LOCUS       sequence-393616-       14234 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  induction of phase-separated condensate mediated by FUSN-IDR mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14234)
  AUTHORS   Kim YJ, Lee M Jr, Lee YT, Jing J, Sanders JT, Botten GA, He L, Lyu 
            J, Zhang Y, Mettlen M, Ly P, Zhou Y, Xu J
  TITLE     Light-activated macromolecular phase separation modulates 
            transcription by reconfiguring chromatin interactions.
  JOURNAL   Sci Adv. 2023 Mar 31;9(13):eadg1123. doi: 10.1126/sciadv.adg1123. 
            Epub 2023 Mar 31.
  PUBMED    37000871
REFERENCE   2  (bases 1 to 14234)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-03-31"; volume: "9(13):eadg1123. doi"; pages: " 
            10.1126/sciadv.adg1123. Epub 2023 Mar 31"
COMMENT     Sequence Label: dCas9-CRY2Clust-FUSN-mut
FEATURES             Location/Qualifiers
     source          1..14234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2183..2486
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2487..2690
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2641..2661
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             3387..4094
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3532..3550)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3800..3819
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(5674..5691)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5724..5768
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             5799..5819
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(5820..5843,5844..5846,5847..8333,8334..8336,
                     8337..9920)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             9930..9950
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    10203..10791
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10256..10276)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10674..10685)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10998..11631
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11759..11775)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11759..11775)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11772..11794)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11783..11799
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11807..11813,11814..11831,11832..11837))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11852..11873
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11990..12007)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12161..12749)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12241..12260)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12920..13711,13712..13780))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13543..13562
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13781..13885)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    13933..14067
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13970..13989)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14024..14043
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgcgttacat aacttacggt aaatggcccg cctggctgac
     2221 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     2281 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     2341 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     2401 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     2461 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     2521 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     2581 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     2641 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga
     2701 accgtcagat ccgctagggg cccatggcct caaacgatta tacccaacaa gcaacccaaa
     2761 gctctggggc ctctcccacc cagcccgggc agggctattc ccagcagagc agtcagccct
     2821 ctggacagca gagttctagt ggttctagcc agtccacgga cacttcaggc tatggccaga
     2881 gcagctcttc ttcttctggc cagagccaga acacaggcta tggaactcag tcaactcccc
     2941 agggatatgg ctcgactggc ggctatggca gtagccagag ctcccaatcg tcttctgggc
     3001 agcagtcctc ctctcctggc tctggccagc agccagctcc cagcagcacc tcgggaagtt
     3061 acggtagcag ttctcagagc agcagctatg ggcagcccca gagtgggagc tctagccagc
     3121 agcctagcta tggtggacag cagcaaagct ctggacagca gcaaagctct aatccccctc
     3181 agggctctgg acagcagaac cagtctaaca gcagcagtgg tggtggaggt ggaggtggag
