LOCUS       sequence-393627-       11716 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter 
            drives expression of activated beta-catenin preceded by a blue 
            fluorescent protein (BFP)-STOP cassette flanked by loxP sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11716)
  AUTHORS   Kalasekar SM, Kotiyal S, Conley C, Phan C, Young A, Evason KJ
  TITLE     Heterogeneous beta-catenin activation is sufficient to cause 
            hepatocellular carcinoma in zebrafish.
  JOURNAL   Biol Open. 2019 Oct 17;8(10):bio047829. doi: 10.1242/bio.047829.
  PUBMED    31575545
REFERENCE   2  (bases 1 to 11716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Open."; date: "2019-10-17"; volume: "8(10):bio047829. doi"; pages: "
            10.1242/bio.047829"
COMMENT     Sequence Label: pKE12-ISceI-fabp10a:loxP-BFP-loxP-Xla.Ctnnb1
FEATURES             Location/Qualifiers
     source          1..11716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     26..45
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        26..44
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(2952..2985)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(3009..3011,3012..3014,3015..3728)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(3054..3075)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     intron          3812..3877
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4007..4027
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4452..4586
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(4641..4674)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4769..4804
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
     polyA_signal    7191..7312
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    7355..7562
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     7551..7568
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(7586..8296)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7864..7883)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8133..8151
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(8880..8900)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8880..8898)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8931..8948)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8931..8947)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8940..8962)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(9088..9543)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9225..9246)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9437..9456
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        9570..9674
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9675..9743,9744..10535)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9893..9912)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10706..11294
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11195..11214
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11448..11465
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11582..11603
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11618..11623,11624..11641,11642..11648)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11656..11672
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11661..11683
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11680..11696
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11680..11696
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tagggataac agggtaatgc gcgcgtaata cgactcacta tagggcgaat tggaattcct
       61 gagcatcaga atggggaagg aagaggattt cagtgacttt gaacgaggca tggttgttgc
      121 tgccagatgg gctgctctga gtatttcaga aactgctgat cttcagggat tttcacgcac
      181 aaccatctct agggtttaca gagaatgatc tgaaaagagg aaatatccag tgagcggcag
      241 ttctgtgggt gcaaatgcct tgttgatgcc agagatcaga ggagaatggc cagactggtt
      301 ccagctgata gaaaggcaac agtaactcaa ataagcactc gttacaaccg agctctgcag
      361 aagagcatct ctgaacacac aacacgtcca accttgaggc agatgggcta cagcagcaga
      421 agaccacacc gggtgccgct cctgtcagct aagaacagga aactgaggct acaattcaca
      481 cagactcacc aaaactggac aatatattat tagcccccct tagaattttt catttgataa
      541 tatttttctt ctggcgaaag cctcatttgt tttatatatt atagaataaa attagttttt
      601 aatagttttt atgccatttt aaggtcaata ttattagccc ctttaagcta ttttttttcg
      661 atagtctaca gaacaaacca tcggtataca atgacttgcc taattaccct aacctgccta
