LOCUS       sequence-393564-       10414 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human ALK receptor tyrosine kinase in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10414)
  AUTHORS   Gudernova I, Foldynova-Trantirkova S, Ghannamova BE, Fafilek B, 
            Varecha M, Balek L, Hruba E, Jonatova L, Jelinkova I, Kunova 
            Bosakova M, Trantirek L, Mayer J, Krejci P
  TITLE     One reporter for in-cell activity profiling of majority of protein 
            kinase oncogenes.
  JOURNAL   Elife. 2017 Feb 15;6. pii: e21536. doi: 10.7554/eLife.21536.
  PUBMED    28199182
REFERENCE   2  (bases 1 to 10414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-02-15"; volume: "6. pii: e21536. doi"; pages: " 
            10.7554/eLife.21536"
COMMENT     Sequence Label: pcDNA3.1-ALK-V5/HIS
FEATURES             Location/Qualifiers
     source          1..10414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5912..5953
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5963..5980
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6003..6020)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6009..6233
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6279..6707
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6366..6385)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6576..6597
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6716..6736)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6721..7050
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6901..7036
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6963..6982
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7117..7911
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7171..7190)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7781..7800
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8087..8208
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8124..8143)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8178..8197
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8257..8273)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8257..8273)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8270..8292)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8281..8297
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8305..8311,8312..8329,8330..8335))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8350..8371
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8488..8505)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8659..9247)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8739..8758)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9418..10209,10210..10278))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10041..10060
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10279..10383)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattgcc cttcaccagc
      961 agaagggccg tcaaggccca ccatgggagc catcgggctc ctgtggctcc tgccgctgct
     1021 gctttccacg gcagctgtgg gctccgggat ggggaccggc cagcgcgcgg gctccccagc
     1081 tgcggggccg ccgctgcagc cccgggagcc actcagctac tcgcgcctgc agaggaagag
     1141 tctggcagtt gacttcgtgg tgccctcgct cttccgtgtc tacgcccggg acctactgct
     1201 gccaccatcc tcctcggagc tgaaggctgg caggcccgag gcccgcggct cgctagctct
     1261 ggactgcgcc ccgctgctca ggttgctggg gccggcgccg ggggtctcct ggaccgccgg
     1321 ttcaccagcc ccggcagagg cccggacgct gtccagggtg ctgaagggcg gctccgtgcg
     1381 caagctccgg cgtgccaagc agttggtgct ggagctgggc gaggaggcga tcttggaggg
     1441 ttgcgtcggg ccccccgggg aggcggctgt ggggctgctc cagttcaatc tcagcgagct
     1501 gttcagttgg tggattcgcc aaggcgaagg gcgactgagg atccgcctga tgcccgagaa
     1561 gaaggcgtcg gaagtgggca gagagggaag gctgtccgcg gcaattcgcg cctcccagcc
     1621 ccgccttctc ttccagatct tcgggactgg tcatagctcc ttggaatcac caacaaacat
     1681 gccttctcct tctcctgatt attttacatg gaatctcacc tggataatga aagactcctt
     1741 ccctttcctg tctcatcgca gccgatatgg tctggagtgc agctttgact tcccctgtga
     1801 gctggagtat tcccctccac tgcatgacct caggaaccag agctggtcct ggcgccgcat
     1861 cccctccgag gaggcctccc agatggactt gctggatggg cctggggcag agcgttctaa
     1921 ggagatgccc agaggctcct ttctccttct caacacctca gctgactcca agcacaccat
     1981 cctgagtccg tggatgagga gcagcagtga gcactgcaca ctggccgtct cggtgcacag
     2041 gcacctgcag ccctctggaa ggtacattgc ccagctgctg ccccacaacg aggctgcaag
     2101 agagatcctc ctgatgccca ctccagggaa gcatggttgg acagtgctcc agggaagaat
     2161 cgggcgtcca gacaacccat ttcgagtggc cctggaatac atctccagtg gaaaccgcag
     2221 cttgtctgca gtggacttct ttgccctgaa gaactgcagt gaaggaacat ccccaggctc
     2281 caagatggcc ctgcagagct ccttcacttg ttggaatggg acagtcctcc agcttgggca
