LOCUS       sequence-393613-       13572 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  induction of phase-separated condensate by CRY2Clust.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13572)
  AUTHORS   Kim YJ, Lee M Jr, Lee YT, Jing J, Sanders JT, Botten GA, He L, Lyu 
            J, Zhang Y, Mettlen M, Ly P, Zhou Y, Xu J
  TITLE     Light-activated macromolecular phase separation modulates 
            transcription by reconfiguring chromatin interactions.
  JOURNAL   Sci Adv. 2023 Mar 31;9(13):eadg1123. doi: 10.1126/sciadv.adg1123. 
            Epub 2023 Mar 31.
  PUBMED    37000871
REFERENCE   2  (bases 1 to 13572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-03-31"; volume: "9(13):eadg1123. doi"; pages: " 
            10.1126/sciadv.adg1123. Epub 2023 Mar 31"
COMMENT     Sequence Label: dCas9-CRY2Clust
FEATURES             Location/Qualifiers
     source          1..13572
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             102..735
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    782..907
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1404..1637
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1822..1866
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2129..2244
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2284..2587
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2588..2791
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2742..2762
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             2826..3533
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2971..2989)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3239..3258
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(5113..5130)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5163..5207
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             5238..5258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5259..5282,5283..5285,5286..7772,7773..7775,
                     7776..9359)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9369..9389
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    9642..10230
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9695..9715)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10113..10124)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10437..11070
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11198..11214)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11198..11214)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11211..11233)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11222..11238
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11246..11252,11253..11270,11271..11276))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11291..11312
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11429..11446)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11600..12188)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11680..11699)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12359..13150,13151..13219))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12982..13001
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13220..13324)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    13372..13506
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13409..13428)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13463..13482
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
       61 agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc
      121 ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
      181 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
      241 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
      301 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
      361 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
      421 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
      481 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
      541 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
      601 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
      661 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
      721 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
      781 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
      841 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
      901 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
      961 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     1021 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     1081 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     1141 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     1201 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc
     1261 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     1321 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     1381 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     1441 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     1501 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     1561 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     1621 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     1681 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     1741 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     1801 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     1861 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     1921 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     1981 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     2041 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     2101 ttagtgaacg gatctcgacg gtatcgcctt taaaagaaaa ggggggattg gggggtacag
     2161 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     2221 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagttta
     2281 tcgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     2341 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     2401 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     2461 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     2521 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     2581 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     2641 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     2701 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     2761 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg
     2821 gtggaatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
     2881 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
     2941 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
     3001 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
     3061 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
     3121 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
     3181 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
     3241 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
     3301 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
     3361 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
     3421 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     3481 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagggtagcg
     3541 gaccaccggt cgccaccatg aagatggaca aaaagaccat cgtctggttt cggagagatt
     3601 tgagaataga agataatccc gcgctcgccg ccgcggccca cgagggttcc gtcttccccg
     3661 ttttcatttg gtgtcctgaa gaagaaggcc agttttatcc cggaagggcc tctaggtggt
     3721 ggatgaagca aagtctggcc catcttagcc agtcactgaa agcactgggc agtgatctta
     3781 ccctgatcaa gacacacaat accatctctg ccatcctcga ctgcatcagg gtgaccggcg
     3841 caacgaaagt cgtgtttaac cacctgtacg atccagttag tctggtgcgc gaccacactg
     3901 tgaaggagaa gctggtggaa cgggggatca gtgtgcagag ctacaacggg gaccttctgt
     3961 acgagccatg ggagatctat tgcgagaaag ggaaaccgtt cacctccttc aacagttact
     4021 ggaagaaatg tttggatatg tcaatagagt ccgttatgtt gccccctccc tggagactga
     4081 tgccgattac tgctgctgca gaggccatct gggcctgctc catcgaggaa ctcggtctgg
     4141 aaaatgaagc agaaaagcca agcaatgcac ttctcactag agcctggagc cccggctggt
     4201 ctaatgccga caagctgctt aacgagttca tcgaaaaaca actgattgac tacgcgaaga
     4261 actccaagaa agtggtaggt aactcaacta gcttgctctc tccatatctc cattttggcg
     4321 agatttctgt ccgccatgta tttcagtgcg ctcggatgaa acagattatc tgggctcgcg
     4381 ataaaaacag cgaaggcgaa gaaagcgccg atctgttcct gcgagggatc ggacttcggg
     4441 aatactcccg gtatatatgt ttcaactttc cattcacaca cgagcagagt ctgttgtccc
     4501 acctcaggtt cttcccctgg gacgccgatg tcgacaaatt caaggcatgg agacagggaa
     4561 ggacagggta cccactcgtg gatgctggca tgagagagct ctgggctaca ggctggatgc
     4621 acaaccgcat ccgggtaatc gtgtcctcat ttgctgtcaa gtttctgctc ctgccttgga
     4681 aatggggaat gaagtacttt tgggataccc ttctcgacgc cgacttggag tgtgacattc
     4741 tgggatggca atatattagc gggtcaattc ctgacggcca tgagttggac aggttggaca
     4801 atccggcctt gcagggagct aagtatgatc ccgaaggaga gtatattcga cagtggctcc
     4861 ccgagctggc ccgacttcct acggagtgga ttcaccatcc ttgggacgca ccactgacag
     4921 tgctcaaggc aagcggggtg gagctgggca ccaattacgc taagcctata gttgatatag
     4981 atacagcacg cgagctgctg gctaaagcga tctctcgcac tcgggaggcg cagattatga
     5041 tcggtgctgc cgcaagggac cctcctgatc ttgacaatgt gtacagtggg ggaggcggag
     5101 ggggtggttc aggtggtggt ggtggtggtg gttcgggtgg tggcggaggg ggaggctccg
     5161 ggggcctgaa cgacatcttc gaggctcaga aaatcgaatg gcacgaaggc gcgccgagct
     5221 cgaggatcct tgctagcccc aaaaagaaga ggaaagtgga caagaagtat tctatcggac
     5281 tggccatcgg gactaatagc gtcgggtggg ccgtgatcac tgacgagtac aaggtgccct
     5341 ctaagaagtt caaggtgctc gggaacaccg accggcattc catcaagaaa aatctgatcg
     5401 gagctctcct ctttgattca ggggagaccg ctgaagcaac ccgcctcaag cggactgcta
