  AUTHORS   Yan T, Julio AR, Villanueva M, Jones AE, Ball AB, Boatner LM, Turmon
            AC, Nguyen KB, Yen SL, Desai HS, Divakaruni AS, Backus KM
  TITLE     Proximity-labeling chemoproteomics defines the subcellular 
            cysteinome and inflammation-responsive mitochondrial redoxome.
  JOURNAL   Cell Chem Biol. 2023 Jul 20;30(7):811-827.e7. doi: 
            10.1016/j.chembiol.2023.06.008. Epub 2023 Jul 6.
  PUBMED    37419112
REFERENCE   2  (bases 1 to 11117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2023.06.008"; journalName: "Cell Chem Biol"; 
            date: "2023-07-20- 20"; volume: "30"; issue: "7"; pages: 
            "811-827.e7"
COMMENT     Sequence Label: FUGW-Mito-TurboID
FEATURES             Location/Qualifiers
     source          1..11117
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        117..496
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        498..696
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     651..671
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             714..894
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    941..1066
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1559..1792
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1977..2021
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2319..2436
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2501..3712
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3549..3568
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             3908..4864
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             4871..4912
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     regulatory      4922..4931
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4928..4930,4931..4933,4934..5647)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4928..4930,4931..4933,4934..5644)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5234..5253)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5581..5602
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5688..6276
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5741..5761)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6159..6170)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6279..6295)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6280..6296)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6801..6981
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7007..7024)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7013..7237
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7283..7711
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7370..7389)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7580..7601
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7720..7740)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7725..8054
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7905..8040
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7967..7986
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8102..8149
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8168..8542
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8672..8793
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8709..8728)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8763..8782
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8842..8858)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8842..8858)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8855..8877)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8866..8882
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8890..8896,8897..8914,8915..8920))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8935..8956
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9073..9090)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9244..9832)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9324..9343)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10003..10794,10795..10863))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10864..10968)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11043..11062)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
       61 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      121 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      181 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      241 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      301 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      361 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      421 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      481 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      541 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      601 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      661 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
      721 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      781 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      841 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      901 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
      961 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     1021 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     1081 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     1141 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     1201 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     1261 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     1321 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     1381 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     1441 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     1501 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     1561 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     1621 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     1681 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     1741 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     1801 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     1861 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     1921 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     1981 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     2041 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     2101 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     2161 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     2221 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg
     2281 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     2341 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2401 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     2461 gatccagttt ggttaattaa cccgtgtcgg ctccagatct ggcctccgcg ccgggttttg
     2521 gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc
     2581 agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac
     2641 tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct
     2701 agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc
     2761 gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg
     2821 ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat
     2881 cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct ttcgtggccg
     2941 ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc
     3001 cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg gcggctgttc
     3061 ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa acaaggtggg
     3121 gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg ggaaagctct
     3181 tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt ttgtcactga
     3241 ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg ccgttgggca
     3301 gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac ccgttctgtt
     3361 ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt ttctccgtcg
     3421 caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc cggacctctg
     3481 gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta cctatcttct
     3541 taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg tgttttgtga
     3601 agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt ttcagtgtta
     3661 gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag acgaagcttg
     3721 ggctgcaggt cgactctaga ggatccgggc cgccaccatg ctggccaccc gcgtgttcag
     3781 cctggtgggc aagcgcgcca tcagcaccag cgtgtgcgtg cgcgcccacg gatcaggaag
     3841 cggcggccgc ggatcaggaa gcggatcagg aagcggatca ggaagcggat caggaagcgg
     3901 atcaggaaaa gacaatactg tgcctctgaa gctgatcgct ctcctggcta atggcgagtt
     3961 ccatagtggc gaacagctgg gagaaaccct gggcatgtcc agggccgcta tcaacaagca
     4021 cattcagact ctgcgcgact ggggcgtgga cgtgttcacc gtgcccggaa agggctactc
     4081 tctgcccgag cctatcccgc tgctgaacgc taaacagatt ctgggacagc tggacggcgg
     4141 gagcgtggca gtcctgcctg tggtcgactc caccaatcag tacctgctgg atcgaatcgg
     4201 cgagctgaag agtggggatg cttgcattgc agaatatcag caggcaggga gaggaagcag
     4261 agggaggaaa tggttctctc cttttggagc taacctgtac ctgagtatgt tttggcgcct
     4321 gaagcgggga ccagcagcaa tcggcctggg cccggtcatc ggaattgtca tggcagaagc
     4381 gctgcgaaag ctgggagcag acaaggtgcg agtcaaatgg cccaatgacc tgtatctgca
     4441 ggatagaaag ctggcaggca tcctggtgga gctggccgga ataacaggcg atgctgcaca
     4501 gatcgtcatt ggcgccggga ttaacgtggc tatgaggcgc gtggaggaaa gcgtggtcaa
     4561 tcagggctgg atcacactgc aggaagcagg gattaacctg gacaggaata ctctggccgc
     4621 tacgctgatc cgagagctgc gggcagccct ggaactgttc gagcaggaag gcctggctcc
     4681 atatctgcca cggtgggaga agctggataa cttcatcaat agacccgtga agctgatcat
     4741 tggggacaaa gagattttcg ggattagccg ggggattgat aaacagggag ccctgctgct
     4801 ggaacaggac ggagttatca aaccctggat gggcggagaa atcagtctgc ggtctgccga
     4861 aaaggctagc ggcaagccca tccccaaccc cctgctgggc ctggacagca ccggaccggt
     4921 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     4981 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     5041 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     5101 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     5161 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     5221 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     5281 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     5341 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     5401 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     5461 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     5521 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     5581 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     5641 caagtaaagc ggccgcgact ctagaattcg atatcaagct tatcgataat caacctctgg
     5701 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     5761 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     5821 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     5881 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     5941 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     6001 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     6061 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     6121 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     6181 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     6241 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagacc
     6301 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     6361 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     6421 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     6481 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     6541 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
     6601 tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca
     6661 atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     6721 agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag
     6781 agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     6841 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     6901 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     6961 cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct
     7021 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     7081 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     7141 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     7201 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     7261 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     7321 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     7381 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     7441 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     7501 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     7561 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     7621 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     7681 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     7741 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     7801 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     7861 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     7921 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     7981 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     8041 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     8101 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     8161 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     8221 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     8281 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     8341 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     8401 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     8461 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     8521 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
     8581 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     8641 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     8701 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8761 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     8821 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8881 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8941 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     9001 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     9061 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     9121 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     9181 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     9241 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     9301 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     9361 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     9421 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9481 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9541 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9601 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9661 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     9721 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9781 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9841 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9901 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9961 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
    10021 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    10081 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    10141 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    10201 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    10261 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    10321 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    10381 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    10441 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    10501 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    10561 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    10621 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    10681 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    10741 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10801 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10861 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    10921 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10981 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
    11041 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
    11101 gaggtcgctg agtagtg
//