     2341 ggcctgtgac ttccaccagg actgtgccca gggagaagat gagagccaga tgtgccggaa
     2401 actgcctgtg ggtttttact gcaactttga agatggcttc tgtggctgga cccaaggcac
     2461 actgtcaccc cacactcctc aatggcaggt caggacccta aaggatgccc ggttccagga
     2521 ccaccaagac catgctctat tgctcagtac cactgatgtc cccgcttctg aaagtgctac
     2581 agtgaccagt gctacgtttc ctgcaccgat caagagctct ccatgtgagc tccgaatgtc
     2641 ctggctcatt cgtggagtct tgaggggaaa cgtgtccttg gtgctagtgg agaacaaaac
     2701 cgggaaggag caaggcagga tggtctggca tgtcgccgcc tatgaaggct tgagcctgtg
     2761 gcagtggatg gtgttgcctc tcctcgatgt gtctgacagg ttctggctgc agatggtcgc
     2821 atggtgggga caaggatcca gagccatcgt ggcttttgac aatatctcca tcagcctgga
     2881 ctgctacctc accattagcg gagaggacaa gatcctgcag aatacagcac ccaaatcaag
     2941 aaacctgttt gagagaaacc caaacaagga gctgaaaccc ggggaaaatt caccaagaca
     3001 gacccccatc tttgacccta cagttcattg gctgttcacc acatgtgggg ccagcgggcc
     3061 ccatggcccc acccaggcac agtgcaacaa cgcctaccag aactccaacc tgagcgtgga
     3121 ggtggggagc gagggccccc tgaaaggcat ccagatctgg aaggtgccag ccaccgacac
     3181 ctacagcatc tcgggctacg gagctgctgg cgggaaaggc gggaagaaca ccatgatgcg
     3241 gtcccacggc gtgtctgtgc tgggcatctt caacctggag aaggatgaca tgctgtacat
     3301 cctggttggg cagcagggag aggacgcctg ccccagtaca aaccagttaa tccagaaagt
     3361 ctgcattgga gagaacaatg tgatagaaga agaaatccgt gtgaacagaa gcgtgcatga
     3421 gtgggcagga ggcggaggag gagggggtgg agccacctac gtatttaaga tgaaggatgg
     3481 agtgccggtg cccctgatca ttgcagccgg aggtggtggc agggcctacg gggccaagac
     3541 agacacgttc cacccagaga gactggagaa taactcctcg gttctagggc taaacggcaa
     3601 ttccggagcc gcaggtggtg gaggtggctg gaatgataac acttccttgc tctgggccgg
     3661 aaaatctttg caggagggtg ccaccggagg acattcctgc ccccaggcca tgaagaagtg
     3721 ggggtgggag acaagagggg gtttcggagg gggtggaggg gggtgctcct caggtggagg
     3781 aggcggagga tatataggcg gcaatgcagc ctcaaacaat gaccccgaaa tggatgggga
     3841 agatggggtt tccttcatca gtccactggg catcctgtac accccagctt taaaagtgat
     3901 ggaaggccac ggggaagtga atattaagca ttatctaaac tgcagtcact gtgaggtaga
     3961 cgaatgtcac atggaccctg aaagccacaa ggtcatctgc ttctgtgacc acgggacggt
     4021 gctggctgag gatggcgtct cctgcattgt gtcacccacc ccggagccac acctgccact
     4081 ctcgctgatc ctctctgtgg tgacctctgc cctcgtggcc gccctggtcc tggctttctc
     4141 cggcatcatg attgtgtacc gccggaagca ccaggagctg caagccatgc agatggagct
     4201 gcagagccct gagtacaagc tgagcaagct ccgcacctcg accatcatga ccgactacaa
     4261 ccccaactac tgctttgctg gcaagacctc ctccatcagt gacctgaagg aggtgccgcg
     4321 gaaaaacatc accctcattc ggggtctggg ccatggcgcc tttggggagg tgtatgaagg
     4381 ccaggtgtcc ggaatgccca acgacccaag ccccctgcaa gtggctgtga agacgctgcc
     4441 tgaagtgtgc tctgaacagg acgaactgga tttcctcatg gaagccctga tcatcagcaa
     4501 attcaaccac cagaacattg ttcgctgcat tggggtgagc ctgcaatccc tgccccggtt
     4561 catcctgctg gagctcatgg cggggggaga cctcaagtcc ttcctccgag agacccgccc
     4621 tcgcccgagc cagccctcct ccctggccat gctggacctt ctgcacgtgg ctcgggacat
     4681 tgcctgtggc tgtcagtatt tggaggaaaa ccacttcatc caccgagaca ttgctgccag
     4741 aaactgcctc ttgacctgtc caggccctgg aagagtggcc aagattggag acttcgggat
     4801 ggcccgagac atctacaggg cgagctacta tagaaaggga ggctgtgcca tgctgccagt
     4861 taagtggatg cccccagagg ccttcatgga aggaatattc acttctaaaa cagacacatg
     4921 gtcctttgga gtgctgctat gggaaatctt ttctcttgga tatatgccat accccagcaa
     4981 aagcaaccag gaagttctgg agtttgtcac cagtggaggc cggatggacc cacccaagaa
     5041 ctgccctggg cctgtatacc ggataatgac tcagtgctgg caacatcagc ctgaagacag
     5101 gcccaacttt gccatcattt tggagaggat tgaatactgc acccaggacc cggatgtaat
     5161 caacaccgct ttgccgatag aatatggtcc acttgtggaa gaggaagaga aagtgcctgt
     5221 gaggcccaag gaccctgagg gggttcctcc tctcctggtc tctcaacagg caaaacggga
     5281 ggaggagcgc agcccagctg ccccaccacc tctgcctacc acctcctctg gcaaggctgc
     5341 aaagaaaccc acagctgcag agatctctgt tcgagtccct agagggccgg ccgtggaagg
     5401 gggacacgtg aatatggcat tctctcagtc caaccctcct tcggagttgc acaaggtcca
     5461 cggatccaga aacaagccca ccagcttgtg gaacccaacg tacggctcct ggtttacaga
     5521 gaaacccacc aaaaagaata atcctatagc aaagaaggag ccacacgaca ggggtaacct
     5581 ggggctggag ggaagctgta ctgtcccacc taacgttgca actgggagac ttccgggggc
     5641 ctcactgctc ctagagccct cttcgctgac tgccaatatg aaggaggtac ctctgttcag
     5701 gctacgtcac ttcccttgtg ggaatgtcaa ttacggctac cagcaacagg gcttgccctt
     5761 agaagccgct actgcccctg gagctggtca ttacgaggat accattctga aaagcaagaa
     5821 tagcatgaac cagcctgggc ccgcaaaggg caattctgca gatatccagc acagtggcgg
     5881 ccgctcgagt ctagagggcc cgcggttcga aggtaagcct atccctaacc ctctcctcgg
     5941 tctcgattct acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc
     6001 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     6061 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     6121 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     6181 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     6241 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     6301 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     6361 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     6421 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     6481 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     6541 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     6601 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     6661 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     6721 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     6781 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     6841 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     6901 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     6961 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     7021 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     7081 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     7141 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     7201 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     7261 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     7321 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     7381 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     7441 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     7501 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     7561 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     7621 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     7681 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     7741 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     7801 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     7861 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     7921 tggggttcgc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     7981 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     8041 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     8101 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     8161 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     8221 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     8281 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     8341 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8401 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8461 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8521 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8581 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8641 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8701 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8761 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8821 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8881 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8941 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9001 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9061 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     9121 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9181 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9241 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9301 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9361 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9421 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     9481 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     9541 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     9601 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     9661 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     9721 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     9781 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     9841 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9901 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9961 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10021 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10081 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10141 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10201 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10261 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10321 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10381 gcgcacattt ccccgaaaag tgccacctga cgtc
//
