LOCUS       sequence-393531-        9861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes for CRISPR-Cas12a (EnAsCpf1) with gRNA for targeting the 
            CLYBL safe harbor site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9861)
  AUTHORS   Agarwal D, Dash N, Mazo KW, Chopra M, Avila MP, Patel A, Wong RM, 
            Jia C, Do H, Cheng J, Chiang C, Jurlina SL, Roshan M, Perry MW, Rho 
            JM, Broyer R, Lee CD, Weinreb RN, Gavrilovici C, Oesch NW, Welsbie 
            DS, Wahlin KJ
  TITLE     Human retinal ganglion cell neurons generated by synchronous BMP 
            inhibition and transcription factor mediated reprogramming.
  JOURNAL   NPJ Regen Med. 2023 Sep 29;8(1):55. doi: 10.1038/s41536-023-00327-x.
  PUBMED    37773257
REFERENCE   2  (bases 1 to 9861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "NPJ Regen 
            Med."; date: "2023-09-29"; volume: "8(1):55. doi"; pages: " 
            10.1038/s41536-023-00327-x"
COMMENT     Sequence Label: P789_pY026_EnAsCpf1_CLYBL_T1_catRNA
FEATURES             Location/Qualifiers
     source          1..9861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        170..418
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     170..190
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     341..360
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        632..1011
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1012..1215
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1166..1186
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1260..1279
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1260..1278
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1326..5246
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYRNRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLARGWDVNREKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     regulatory      4966..4975
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5247..5294
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5301..5381
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(5455..5472)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5461..5685
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5731..6159
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5818..5837)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6028..6049
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6168..6188)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6173..6502
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6353..6488
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6415..6434
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6569..7363
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6623..6642)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7233..7252
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7537..7658
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7574..7593)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7628..7647
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7707..7723)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7707..7723)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7720..7742)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7731..7747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7755..7761,7762..7779,7780..7785))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7800..7821
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7938..7955)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8109..8694)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8189..8208)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8865..9656,9657..9725))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9488..9507
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9726..9830)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga cttaattaag agggcctatt
      181 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat
      241 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
      301 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
      361 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg
      421 ccagtggtct agtggtagaa tagtaccctg ccacggtaca gacccgggtt cgattcccgg
      481 ctggaaatta atttctactc ttgtagatac ttccttactg ttaacttcca tataatttct
      541 actcttgtag attttttcaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
      601 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
      661 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
      721 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      781 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      841 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      901 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      961 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     1021 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     1081 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     1141 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     1201 tatataagca gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt
     1261 aatacgactc actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg
     1321 ccaccatgac acagttcgag ggctttacca acctgtatca ggtgagcaag acactgcggt
     1381 ttgagctgat cccacagggc aagaccctga agcacatcca ggagcagggc ttcatcgagg
     1441 aggacaaggc ccgcaatgat cactacaagg agctgaagcc catcatcgat cggatctaca
     1501 agacctatgc cgaccagtgc ctgcagctgg tgcagctgga ttgggagaac ctgagcgccg
     1561 ccatcgactc ctatagaaag gagaaaaccg aggagacaag gaacgccctg atcgaggagc
     1621 aggccacata tcgcaatgcc atccacgact acttcatcgg ccggacagac aacctgaccg
     1681 atgccatcaa taagagacac gccgagatct acaagggcct gttcaaggcc gagctgttta
     1741 atggcaaggt gctgaagcag ctgggcaccg tgaccacaac cgagcacgag aacgccctgc
     1801 tgcggagctt cgacaagttt acaacctact tctccggctt ttataggaac aggaagaacg
     1861 tgttcagcgc cgaggatatc agcacagcca tcccacaccg catcgtgcag gacaacttcc
     1921 ccaagtttaa ggagaattgt cacatcttca cacgcctgat caccgccgtg cccagcctgc
     1981 gggagcactt tgagaacgtg aagaaggcca tcggcatctt cgtgagcacc tccatcgagg
     2041 aggtgttttc cttccctttt tataaccagc tgctgacaca gacccagatc gacctgtata
     2101 accagctgct gggaggaatc tctcgggagg caggcaccga gaagatcaag ggcctgaacg
     2161 aggtgctgaa tctggccatc cagaagaatg atgagacagc ccacatcatc gcctccctgc
     2221 cacacagatt catccccctg tttaagcaga tcctgtccga taggaacacc ctgtctttca
     2281 tcctggagga gtttaagagc gacgaggaag tgatccagtc cttctgcaag tacaagacac
     2341 tgctgagaaa cgagaacgtg ctggagacag ccgaggccct gtttaacgag ctgaacagca
     2401 tcgacctgac acacatcttc atcagccaca agaagctgga gacaatcagc agcgccctgt
     2461 gcgaccactg ggatacactg aggaatgccc tgtatgagcg gagaatctcc gagctgacag
     2521 gcaagatcac caagtctgcc aaggagaagg tgcagcgcag cctgaagcac gaggatatca
     2581 acctgcagga gatcatctct gccgcaggca aggagctgag cgaggccttc aagcagaaaa
     2641 ccagcgagat cctgtcccac gcacacgccg ccctggatca gccactgcct acaaccctga
     2701 agaagcagga ggagaaggag atcctgaagt ctcagctgga cagcctgctg ggcctgtacc
     2761 acctgctgga ctggtttgcc gtggatgagt ccaacgaggt ggaccccgag ttctctgccc
     2821 ggctgaccgg catcaagctg gagatggagc cttctctgag cttctacaac aaggccagaa
     2881 attatgccac caagaagccc tactccgtgg agaagttcaa gctgaacttt cagatgccta
     2941 cactggccag gggctgggac gtgaataggg agaagaacaa tggcgccatc ctgtttgtga
     3001 agaacggcct gtactatctg ggcatcatgc caaagcagaa gggcaggtat aaggccctga
     3061 gcttcgagcc cacagagaaa accagcgagg gctttgataa gatgtactat gactacttcc
     3121 ctgatgccgc caagatgatc ccaaagtgca gcacccagct gaaggccgtg acagcccact
