LOCUS       sequence-393673-        9855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sth1Cas9-D9A-nickase_rAPOBEC1_cytosine base editor for RNA 
            recording.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   Jiao C, Reckstadt C, Konig F, Homberger C, Yu J, Vogel J, Westermann
            AJ, Sharma CM, Beisel CL
  TITLE     RNA recording in single bacterial cells using reprogrammed 
            tracrRNAs.
  JOURNAL   Nat Biotechnol. 2023 Jan 5. doi: 10.1038/s41587-022-01604-8.
  PUBMED    36604543
REFERENCE   2  (bases 1 to 9855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 5. doi: 10.1038/s41587-022-01604-8."
COMMENT     Sequence Label: Sth1Cas9n cytosine base editor
FEATURES             Location/Qualifiers
     source          1..9855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     386..405
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             complement(585..1463)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        complement(1502..1536)
                     /label=BBa_J23115
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.15"
     protein_bind    1592..1608
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1597..1619
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             1810..1839
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    2042..2058
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4862..4870
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6250..6498
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     terminator      6537..6608
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6624..6651
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(6928..8010)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     7972..7991
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8011..8088)
                     /gene="lacI"
                     /label=lacI promoter
     CDS             complement(8332..8991)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     8906..8925
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(8992..9094)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(9486..9503)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(9620..9855,1..310))
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(9700..9719)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag
       61 tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga
      121 ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga
      181 ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga
      241 aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa
      301 acgatctcaa gaagatcatc ttattaatca gataaaatat ttctaggctc atgagcccga
      361 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
      421 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatctgc tcatgtttga
      481 cagcttatca tcgatgcata atgtgcctgt caaatggacg aagcagggat tctgcaaacc
      541 ctatgctact ccgtcaagcc gtcaattgtc tgattcgtta ccaattatga caacttgacg
      601 gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg
      661 cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg
      721 gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc
      781 tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagatgtga cagacgcgac
      841 ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga
      901 tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg
      961 ttaatcgctt ccatgcgccg cagtaacaat tgctcaagca gatttatcgc cagcagctcc
     1021 gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc
     1081 ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta
     1141 agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga
     1201 gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc
     1261 ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga
     1321 ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg
     1381 gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg
     1441 ggatcatttt gcgcttcagc catacttttc atactcccgc cattcagaga agaaaccaat
     1501 tgctagcatt gtaccaaggg ctgagctagc tataaacttc ccaaccttac cagagggcgc
     1561 cccagctggc aattccgacg tctgtgtgga attgtgagcg gataacaatt tcacacaggg
     1621 ccctcggaca ccgaggagaa tgtcaagagg cgaacacaca acgtcttgga gcgccagagg
     1681 aggaacgagc taaaacggag cttttttgcc ctgcgtgacc agatcccgga gttggaaaac
     1741 aatgaaaagg cccccaaggt agttatcctt aaaaaagcca cagcatacat cctgtccgtc
     1801 caagcagagg agcaaaagct catttctgaa gaggacttgt tgcggaaacg acgagaacag
     1861 ttgaaacaca aacttgaaca gctacggaac tcttgtgcgt aaggaaaagt aaggaaaacg
     1921 attccttcta acagaaatgt cctgagcaat cacctatgaa ctgtcgcata gcatttttat
     1981 ccataagatt agcggatcct accttttact tgtgagcgct cacaattgat actgagcaca
     2041 attgtgagcg gataacaatt gcatgccact taaaaaggag atcaacaatg aaagcaattt
     2101 tcgtactgaa acatcttaat catgctatta aggaatacta gtatgtcaag tgagactggc
     2161 ccagtcgccg tagatcctac gctgcgccgt cgcattgagc ctcatgaatt tgaggtcttc
     2221 ttcgatcccc gcgagcttcg taaagaaacg tgcctgcttt atgagattaa ttggggtggc