      721 gttaccctaa ttaacctagt taagccttta aatgtcactt taagctgtat agaagtgtct
      781 tgaagaatat ctagtctaat attattgact gtcatcatgg caaagataaa ataaatcagt
      841 tattaaaact attatgatta gaaatgtgct gaaacaatct gctctccgat aaacagaaat
      901 tgaacaaaat aaacaggggg gctaataaat ttaaggggtt aaataattct gattgcaaaa
      961 aaaatgatgt ctgcaaaact gttgcaaata atttatttgt gttgatttta agcaaacaaa
     1021 ttaaatttaa taaaatacaa cttaatctgt ttgtttaaat tcagccctaa taaattgttt
     1081 acagccactt aacgtaaaaa aattgagtaa atccaaggaa tcatctctga ataatttttt
     1141 tcagtgtata tatatatata tatattctta caaaacaact catttacttt agtttaattt
     1201 tcaggggcaa aaaactaaag taatcgacgt tgcttgaata aaaagtgtaa ttaagggaat
     1261 gaggtaacat ttaaccatgt gtcaatgcag tttaaatatg ccagttagtg gtatatgttt
     1321 aaatggtaag ctattcaaaa ctttaaacta acttaaccag ccttttgttg tcagactgaa
     1381 cagactttcc atctgcatta ttagagacta atctttggct ggatgaatga ttcatctgct
     1441 gatatttcag aatagacaga ttgaggctgt ttctaatatg attatgcaac ctgagggtga
     1501 ttatttgaag caaactccac agaccagcag gtcattgacc gtcgtgtgtt caaacagagc
     1561 agaaacattt gcaaaactgg tctgacagga gaatccagtc cagcacaaca catatgctga
     1621 gcaaactgaa tcaatcctgc aggtcaactc tcgtgcttta agtttattaa gattatttta
     1681 tttatttatt attttattta tctatttatt tatttagttg tttatttagc aattatttgt
     1741 ttatttattt gtttatttat tcctgcagat catgccttgt gcctttttac atttaattta
     1801 atttttaatt taatttcctt ttattttttt ttattttttt attttatttt attttacagt
     1861 ctgacaaata ctgaactaaa aaccctcaga tcatgtctta tgcatttcat tttattttat
     1921 ttcattttat attattaatt ttaatatttt tattttacag tctgacaaat actgaattaa
     1981 aaaccatcag atcatgtctc atgcatttaa cttaacttta tttaattcaa ttaaattgtt
     2041 tgtttgtttg tttccttgca tttgtttgtt tgttttttac aatctgacat actggaccga
     2101 aaaaactcag atcatgtctt atgcatttta ttttatttta ttttttattt tattgtctat
     2161 tttattttat ttcatcttat tttattttac agtctgacat atggaccaat aaatctctca
     2221 gatcatgtct catgcattta atttaatttt tttatttatt catttttatt tatttatttt
     2281 attatttatt ttattttaat atttatttta ttttatttta ttttatttta cagtctgaca
     2341 tactggacca ataaatctct cagatcatgt ctcatgcatt ttacttttat tttattagaa
     2401 ttagaaagat caaaggaaca acttttaaaa tattaattct gtatcaaaat ctcttttgat
     2461 acatttaatt gatttaaaaa agcagttcac ccaagaaaca tttcctcaca gtcgaatggt
     2521 tgtaaacttt tatgaattac tttcacagaa aaagattttt ggaagaatat tggaaaaaaa
     2581 gcagccattg acttccatag taacaacaaa aaatactatg gaagtcaatg gctgtttttt
     2641 caccattcgg tatcttcatt ctggagcaga attttttggg tgatctgtcc ctttaagtcg
     2701 tcaaatcctg gcgcaatatt ccacatgcac tgacgagtct ttatttttgg tctgctactg
     2761 ctgtgtgtgt gagggcattt acctcatctt ctgctggagt tgatgaacgg tgggttgttc
     2821 aaacagcagc aggtcattga ctgaactcct ctcgatataa aagctgcaga tctgaagctg
     2881 accttcactt tgtgttgagc ggatctaatt cgatctagag gatcagcttg ggctgcaggt
     2941 cgagggacct aataacttcg tatagcatac attatacgaa gttatattaa gggttccgga
     3001 tcctcgagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     3061 agctggacgg cgacgtaaac ggccacaagt tcagcgtgag gggcgagggc gagggcgatg
     3121 ccaccaacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     3181 ggcccaccct cgtgaccacc ctgagccacg gcgtgcagtg cttcgcccgc taccccgacc
     3241 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     3301 ccatcttctt caaggacgac ggcacctaca agacccgcgc cgaggtgaag ttcgagggcg
     3361 acaccctggt gaaccgcatc gagctgaagg gcgtcgactt caaggaggac ggcaacatcc
     3421 tggggcacaa gctggagtac aacttcaaca gccacaacat ctatatcatg gccgtcaagc
     3481 agaagaacgg catcaaggtg aacttcaaga tccgccacaa cgtggaggac ggcagcgtgc
     3541 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     3601 acagccacta cctgagcacc cagtccgtgc tgagcaaaga ccccaacgag aagcgcgatc
     3661 acatggtcct gctggagttc cgcaccgccg ccgggatcac tctcggcatg gacgagctgt
     3721 acaagtaact cgagggatct ttgtgaagga accttacttc tgtggtgtga cataattgga
     3781 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
     3841 tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga
     3901 atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc
     3961 atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag
     4021 aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt
     4081 gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact
     4141 gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta
     4201 taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa
     4261 ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata
     4321 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
     4381 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     4441 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4501 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     4561 tcaatgtatc ttatcatgtc tggatctgac atggtaagta agcttatccc cggagcttgg
     4621 gctgcaggtc gagggaccta ataacttcgt atagcataca ttatacgaag ttatattaag
     4681 ggttccggat cgatcccctc gacggatcca ccggtggccg gccatggcaa ctcaagcaga
     4741 tctaatggag ctggacatgg ccatggagcc agaccgaaaa gcagcagtca gtcactggca
     4801 gcaacagtct tacctggatg ctgggattca tgctggagca accgccacag caccagcttt
     4861 gagtggcaaa ggaaacccag aggatgaaga tgtggatacc aaccaagttt tgtatgagtg
     4921 ggagcagggc ttctctcagt ccttcactca agatcaagtg gctgatattg atggccagta
     4981 cgccatgaca agagcccagc gagttcgtgc tgcaatgttt ccagaaaccc ttgatgaagg
     5041 catgcagatt ccatccacac aatttgactc tgcacaccca acaaatgtgc aacgtttagc
     5101 agagccttcc cagatgctca aacacgctgt ggtcaacttg atcaattacc aggatgatgc
     5161 tgaattggcc actcgagcaa tccccgagct gacgaaactg cttaatgacg aggaccaggt
     5221 tgtagttaac aaggctgctg taatggttca ccagctgtca aagaaggaag cctcacgcca
     5281 tgctataatg cgctcaccac agatggtgtc tgctatagtt cgtacaatgc agaacacaaa
     5341 tgatgtagag actgcacgat gcactgcggg aacacttcac aacctttctc accatcgtga
     5401 aggcttgttg gccatattta aatctggagg cattcctgct ctagttaaaa tgcttggctc
     5461 accagttgac tctgtactat tctatgccat cacaacactg cacaatctac ttctccatca
     5521 agaaggagca aaaatggctg tacgtctggc tggtggcttg cagaaaatgg ttgcccttct
     5581 caataaaact aatgtcaagt ttttggcaat tacaacagac tgtcttcaaa tattggccta
     5641 tggaaatcaa gaaagcaagt tgataattct tgcaagtggg ggaccacaag cgttggtaaa
     5701 tatcatgagg acttacagtt atgagaaact tctgtggacc acaagtcggg tgcttaaggt
     5761 gctatccgtg tgctctagca acaagcctgc tatagttgaa gctggtggaa tgcaagcttt
     5821 aggactccat ctcacagact caagccaacg tttggttcag aattgtcttt ggacactaag
     5881 aaacctttca gatgcagcaa ctaaacagga gggaatggaa ggtcttcttg gaaccctggt
     5941 tcagcttctt ggatcagatg acattaatgt tgtaacttgt gcagctggta tattgtccaa
     6001 cctcacctgc aacaactaca aaaacaaaat gatggtttgt caagttggag gcattgaagc
     6061 acttgttcgc acagttctac gtgctgggga tagagaagac attactgaac ctgcaatttg
     6121 tgctctgcgt caccttacca gcaggcacca ggaagcagag atggctcaaa atgcagtacg
     6181 tctccattat ggacttccag tggtgatgaa actcttgcac ccgccttcac actggccttt
     6241 gataaaggct actgttggct tgatccgcaa tcttgccctt tgtcccgcca atcatgctcc
     6301 actgcgtgaa cagggtgcca ttccccgact tgttcagttg cttgttcgtg cacaccagga
     6361 cacgcagagg cgcacttcaa tgggtggaac acaacagcag tttgtggagg gtgtccgcat
     6421 ggaggagatt gttgaaggct gcacaggtgc tcttcacatt ttggctcgtg acatccacaa
     6481 ccgaattgta atcagaggtc ttaacaccat tccattattt gtgcagttgc tgtactctcc
     6541 tattgaaaat atccaaagag tggcagcagg tgtgctgtgt gagctggcgc aagacaaaga
     6601 ggctgctgaa gctattgaag ctgaaggtgc aactgctcct cttactgagc tgcttcactc
     6661 tagaaatgaa ggtgtcgcaa cttatgcagc tgctgttctg ttccgtatgt ctgaggacaa
     6721 accccaggac tacaagaagc gtctgtcagt tgagttgacc agctctctct tcaggaccga
     6781 gccaatgcca tggaatgagg ctgcagacct tggtcttgat attggtgccc aaggcgaacc
     6841 tcttggctac agacaggatg attcaagcta ccgatctttc catgctgctg gctatggtca
     6901 ggatgcaatg ggtatggact ccatgatgga tcatgacatg ggaggtcacc acccaggggc
     6961 agactatcca gttgatggac ttcctgattt gagtcatgcc caagatctta tggatggact
     7021 acctccaggt gatagcaacc aactggcctg gtttgacact gacttgtaag cggccgcgac
     7081 tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     7141 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     7201 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7261 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taatcgaggg
     7321 ggggcccggt acccaccgcg gtggcggcga tctctcccca gcatgcctgc tattgtcttc
     7381 ccaatcctcc cccttgctgt cctgccccaa cccacccccc agaatagaat gacacctact
     7441 cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc
     7501 cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac
     7561 agtcgaggct gatcagcggg ccgctttact tgtacagctc gtccatgccg ccggtggagt
     7621 ggcggccctc ggcgcgttcg tactgttcca cgatggtgta gtcctcgttg tgggaggtga
     7681 tgtccaactt gatgttgacg ttgtaggcgc cgggcagctg cacgggcttc ttggccttgt
     7741 aggtggtctt gacctcagcg tcgtagtggc cgccgtcctt cagcttcagc ctctgcttga