     5461 gacggcggta caccaggagg aagaaccgga tttgttacct tcaagagata ttctccaacg
     5521 aaatggcaaa ggtcgacgac agcttcttcc ataggctgga agaatcattc ctcgtggaag
     5581 aggataagaa gcatgaacgg catcccatct tcggtaatat cgtcgacgag gtggcctatc
     5641 acgagaaata cccaaccatc taccatcttc gcaaaaagct ggtggactca accgacaagg
     5701 cagacctccg gcttatctac ctggccctgg cccacatgat caagttcaga ggccacttcc
     5761 tgatcgaggg cgacctcaat cctgacaata gcgatgtgga taaactgttc atccagctgg
     5821 tgcagactta caaccagctc tttgaagaga accccatcaa tgcaagcgga gtcgatgcca
     5881 aggccattct gtcagcccgg ctgtcaaaga gccgcagact tgagaatctt atcgctcagc
     5941 tgccgggtga aaagaaaaat ggactgttcg ggaacctgat tgctctttca cttgggctga
     6001 ctcccaattt caagtctaat ttcgacctgg cagaggatgc caagctgcaa ctgtccaagg
     6061 acacctatga tgacgatctc gacaacctcc tggcccagat cggtgaccaa tacgccgacc
     6121 ttttccttgc tgctaagaat ctttctgacg ccatcctgct gtctgacatt ctccgcgtga
     6181 acactgaaat caccaaggcc cctctttcag cttcaatgat taagcggtat gatgagcacc
     6241 accaggacct gaccctgctt aaggcactcg tccggcagca gcttccggag aagtacaagg
     6301 aaatcttctt tgaccagtca aagaatggat acgccggcta catcgacgga ggtgcctccc
     6361 aagaggaatt ttataagttt atcaaaccta tccttgagaa gatggacggc accgaagagc
     6421 tcctcgtgaa actgaatcgg gaggatctgc tgcggaagca gcgcactttc gacaatggga
     6481 gcattcccca ccagatccat cttggggagc ttcacgccat ccttcggcgc caagaggact
     6541 tctacccctt tcttaaggac aacagggaga agattgagaa aattctcact ttccgcatcc
     6601 cctactacgt gggacccctc gccagaggaa atagccggtt tgcttggatg accagaaagt
     6661 cagaagaaac tatcactccc tggaacttcg aagaggtggt ggacaaggga gccagcgctc
     6721 agtcattcat cgaacggatg actaacttcg ataagaacct ccccaatgag aaggtcctgc
     6781 cgaaacattc cctgctctac gagtacttta ccgtgtacaa cgagctgacc aaggtgaaat
     6841 atgtcaccga agggatgagg aagcccgcat tcctgtcagg cgaacaaaag aaggcaattg
     6901 tggaccttct gttcaagacc aatagaaagg tgaccgtgaa gcagctgaag gaggactatt
     6961 tcaagaaaat tgaatgcttc gactctgtgg agattagcgg ggtcgaagat cggttcaacg
     7021 caagcctggg tacctaccat gatctgctta agatcatcaa ggacaaggat tttctggaca
     7081 atgaggagaa cgaggacatc cttgaggaca ttgtcctgac tctcactctg ttcgaggacc
     7141 gggaaatgat cgaggagagg cttaagacct acgcccatct gttcgacgat aaagtgatga
     7201 agcaacttaa acggagaaga tataccggat ggggacgcct tagccgcaaa ctcatcaacg
     7261 gaatccggga caaacagagc ggaaagacca ttcttgattt ccttaagagc gacggattcg
     7321 ctaatcgcaa cttcatgcaa cttatccatg atgattccct gacctttaag gaggacatcc
     7381 agaaggccca agtgtctgga caaggtgact cactgcacga gcatatcgca aatctggctg
     7441 gttcacccgc tattaagaag ggtattctcc agaccgtgaa agtcgtggac gagctggtca
     7501 aggtgatggg tcgccataaa ccagagaaca ttgtcatcga gatggccagg gaaaaccaga
     7561 ctacccagaa gggacagaag aacagcaggg agcggatgaa aagaattgag gaagggatta
     7621 aggagctcgg gtcacagatc cttaaagagc acccggtgga aaacacccag cttcagaatg
     7681 agaagctcta tctgtactac cttcaaaatg gacgcgatat gtatgtggac caagagcttg
     7741 atatcaacag gctctcagac tacgacgtgg acgccatcgt ccctcagagc ttcctcaaag
     7801 acgactcaat tgacaataag gtgctgactc gctcagacaa gaaccgggga aagtcagata
     7861 acgtgccctc agaggaagtc gtgaaaaaga tgaagaacta ttggcgccag cttctgaacg
     7921 caaagctgat cactcagcgg aagttcgaca atctcactaa ggctgagagg ggcggactga
     7981 gcgaactgga caaagcagga ttcattaaac ggcaacttgt ggagactcgg cagattacta
     8041 aacatgtcgc ccaaatcctt gactcacgca tgaataccaa gtacgacgaa aacgacaaac
     8101 ttatccgcga ggtgaaggtg attaccctga agtccaagct ggtcagcgat ttcagaaagg
     8161 actttcaatt ctacaaagtg cgggagatca ataactatca tcatgctcat gacgcatatc
     8221 tgaatgccgt ggtgggaacc gccctgatca agaagtaccc aaagctggaa agcgagttcg
     8281 tgtacggaga ctacaaggtc tacgacgtgc gcaagatgat tgccaaatct gagcaggaga
     8341 tcggaaaggc caccgcaaag tacttcttct acagcaacat catgaatttc ttcaagaccg
     8401 aaatcaccct tgcaaacggt gagatccgga agaggccgct catcgagact aatggggaga
     8461 ctggcgaaat cgtgtgggac aagggcagag atttcgctac cgtgcgcaaa gtgctttcta
     8521 tgcctcaagt gaacatcgtg aagaaaaccg aggtgcaaac cggaggcttt tctaaggaat
     8581 caatcctccc caagcgcaac tccgacaagc tcattgcaag gaagaaggat tgggacccta
     8641 agaagtacgg cggattcgat tcaccaactg tggcttattc tgtcctggtc gtggctaagg
     8701 tggaaaaagg aaagtctaag aagctcaaga gcgtgaagga actgctgggt atcaccatta
     8761 tggagcgcag ctccttcgag aagaacccaa ttgactttct cgaagccaaa ggttacaagg
     8821 aagtcaagaa ggaccttatc atcaagctcc caaagtatag cctgttcgaa ctggagaatg
     8881 ggcggaagcg gatgctcgcc tccgctggcg aacttcagaa gggtaatgag ctggctctcc
     8941 cctccaagta cgtgaatttc ctctaccttg caagccatta cgagaagctg aaggggagcc
     9001 ccgaggacaa cgagcaaaag caactgtttg tggagcagca taagcattat ctggacgaga
     9061 tcattgagca gatttccgag ttttctaaac gcgtcattct cgctgatgcc aacctcgata
     9121 aagtccttag cgcatacaat aagcacagag acaaaccaat tcgggagcag gctgagaata
