LOCUS       sequence-393643-        3396 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express full length mouse PLD3 protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Yuan M, Peng L, Huang D, Gavin A, Luan F, Tran J, Feng Z, Zhu X, 
            Matteson J, Wilson IA, Nemazee D
  TITLE     Structural and mechanistic insights into disease-associated 
            endolysosomal exonucleases PLD3 and PLD4.
  JOURNAL   Structure. 2024 Jun 6;32(6):766-779.e7. doi: 
            10.1016/j.str.2024.02.019. Epub 2024 Mar 26.
  PUBMED    38537643
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.str.2024.02.019"; journalName: "Structure"; date: 
            "2024-06-6- 6"; volume: "32"; issue: "6"; pages: "766-779.e7"
COMMENT     Sequence Label: SuExp-mPLD3
FEATURES             Location/Qualifiers
     source          1..3396
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        243..446
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     397..417
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     443..467
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    1992..2126
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2016..2035)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2070..2089
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(2169..2757)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2249..2268)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2782..3156)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(3157..3220)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        join(3259..3396,1..242)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
       61 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
      121 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
      181 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
      241 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
      301 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
      361 actttccaaa atgtcgtaat aaccccgccc cgttgacgca aatgggcggt aggcgtgtac
      421 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgtc
      481 gagccgagtg gttgtgcctc catagaagcc accatgaagc ccaaactgat gtaccaggag
      541 ctgaaggtgc ctgttgagga gcctgcggga gaactgccct tgaatgaaat cgaggcatgg
      601 aaggcagcag agaagaaagc ccgctgggtc ctcctggtcc tgatcctggc ggtagtgggc
      661 ttcggtgccc tgatgactca gctgtttcta tgggaatacg gggacttaca tctatttgga
      721 ccaaatcagc gcccagcccc ctgctatgac ccctgcgagg cggtgctggt ggagagcatt
      781 cctgagggtc tggagtttcc caatgccacc acgagcaacc cctccactag ccaggcctgg
      841 ttgggcctcc ttgccggtgc tcacagcagc ctggacatcg catccttcta ctggaccctc
      901 acaaataatg acacccacac gcaagagccc tctgcccagc agggtgaaga ggttcttcag
      961 cagcttcagg cactggcacc tcgaggtgta aaggttcgca tcgctgtgag caaacccaat
     1021 gggcctctgg ctgatctgca gtctctgcta cagagtggtg cccaggtccg catggtggac
     1081 atgcagaagc tgactcatgg tgtcctgcac accaagttct gggtggtgga tcagacccac
     1141 ttctacctgg gcagtgccaa catggactgg cggtcgctga cccaggtcaa ggagctgggc
     1201 gtggtcatgt acaactgcag ctgcctcgcc cgggacctca ccaagatctt tgaagcctat
     1261 tggttcctgg gccaggcagg tagctccatc ccatcaacct ggccgcggtc ctttgacacc
     1321 cgatataatc aagaaacacc aatggagatc tgcctcaatg gcaccccagc tctggcctac
     1381 ctagcgagtg cacccccacc actgtgtcca agtggccgca ccccagacct gaaggctctg
     1441 ctcaacgtgg tggacagtgc ccgaagcttc atctacattg cggttatgaa ctacctgccc
     1501 accatggagt tctctcatcc acgcaggttc tggccagcga tcgatgatgg gctgagacgg
     1561 gctgcgtatg aacgaggcgt caaagtgcgc ctgctcatca gctgctgggg acactctgat
     1621 ccatcgatgc ggtccttcct gctctccctg gctgcacttc atgacaacca tactcactcc
     1681 gacatccagg tgaaactgtt tgtggtccct acggatgagt cccaggcccg aatcccctat
     1741 gcccgtgtca accacaacaa gtacatggtg actgaacgtg cctcatacat tggaacctcc
     1801 aactggtctg gaagctactt cacggagaca gcaggcacct ccctgctggt gacacagaac
     1861 gggcatggtg gcttgcgcag tcagctggag gctgttttcc tgagagactg ggaatcccca
     1921 tacagccacg atctcgacac ctcagccaac agtgtgggca atgcctgccg cctgctttga
     1981 atctagaagc tgatccagac atgataagat acattgatga gtttggacaa accacaacta
     2041 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     2101 ccattataag ctgcaataaa caagttggat atcctcgaga ctagtgggcc cgtttaaaca
     2161 catgtgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     2221 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     2281 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     2341 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     2401 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     2461 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     2521 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     2581 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     2641 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     2701 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     2761 gatcctttga tcttttctac gtcagtcctg ctcctcggcc acgaagtgca cgcagttgcc
     2821 ggccgggtcg cgcagggcga actcccgccc ccacggctgc tcgccgatct cggtcatggc
     2881 cggcccggag gcgtcccgga agttcgtgga cacgacctcc gaccactcgg cgtacagctc
     2941 gtccaggccg cgcacccaca cccaggccag ggtgttgtcc ggcaccacct ggtcctggac
     3001 cgcgctgatg aacagggtca cgtcgtcccg gaccacaccg gcgaagtcgt cctccacgaa
     3061 gtcccgggag aacccgagcc ggtcggtcca gaactcgacc gctccggcga cgtcgcgcgc
     3121 ggtgagcacc ggaacggcac tggtcaactt ggccatactc ttcctttttc aatattattg
     3181 aagcatttat cagggttatt gtctcatgag cggatacata acgcgtcgcg aggccatatg
     3241 ggttaaccca tggccgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     3301 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     3361 cgcctggctg accgcccaac gacccccgcc cattga
//
