LOCUS       sequence-393556-        7173 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 554, Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7173)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 7173)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-LaminB1-10
FEATURES             Location/Qualifiers
     source          1..7173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        16..319
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        320..523
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     474..494
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      562..571
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             568..1995
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     polyA_signal    3916..4037
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3953..3972)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4007..4026
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4044..4499)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4181..4202)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4393..4412
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4526..4630
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4632..4989
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4632..4823
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4655..4675)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4840..4975
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5024..5818
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5024..5818
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5078..5097)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5688..5707
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6006..6025)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6050..6097
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6426..7014
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6915..6934
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
       61 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      121 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      181 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      241 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      301 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      361 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      421 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      481 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca
      541 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggaggt catcaaagag
      601 ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt cgagatcgag
      661 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
      721 ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta cggctccaag
      781 gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt ccccgagggc
      841 ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt gacccaggac
      901 tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac caacttcccc
      961 cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac cgagcgcctg
     1021 tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct gaaggacggc
     1081 ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt gcaactgccc
     1141 ggctactact acgtggacac caagctggac atcacctccc acaacgagga ctacaccatc
     1201 gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctggggca tggcaccggc
     1261 agcaccggca gcggcagctc cggcaccgcc tcctccgagg acaacaacat ggccgtcatc
     1321 aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca cgagttcgag
     1381 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     1441 accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt catgtacggc
     1501 tccaaggcgt acgtgaagca ccccgccgac atccccgatt acaagaagct gtccttcccc
     1561 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc
     1621 caggactcct ccctgcagga cggcacgctg atctacaagg tgaagatgcg cggcaccaac
     1681 ttcccccccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag
     1741 cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc accaggccct gaagctgaag
     1801 gacggcggcc actacctggt ggagttcaag accatctaca tggccaagaa gcccgtgcaa
     1861 ctgcccggct actactacgt ggacaccaag ctggacatca cctcccacaa cgaggactac
     1921 accatcgtgg aacagtacga gcgctccgag ggccgccacc acctgttcct gtacggcatg
     1981 gacgagctgt acaagtccgg actcagatct cgagctcaag cttcgatggc gactgcgacc
     2041 cccgtgccgc cgcggatggg cagccgcgct ggcggcccca ccacgccgct gagccccacg
     2101 cgcctgtcgc ggctccagga gaaggaggag ctgcgcgagc tcaatgaccg gctggcggtg
     2161 tacatcgaca aggtgcgcag cctggagacg gagaacagcg cgctgcagct gcaggtgacg
     2221 gagcgcgagg aggtgcgcgg ccgtgagctc accggcctca aggcgctcta cgagaccgag
     2281 ctggccgacg cgcgacgcgc gctcgacgac acggcccgcg agcgcgccaa gctgcagatc
     2341 gagctgggca agtgcaaggc ggaacacgac cagctgctcc tcaactatgc taagaaggaa
     2401 tctgatctta atggcgccca gatcaagctt cgagaatatg aagcagcact caattcgaaa
     2461 gatgcagctc ttgctactgc acttggtgac aaaaaaagtt tagagggaga tttggaggat
     2521 ctgaaggatc agattgccca gttggaagcc tccttagctg cagccaaaaa acagttagca
     2581 gatgaaactt tacttaaagt agatttggag aatcgttgtc agagccttac tgaggacttg
     2641 gagtttcgca aaagcatgta tgaagaggag attaacgaga ccagaaggaa gcatgaaacg
     2701 cgcttggtag aggtggattc tgggcgtcaa attgagtatg agtacaagct ggcgcaagcc
     2761 cttcatgaga tgagagagca acatgatgcc caagtgaggc tgtataagga ggagctggag
     2821 cagacttacc atgccaaact tgagaatgcc agactgtcat cagagatgaa tacttctact
     2881 gtcaacagtg ccagggaaga actgatggaa agccgcatga gaattgagag cctttcatcc
     2941 cagctttcta atctacagaa agagtctaga gcatgtttgg aaaggattca agaattagag
     3001 gacttgcttg ctaaagaaaa agacaactct cgtcgcatgc tgacagacaa agagagagag
     3061 atggcggaaa taagggatca aatgcagcaa cagctgaatg actatgaaca gcttcttgat
     3121 gtaaagttag ccctggacat ggaaatcagt gcttacagga aactcttaga aggcgaagaa
     3181 gagaggttga agctgtctcc aagcccttct tcccgtgtga cagtatcccg agcatcctca
     3241 agtcgtagtg tacgtacaac tagaggaaag cggaagaggg ttgatgtgga agaatcagag
     3301 gcgagtagta gtgttagcat ctctcattcc gcctcagcca ctggaaatgt ttgcatcgaa
     3361 gaaattgatg ttgatgggaa atttatccgc ttgaagaaca cttctgaaca ggatcaacca
     3421 atgggaggct gggagatgat cagaaaaatt ggagacacat cagtcagtta taaatatacc
     3481 tcaagatatg tgctgaaggc aggccagact gttacaattt gggctgcaaa cgctggtgtc
     3541 acagccagcc ccccaactga cctcatctgg aagaaccaga actcgtgggg cactggcgaa
     3601 gatgtgaagg ttatattgaa aaattctcag ggagaggagg ttgctcaaag aagtacagtc
     3661 tttaaaacaa ccatacctga agaagaggag gaggaggaag aagcagctgg agtggttgtt
     3721 gaggaagaac ttttccacca gcagggaacc ccaagagcat ccaatagaag ctgtgcaatt
     3781 atgtagggat ccaccggatc tagataactg atcataatca gccataccac atttgtagag
     3841 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     3901 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     3961 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     4021 ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     4081 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     4141 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     4201 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     4261 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     4321 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     4381 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     4441 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     4501 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4561 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     4621 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     4681 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     4741 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     4801 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     4861 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     4921 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     4981 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     5041 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     5101 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     5161 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     5221 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     5281 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     5341 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     5401 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     5461 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     5521 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     5581 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     5641 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     5701 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     5761 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     5821 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     5881 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     5941 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     6001 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     6061 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     6121 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     6181 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     6241 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     6301 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6361 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6421 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6481 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6541 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     6601 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6661 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6721 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6781 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6841 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6901 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6961 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     7021 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     7081 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta gttattaata
     7141 gtaatcaatt acggggtcat tagttcatag ccc
//
