LOCUS       sequence-393572-       17468 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pTRANS T-DNA vector with nptII selection and 35S:Cas9 with 6x 
            tRNA-spliced gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd 
            and MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17468)
  AUTHORS   Miller SS, Dornbusch MR, Farmer AD, Huertas R, Gutierrez-Gonzalez 
            JJ, Young ND, Samac DA, Curtin SJ
  TITLE     Alfalfa (Medicago sativa L.) pho2 mutant plants hyperaccumulate 
            phosphate.
  JOURNAL   G3 (Bethesda). 2022 May 30;12(6). pii: 6574359. doi: 
            10.1093/g3journal/jkac096.
  PUBMED    35471600
REFERENCE   2  (bases 1 to 17468)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2022-05-30"; volume: "12(6). pii: 6574359. 
            doi"; pages: " 10.1093/g3journal/jkac096"
COMMENT     Sequence Label: pTRANS-Pho2-1/2-2-tRNA
FEATURES             Location/Qualifiers
     source          1..17468
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1843..2640)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1954..1973)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2564..2583
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2703..3379)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    3570..3591
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3606..3611,3612..3629,3630..3636)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3644..3660
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3649..3671
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3668..3684
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3668..3684
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        4214..4559
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             4593..8696
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4593..8696
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             8709..8729
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      8742..8990
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     misc_RNA        9588..9663
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        9761..9836
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        9934..10009
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        10107..10182
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        10280..10355
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        10453..10528
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     polyA_signal    10625..10799
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     10846..10862
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12479..12496)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12479..12495)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12488..12510)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    12698..12722
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             14023..14652
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             15081..16154
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      16220..16414
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(16650..16672)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    16758..16898
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     16813..16832
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(16913..16930)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(17084..17468,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(17164..17183)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
     1801 tcgactctag ctagaggatc gatccgaacc ccagagtccc gctcagaaga actcgtcaag
     1861 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
     1921 gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc
     1981 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
     2041 ttccaccatg atattcggca agcaggcatc gccatgtgtc acgacgagat cctcgccgtc
     2101 gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc
     2161 gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg
     2221 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
     2281 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg
     2341 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
     2401 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctggag
     2461 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
     2521 cagccgaaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa
     2581 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatccccat
     2641 ggtcgatcga cagatctgcg aaagctcgag agagatagat ttgtagagag agactggtga
     2701 tttcagcgtg tcctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa
     2761 ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc
     2821 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
     2881 tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga
     2941 tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct
     3001 gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc
     3061 ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc
     3121 accatgttca catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac
     3181 gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac
     3241 gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt
     3301 ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac
     3361 cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt
     3421 ggagtagacg agagtgtcgt gctccaccat gttggcaagc tgctctagcc aatacgcaaa
     3481 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3541 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     3601 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     3661 tttcacacag gaaacagcta tgacatgatt acgaattcga gctcggtacc cggggatcgg
     3721 cgcgccagat ttgccttttc aatttcagaa agaatgctaa cccacagatg gttagagagg
     3781 cttacgcagc aggtatcatc aagacgatct acccgagcaa taatctccag gaaatcaaat
     3841 accttcccaa gaaggttaaa gatgcagtca aaagattcag gactaactgc atcaagaaca
     3901 cagagaaaga tatatttctc aagatcagaa gtactattcc agtatggacg attcaaggct
     3961 tgcttcacaa accaaggcaa gtaatagaga ttggagtctc taaaaaggta gttcccactg
     4021 aatcaaaggc catggagtca aagattcaaa tagaggacct aacagaactc gccgtaaaga
     4081 ctggcgaaca gttcatacag agtctcttac gactcaatga caagaagaaa atcttcgtca
     4141 acatggtgga gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag
     4201 accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc
     4261 attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca
     4321 aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc
     4381 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt
     4441 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
     4501 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagagaacac
     4561 gggggactcc tgcaggtaga tcgctcgtcg acatggataa gaagtactct atcggactcg
     4621 atatcggaac taactctgtg ggatgggctg tgatcaccga tgagtacaag gtgccatcta
     4681 agaagttcaa ggttctcgga aacaccgata ggcactctat caagaaaaac cttatcggtg
     4741 ctctcctctt cgattctggt gaaactgctg aggctaccag actcaagaga accgctagaa
     4801 gaaggtacac cagaagaaag aacaggatct gctacctcca agagatcttc tctaacgaga
     4861 tggctaaagt ggatgattca ttcttccaca ggctcgaaga gtcattcctc gtggaagaag
     4921 ataagaagca cgagaggcac cctatcttcg gaaacatcgt tgatgaggtg gcataccacg
     4981 agaagtaccc tactatctac cacctcagaa agaagctcgt tgattctact gataaggctg
     5041 atctcaggct catctacctc gctctcgctc acatgatcaa gttcagagga cacttcctca
     5101 tcgagggtga tctcaaccct gataactctg atgtggataa gttgttcatc cagctcgtgc
     5161 agacctacaa ccagcttttc gaagagaacc ctatcaacgc ttcaggtgtg gatgctaagg
     5221 ctatcctctc tgctaggctc tctaagtcaa gaaggcttga gaacctcatt gctcagctcc
     5281 ctggtgagaa gaagaacgga cttttcggaa acttgatcgc tctctctctc ggactcaccc
     5341 ctaacttcaa gtctaacttc gatctcgctg aggatgcaaa gctccagctc tcaaaggata
     5401 cctacgatga tgatctcgat aacctcctcg ctcagatcgg agatcagtac gctgatttgt
     5461 tcctcgctgc taagaacctc tctgatgcta tcctcctcag tgatatcctc agagtgaaca
     5521 ccgagatcac caaggctcca ctctcagctt ctatgatcaa gagatacgat gagcaccacc
     5581 aggatctcac acttctcaag gctcttgtta gacagcagct cccagagaag tacaaagaga
     5641 ttttcttcga tcagtctaag aacggatacg ctggttacat cgatggtggt gcatctcaag
     5701 aagagttcta caagttcatc aagcctatcc tcgagaagat ggatggaacc gaggaactcc
     5761 tcgtgaagct caatagagag gatcttctca gaaagcagag gaccttcgat aacggatcta
     5821 tccctcatca gatccacctc ggagagttgc acgctatcct tagaaggcaa gaggatttct
     5881 acccattcct caaggataac agggaaaaga ttgagaagat tctcaccttc agaatccctt
     5941 actacgtggg acctctcgct agaggaaact caagattcgc ttggatgacc agaaagtctg
     6001 aggaaaccat caccccttgg aacttcgaag aggtggtgga taagggtgct agtgctcagt
     6061 ctttcatcga gaggatgacc aacttcgata agaaccttcc aaacgagaag gtgctcccta
     6121 agcactcttt gctctacgag tacttcaccg tgtacaacga gttgaccaag gttaagtacg
     6181 tgaccgaggg aatgaggaag cctgcttttt tgtcaggtga gcaaaagaag gctatcgttg
     6241 atctcttgtt caagaccaac agaaaggtga ccgtgaagca gctcaaagag gattacttca
