LOCUS       sequence-393670-       13012 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13012)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 13012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2087
FEATURES             Location/Qualifiers
     source          1..13012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    5950..6538
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6003..6023)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6421..6432)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6610..6843
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6921..7042
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6958..6977)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7012..7031
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7082..7217
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7237..7256)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7238..7256)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7266..7283)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7266..7282)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7275..7297)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7424..7879
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7511..7530)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7721..7742
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7905..8009
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8010..8078,8079..8870)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8228..8247)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9041..9629
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9530..9549
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9783..9800
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9917..9938
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9953..9958,9959..9976,9977..9983)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9991..10007
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9996..10018
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10015..10031
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10015..10031
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10050..10070
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10052..10070
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10098..10324
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10325..10505
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10552..10677
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11170..11403
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11588..11632
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        11863..12073
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        11885..12081
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      11932..12067
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             12113..12715
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(12113..12132)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     12609..12629
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    12758..12875
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgcc
     1201 ctcggacttc atctcattgc tcagcgcgga cctagacctg gaatcgccca agtccctcta
     1261 ctcgcgagat tctctgaagt tacacccatc acagaatttt catagagctg gactattgga
     1321 agaatctgtc tatgatcttc tcccaaagga gttacagtta cctccatcta gagaaacatc
     1381 tgtagcatca atgagtcaga caagcggtgg tgaggcaggc tcgcctcctc cagctgttgt
     1441 tgctgctgat gcttcttcag ctccctcctc ttcctccatg ggcggtgctt gcagctcctt
     1501 taccacctct tccagcccta ccatttattc tacctcagtc accgacagca aggctatgca
     1561 agtggagagc tgctcctcag ccgtgggggt aagtaacaga ggggtaagtg aaaagcagtt
     1621 aaccagtaac acagttcagc agcatccatc aacaccgaag aggcacacag tcttgtacat
     1681 ctcaccacca cctgaggact tgctggataa cagtcggatg tcctgccagg atgaggggtg
     1741 tggattggaa tctgagcaga gctgcagtat gtggatggag gattccccct ccaacttcag
     1801 taacatgagc accagttcct acaatgataa cactgaggta cctcgtaaat cacgaaaacg
     1861 aaatccaaag cagaggccgg gggtcaaacg acgagattgt gaagaatcta atatggatat
     1921 atttgatgcc gacagtgcca aagcacctca ctatgtgctt tctcagctta ccacggacaa
     1981 caaaggcaac tcaaaagcgg gaaatggaac attggaaaac caaaaaggaa ctggagtaaa
     2041 gaagagccct atgttgtgtg gacaatatcc tgttaaaagt gagggaaagg agctgaagat
     2101 agttgtacaa cctgagacac agcaccgagc tcggtacctg actgagggca gccgtggctc
     2161 agtgaaagat agaacacagc aaggctttcc tacagtaaag ctggaaggcc ataatgaacc
     2221 tgtagtgttg caagtgtttg tgggcaacga ctctggacga gtgaaaccac atggatttta
     2281 tcaggcctgc agagtaactg gacgaaatac aactccttgc aaagaagtgg acattgaagg
     2341 cactactgtt atagaagtcg gccttgatcc tagcaacaac atgacactgg cggtggactg
     2401 cgtagggata ttgaaattga ggaatgctga tgtcgaagcc agaataggaa ttgctggttc
     2461 caagaagaaa agcactcgtg ccagattggt ttttcgagtt aatatcatga ggaaagatgg
     2521 ctccactttg acactgcaaa caccctcttc tccaattttg tgtactcagc cagcaggagt
     2581 gccagaaatc ttaaagaaaa gcttgcatag ctgttcagtg aaaggagaag aagaagtgtt
     2641 tttaatcggc aagaactttc tgaaaggaac taaagttatt ttccaagaaa atgtttctga
     2701 tgaaaactct tggaagtcag aagctgaaat tgatatggaa ctatttcatc agaatcatct
     2761 tattgtgaag gttcctccct atcatgacca acatataact ttgcctgtgt cagtgggaat
     2821 atatgtagtg acaaatgctg gaagatctca tgatgttcaa ccattcactt acactccaga
     2881 cccagcagct ggtgctttga atgtaaatgt gaagaaggaa atatctagtc cagcaagacc
     2941 ttgctctttt gaagaggcca tgaaagcaat gaaaactact ggatgtaatt tagataaggt
     3001 aaatattatc cctaatgccc tgatgactcc actcatacca agcagtatga ttaagagtga
     3061 agatgttact ccaatggaag taacagcaga aaaaagatct tccactattt ttaagactac
