LOCUS       sequence-393640-        9194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expressing SARS-CoV-2 XBB.1.16 spike protein for pseudovirus 
            production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9194)
  TITLE     SARS-CoV-2 variant
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pcDNA3.3_SARS2_XBB.1.16
FEATURES             Location/Qualifiers
     source          1..9194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     213..233
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(242..670)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(352..373)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     564..583
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    872..920
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     945..964
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     primer_bind     complement(4890..4914)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(4911..5114)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4940..4960)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5115..5494
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5666..5685
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5760..5864
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5865..5933,5934..6725)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6083..6102)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6896..7484
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7385..7404
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7638..7655
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7772..7793
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7808..7813,7814..7831,7832..7838)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7846..7862
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7851..7873
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7870..7886
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7870..7886
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    7935..8056
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7946..7965)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8000..8019
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(8232..9026)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8343..8362)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8953..8972
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(9093..9194,1..228))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(9107..9194,1..48)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9161..9180)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag
       61 gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg
      121 ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact
      181 tctgcctgct ggggagcctg gggactttcc acaccctaac tgacacacat tccacagaat
      241 taattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
      301 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
      361 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
      421 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
      481 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
      541 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
      601 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
      661 cagggcgcgt ggggataccc cctagagccc cagctgcgca gatctgctat ggcagggcct
      721 gccgccccga cgttggctgc gagccctggg ccttcacccg aacttggggg gtggggtggg
      781 gaaaaggaag aaacgcgggc gtattggccc caatggggtc tcggtggggt atcgacagag
      841 tgccagccct gggaccgaac cccgcgttta tgaacaaacg acccaacacc cgtgcgtttt
      901 attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt
      961 ttcagttagc ctcccccgtt tggtaccacc ggttcagtca gttagatatc aagcttccat
     1021 ggctcgagtc acttacaaca ggagccacag gaacaacagc ctttcagaca ggaacaacag
     1081 gaggtcatac aacacagcat tatggtcacc atcacaatgg caatcagtcc agcaatgaag
     1141 cccagccaga tgtaccatgg ccacttgatg tattgttcat atttgcccag ttcttggagg
     1201 tcaatcaggg actcattcag gttcttagcc acctcattca gtctgtcaat ctccttctgg
     1261 atgttcacca cagaggcatt gatgccagag atgtctccca ggtccacatc agggctggtg
     1321 tggttcttga agtatttgtc cagttcctcc ttgaaggagt ccagttcagg ttggagtggg
     1381 tcatagactg tgttgttcac aatgccaatc accacatcac agttgccaga cacaaaggtg
     1441 ttgtctgtgg tgataatctg tggttcatag aagttcctct gggtcacaaa ccagtgggtg
     1501 ccattgctca caaacactcc ctcccttgga aagtgagcct tgccatcatg gcagatggca
     1561 ggggctgtgg tgaagttctt ctcctgggca ggcacatagg tcacatgcag gaacaccact
     1621 ccatgagggg cagactgtgg aaaactcatc aggtggtagc ccttgccaca gaagtccacc
     1681 ctcttgcttt gtcccagcac acactcactc atcttggtgg cagccaggtt ggcagatgcc
     1741 ctaatctcag cagccctgat aagttgttgg gtcacatagg tttggaggga ttggagtctg
     1801 cctgtaatca gtctgtcaat ctggacctca gcctccacct tgtccagtct gctcaggatg
     1861 tcattcagca cagaggagat ggctccaaac ttgctggaaa gttgcttcac cagggtgttc
     1921 agagcctggg cattatggtt caccacatct tggagtttgc ccagggcaga ggctgtgctg
     1981 gacagggagt cctgaatctt gccaatggca gagttgaact ggttggcaat cagtttctgg
     2041 ttctcataga gcacattctg ggtcactcca atgccattga acctgtaagc catctgcata
     2101 gcaaatggga tttggagggc tgctcctgct ccaaaggtcc agccagaggt gatggtgcca
     2161 gccagcaggg cagaggtgta ttgggcaatc atctcatctg tcagcagtgg aggcagcacg
     2221 gtcagtccat tgaacttctg ggcacaaatc aggtcccttg cagcaatgtc tcccagacag
     2281 tctccatatt gcttgatgaa gccagcatca gccagggtca ccttgttgaa cagcaggtcc
     2341 tcaatgaagg acctcttgct tggcttgctt gggtcaggca gaatctggct gaagttgaag
     2401 cctccaaagt acttgattgg aggtgtcttg taaatctgct tcacctgggc aaacacctcc
     2461 tgggtgttct tgtcctgttc cacagcaatg cctgtcagag ccctcttaag ttgggtacag
     2521 aaggagccat attggagcag caggttgcta cactctgtgc tgtctccaca gatatacatt
     2581 gtacagtcca cagaggtctt ggtcatactc acaggcagaa tttctgtggt cacagagatg
     2641 gtgaagttgg ttgggatggc aatgctgttg ttgctgtaag ccacagagtt ctctgctccc
     2701 agactcattg tgtaggcaat gatgctctgg cttgccacag accttgccct cctatggctc
     2761 ttggtctggg tctggtagga ggcacagatg cctgctccaa ttgggatgtc acactcatag
     2821 gagttgttca catactctgc tccaatcaga cagccagccc tggtctggaa cacattgctg
     2881 cctgtgctgt agaccctcca ggttggtgta agttggtcag catggatagc cacaggcacc
     2941 tcagtacagt tcacaccctg gtagagcaca gccacctggt tgctggtgtt ggtgccaggt
     3001 gtaatcacag acactcctcc aaaggaacat ggtgtgatgt ccagaatctc caaggtctgt
     3061 gggtccctca cagcatctgt ggtgtcagca atgtccctgc caaactgttg gaatggcagg
     3121 aacttcttgt tgctctctgt cagcactcct gtgcctgtca gtccattgaa gttgaagttc
     3181 acacacttgt tcttcaccag gttggtgctc ttctttggtc cacacactgt ggcaggggca
     3241 tggagcagtt caaaggacag caccaccacc ctgtatggtt ggtggcccac tccataggtt
     3301 ggtctgaagc cataggactg gagtggagag taacagttcg ggcccgccac tccattacat