     3241 gtggaggtaa ctatggccaa gatcaatcct ccatgagtag tggtggtggc agtggtggcg
     3301 gttatggcaa tcaagaccag agtggtggag gtggcagcgg tggctatgga cagcaggacc
     3361 gtggaggtgg aggaactagt gctagcatgg tgagcaaggg cgaggaggat aacatggcca
     3421 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
     3481 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     3541 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
     3601 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
     3661 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     3721 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
     3781 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
     3841 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
     3901 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
     3961 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
     4021 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
     4081 acgagctgta caagggtagc ggaccaccgg tcgccaccat gaagatggac aaaaagacca
     4141 tcgtctggtt tcggagagat ttgagaatag aagataatcc cgcgctcgcc gccgcggccc
     4201 acgagggttc cgtcttcccc gttttcattt ggtgtcctga agaagaaggc cagttttatc
     4261 ccggaagggc ctctaggtgg tggatgaagc aaagtctggc ccatcttagc cagtcactga
     4321 aagcactggg cagtgatctt accctgatca agacacacaa taccatctct gccatcctcg
     4381 actgcatcag ggtgaccggc gcaacgaaag tcgtgtttaa ccacctgtac gatccagtta
     4441 gtctggtgcg cgaccacact gtgaaggaga agctggtgga acgggggatc agtgtgcaga
     4501 gctacaacgg ggaccttctg tacgagccat gggagatcta ttgcgagaaa gggaaaccgt
     4561 tcacctcctt caacagttac tggaagaaat gtttggatat gtcaatagag tccgttatgt
     4621 tgccccctcc ctggagactg atgccgatta ctgctgctgc agaggccatc tgggcctgct
     4681 ccatcgagga actcggtctg gaaaatgaag cagaaaagcc aagcaatgca cttctcacta
     4741 gagcctggag ccccggctgg tctaatgccg acaagctgct taacgagttc atcgaaaaac
     4801 aactgattga ctacgcgaag aactccaaga aagtggtagg taactcaact agcttgctct
     4861 ctccatatct ccattttggc gagatttctg tccgccatgt atttcagtgc gctcggatga
     4921 aacagattat ctgggctcgc gataaaaaca gcgaaggcga agaaagcgcc gatctgttcc
     4981 tgcgagggat cggacttcgg gaatactccc ggtatatatg tttcaacttt ccattcacac
     5041 acgagcagag tctgttgtcc cacctcaggt tcttcccctg ggacgccgat gtcgacaaat
     5101 tcaaggcatg gagacaggga aggacagggt acccactcgt ggatgctggc atgagagagc
     5161 tctgggctac aggctggatg cacaaccgca tccgggtaat cgtgtcctca tttgctgtca
     5221 agtttctgct cctgccttgg aaatggggaa tgaagtactt ttgggatacc cttctcgacg
     5281 ccgacttgga gtgtgacatt ctgggatggc aatatattag cgggtcaatt cctgacggcc
     5341 atgagttgga caggttggac aatccggcct tgcagggagc taagtatgat cccgaaggag
     5401 agtatattcg acagtggctc cccgagctgg cccgacttcc tacggagtgg attcaccatc
     5461 cttgggacgc accactgaca gtgctcaagg caagcggggt ggagctgggc accaattacg
     5521 ctaagcctat agttgatata gatacagcac gcgagctgct ggctaaagcg atctctcgca
     5581 ctcgggaggc gcagattatg atcggtgctg ccgcaaggga ccctcctgat cttgacaatg
     5641 tgtacagtgg gggaggcgga gggggtggtt caggtggtgg tggtggtggt ggttcgggtg
     5701 gtggcggagg gggaggctcc gggggcctga acgacatctt cgaggctcag aaaatcgaat
     5761 ggcacgaagg cgcgccgagc tcgaggatcc ttgctagccc caaaaagaag aggaaagtgg
     5821 acaagaagta ttctatcgga ctggccatcg ggactaatag cgtcgggtgg gccgtgatca
     5881 ctgacgagta caaggtgccc tctaagaagt tcaaggtgct cgggaacacc gaccggcatt
     5941 ccatcaagaa aaatctgatc ggagctctcc tctttgattc aggggagacc gctgaagcaa
     6001 cccgcctcaa gcggactgct agacggcggt acaccaggag gaagaaccgg atttgttacc
     6061 ttcaagagat attctccaac gaaatggcaa aggtcgacga cagcttcttc cataggctgg
     6121 aagaatcatt cctcgtggaa gaggataaga agcatgaacg gcatcccatc ttcggtaata
     6181 tcgtcgacga ggtggcctat cacgagaaat acccaaccat ctaccatctt cgcaaaaagc
     6241 tggtggactc aaccgacaag gcagacctcc ggcttatcta cctggccctg gcccacatga
     6301 tcaagttcag aggccacttc ctgatcgagg gcgacctcaa tcctgacaat agcgatgtgg
     6361 ataaactgtt catccagctg gtgcagactt acaaccagct ctttgaagag aaccccatca
     6421 atgcaagcgg agtcgatgcc aaggccattc tgtcagcccg gctgtcaaag agccgcagac
     6481 ttgagaatct tatcgctcag ctgccgggtg aaaagaaaaa tggactgttc gggaacctga
     6541 ttgctctttc acttgggctg actcccaatt tcaagtctaa tttcgacctg gcagaggatg
     6601 ccaagctgca actgtccaag gacacctatg atgacgatct cgacaacctc ctggcccaga
     6661 tcggtgacca atacgccgac cttttccttg ctgctaagaa tctttctgac gccatcctgc
     6721 tgtctgacat tctccgcgtg aacactgaaa tcaccaaggc ccctctttca gcttcaatga