     3121 aaagtctgtt ggatcaactc agcaaacatt agaaaacatc tcaaacatag caggaaatgg
     3181 ctctttttca tcaccatcat cttcccacct accttctgaa aatgaaaaac agcagcagat
     3241 tcagcccaag gcatacaacc cagagaccct gacaactatt caaacccagg acatctcaca
     3301 gcctggtact tttccagcag tttctgcttc tagtcagctg cccaacagcg atgcactatt
     3361 gcagcaggct acacagtttc agacaagaga aactcagtct agagagatat tacagtcaga
     3421 tggtacagtg gttaatttgt cacaactgac tgaggcatca caacaacagc agcagtcacc
     3481 actacaagaa caagcacaga ctttacagca gcagatttca tcaaatattt ttccatcacc
     3541 aaatagtgtg agtcagcttc agaatactat tcagcagctg caagcaggga gtttcacagg
     3601 cagtactgct agtggcagca gtggaagtgt tgacttggtc caacaagttt tagaggcaca
     3661 gcagcagtta tcttcagttt tattttctgc tccagatggt aatgagaatg ttcaagagca
     3721 gcttagtgca gatatttttc aacaagtcag tcaaattcag agtggtgtaa gccctggaat
     3781 gttttcctca acagagccaa cagtccatac cagaccagat aatttattac ctggaagagc
     3841 tgaaagtgtt catccacagt ctgaaaacac gttatctaat caacagcagc agcagcagca
     3901 gcaacagcaa gtgatggaat cttcagccgc aatggtgatg gagatgcaac agagtatctg
     3961 ccaggcagct gcccagattc agtcagagtt attcccttca actgcttcag caaatggaaa
     4021 ccttcagcaa tcgccagttt accagcagac ttctcacatg atgagtgcat tgtctaccaa
     4081 tgaggatatg caaatgcagt gtgaattgtt ttcttctcct cctgcagttt ctggaaatga
     4141 aacttctaca actaccacac agcaggttgc aacccctggc actaccatgt ttcagacatc
     4201 aagttcagga gatggagaag aaactggaac acaagcaaaa cagattcaga acagtgtctt
     4261 tcagaccatg gtccaaatgc aacatagtgg ggacaatcaa cctcaagtta accttttttc
     4321 atccacaaaa agtatgatga gtgttcagaa tagtggtacc caacaacaag gtaatggttt
     4381 attccagcaa gggaatgaga tgatgtcact tcaatctgga aattttttgc agcagtcttc
     4441 tcattcacag gcccaacttt ttcatcctca aaatcctatt gccgatgctc agaacctttc
     4501 ccaggaaact caaggttctc tctttcatag tccaaatcct attgtccaca gtcagacttc
     4561 tacaacctcc tctgaacaaa tgcagcctcc aatgtttcac tctcaaagta ccattgctgt
     4621 gttacagggc tcttcagttc ctcaagacca gcagtcaacc aacatatttc tttcccagag
     4681 tcccatgaat aatcttcaga ctaacacagt agcccaagaa gcattttttg cagcaccgaa
     4741 ctcaatttct ccacttcagt caacatcaaa cagtgaacaa caagctgctt tccaacagca
     4801 agctccaata tcacacatcc agactcctat gctttcccaa gaacaggcac aacccccgca
     4861 gcagggttta tttcagcctc aggtggccct gggctccctt ccacctaatc caatgcctca
     4921 aagccaacaa ggaaccatgt tccagtcaca gcactcaata gttgccatgc agagtaactc
     4981 tccatcccag gaacagcagc agcagcagca acagcagcag caacagcagc agcaacaaca
     5041 acagagcatt ttattcagta atcagaatac catggctaca atggcgtctc caaagcaacc
     5101 accaccaaac atgatattca acccaaatca aaatccaatg gctaatcagg agcaacagaa
     5161 ccagtcaatt tttcaccaac aaagtaacat ggccccaatg aatcaagagc aacagcccat
     5221 gcaatttcag agtcagtcca cagtttcctc acttcagaac ccaggtccta cccagtcgga
     5281 atcatcacag acccccttgt tccatagctc tcctcagatt cagttggtac aagggtcacc
     5341 tagttctcaa gagcagcaag taactctctt cttatctcca gcatccatgt ctgccttgca
     5401 gaccagtata aatcaacaag atatgcaaca gtctcctctt tattcccctc agaacaacat
     5461 gcctggaatt caaggagcca catcttcgcc tcaaccacag gctactttat ttcacaacac
     5521 agcaggaggc acaatgaacc aactgcagaa ttctcctggc tcatctcagc agacatcagg
     5581 aatgttctta tttggcattc aaaataactg tagtcagctt ttaacctctg gaccagctac
     5641 attgcctgat cagttgatgg ccataagtca gccaggccaa ccacaaaacg agggccagcc
     5701 acctgtgaca acacttcttt ctcagcaaat gccagagaat tctccactgg catcctctat
     5761 aaacaccaac cagaacatcg aaaagattga tttgcttgtt tcattgcaaa accaagggaa
     5821 caacttgact ggctcctttt agtaatgaac tagtccgtaa cttgaaagta tttcgatttc
     5881 ttggctttat atatcttgtg gaaaggacga ctctaactcc ggcggcgtat cttaacgcgt
     5941 taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     6001 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     6061 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     6121 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     6181 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     6241 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     6301 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     6361 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     6421 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     6481 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcgt
     6541 cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     6601 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     6661 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6721 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     6781 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     6841 gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     6901 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     6961 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     7021 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     7081 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7141 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     7201 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     7261 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     7321 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     7381 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     7441 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     7501 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     7561 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     7621 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     7681 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     7741 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     7801 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     