LOCUS       sequence-393669-       12733 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12733)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2088
FEATURES             Location/Qualifiers
     source          1..12733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5671..6259
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5724..5744)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6142..6153)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6331..6564
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6642..6763
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6679..6698)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6733..6752
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6803..6938
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6958..6977)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6959..6977)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6987..7004)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6987..7003)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6996..7018)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7145..7600
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7232..7251)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7442..7463
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7626..7730
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7731..7799,7800..8591)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7949..7968)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8762..9350
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9251..9270
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9504..9521
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9638..9659
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9674..9679,9680..9697,9698..9704)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9712..9728
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9717..9739
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9736..9752
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9736..9752
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9771..9791
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9773..9791
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9819..10045
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10046..10226
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10273..10398
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10891..11124
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11309..11353
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        11584..11794
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        11606..11802
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      11653..11788
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             11834..12436
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(11834..11853)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     12330..12350
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    12479..12596
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggg
     1201 cggtgcttgc agctccttta ccacctcttc cagccctacc atttattcta cctcagtcac
     1261 cgacagcaag gctatgcaag tggagagctg ctcctcagcc gtgggggtaa gtaacagagg
     1321 ggtaagtgaa aagcagttaa ccagtaacac agttcagcag catccatcaa caccgaagag
     1381 gcacacagtc ttgtacatct caccaccacc tgaggacttg ctggataaca gtcggatgtc
     1441 ctgccaggat gaggggtgtg gattggaatc tgagcagagc tgcagtatgt ggatggagga
     1501 ttccccctcc aacttcagta acatgagcac cagttcctac aatgataaca ctgaggtacc
     1561 tcgtaaatca cgaaaacgaa atccaaagca gaggccgggg gtcaaacgac gagattgtga
     1621 agaatctaat atggatatat ttgatgccga cagtgccaaa gcacctcact atgtgctttc
     1681 tcagcttacc acggacaaca aaggcaactc aaaagcggga aatggaacat tggaaaacca
     1741 aaaaggaact ggagtaaaga agagccctat gttgtgtgga caatatcctg ttaaaagtga
     1801 gggaaaggag ctgaagatag ttgtacaacc tgagacacag caccgagctc ggtacctgac
     1861 tgagggcagc cgtggctcag tgaaagatag aacacagcaa ggctttccta cagtaaagct
     1921 ggaaggccat aatgaacctg tagtgttgca agtgtttgtg ggcaacgact ctggacgagt
     1981 gaaaccacat ggattttatc aggcctgcag agtaactgga cgaaatacaa ctccttgcaa
     2041 agaagtggac attgaaggca ctactgttat agaagtcggc cttgatccta gcaacaacat
     2101 gacactggcg gtggactgcg tagggatatt gaaattgagg aatgctgatg tcgaagccag
     2161 aataggaatt gctggttcca agaagaaaag cactcgtgcc agattggttt ttcgagttaa
     2221 tatcatgagg aaagatggct ccactttgac actgcaaaca ccctcttctc caattttgtg
     2281 tactcagcca gcaggagtgc cagaaatctt aaagaaaagc ttgcatagct gttcagtgaa
     2341 aggagaagaa gaagtgtttt taatcggcaa gaactttctg aaaggaacta aagttatttt
     2401 ccaagaaaat gtttctgatg aaaactcttg gaagtcagaa gctgaaattg atatggaact
     2461 atttcatcag aatcatctta ttgtgaaggt tcctccctat catgaccaac atataacttt
     2521 gcctgtgtca gtgggaatat atgtagtgac aaatgctgga agatctcatg atgttcaacc
     2581 attcacttac actccagacc cagcagcagc tggtgctttg aatgtaaatg tgaagaagga
     2641 aatatctagt ccagcaagac cttgctcttt tgaagaggcc atgaaagcaa tgaaaactac
     2701 tggatgtaat ttagataagg taaatattat ccctaatgcc ctgatgactc cactcatacc
     2761 aagcagtatg attaagagtg aagatgttac tccaatggaa gtaacagcag aaaaaagatc
     2821 ttccactatt tttaagacta caaagtctgt tggatcaact cagcaaacat tagaaaacat
     2881 ctcaaacata gcaggaaatg gctctttttc atcaccatca tcttcccacc taccttctga
     2941 aaatgaaaaa cagcagcaga ttcagcccaa ggcatacaac ccagagaccc tgacaactat
     3001 tcaaacccag gacatctcac agcctggtac ttttccagca gtttctgctt ctagtcagct
     3061 gcccaacagc gatgcactat tgcagcaggc tacacagttt cagacaagag aaactcagtc
     3121 tagagagata ttacagtcag atggtacagt ggttaatttg tcacaactga ctgaggcatc
     3181 acaacaacag cagcagtcac cactacaaga acaagcacag actttacagc agcagatttc
     3241 atcaaatatt tttccatcac caaatagtgt gagtcagctt cagaatacta ttcagcagct
     3301 gcaagcaggg agtttcacag gcagtactgc tagtggcagc agtggaagtg ttgacttggt
     3361 ccaacaagtt ttagaggcac agcagcagtt atcttcagtt ttattttctg ctccagatgg
     3421 taatgagaat gttcaagagc agcttagtgc agatattttt caacaagtca gtcaaattca
     3481 gagtggtgta agccctggaa tgttttcctc aacagagcca acagtccata ccagaccaga
     3541 taatttatta cctggaagag ctgaaagtgt tcatccacag tctgaaaaca cgttatctaa
     3601 tcaacagcag cagcagcagc agcaacagca agtgatggaa tcttcagccg caatggtgat
     3661 ggagatgcaa cagagtatct gccaggcagc tgcccagatt cagtcagagt tattcccttc
     3721 aactgcttca gcaaatggaa accttcagca atcgccagtt taccagcaga cttctcacat
     3781 gatgagtgca ttgtctacca atgaggatat gcaaatgcag tgtgaattgt tttcttctcc
     3841 tcctgcagtt tctggaaatg aaacttctac aactaccaca cagcaggttg caacccctgg
     3901 cactaccatg tttcagacat caagttcagg agatggagaa gaaactggaa cacaagcaaa
     3961 acagattcag aacagtgtct ttcagaccat ggtccaaatg caacatagtg gggacaatca
     4021 acctcaagtt aacctttttt catccacaaa aagtatgatg agtgttcaga atagtggtac
     4081 ccaacaacaa ggtaatggtt tattccagca agggaatgag atgatgtcac ttcaatctgg
     4141 aaattttttg cagcagtctt ctcattcaca ggcccaactt tttcatcctc aaaatcctat
     4201 tgccgatgct cagaaccttt cccaggaaac tcaaggttct ctctttcata gtccaaatcc
     4261 tattgtccac agtcagactt ctacaacctc ctctgaacaa atgcagcctc caatgtttca
     4321 ctctcaaagt accattgctg tgttacaggg ctcttcagtt cctcaagacc agcagtcaac
     4381 caacatattt ctttcccaga gtcccatgaa taatcttcag actaacacag tagcccaaga
     4441 agcatttttt gcagcaccga actcaatttc tccacttcag tcaacatcaa acagtgaaca
     4501 acaagctgct ttccaacagc aagctccaat atcacacatc cagactccta tgctttccca
     4561 agaacaggca caacccccgc agcagggttt atttcagcct caggtggccc tgggctccct
     4621 tccacctaat ccaatgcctc aaagccaaca aggaaccatg ttccagtcac agcactcaat
     4681 agttgccatg cagagtaact ctccatccca ggaacagcag cagcagcagc aacagcagca
     4741 gcaacagcag cagcaacaac aacagagcat tttattcagt aatcagaata ccatggctac
     4801 aatggcgtct ccaaagcaac caccaccaaa catgatattc aacccaaatc aaaatccaat
     4861 ggctaatcag gagcaacaga accagtcaat ttttcaccaa caaagtaaca tggccccaat
     4921 gaatcaagag caacagccca tgcaatttca gagtcagtcc acagtttcct cacttcagaa
     4981 cccaggtcct acccagtcgg aatcatcaca gacccccttg ttccatagct ctcctcagat
     5041 tcagttggta caagggtcac ctagttctca agagcagcaa gtaactctct tcttatctcc
     5101 agcatccatg tctgccttgc agaccagtat aaatcaacaa gatatgcaac agtctcctct
     5161 ttattcccct cagaacaaca tgcctggaat tcaaggagcc acatcttcgc ctcaaccaca