     6301 agaaaatcga gtgcttcgat tcagttgaga tttctggtgt tgaggatagg ttcaacgcat
     6361 ctctcggaac ctaccacgat ctcctcaaga tcattaagga taaggatttc ttggataacg
     6421 aggaaaacga ggatatcttg gaggatatcg ttcttaccct caccctcttt gaagatagag
     6481 agatgattga agaaaggctc aagacctacg ctcatctctt cgatgataag gtgatgaagc
     6541 agttgaagag aagaagatac actggttggg gaaggctctc aagaaagctc attaacggaa
     6601 tcagggataa gcagtctgga aagacaatcc ttgatttcct caagtctgat ggattcgcta
     6661 acagaaactt catgcagctc atccacgatg attctctcac ctttaaagag gatatccaga
     6721 aggctcaggt ttcaggacag ggtgatagtc tccatgagca tatcgctaac ctcgctggat
     6781 ctcctgcaat caagaaggga atcctccaga ctgtgaaggt tgtggatgag ttggtgaagg
     6841 tgatgggaag gcataagcct gagaacatcg tgatcgaaat ggctagagag aaccagacca
     6901 ctcagaaggg acagaagaac tctagggaaa ggatgaagag gatcgaggaa ggtatcaaag
     6961 agcttggatc tcagatcctc aaagagcacc ctgttgagaa cactcagctc cagaatgaga
     7021 agctctacct ctactacctc cagaacggaa gggatatgta tgtggatcaa gagttggata
     7081 tcaacaggct ctctgattac gatgttgatc atatcgtgcc acagtcattc ttgaaggatg
     7141 attctatcga taacaaggtg ctcaccaggt ctgataagaa caggggtaag agtgataacg
     7201 tgccaagtga agaggttgtg aagaaaatga agaactattg gaggcagctc ctcaacgcta
     7261 agctcatcac tcagagaaag ttcgataact tgactaaggc tgagagggga ggactctctg
     7321 aattggataa ggcaggattc atcaagaggc agcttgtgga aaccaggcag atcactaagc
     7381 acgttgcaca gatcctcgat tctaggatga acaccaagta cgatgagaac gataagttga
     7441 tcagggaagt gaaggttatc accctcaagt caaagctcgt gtctgatttc agaaaggatt
     7501 tccaattcta caaggtgagg gaaatcaaca actaccacca cgctcacgat gcttacctta
     7561 acgctgttgt tggaaccgct ctcatcaaga agtatcctaa gctcgagtca gagttcgtgt
     7621 acggtgatta caaggtgtac gatgtgagga agatgatcgc taagtctgag caagagatcg
     7681 gaaaggctac cgctaagtat ttcttctact ctaacatcat gaatttcttc aagaccgaga
     7741 ttaccctcgc taacggtgag atcagaaaga ggccactcat cgagacaaac ggtgaaacag
     7801 gtgagatcgt gtgggataag ggaagggatt tcgctaccgt tagaaaggtg ctctctatgc
     7861 cacaggtgaa catcgttaag aaaaccgagg tgcagaccgg tggattctct aaagagtcta
     7921 tcctccctaa gaggaactct gataagctca ttgctaggaa gaaggattgg gaccctaaga
     7981 aatacggtgg tttcgattct cctaccgtgg cttactctgt tctcgttgtg gctaaggttg
     8041 agaagggaaa gagtaagaag ctcaagtctg ttaaggaact tctcggaatc actatcatgg
     8101 aaaggtcatc tttcgagaag aacccaatcg atttcctcga ggctaaggga tacaaagagg
     8161 ttaagaagga tctcatcatc aagctcccaa agtactcact cttcgaactc gagaacggta
     8221 gaaagaggat gctcgcttct gctggtgagc ttcaaaaggg aaacgagctt gctctcccat
     8281 ctaagtacgt taactttctt tacctcgctt ctcactacga gaagttgaag ggatctccag
     8341 aagataacga gcagaagcaa cttttcgttg agcagcacaa gcactacttg gatgagatca
     8401 tcgagcagat ctctgagttc tctaaaaggg tgatcctcgc tgatgcaaac ctcgataagg
     8461 tgttgtctgc ttacaacaag cacagagata agcctatcag ggaacaggca gagaacatca
     8521 tccatctctt cacccttacc aacctcggtg ctcctgctgc tttcaagtac ttcgatacaa
     8581 ccatcgatag gaagagatac acctctacca aagaagtgct cgatgctacc ctcatccatc
     8641 agtctatcac tggactctac gagactagga tcgatctctc acagctcggt ggtgattcaa
     8701 gggctgatcc taagaagaag aggaaggttt gacgtcgacg atatgaagat gaagatgaaa
     8761 tatttggtgt gtcaaataaa aagcttgtgt gcttaagttt gtgttttttt cttggcttgt
     8821 tgtgttatga atttgtggct ttttctaata ttaaatgaat gtaagatcac attataatga
     8881 ataaacaaat gtttctataa tccattgtga atgttttgtt ggatctcttc tgcagcatat
     8941 aactactgta tgtgctatgg tatggactat ggaatatgat taaagataag ccagagctct
     9001 ggtgacggac ggcgcgctgg cagacatact gtcccacaaa tgaagatgga atctgtaaaa
     9061 gaaaacgcgt gaaataatgc gtctgacaaa ggttaggtcg gctgccttta atcaatacca
     9121 aagtggtccc taccacgatg gaaaaactgt gcagtcggtt tggctttttc tgacgaacaa
     9181 ataagattcg tggccgacag gtgggggtcc accatgtgaa ggcatcttca gactccaata
     9241 atggagcaat gacgtaaggg cttacgaaat aagtaagggt agtttgggaa atgtccactc
     9301 acccgtcagt ctataaatac ttagcccctc cctcattgtt aagggagcaa aatctcagag
     9361 agatagtcct agagagagaa agagagcaag tagcctagaa gtagtcaagg cggcgaagta
     9421 ttcaggcacg tggccaggaa gaagaaaagc caagacgacg aaaacaggta agagctaagc
     9481 ttcctgcagg aacaaagcac cagtggtcta gtggtagaat agtaccctgc cacggtacag
     9541 acccgggttc gattcccggc tggtgcaatt agaacctaaa ggaaccggtt ttagagctag
     9601 aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
     9661 tgcaacaaag caccagtggt ctagtggtag aatagtaccc tgccacggta cagacccggg
     9721 ttcgattccc ggctggtgca aattgggaag ataaagacca gttttagagc tagaaatagc
     9781 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgcaaca
     9841 aagcaccagt ggtctagtgg tagaatagta ccctgccacg gtacagaccc gggttcgatt
     9901 cccggctggt gcagtttgag tctcctaaat acggttttag agctagaaat agcaagttaa
     9961 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgca acaaagcacc
    10021 agtggtctag tggtagaata gtaccctgcc acggtacaga cccgggttcg attcccggct
    10081 ggtgcaatta tgtaactaag gggccagttt tagagctaga aatagcaagt taaaataagg
    10141 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcaacaaagc accagtggtc
    10201 tagtggtaga atagtaccct gccacggtac agacccgggt tcgattcccg gctggtgcat
    10261 cacacgcaaa ctggcagctg ttttagagct agaaatagca agttaaaata aggctagtcc
    10321 gttatcaact tgaaaaagtg gcaccgagtc ggtgcaacaa agcaccagtg gtctagtggt
    10381 agaatagtac cctgccacgg tacagacccg ggttcgattc ccggctggtg cagttcaaag
    10441 gccgctcgag gagttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
    10501 acttgaaaaa gtggcaccga gtcggtgcaa caaagcacca gtggtctagt ggtagaatag
    10561 taccctgcca cggtacagac ccgggttcga ttcccggctg gtgcagtcga tcgacaagct
    10621 cgagtttctc cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag
    10681 ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat
    10741 acttctatca ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc
    10801 cccgaattag agctctaccg gcgtaatatg gcgcgccgaa gagcgcgagg tcgacggtat
    10861 cgataagctt gatatcgaat tcctgcagcc cgggggatcc gcccgctcta gactgccttg
    10921 taggtgcgaa ttttctctgc tcaaattgtt gaggttagcg gatttgtaaa cgcgtttata
    10981 tgggctgctt ggagggtact tttggattaa tttttttctg ccagcgcatt ctgacgcggc
    11041 accgctttgg aaagtgcgct gtgggtccgc gttttctaca ataatgtgcc gatccggtca
    11101 gaaagtatat ggatgagttg tgccagcctc accaacgtgc tgcaggccca tcatgactac
    11161 ttcaatgtta atgggggtaa tgaataaata ggcgaaattg ggttcacggt gggcccaggg
    11221 aatataatat tgccgcagag gtagtcggat gccaaggccc gcaactaata gttcacgaac
    11281 aaattcattg tagtgggcgg ccaactccaa aaccaattgc cagttattgt attgcaatac
    11341 atatatggct tcctcccttg cctgcaggat gtctgagcca cctcgggctg agacctttgt
    11401 attcctggac ctagaagcca ctgggctccc aaacatggac cctgagattg cagagatatc
    11461 cctttttgct gttcaccgct cttccctgga gaacccagaa cgggatgatt ctggttcctt
    11521 ggtgctgccc cgtgttctgg acaagctcac actgtgcatg tgcccggagc gcccctttac
    11581 tgccaaggcc agtgagatta ctggtttgag cagcgaaagc ctgatgcact gcgggaaggc
    11641 tggtttcaat ggcgctgtgg taaggacact gcagggcttc ctaagccgcc aggagggccc
    11701 catctgcctt gtggcccaca atggcttcga ttatgacttc ccactgctgt gcacggagct
    11761 acaacgtctg ggtgcccatc tgccccaaga cactgtctgc ctggacacac tgcctgcatt
    11821 gcggggcctg gaccgtgctc acagccacgg caccagggct caaggccgca aaagctacag
    11881 cctggccagt ctcttccacc gctacttcca ggctgaaccc agtgctgccc attcagcaga
    11941 aggtgatgtg cacaccctgc ttctgatctt cctgcatcgt gctcctgagc tgctcgcctg
    12001 ggcagatgag caggcccgca gctgggctca tattgagccc atgtacgtgc cacctgatgg
    12061 tccaagcctc gaagcctgat aactcgaggt tcgagtatta tggcattggg aaaactgttt
    12121 ttcttgtacc atttgttgtg cttgtaattt actgtgtttt ttattcggtt ttcgctatcg
    12181 aactgtgaaa tggaaatgga tggagaagag ttaatgaatg atatggtcct tttgttcatt
    12241 ctcaaattaa tattatttgt tttttctctt atttgttgtg tgttgaattt gaaattataa
    12301 gagatatgca aacattttgt tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg
    12361 aagttaatat gaggagtaaa acacttgtag ttgtgagctc gcgcgtagtc ctcggtaata
    12421 tcgcagaaca aaagtacctg atatcgagtg tacttcaagt cacaccggcg agtgtggcac
    12481 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
    12541 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
    12601 cttcccaaca gttgcgcagc ctgaatggcg aatgctagag cagcttgagc ttggatcaga
    12661 ttgtcgtttc ccgccttcag tttaaactat cagtgtttga caggatatat tggcgggtaa
    12721 acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt
    12781 tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta
    12841 ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt
    12901 cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct
    12961 tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga
    13021 accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg
    13081 tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca
    13141 ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga
    13201 tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc
    13261 gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc
    13321 gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg
    13381 tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc
    13441 gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac
    13501 agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg
    13561 cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga
    13621 cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg
    13681 ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg
    13741 ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta
    13801 cgtgttcgag ccgcccgcgc acgtctcaac cgtgcggctg catgaaatcc tggccggttt
    13861 gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg
    13921 ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta
    13981 tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta
    14041 cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg
    14101 caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat
    14161 tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt
    14221 gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag
    14281 gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag
    14341 ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt
    14401 gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg
    14461 cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc
    14521 cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa
    14581 tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca
    14641 aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg
    14701 ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc
    14761 cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt
    14821 acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac
    14881 cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc
    14941 atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg
    15001 ggcggttggc caggcgtaag cggctgggtt gcctgccggc cctgcaatgg cactggaacc
    15061 cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg
    15121 cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac
    15181 gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca
    15241 aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg
    15301 acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca
    15361 gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga
    15421 tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca
    15481 gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc
    15541 gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg
    15601 tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct
    15661 gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc
    15721 gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg
    15781 aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca
    15841 cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg
    15901 gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat
    15961 ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt
    16021 tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg
    16081 cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat
    16141 ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag
    16201 gtcgaaaagg tctctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg
    16261 ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt
    16321 aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta
    16381 ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag
    16441 agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc
    16501 ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag
    16561 ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc
    16621 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
    16681 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
    16741 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
    16801 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
    16861 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
    16921 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    16981 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    17041 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    17101 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    17161 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    17221 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    17281 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    17341 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    17401 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    17461 cgaggtat
//