7861 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     7921 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     7981 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     8041 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     8101 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     8161 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     8221 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     8281 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     8341 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     8401 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     8461 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     8521 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     8581 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     8641 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     8701 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     8761 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     8821 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     8881 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     8941 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     9001 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     9061 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     9121 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     9181 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     9241 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     9301 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     9361 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     9421 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     9481 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     9541 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     9601 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     9661 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     9721 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     9781 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     9841 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     9901 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     9961 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
    10021 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
    10081 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
    10141 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
    10201 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
    10261 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
    10321 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
    10381 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
    10441 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
    10501 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
    10561 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
    10621 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
    10681 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
    10741 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
    10801 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
    10861 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
    10921 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
    10981 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
    11041 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
    11101 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
    11161 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
    11221 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
    11281 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
    11341 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
    11401 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
    11461 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
    11521 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
    11581 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
    11641 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
    11701 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
    11761 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccatgc attgcatctc
    11821 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
    11881 agcatgcgtc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
    11941 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
    12001 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
    12061 ggaggcctag gcttttgcaa aaagctttct agaggtacca ccggagctta ccatgaccga
    12121 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
    12181 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
    12241 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
    12301 ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg accacgccgg agagcgtcga
    12361 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
    12421 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
    12481 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
    12541 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
    12601 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
    12661 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcca gttaacgaat
    12721 tctagatctt gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
    12781 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
    12841 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
    12901 atccactttg gcgccggctc gaggcctgca ggtgcaaaga tggataaagt tttaaacaga
    12961 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa tt
//