     7801 tctcgccctt cagggcgccg tcctcggggt acatccgctc ggaggaggcc tcccagccca
     7861 tggtcttctt ctgcattacg gggccgtcgg aggggaagtt ggtgccgcgc agcttcacct
     7921 tgtagatgaa ctcgccgtcc tgcagggagg agtcctgggt cacggtcacc acgccgccgt
     7981 cctcgaagtt catcacgcgc tcccacttga agccctcggg gaaggacagc ttcaagtagt
     8041 cggggatgtc ggcggggtgc ttcacgtagg ccttggagcc gtacatgaac tgaggggaca
     8101 ggatgtccca ggcgaagggc agggggccac ccttggtcac cttcagcttg gcggtctggg
     8161 tgccctcgta ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg
     8221 agccctccat gtgcaccttg aagcgcatga actccttgat gatggccatg ttatcctcct
     8281 cgcccttgct caccatgttg tcagacctgg taactcctta ctgtaactta ttgcacctga
     8341 tgaacaaaag atgtgaactg aacgcaagta ggaggctaat agaggctagt acagtacaaa
     8401 gatccccaaa tgatgtgtgc cggccttagt ggaggcagac ccctggttat atacctgtgc
     8461 tgcagaaaga ggggcttaca aatggatctc tctggaatgg cattatctca tggtgggaac
     8521 agtgttgtca gcagaatcag cggggtggac tctgacctga gaatgacagt ctagcactga
     8581 atggctcaga caatactctt gtggccaact atcaccggaa aataagtgcc tgccaaaaca
     8641 aaccagacca gatgaagcta catggctgta gaacgtcagt atttaatgtg ttgtggccac
     8701 ttggttcacc agatttaaga gctaattttt ttaaatatca cagataatca atcgcttttt
     8761 ttttttcttg actgaaaagc ttagactgga aaattctttg aaaaagtttg cactacagaa
     8821 aataaataca taacatcctg cactgaatgc acactattaa tggtacccag cttttgttcc
     8881 ctttagtgag ggttaattgc gcgctaggga taacagggta atacgcgctc actggccgtc
     8941 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     9001 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     9061 cagttgcgca gcctgaatgg cgaatggaaa ttgtaagcgt taatattttg ttaaaattcg
     9121 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     9181 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     9241 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     9301 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     9361 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     9421 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     9481 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     9541 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     9601 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     9661 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     9721 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     9781 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     9841 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     9901 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     9961 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
    10021 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
    10081 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
    10141 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
    10201 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
    10261 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    10321 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    10381 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    10441 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    10501 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    10561 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    10621 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    10681 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    10741 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    10801 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    10861 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    10921 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    10981 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    11041 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    11101 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    11161 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    11221 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    11281 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    11341 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    11401 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    11461 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    11521 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    11581 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    11641 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
    11701 attacgccaa gcgcgt
//
