LOCUS       sequence-393578-        3670 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MODULE C plasmid compatible with the Voytas Plant Genome Engineering
            Tool kit with an Agrobacterium promoter rolD expressing the TREX2 
            exonuclease..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Miller SS, Dornbusch MR, Farmer AD, Huertas R, Gutierrez-Gonzalez 
            JJ, Young ND, Samac DA, Curtin SJ
  TITLE     Alfalfa (Medicago sativa L.) pho2 mutant plants hyperaccumulate 
            phosphate.
  JOURNAL   G3 (Bethesda). 2022 May 30;12(6). pii: 6574359. doi: 
            10.1093/g3journal/jkac096.
  PUBMED    35471600
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2022-05-30"; volume: "12(6). pii: 6574359. 
            doi"; pages: " 10.1093/g3journal/jkac096"
COMMENT     Sequence Label: pMOD_C2911
FEATURES             Location/Qualifiers
     source          1..3670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1163..1267
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2335..2923
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2824..2843
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3077..3094
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     3150..3166
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggatgtctga gccacctcgg gctgagacct ttgtattcct ggacctagaa gccactgggc
       61 tcccaaacat ggaccctgag attgcagaga tatccctttt tgctgttcac cgctcttccc
      121 tggagaaccc agaacgggat gattctggtt ccttggtgct gccccgtgtt ctggacaagc
      181 tcacactgtg catgtgcccg gagcgcccct ttactgccaa ggccagtgag attactggtt
      241 tgagcagcga aagcctgatg cactgcggga aggctggttt caatggcgct gtggtaagga
      301 cactgcaggg cttcctaagc cgccaggagg gccccatctg ccttgtggcc cacaatggct
      361 tcgattatga cttcccactg ctgtgcacgg agctacaacg tctgggtgcc catctgcccc
      421 aagacactgt ctgcctggac acactgcctg cattgcgggg cctggaccgt gctcacagcc
      481 acggcaccag ggctcaaggc cgcaaaagct acagcctggc cagtctcttc caccgctact
      541 tccaggctga acccagtgct gcccattcag cagaaggtga tgtgcacacc ctgcttctga
      601 tcttcctgca tcgtgctcct gagctgctcg cctgggcaga tgagcaggcc cgcagctggg
      661 ctcatattga gcccatgtac gtgccacctg atggtccaag cctcgaagcc tgataactcg
      721 aggttcgagt attatggcat tgggaaaact gtttttcttg taccatttgt tgtgcttgta
      781 atttactgtg ttttttattc ggttttcgct atcgaactgt gaaatggaaa tggatggaga
      841 agagttaatg aatgatatgg tccttttgtt cattctcaaa ttaatattat ttgttttttc
      901 tcttatttgt tgtgtgttga atttgaaatt ataagagata tgcaaacatt ttgttttgag
      961 taaaaatgtg tcaaatcgtg gcctctaatg accgaagtta atatgaggag taaaacactt
     1021 gtagttgtga gctcgcgcgt agtcctcggt aatatcgcag aacaaaagta cctgatatcg
     1081 agtgtacttc aagtcacacc ggcgagtgac tagcaggtga tgaaaagcct tagggtcagg
     1141 tggcactttt cggggaaatg tgcgatgaac ccctatttgt ttatttttct aaatacattc
     1201 aaatatgtat ccgctaatga gacaataacc ctgataaatg cttcaattat attgaaaaag
     1261 gaagagtatg agtatccaac atttccgtgt cgcccttatt cccttttttg cggccttctg
     1321 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     1381 gggtgcccga gtgggttaca ttgaactgga tctcaacagc ggcaaaatct tagagagttt
     1441 tcgccccgaa gaacgcttcc caatgatgag cactttcaaa gttctgctat gtggcgcggt
     1501 gttatcccgt attgatgctg ggcaagagca actcggtcgc cgcatacact attctcagaa
     1561 tgacttggtt gagtattcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     1621 agaattatgt agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     1681 aacgattgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     1741 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     1801 cacgatgcct gtagcaatgg caacaactct ccgcaaacta ttaactggcg aactacttac
     1861 tcttgcttca cgccagcaac tcattgactg gatggaggcg gacaaagttg caggaccact
     1921 tctgcgctcg gcacttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     1981 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     2041 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     2101 aggtgcctca ctgattaagc attggtaact gtcagacgaa gggaaataaa tagtagcccg
     2161 cctgatgtgc gggctttttt ttggtggtgg ttaccagtgt cgcttagcta ccgtaggtcc
     2221 tcttaggcgg tccgattagc gacatagtca tcaatgcctt gacggcaaaa tcccttaacg
     2281 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     2341 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     2401 ggtttgtttg ccggatcaag agctactaac tctttttccg aaggtaactg gcttcagcag
     2461 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     2521 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     2581 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     2641 gcggtcgggc tgaacggggg gttcgtgctc acagcccagc ttggagcgaa cgacctacac
     2701 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2761 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2821 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     2881 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     2941 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     3001 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     3061 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc cacctgcaac
     3121 gccggcgtaa tatggcgcgc cgaagagcgc gaggtcgacg gtatcgataa gcttgatatc
     3181 gaattcctgc agcccggggg atccgcccgc tctagactgc cttgtaggtg cgaattttct
     3241 ctgctcaaat tgttgaggtt agcggatttg taaacgcgtt tatatgggct gcttggaggg
     3301 tacttttgga ttaatttttt tctgccagcg cattctgacg cggcaccgct ttggaaagtg
     3361 cgctgtgggt ccgcgttttc tacaataatg tgccgatccg gtcagaaagt atatggatga
     3421 gttgtgccag cctcaccaac gtgctgcagg cccatcatga ctacttcaat gttaatgggg
     3481 gtaatgaata aataggcgaa attgggttca cggtgggccc agggaatata atattgccgc
     3541 agaggtagtc ggatgccaag gcccgcaact aatagttcac gaacaaattc attgtagtgg
     3601 gcggccaact ccaaaaccaa ttgccagtta ttgtattgca atacatatat ggcttcctcc
     3661 cttgcctgca
//
