LOCUS       sequence-393628-       10560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter 
            driving tamoxifen-inducible Cre recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10560)
  AUTHORS   Kalasekar SM, Kotiyal S, Conley C, Phan C, Young A, Evason KJ
  TITLE     Heterogeneous beta-catenin activation is sufficient to cause 
            hepatocellular carcinoma in zebrafish.
  JOURNAL   Biol Open. 2019 Oct 17;8(10):bio047829. doi: 10.1242/bio.047829.
  PUBMED    31575545
REFERENCE   2  (bases 1 to 10560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol 
            Open."; date: "2019-10-17"; volume: "8(10):bio047829. doi"; pages: "
            10.1242/bio.047829"
COMMENT     Sequence Label: pKE13-ISceI-fabp10a:CreERT2
FEATURES             Location/Qualifiers
     source          1..10560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     43..62
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        43..61
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2960..2980
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2981..3688
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3126..3144)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3394..3413
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3698..4726
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             4739..5674
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             4787..5680
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     polyA_signal    5978..6112
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6015..6034)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6069..6088
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    6130..6177
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     polyA_signal    6201..6408
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     6397..6414
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(6432..7145,7146..7148,7149..7151))
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6477..6498)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6826..6845
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(7741..7761)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7741..7759)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7792..7809)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7792..7808)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7801..7823)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(7949..8404)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8086..8107)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8298..8317
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8431..8535
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8536..8604,8605..9396)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8754..8773)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9567..10155
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10056..10075
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10309..10326
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10443..10464
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10479..10484,10485..10502,10503..10509)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10517..10533
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10522..10544
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10541..10557
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10541..10557
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gattacgcca agcgcgttag ggataacagg gtaatgcgcg cgtaatacga ctcactatag
       61 ggcgaattgg agctcggaat tcctgagcat cagaatgggg aaggaagagg atttcagtga
      121 ctttgaacga ggcatggttg ttgctgccag atgggctgct ctgagtattt cagaaactgc
      181 tgatcttcag ggattttcac gcacaaccat ctctagggtt tacagagaat gatctgaaaa
      241 gaggaaatat ccagtgagcg gcagttctgt gggtgcaaat gccttgttga tgccagagat
      301 cagaggagaa tggccagact ggttccagct gatagaaagg caacagtaac tcaaataagc
      361 actcgttaca accgagctct gcagaagagc atctctgaac acacaacacg tccaaccttg
      421 aggcagatgg gctacagcag cagaagacca caccgggtgc cgctcctgtc agctaagaac
      481 aggaaactga ggctacaatt cacacagact caccaaaact ggacaatata ttattagccc
      541 cccttagaat ttttcatttg ataatatttt tcttctggcg aaagcctcat ttgttttata
      601 tattatagaa taaaattagt ttttaatagt ttttatgcca ttttaaggtc aatattatta
      661 gcccctttaa gctatttttt ttcgatagtc tacagaacaa accatcggta tacaatgact
      721 tgcctaatta ccctaacctg cctagttacc ctaattaacc tagttaagcc tttaaatgtc
      781 actttaagct gtatagaagt gtcttgaaga atatctagtc taatattatt gactgtcatc
      841 atggcaaaga taaaataaat cagttattaa aactattatg attagaaatg tgctgaaaca
      901 atctgctctc cgataaacag aaattgaaca aaataaacag gggggctaat aaatttaagg
      961 ggttaaataa ttctgattgc aaaaaaaatg atgtctgcaa aactgttgca aataatttat
     1021 ttgtgttgat tttaagcaaa caaattaaat ttaataaaat acaacttaat ctgtttgttt
     1081 aaattcagcc ctaataaatt gtttacagcc acttaacgta aaaaaattga gtaaatccaa
     1141 ggaatcatct ctgaataatt tttttcagtg tatatatata tatatatatt cttacaaaac
     1201 aactcattta ctttagttta attttcaggg gcaaaaaact aaagtaatcg acgttgcttg
     1261 aataaaaagt gtaattaagg gaatgaggta acatttaacc atgtgtcaat gcagtttaaa
     1321 tatgccagtt agtggtatat gtttaaatgg taagctattc aaaactttaa actaacttaa
     1381 ccagcctttt gttgtcagac tgaacagact ttccatctgc attattagag actaatcttt
     1441 ggctggatga atgattcatc tgctgatatt tcagaataga cagattgagg ctgtttctaa
