LOCUS       sequence-393672-       10219 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmids encode parts for syntetic genetic circuit construction in 
            eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_> 
            6XAO-2XPO::TetR-NLS-19Ster_proT::GFPint_ADHter ).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10219)
  AUTHORS   Brophy JAN, Magallon KJ, Duan L, Zhong V, Ramachandran P, Kniazev K,
            Dinneny JR
  TITLE     Synthetic genetic circuits as a means of reprogramming plant roots.
  JOURNAL   Science. 2022 Aug 12;377(6607):747-751. doi: 
            10.1126/science.abo4326. Epub 2022 Aug 11.
  PUBMED    35951698
REFERENCE   2  (bases 1 to 10219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.abo4326"; journalName: "Science"; date: 
            "2022-08-12- 12"; volume: "377"; issue: "6607"; pages: "747-751"
COMMENT     Sequence Label: JAB3494
FEATURES             Location/Qualifiers
     source          1..10219
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..23
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     CDS             1546..2256
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQNKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMEELYK"
     primer_bind     complement(1691..1709)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     terminator      2275..2527
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3449..3477)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     intron          3617..3805
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     primer_bind     4261..4281
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     promoter        6130..6176
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             6852..6872
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6928..7104
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(7184..7204)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7184..7202)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7223..7239)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7223..7239)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7236..7258)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7247..7263
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7271..7277,7278..7295,7296..7301))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7316..7337
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7454..7471)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7576..7600
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(7691..8279)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7771..7790)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8366..9160)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9657..10092
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tggcaggata tattgtggtg taacgttaag ctttggttgt cgacgagtca gtaataaacg
       61 gcgtcaaagt ggttgcagcc ggcacacacg agtcgtgttt atcaactcaa agcacaaata
      121 cttttcctca acctaaaaat aaggcaatta gccaaaaaca actttgcgtg taaacaacgc
      181 tcaatacacg tgtcatttta ttattagcta ttgcttcacc gccttagctt tctcgtgacc
      241 tagtcgtcct cgtcttttct tcttcttctt ctataaaaca atacccaaag agctcttctt
      301 cttcacaatt cagatttcaa tttctcaaaa tcttaaaaac tttctctcaa ttctctctac
      361 cgtgatcaag gtaaatttct gtgttcctta ttctctcaaa atcttcgatt ttgttttcgt
      421 tcgatcccaa tttcgtatat gttctttggt ttagattctg ttaatcttag atcgtagacg
      481 attttctggg tttgatcgtt agatatcatc ttaattctcg attagggttt catagatatc
      541 atccgatttg ttcaaataat ttgagttttg tcgaataatt actcttcgat ttgtgatttc
      601 tatctagatc tggtgttagt ttctagtttg tgcgatcgaa tttgtcgatt aatctgagtt
      661 tttctgatta acagcaccat ggcaaaggat gtggaagccg ttcccggaga aggatttcag
      721 acaagagact atcaagatcc gccaccagct ccgtttattg atggagcgga gctaaagaag
      781 tggtctttct acagagcagt tatcgcagag ttcgtagcca ctctcctctt cttatacatc
      841 accgttttga cagtcatcgg ttacaagatt cagtccgata ctgatgccgg tggcgtagat
      901 tgcggcggag ttggaatcct cggtatcgct tgggcctttg gtggtatgat cttcatcctc
      961 gtctactgca ccgccggtat ctctggtggt cacattaacc cagcggtgac atttgggcta
     1021 ttcttggcac gtaaagtgtc gttacctagg gccctattgt acataatcgc tcagtgtttg
     1081 ggtgcgattt gtggagttgg ttttgtcaaa gccttccaaa gctcttacta cacccgttac
     1141 ggaggtggag ccaactctct agccgatggc tacagcacag ggaccggtct agccgcagag
     1201 atcattggta ctttcgttct tgtctacacc gtattctctg ccactgaccc caaacgtagt
     1261 gccagagact cccacgttcc ggtgttggcg ccacttccaa tcggatttgc cgtgttcatg
     1321 gtacatttgg ctaccattcc cattaccgga accggaatta acccggcaag gagtttcgga
     1381 gctgccgtaa tctacaacaa gagcaagcca tgggatgacc actggatatt ttgggttgga
     1441 ccattcattg gagctgcgat agctgcattc taccaccaat tcgttctaag agcttcaggt
     1501 tctaagtctc ttggatcatt cagaagtgct gccaacgtcg gatccatggt gagcaagggc
     1561 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     1621 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     1681 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     1741 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     1801 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     1861 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     1921 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaataagacc
     1981 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     2041 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     2101 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     2161 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     2221 cactccaccg gcggcatgga ggagctgtac aagtaaagcg gccgcccggc tgcagatcgt
     2281 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     2341 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     2401 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     2461 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     2521 ctagatcggg gcgcgccctc gagggtctct aatgggtccc cagatttgcc ttttcaattt
     2581 cagaaagaat gctaacccac agatggttag agaggcttac gcagcagctc tcatcaagac
     2641 gatctacccg agcaataatc tccaggaaat caaatacctt cccaagaagg ttaaagatgc
     2701 agtcaaaaga ttcaggacta actgcatcaa gaacacagag aaagatatat ttctcaagat
     2761 cagaagtact attccagtat ggacgattca aggcttgctt cacaaaccaa ggcaagtaat
     2821 agagattgga gtctctaaaa aggtagttcc cactgaatca aaggccatgg agtcaaagat
     2881 tcaaatagag gacctaacag aactcgccgt aaagactggc gaacagttca tacagagtct
     2941 cttacgactc aatgacaaga agaaaatctt cgtcaacatg gtggagcacg acacacttgt
     3001 ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg agacttttca
     3061 acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct gtcactttat
     3121 tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa
     3181 ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
     3241 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
     3301 tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag atagataatt
     3361 ctatataaac tctatcactg atagagtgga gagaacacgg gggactctag acaccatggt
     3421 gagtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
     3481 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
     3541 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caaccctggt
     3601 caccaccctg acctacgtaa gtttctgctt ctacctttga tatatatata ataattatca
     3661 ttaattagta gtaatataat atttcaaata tttttttcaa aataaaagaa tgtagtatat
     3721 agcaattgct tttctgtagt ttataagtgt gtatatttta atttataact tttctaatat
     3781 atgaccaaaa tttgttgatg tgcagggcgt gcagtgcttc tcccgttacc ctgatcatat
     3841 gaagcggcac gacttcttca agagcgccat gcctgaggga tacgtgcagg agaggaccat
     3901 cttcttcaag gacgacggga actacaagac acgtgctgaa gtcaagtttg agggtgacac
     3961 cctcgtcaac aggatcgagc ttaagggaat cgatttcaag gaggacggaa acatcctcgg
     4021 ccacaagttg gaatacaact acaactccca caacgtatac atcatggccg acaagcaaaa
     4081 gaacggcatc aaagccaact tcaagacccg ccacaacatc gaggacggcg gcgtgcaact
     4141 cgctgatcat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
     4201 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagatcacat
     4261 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
     4321 ataatgtcaa gccattctct cgcagatgat gttcactttg tgttttactt cctttatgca
     4381 ttcacagcaa taaaagaaag aaatctccat cgcttttggt tttcttctct gtcttaagtt
     4441 agtcgttttc gtgtctaatc tattacttat cattgtaata gactcttctt ctattgagat
     4501 ttgaatataa actaaaacac attccatttt actgtgttct caacattcag aatgcaaacg
     4561 gactaaccgt agtgactcgg ctttggctat tgttgttatc atcaaaatct atattgatac
     4621 tattatactc gatatgttat agactttatt aaaaaaaact ttgtttgttt gcagaaagtg
     4681 gatttttaat caaatttaat tgtgatgatt acaatgtcaa ttggttatgc aagatattaa
     4741 ttaatatgtt taagattttt cacttttaat aagtaaaata aaatacgtca aaaaaatttc
     4801 ctaaagataa tgccaaaaag gttaaattat agaaattaaa ctaaaatata ttaaaagttg
     4861 acttaaaaat gcaatgttga ccaccgacct aggggaaaac aaatttaccg tatctgggat
     4921 agaatctctt ctgtatcatc tcagataaag attactacat aaacacactt tacagtttta
     4981 cgacgcccaa aattcaattc ccggagatta aaaaccctaa aacaaacact ggcgattcga
     5041 ttttccctcc caattcaaat catctcttaa tcttcttctt ctcactatcc tctcaaattc
     5101 gccgccgatc ttccatcaat tttgatttct cgcaccgaaa tttcgacttt tcatctccta
     5161 tcgacgaaac cctaattttt cacactccag aaatcgaact ttgacgacga tgttaatggc
     5221 taaaccctgt ctggctattg ttgttatcat caaaatctat attgatacta ttatactcga
     5281 tatgttatag actttattaa aaaaaacttt gtttgtttgc agaaagtgga tttttaatca
     5341 aatttaattg tgatgattac aatgtcaatt ggttatgcaa gatattaatt aatatgttta
     5401 agatttttca cttttaataa gtaaaataaa atacgtcaaa aaaatttcct aaagataatg
     5461 ccaaaaaggt taaattatag aaattaaact aaaatatatt aaaagttgac ttaaaaatgc
     5521 aatgttgacc accgacctag gggaaaacaa atttaccgta tctgggatag aatctcttct
     5581 gtatcatctc agataaagat tactacataa acacacttta cagttttacg acgcccaaaa
     5641 ttcaattccc ggagattaaa aaccctaaaa caaacactgg cgattcgatt ttccctccca
     5701 attcaaatca tctcttaatc ttcttcttct cactatcctc tcaaattcgc cgccgatctt
     5761 ccatcaattt tgatttctcg caccgaaatt tcgacttttc atctcctatc gacgaaaccc
     5821 taatttttca cactccagaa atcgaacttt gacgacgatg ttaatggcta aacccgggca
     5881 tagaaaagtt ggctgcggcc gcgaatttct atcgatctat agataatgat ttctatcgat