     6781 ttaagcggta tgatgagcac caccaggacc tgaccctgct taaggcactc gtccggcagc
     6841 agcttccgga gaagtacaag gaaatcttct ttgaccagtc aaagaatgga tacgccggct
     6901 acatcgacgg aggtgcctcc caagaggaat tttataagtt tatcaaacct atccttgaga
     6961 agatggacgg caccgaagag ctcctcgtga aactgaatcg ggaggatctg ctgcggaagc
     7021 agcgcacttt cgacaatggg agcattcccc accagatcca tcttggggag cttcacgcca
     7081 tccttcggcg ccaagaggac ttctacccct ttcttaagga caacagggag aagattgaga
     7141 aaattctcac tttccgcatc ccctactacg tgggacccct cgccagagga aatagccggt
     7201 ttgcttggat gaccagaaag tcagaagaaa ctatcactcc ctggaacttc gaagaggtgg
     7261 tggacaaggg agccagcgct cagtcattca tcgaacggat gactaacttc gataagaacc
     7321 tccccaatga gaaggtcctg ccgaaacatt ccctgctcta cgagtacttt accgtgtaca
     7381 acgagctgac caaggtgaaa tatgtcaccg aagggatgag gaagcccgca ttcctgtcag
     7441 gcgaacaaaa gaaggcaatt gtggaccttc tgttcaagac caatagaaag gtgaccgtga
     7501 agcagctgaa ggaggactat ttcaagaaaa ttgaatgctt cgactctgtg gagattagcg
     7561 gggtcgaaga tcggttcaac gcaagcctgg gtacctacca tgatctgctt aagatcatca
     7621 aggacaagga ttttctggac aatgaggaga acgaggacat ccttgaggac attgtcctga
     7681 ctctcactct gttcgaggac cgggaaatga tcgaggagag gcttaagacc tacgcccatc
     7741 tgttcgacga taaagtgatg aagcaactta aacggagaag atataccgga tggggacgcc
     7801 ttagccgcaa actcatcaac ggaatccggg acaaacagag cggaaagacc attcttgatt
     7861 tccttaagag cgacggattc gctaatcgca acttcatgca acttatccat gatgattccc
     7921 tgacctttaa ggaggacatc cagaaggccc aagtgtctgg acaaggtgac tcactgcacg
     7981 agcatatcgc aaatctggct ggttcacccg ctattaagaa gggtattctc cagaccgtga
     8041 aagtcgtgga cgagctggtc aaggtgatgg gtcgccataa accagagaac attgtcatcg
     8101 agatggccag ggaaaaccag actacccaga agggacagaa gaacagcagg gagcggatga
     8161 aaagaattga ggaagggatt aaggagctcg ggtcacagat ccttaaagag cacccggtgg
     8221 aaaacaccca gcttcagaat gagaagctct atctgtacta ccttcaaaat ggacgcgata
     8281 tgtatgtgga ccaagagctt gatatcaaca ggctctcaga ctacgacgtg gacgccatcg
     8341 tccctcagag cttcctcaaa gacgactcaa ttgacaataa ggtgctgact cgctcagaca
     8401 agaaccgggg aaagtcagat aacgtgccct cagaggaagt cgtgaaaaag atgaagaact
     8461 attggcgcca gcttctgaac gcaaagctga tcactcagcg gaagttcgac aatctcacta
     8521 aggctgagag gggcggactg agcgaactgg acaaagcagg attcattaaa cggcaacttg
     8581 tggagactcg gcagattact aaacatgtcg cccaaatcct tgactcacgc atgaatacca
     8641 agtacgacga aaacgacaaa cttatccgcg aggtgaaggt gattaccctg aagtccaagc
     8701 tggtcagcga tttcagaaag gactttcaat tctacaaagt gcgggagatc aataactatc
     8761 atcatgctca tgacgcatat ctgaatgccg tggtgggaac cgccctgatc aagaagtacc
     8821 caaagctgga aagcgagttc gtgtacggag actacaaggt ctacgacgtg cgcaagatga
     8881 ttgccaaatc tgagcaggag atcggaaagg ccaccgcaaa gtacttcttc tacagcaaca
     8941 tcatgaattt cttcaagacc gaaatcaccc ttgcaaacgg tgagatccgg aagaggccgc
     9001 tcatcgagac taatggggag actggcgaaa tcgtgtggga caagggcaga gatttcgcta
     9061 ccgtgcgcaa agtgctttct atgcctcaag tgaacatcgt gaagaaaacc gaggtgcaaa
     9121 ccggaggctt ttctaaggaa tcaatcctcc ccaagcgcaa ctccgacaag ctcattgcaa
     9181 ggaagaagga ttgggaccct aagaagtacg gcggattcga ttcaccaact gtggcttatt
     9241 ctgtcctggt cgtggctaag gtggaaaaag gaaagtctaa gaagctcaag agcgtgaagg
     9301 aactgctggg tatcaccatt atggagcgca gctccttcga gaagaaccca attgactttc
     9361 tcgaagccaa aggttacaag gaagtcaaga aggaccttat catcaagctc ccaaagtata
     9421 gcctgttcga actggagaat gggcggaagc ggatgctcgc ctccgctggc gaacttcaga
     9481 agggtaatga gctggctctc ccctccaagt acgtgaattt cctctacctt gcaagccatt
     9541 acgagaagct gaaggggagc cccgaggaca acgagcaaaa gcaactgttt gtggagcagc
     9601 ataagcatta tctggacgag atcattgagc agatttccga gttttctaaa cgcgtcattc
     9661 tcgctgatgc caacctcgat aaagtcctta gcgcatacaa taagcacaga gacaaaccaa
     9721 ttcgggagca ggctgagaat atcatccacc tgttcaccct caccaatctt ggtgcccctg
     9781 ccgcattcaa gtacttcgac accaccatcg accggaaacg ctatacctcc accaaagaag
     9841 tgctggacgc caccctcatc caccagagca tcaccggact ttacgaaact cggattgacc
     9901 tctcacagct cggaggggat gagggagctc ccaagaaaaa gcgcaaggta ggtagttccg
     9961 ccggaaaggc cggtgaaggt gaaatccctg cccctcttgc tggtaccgtt tctaagatac
    10021 tggtaaaaga aggtgacact gttaaagctg gtcaaacagt tctggtgctg gaggctatga
    10081 aaatggagac agaaattaac gctcctactg acggaaaagt tgaaaaggtg ttagttaagg
    10141 aaagagatgc tgttcaaggt ggtcaaggtc taatcaagat cggcgtttag cgcgtctgga
    10201 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
    10261 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
    10321 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
    10381 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
    10441 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
    10501 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
    10561 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
    10621 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
    10681 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
    10741 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctggaatta
    10801 attctgcagt cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
    10861 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
    10921 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
    10981 aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
    11041 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc
    11101 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga
    11161 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca
    11221 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt
    11281 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg
    11341 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga
    11401 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct
    11461 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
    11521 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
    11581 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
    11641 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
    11701 ggccttgaca ttgctagcgt ttaccgtcga cctctagcta gagcttggcg taatcatggt
    11761 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
    11821 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
    11881 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
    11941 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
    12001 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    12061 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    12121 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    12181 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    12241 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    12301 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    12361 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    12421 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    12481 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    12541 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    12601 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    12661 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    12721 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    12781 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    12841 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    12901 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    12961 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    13021 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    13081 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    13141 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    13201 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    13261 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    13321 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    13381 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    13441 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    13501 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    13561 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    13621 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    13681 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    13741 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    13801 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    13861 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
    13921 gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    13981 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    14041 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
    14101 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
    14161 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
    14221 acaaacttag tagt
//