     9181 tcatccacct gttcaccctc accaatcttg gtgcccctgc cgcattcaag tacttcgaca
     9241 ccaccatcga ccggaaacgc tatacctcca ccaaagaagt gctggacgcc accctcatcc
     9301 accagagcat caccggactt tacgaaactc ggattgacct ctcacagctc ggaggggatg
     9361 agggagctcc caagaaaaag cgcaaggtag gtagttccgc cggaaaggcc ggtgaaggtg
     9421 aaatccctgc ccctcttgct ggtaccgttt ctaagatact ggtaaaagaa ggtgacactg
     9481 ttaaagctgg tcaaacagtt ctggtgctgg aggctatgaa aatggagaca gaaattaacg
     9541 ctcctactga cggaaaagtt gaaaaggtgt tagttaagga aagagatgct gttcaaggtg
     9601 gtcaaggtct aatcaagatc ggcgtttagc gcgtctggaa caatcaacct ctggattaca
     9661 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     9721 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     9781 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     9841 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     9901 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     9961 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
    10021 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
    10081 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
    10141 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
    10201 gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga
    10261 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct
    10321 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc
    10381 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga
    10441 agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca
    10501 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
    10561 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
    10621 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
    10681 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
    10741 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
    10801 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
    10861 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
    10921 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
    10981 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
    11041 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
    11101 atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt
    11161 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
    11221 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
    11281 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
    11341 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    11401 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
    11461 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    11521 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    11581 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    11641 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    11701 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    11761 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    11821 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    11881 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    11941 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    12001 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
    12061 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    12121 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
    12181 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
    12241 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
    12301 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
    12361 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
    12421 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
    12481 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
    12541 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
    12601 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
    12661 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
    12721 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
    12781 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    12841 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    12901 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    12961 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    13021 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    13081 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    13141 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    13201 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    13261 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    13321 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt
    13381 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
    13441 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
    13501 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct
    13561 attaccactg cc
//