     5941 ctatagataa tcatttctat cgatctatag ataatcattt ctatcgatct atagataatg
     6001 ttttctatcg atctatagat aatagtttct atcgatctat agataatcgg gtctaagcta
     6061 gctaggccac ctagcatgat acgaaacgta ccgtatcgtt aaggtaggtc acaatcccac
     6121 tatcctttcg caagaccctt cctctatata aggaagttca tttcatttgg agagaacacg
     6181 ggggactcta gagtaattga tacgaaacgt accgtatcgt taaggtcacc atgagccggc
     6241 tggacaagag caaagtgatc aacagcgccc tggaactgct caacgaagtg ggcatcgagg
     6301 gcctgaccac ccggaagctg gctcagaaac tgggcgtgga acagcccacc ctgtactggc
     6361 acgtgaagaa caagagagcc ctgctggacg ccctggccat cgagatgctg gaccggcacc
     6421 acacccactt ttgccccctg gaaggcgaga gctggcagga tttcctgcgg aacaacgcca
     6481 agagcttcag atgcgctctg ctgtcccacc gggatggcgc caaagtgcac ctgggcacca
     6541 gacctaccga gaagcagtac gagacactgg aaaaccagct ggccttcctg tgccagcagg
     6601 gcttcagcct ggaaaatgcc ctgtacgccc tgagcgccgt gggccacttt acactgggct
     6661 gcgtgctgga agatcaggaa caccaggtcg ccaaagagga aagagagaca cccaccaccg
     6721 acagcatgcc ccctctgctg agacaggcca ttgagctgtt cgatcatcag ggcgccgagc
     6781 ccgcctttct gttcggcctc gagctgatca tctgtggcct cgaaaaacag ctgaagtgcg
     6841 agtccggcag cccgaagaag aagaggaagg tttagtgtcg gtacgctgaa atcaccagtc
     6901 tctctctaca aatctatctc tctctatttt ctccataaat aatgtgtgag tagtttcccg
     6961 ataagggaaa ttagggttct tatagggttt cgctcatgtg ttgagcatat aagaaaccct
     7021 tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca
     7081 aaatccagta ctaaaatcca gatcgattac tcgcttcgag accgggctag agcggactag
     7141 ttctagagcg gccgccaccg cggtggagct ccagcttttg ttccctttag tgagggttaa
     7201 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     7261 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     7321 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     7381 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     7441 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     7501 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     7561 agaacatgaa ggccttgaca ggatatattg gcgggtaaac taagwcgctg tatgtgtttg
     7621 tttgagatct catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7681 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7741 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7801 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7861 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     7921 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7981 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8041 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8101 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     8161 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8221 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8281 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8341 ggattttggt catgcattct aggtactaaa acaattcatc cagtaaaata taatatttta
     8401 ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt
     8461 ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc
     8521 tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc
     8581 tgtcacccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa
     8641 aatcatacag ctcgcgcgga tctttaaatg gagtatcttc ttcccagttt tcgcaatcca
     8701 catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat
     8761 tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca
     8821 gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat
     8881 cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg
     8941 gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg
     9001 tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca
     9061 gcttatatac cttagcaggt gacattcctt ccgtatcttt tacgcagcgg tatttttcga
     9121 tcagtttttt caattccggt gatattctca ttttagccat ttattatttc cttcctcttt
     9181 tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact
     9241 gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa
     9301 gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat cacaggcagc
     9361 aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa
     9421 cccggcagct tattgtgcaa tgtaacatca gagattttga gacacaacgt ggctttgttg
     9481 aataaatcga acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga
     9541 ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct
     9601 catcaaccgt ggctccctca ctttctggct ggatgatggg gcgattcagg agatccccat
     9661 ccaacagccc gccgtcgagc gggctttttt atccccggaa gcctgtggat agagggtagt
     9721 tatccacgtg aaaccgctaa tgccccgcaa agccttgatt cacggggctt tccggcccgc
     9781 tccaaaaact atccacgtga aatcgctaat cagggtacgt gaaatcgcta atcggagtac
     9841 gtgaaatcgc taataaggtc acgtgaaatc gctaatcaaa aaggcacgtg agaacgctaa
     9901 tagccctttc agatcaacag cttgcaaaca cccctcgctc cggcaagtag ttacagcaag
     9961 tagtatgttc aattagcttt tcaattatga atatatatat caattattgg tcgcccttgg
    10021 cttgtggaca atgcgctacg cgcaccggct ccgcccgtgg acaaccgcaa gcggttgccc
    10081 accgtcgagc gcctttgccc acaacccggc ggccggccgc aacagatcgt tttataaatt
    10141 tttttttttg aaaaagaaaa agcccgaaag gcggcaacct ctcgggcttc tggatttccg
    10201 atccccggaa ttagactct
//