     1501 tatgattatg caacctgagg gtgattattt gaagcaaact ccacagacca gcaggtcatt
     1561 gaccgtcgtg tgttcaaaca gagcagaaac atttgcaaaa ctggtctgac aggagaatcc
     1621 agtccagcac aacacatatg ctgagcaaac tgaatcaatc ctgcaggtca actctcgtgc
     1681 tttaagttta ttaagattat tttatttatt tattatttta tttatctatt tatttattta
     1741 gttgtttatt tagcaattat ttgtttattt atttgtttat ttattcctgc agatcatgcc
     1801 ttgtgccttt ttacatttaa tttaattttt aatttaattt ccttttattt ttttttattt
     1861 ttttatttta ttttatttta cagtctgaca aatactgaac taaaaaccct cagatcatgt
     1921 cttatgcatt tcattttatt ttatttcatt ttatattatt aattttaata tttttatttt
     1981 acagtctgac aaatactgaa ttaaaaacca tcagatcatg tctcatgcat ttaacttaac
     2041 tttatttaat tcaattaaat tgtttgtttg tttgtttcct tgcatttgtt tgtttgtttt
     2101 ttacaatctg acatactgga ccgaaaaaac tcagatcatg tcttatgcat tttattttat
     2161 tttatttttt attttattgt ctattttatt ttatttcatc ttattttatt ttacagtctg
     2221 acatatggac caataaatct ctcagatcat gtctcatgca tttaatttaa tttttttatt
     2281 tattcatttt tatttattta ttttattatt tattttattt taatatttat tttattttat
     2341 tttattttat tttacagtct gacatactgg accaataaat ctctcagatc atgtctcatg
     2401 cattttactt ttattttatt agaattagaa agatcaaagg aacaactttt aaaatattaa
     2461 ttctgtatca aaatctcttt tgatacattt aattgattta aaaaagcagt tcacccaaga
     2521 aacatttcct cacagtcgaa tggttgtaaa cttttatgaa ttactttcac agaaaaagat
     2581 ttttggaaga atattggaaa aaaagcagcc attgacttcc atagtaacaa caaaaaatac
     2641 tatggaagtc aatggctgtt ttttcaccat tcggtatctt cattctggag cagaattttt
     2701 tgggtgatct gtccctttaa gtcgtcaaat cctggcgcaa tattccacat gcactgacga
     2761 gtctttattt ttggtctgct actgctgtgt gtgtgagggc atttacctca tcttctgctg
     2821 gagttgatga acggtgggtt gttcaaacag cagcaggtca ttgactgaac tcctctcgat
     2881 ataaaagctg cagatctgaa gctgaccttc actttgtgtt gagcggatct aattcgatat
     2941 caagcttatc gatatggctc caaagaagaa gcgtaaggta atggtgagca agggcgagga
     3001 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
     3061 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
     3121 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
     3181 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
     3241 caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
     3301 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
     3361 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
     3421 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     3481 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
     3541 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
     3601 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
     3661 caccggcggc atggacgagc tgtacaaggc agctagcatg tccaatttac tgaccgtaca
     3721 ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca agaacctgat
     3781 ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc ttctgtccgt
     3841 ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc ccgcagaacc
     3901 tgaagatgtt cgcgattatc ttctatatct tcaggcgcgt ggtctggcag taaaaactat
     3961 ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc cacgaccaag
     4021 tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg ttgatgccgg
     4081 tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg ttcgttcact
     4141 catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg ggattgctta
     4201 taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata tctcacgtac
     4261 tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta gcaccgcagg
     4321 tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga tttccgtctc
     4381 tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa atggtgttgc
     4441 cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt ttgaagcaac
     4501 tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg cctggtctgg
     4561 acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt caataccgga
     4621 gatcatgcaa gctggtggct ggaccaatgt aaatattgtc atgaactata tccgtaacct
     4681 ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgatctcg agccatctgc
     4741 tggagacatg agagctgcca acctttggcc aagcccgctc atgatcaaac gctctaagaa
     4801 gaacagcctg gccttgtccc tgacggccga ccagatggtc agtgccttgt tggatgctga
     4861 gccccccata ctctattccg agtatgatcc taccagaccc ttcagtgaag cttcgatgat
     4921 gggcttactg accaacctgg cagacaggga gctggttcac atgatcaact gggcgaagag
     4981 ggtgccaggc tttgtggatt tgaccctcca tgatcaggtc caccttctag aatgtgcctg
     5041 gctagagatc ctgatgattg gtctcgtctg gcgctccatg gagcacccag tgaagctact
     5101 gtttgctcct aacttgctct tggacaggaa ccagggaaaa tgtgtagagg gcatggtgga
     5161 gatcttcgac atgctgctgg ctacatcatc tcggttccgc atgatgaatc tgcagggaga
     5221 ggagtttgtg tgcctcaaat ctattatttt gcttaattct ggagtgtaca catttctgtc
     5281 cagcaccctg aagtctctgg aagagaagga ccatatccac cgagtcctgg acaagatcac
     5341 agacactttg atccacctga tggccaaggc aggcctgacc ctgcagcagc agcaccagcg
     5401 gctggcccag ctcctcctca tcctctccca catcaggcac atgagtaaca aaggcatgga
     5461 gcatctgtac agcatgaagt gcaagaacgt ggtgcccctc tatgacctgc tgctggaggc
     5521 ggcggacgcc caccgcctac atgcgcccac tagccgtgga ggggcatccg tggaggagac
     5581 ggaccaaagc cacttggcca ctgcgggctc tacttcatcg cattccttgc aaaagtatta
     5641 catcacgggg gaggcagagg gtttccctgc cacagcttga gcggccgccg aaatgaccga
     5701 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     5761 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     5821 catgctggag ttcttcgccc accccccgga tctaagctct agataagtaa tgatcataat