     3361 ggtctgttgc cagcctggta aatctctgtg ctgatgtccc tctcaaatgg tttcagcttg
     3421 ctcttcctga acagtctgta gaggtagttg tagttgcctg acggcttgct gtccagcttg
     3481 ttgctgttcc aggcaatcac acagcctgtg aagtcatcag gcagtttgta gttgtagtca
     3541 gcaatgttgc ctgtttgtcc aggggcaatc tgactcacct catttcccct aatcacaaag
     3601 gagtcagcat agacattggt gaaacacagg tcattcagtt tggttgggct cactccataa
     3661 cacttgaagg cgaagaaggg ggcaaagttg tagatcacag agtagtcagc cacacagttg
     3721 ctaatcctct tcctgttcca ggcatagaca gaggcaaacg tggtggcatt gaacacctcg
     3781 tgaaatggac acaggttggt gatgtttgga aacctcacaa tgctctctgt tggttggacc
     3841 ctgaagttgc tggtctggta gatgcccttc tccactgtga aggatttcag ggtacacttg
     3901 gtctcagaca gtgggtccag ggcacagtcc acagcatctg tgatggtgcc attctcattg
     3961 tatttcagca ggaaggtcct tggttggagg tagcccacat agtaggctgc tgctcctgct
     4021 gtccagccag aggaggagtc tacaggtgtc aggtaggacc tgtgcagagc cagcagggtc
     4081 tggaacctgg tgatgttgat gccaattggc aggtccacca gtggttccaa ggcagagaag
     4141 ccctgtggca ggtccctctc caggttgatt ggtgtgtgtt tgctgtaaat cttgaagtag
     4201 ccatcaatgt tcttgaacac aaactccctc aggttcttga agttgccctc cttgcccacc
     4261 aagtccatca ggaatggttg gctcacatat tcaaaggtac agttgttggc agaggagtag
     4321 accctgaact cagactccat ccaggacttg ttgttcttct ggtagacgtc caggaatggg
     4381 tcattacaga actggaactc acacacctta atcaccacat tggtggcatt gttcacaatc
     4441 agcagggact gggtcttgct gtccagggtg gtgccaaaaa tccagcccct gatgatgttg
     4501 ctcttctctg tgctggcaaa gtagactcca tcattgaatg gcagcgcagg gttgtcaaac
     4561 ctcttggtgc cattggtgcc agacacatgg atggcatgga accaggtcac attgctgaag
     4621 aatggcagga acaggtcctg ggtgctgtgc agcacagagg acctgaacac cttgtcaggg
     4681 tagtagactc ccctggtgaa ggagttggtg taggattggg tcctggtgat caggttcaca
     4741 cactggctgg acaccagtgg cagcagcacc aggaacacaa acatggatcc gaattctcta
     4801 gagttaacct taaggagctc agtccggagg ctggatcggt cccggtgtct tctatggagg
     4861 tcaaaacagc gtggatggcg tctccaggcg atctgacggt tcactaaacg agctctgctt
     4921 atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta
     4981 cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg
     5041 gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc
     5101 caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
     5161 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
     5221 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
     5281 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
     5341 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
     5401 accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt
     5461 gattactatt aataactagt caataatcaa tgtcaacgcg tatatctggc ccgtacatcg
     5521 cgaagcagcg caaaacgcct aaccctaagc agattcttca tgcaattgtc ggtcaagcct
     5581 tgccttgttg tagcttaaat tttgctcgcg cactactcag cgacctccaa cacacaagca
     5641 gggagcagat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     5701 aggggatcgg gagatctccc gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgc
     5761 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5821 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5881 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5941 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     6001 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     6061 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     6121 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     6181 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     6241 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     6301 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     6361 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     6421 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     6481 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     6541 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     6601 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6661 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     6721 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     6781 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6841 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6901 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6961 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7021 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     7081 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     7141 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     7201 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     7261 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     7321 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     7381 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     7441 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     7501 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     7561 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     7621 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     7681 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     7741 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     7801 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     7861 aatttcacac aggaaacagc tatgaccatg attacgccaa gctctagcta gaggtcgacg
     7921 gtatacagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     7981 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     8041 ctgcaataaa caagttgggg tgggcgaaga actccagcat gagatccccg cgctggagga
     8101 tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg
     8161 tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgcgaaccc
     8221 cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg
     8281 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca
     8341 gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca
     8401 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg
     8461 ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt
     8521 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct
     8581 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta
     8641 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca
     8701 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc
     8761 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc
     8821 cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg caccggacag gtcggtcttg
     8881 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg
     8941 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct
     9001 gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc ttgatcagat
     9061 ccgaaaatgg atatacaagc tcccgggagc tttttgcaaa agcctaggcc tccaaaaaag
     9121 cctcctcact acttctggaa tagctcagag gcagaggcgg cctcggcctc tgcataaata
     9181 aaaaaaatta gtca
//