     5221 ggctacttta tttcacaaca cagcaggagg cacaatgaac caactgcaga attctcctgg
     5281 ctcatctcag cagacatcag gaatgttctt atttggcatt caaaataact gtagtcagct
     5341 tttaacctct ggaccagcta cattgcctga tcagttgatg gccataagtc agccaggcca
     5401 accacaaaac gagggccagc cacctgtgac aacacttctt tctcagcaaa tgccagagaa
     5461 ttctccactg gcatcctcta taaacaccaa ccagaacatc gaaaagattg atttgcttgt
     5521 ttcattgcaa aaccaaggga acaacttgac tggctccttt tagtaatgaa ctagtccgta
     5581 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg agtagacatg
     5641 ttcaatcaac gcaaaacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     5701 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     5761 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     5821 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     5881 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     5941 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     6001 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     6061 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     6121 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     6181 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     6241 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     6301 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     6361 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     6421 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     6481 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     6541 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     6601 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     6661 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6721 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     6781 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     6841 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     6901 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     6961 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     7021 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     7081 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     7141 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     7201 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     7261 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     7321 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     7381 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     7441 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     7501 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     7561 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     7621 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     7681 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     7741 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     7801 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     7861 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     7921 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     7981 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     8041 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     8101 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     8161 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     8221 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     8281 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     8341 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     8401 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     8461 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     8521 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     8581 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     8641 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     8701 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     8761 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     8821 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     8881 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     8941 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     9001 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     9061 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     9121 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     9181 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     9241 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     9301 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     9361 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     9421 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     9481 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     9541 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     9601 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     9661 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     9721 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     9781 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     9841 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     9901 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     9961 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
    10021 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
    10081 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
    10141 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
    10201 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
    10261 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
    10321 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
    10381 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
    10441 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
    10501 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
    10561 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
    10621 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
    10681 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
    10741 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
    10801 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
    10861 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
    10921 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
    10981 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
    11041 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
    11101 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
    11161 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
    11221 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
    11281 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
    11341 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
    11401 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
    11461 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
    11521 gggacccatg cattgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
    11581 ccagcaggca gaagtatgca aagcatgcgt ctcaattagt cagcaaccat agtcccgccc
    11641 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
    11701 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
    11761 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctttc tagaggtacc
    11821 accggagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
    11881 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
    11941 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
    12001 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg
    12061 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
    12121 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
    12181 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
    12241 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
    12301 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
    12361 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
    12421 gcccggtgcc agttaacgaa ttctagatct tgagacaaat ggcagtattc atccacaatt
    12481 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
    12541 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
    12601 tttattacag ggacagcaga gatccacttt ggcgccggct cgaggcctgc aggtgcaaag
    12661 atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa
    12721 aggagtggga att
//