     5881 cagccatatc acatctgtag aggttttact tgctttaaaa aacctcccac acctccccct
     5941 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     6001 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6061 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgggggta
     6121 ccgcgtcgag aagttcctat tccgaagttc ctattctcta gaaagtatag gaacttcggg
     6181 cccgcggtgg cggcgatctc tccccagcat gcctgctatt gtcttcccaa tcctccccct
     6241 tgctgtcctg ccccacccca ccccccagaa tagaatgaca cctactcaga caatgcgatg
     6301 caatttcctc attttattag gaaaggacag tgggagtggc accttccagg gtcaaggaag
     6361 gcacggggga ggggcaaaca acagatggct ggcaactaga aggcacagtc gaggctgatc
     6421 agcgggccgc tttacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg
     6481 aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg
     6541 tagctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc
     6601 tggtagtggt cggcgagctg cacgccgccg tcctcgatgt tgtggcggat cttgaagttg
     6661 gccttgatgc cgttcttctg cttgtcggcg gtgatataga cgttgtggct gttgtagttg
     6721 tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg
     6781 atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg
     6841 tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag
     6901 aagtcgtgct gcttcatgtg gtcggggtag cgggcgaagc actgcaggcc gtagcccagg
     6961 gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat cagcttcagg
     7021 gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg
     7081 tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc
     7141 ttgctcacca tgttgtcaga cctggtaact ccttactgta acttattgca cctgatgaac
     7201 aaaagatgtg aactgaacgc aagtaggagg ctaatagagg ctagtacagt acaaagatcc
     7261 ccaaatgatg tgtgccggcc ttagtggagg cagacccctg gttatatacc tgtgctgcag
     7321 aaagaggggc ttacaaatgg atctctctgg aatggcatta tctcatggtg ggaacagtgt
     7381 tgtcagcaga atcagcgggg tggactctga cctgagaatg acagtctagc actgaatggc
     7441 tcagacaata ctcttgtggc caactatcac cggaaaataa gtgcctgcca aaacaaacca
     7501 gaccagatga agctacatgg ctgtagaacg tcagtattta atgtgttgtg gccacttggt
     7561 tcaccagatt taagagctaa tttttttaaa tatcacagat aatcaatcgc tttttttttt
     7621 tcttgactga aaagcttaga ctggaaaatt ctttgaaaaa gtttgcacta cagaaaataa
     7681 atacataaca tcctgcactg aatgcacact attaatgggc ccggtaccca gcttttgttc
     7741 cctttagtga gggttaattg cgcgctaggg ataacagggt aatacgcgct cactggccgt
     7801 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     7861 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     7921 acagttgcgc agcctgaatg gcgaatggaa attgtaagcg ttaatatttt gttaaaattc
     7981 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     8041 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     8101 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     8161 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     8221 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     8281 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     8341 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     8401 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     8461 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     8521 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     8581 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     8641 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     8701 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     8761 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     8821 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     8881 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     8941 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     9001 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     9061 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     9121 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     9181 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     9241 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     9301 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     9361 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     9421 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     9481 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     9541 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     9601 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     9661 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     9721 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     9781 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     9841 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     9901 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     9961 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    10021 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    10081 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    10141 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
    10201 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
    10261 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
    10321 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
    10381 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
    10441 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
    10501 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
//