     3181 ttcagaccca cacaaccccc atcctgctgt ccaacaattt catcgagcct ctggagatca
     3241 caaaggagat ctacgacctg aacaatcctg agaaggagcc aaagaagttt cagacagcct
     3301 acgccaagaa aaccggcgac cagaagggct acagagaggc cctgtgcaag tggatcgact
     3361 tcacaaggga ttttctgtcc aagtatacca agacaacctc tatcgatctg tctagcctgc
     3421 ggccatcctc tcagtataag gacctgggcg agtactatgc cgagctgaat cccctgctgt
     3481 accacatcag cttccagaga atcgccgaga aggagatcat ggatgccgtg gagacaggca
     3541 agctgtacct gttccagatc tataacaagg actttgccaa gggccaccac ggcaagccta
     3601 atctgcacac actgtattgg accggcctgt tttctccaga gaacctggcc aagacaagca
     3661 tcaagctgaa tggccaggcc gagctgttct accgccctaa gtccaggatg aagaggatgg
     3721 cacaccggct gggagagaag atgctgaaca agaagctgaa ggatcagaaa accccaatcc
     3781 ccgacaccct gtaccaggag ctgtacgact atgtgaatca cagactgtcc cacgacctgt
     3841 ctgatgaggc cagggccctg ctgcccaacg tgatcaccaa ggaggtgtct cacgagatca
     3901 tcaaggatag gcgctttacc agcgacaagt tctttttcca cgtgcctatc acactgaact
     3961 atcaggccgc caattcccca tctaagttca accagagggt gaatgcctac ctgaaggagc
     4021 accccgagac acctatcatc ggcatcgatc ggggcgagag aaacctgatc tatatcacag
     4081 tgatcgactc caccggcaag atcctggagc agcggagcct gaacaccatc cagcagtttg
     4141 attaccagaa gaagctggac aacagggaga aggagagggt ggcagcaagg caggcctggt
     4201 ctgtggtggg cacaatcaag gatctgaagc agggctatct gagccaggtc atccacgaga
     4261 tcgtggacct gatgatccac taccaggccg tggtggtgct ggagaacctg aatttcggct
     4321 ttaagagcaa gaggaccggc atcgccgaga aggccgtgta ccagcagttc gagaagatgc
     4381 tgatcgataa gctgaattgc ctggtgctga aggactatcc agcagagaaa gtgggaggcg
     4441 tgctgaaccc ataccagctg acagaccagt tcacctcctt tgccaagatg ggcacccagt
     4501 ctggcttcct gttttacgtg cctgccccat atacatctaa gatcgatccc ctgaccggct
     4561 tcgtggaccc cttcgtgtgg aaaaccatca agaatcacga gagccgcaag cacttcctgg
     4621 agggcttcga ctttctgcac tacgacgtga aaaccggcga cttcatcctg cactttaaga
     4681 tgaacagaaa tctgtccttc cagaggggcc tgcccggctt tatgcctgca tgggatatcg
     4741 tgttcgagaa gaacgagaca cagtttgacg ccaagggcac ccctttcatc gccggcaaga
     4801 gaatcgtgcc agtgatcgag aatcacagat tcaccggcag ataccgggac ctgtatcctg
     4861 ccaacgagct gatcgccctg ctggaggaga agggcatcgt gttcagggat ggctccaaca
     4921 tcctgccaaa gctgctggag aatgacgatt ctcacgccat cgacaccatg gtggccctga
     4981 tccgcagcgt gctgcagatg cggaactcca atgccgccac aggcgaggac tatatcaaca
     5041 gccccgtgcg cgatctgaat ggcgtgtgct tcgactcccg gtttcagaac ccagagtggc
     5101 ccatggacgc cgatgccaat ggcgcctacc acatcgccct gaagggccag ctgctgctga
     5161 atcacctgaa ggagagcaag gatctgaagc tgcagaacgg catctccaat caggactggc
     5221 tggcctacat ccaggagctg cgcaacaaaa ggccggcggc cacgaaaaag gccggccagg
     5281 caaaaaagaa aaagggatcc tacccatacg atgttccaga ttacgcttat ccctacgacg
     5341 tgcctgatta tgcataccca tatgatgtcc ccgactatgc ctaagaattc tgcagatatc
     5401 cagcacagtg gcggccgctc gagtctagag ggcccgttta aacccgctga tcagcctcga
     5461 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     5521 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     5581 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     5641 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     5701 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     5761 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     5821 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     5881 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5941 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     6001 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     6061 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     6121 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     6181 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6241 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     6301 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     6361 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     6421 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     6481 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     6541 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     6601 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     6661 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     6721 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     6781 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     6841 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     6901 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     6961 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     7021 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     7081 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     7141 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     7201 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     7261 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     7321 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     7381 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     7441 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     7501 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     7561 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     7621 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     7681 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     7741 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     7801 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7861 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7921 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7981 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     8041 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     8101 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     8161 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8221 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8281 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     8341 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     8401 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8461 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8521 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     8581 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8641 agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     8701 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     8761 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     8821 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     8881 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8941 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     9001 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     9061 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     9121 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     9181 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     9241 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     9301 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     9361 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     9421 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     9481 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     9541 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     9601 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     9661 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     9721 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     9781 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     9841 cgaaaagtgc cacctgacgt c
//