     2281 cgtcattcta tctggcgcca tacgagtcag aacactaata agcatgttga ggtgaacttt
     2341 atcgaaaagt tcacgacgga acgtcatttc tgcccaaata cgcgttgctc tattacgtgg
     2401 tttttaagtt ggtcgccttg cggggagtgt tcacgtgcca tcactgagtt cctgtcacgt
     2461 tatccacatg tcaccttgtg catttatatt gcacgtcttt atcaccatga ggaccctcgc
     2521 aaccgccaag gattgcgtga tttgatcagc tctggagtca caattcaaat tatgactgag
     2581 caggagtctg gatattgttg gcgcaacttt gtgaattact caccttctaa tgaagcgaac
     2641 tggcctcgtt atcctcacct ttgggtacgc ctgtacgtgc ttgagttgta ctgcatcatc
     2701 cttggattgc caccgtgcct gaacatttta cgccgtaagc agccgcaact gacttcgttt
     2761 acaatcgccc ttcagtcgtg ccactaccaa cgcttacccc ctcacatcct ttgggcgacc
     2821 gggttgaagt cagggtcgga aactcctggt acgtcagaat ctgcgacccc ggaatcgagt
     2881 gacttagttt taggacttgc tatcggtata ggttctgttg gtgtgggtat ccttaacaaa
     2941 gtgacaggag aaattatcca taaaaactca cgcatcttcc cagcagctca agcggaaaat
     3001 aacctagtac gtagaacgaa tcgtcaagga agacgcttgg cacgacgtaa aaaacatcgt
     3061 agagttcgtt taaatcgtct atttgaggaa agtggattaa tcactgattt tacgaagatt
     3121 tcaattaatc ttaacccata tcaattacga gttaagggct tgaccgatga attgtctaat
     3181 gaagaactgt ttatcgctct taaaaatatg gtgaaacacc gtgggattag ttacctcgat
     3241 gatgctagtg atgacggaaa ttcatcagta ggagactatg cacaaattgt taaggaaaat
     3301 agtaaacaat tagaaactaa gacaccggga cagatacagt tggaacgcta ccaaacatat
     3361 ggtcaattac gtggtgattt tactgttgag aaagatggca aaaaacatcg cttgattaat
     3421 gtctttccaa catcagctta tcgttcagaa gccttaagga tactgcaaac tcaacaagaa
     3481 tttaatccac agattacaga tgaatttatt aatcgttatc tcgaaatttt aactggaaaa
     3541 cggaaatatt atcatggacc cggaaatgaa aagtcacgga ctgattatgg tcgttacaga
     3601 acgagtggag aaactttaga caatattttt ggaattctaa ttgggaaatg tacattttat
     3661 ccagacgagt ttagagcagc aaaagcttcc tacacggctc aagaattcaa tttgctaaat
     3721 gatttgaaca atctaacagt tcctactgaa accaaaaagt tgagcaaaga acagaagaat
     3781 caaatcatta attatgtcaa aaatgaaaag gcaatggggc cagcgaaact ttttaaatat
     3841 atcgctaagt tactttcttg tgatgttgca gatatcaagg gataccgtat cgacaaatca
     3901 ggtaaggctg agattcatac tttcgaagcc tatcgaaaaa tgaaaacgct tgaaacctta
     3961 gatattgagc aaatggatag agaaacgctt gataaattag cctatgtctt aacattaaac
     4021 actgagaggg aaggtattca agaagcctta gaacatgaat ttgctgatgg tagctttagc
     4081 cagaagcaag ttgacgaatt ggttcaattc cgcaaagcaa atagttccat ttttggaaaa
     4141 ggatggcata atttttctgt caaactgatg atggagttaa ttccagaatt gtatgagacg
     4201 tcagaagagc aaatgactat cctaacacga cttggaaaac aaaaaacgac ttcgtcttca
     4261 aataaaacaa aatatataga tgaaaaacta ttaactgaag aaatctataa tcctgttgtt
     4321 gctaagtctg ttcgccaggc tataaaaatc gtaaatgcgg cgattaaaga atacggagac
     4381 tttgacaata ttgtcatcga aatggctcgt gaaacaaatg aagatgatga aaagaaagct
     4441 attcaaaaga ttcaaaaagc caacaaagat gaaaaagatg cagcaatgct taaggctgct
     4501 aaccaatata atggaaaggc tgaattacca catagtgttt tccacggtca taagcaatta
     4561 gcgactaaaa tccgcctttg gcatcagcaa ggagaacgtt gcctttatac tggtaagaca
     4621 atctcaatcc atgatttgat aaataattct aatcagtttg aagtagatca tattttacct
     4681 ctttctatca cattcgatga tagccttgca aataaggttt tggtttatgc aactgctaac
     4741 caagaaaaag gacaacgaac accttatcag gctttagata gtatggatga tgcgtggtct
     4801 ttccgtgaat taaaagcttt tgtacgtgag tcaaaaacac tttcgaacaa gaaaaaagaa
     4861 tacctcctta cagaagaaga tatttcaaag tttgatgttc gaaagaaatt tattgaacga
     4921 aatcttgtag atacaagata cgcttcaaga gttgtcctca atgcccttca agaacacttt
     4981 agagctcaca agattgatac aaaagtttcc gtggttcgtg gccaatttac atctcaattg
     5041 agacgccatt ggggaattga gaagactcgt gatacttatc atcaccatgc tgtcgatgca
     5101 ttgattattg ccgcctcaag tcagttgaat ttgtggaaaa aacaaaagaa tacccttgta
     5161 agttattcag aagaccaact ccttgatatt gaaacaggtg aacttattag tgatgatgag
     5221 tacaaggaat ctgtgttcaa agccccttat caacattttg ttgatacatt gaagagtaaa
     5281 gaatttgaag acagtatctt attctcttat caagtggatt ctaagtttaa tcgtaaaata
     5341 tcagatgcca ctatttatgc gacaagacag gctaaagtag gaaaagataa ggcggatgaa
     5401 acttatgtct tagggaaaat caaagatatc tatactcagg atggttatga tgcctttatg
     5461 aagatttata agaaggataa gtcaaaattc ctcatgtatc gtcacgaccc acaaaccttt
     5521 gagaaagtta tcgagccaat tttagagaac tatcctaata agcaaataaa tgaaaaaggg
     5581 aaagaggtac catgtaatcc tttcctaaaa tataaagaag aacatggcta tattcgtaaa
     5641 tatagtaaaa aaggcaatgg tcctgaaatc aagagtctta aatactatga tagtaagcta
     5701 ggcaatcata ttgatattac tccgaaggat agtaacaata aagttgtctt acagtcagtt
     5761 tctccatgga gagcggatgt ctatttcaat aagactactg gaaaatacga aatcctcgga
     5821 ttgaaatatg ctgatttaca atttgaaaaa gggacaggaa catataagat ttcccaggaa
     5881 aaatacaatg acattaagaa aaaagagggt gtagattctg attcagaatt caagtttaca
     5941 ctttataaaa atgatttgtt actcgttaaa gatacagaaa caaaagaaca acagcttttc
     6001 cgttttcttt ctcgaactat gcctaaacaa aagcattatg ttgaattaaa accttatgat
     6061 aaacagaaat ttgaaggagg tgaggcgtta attaaagtgt tgggtaacgt tgctaatagt
     6121 ggtcaatgca aaaaagggct aggaaaatca aatatttcta tttataaggt aagaacagat
     6181 gtcctaggaa atcagcatat catcaaaaat gagggtgata agcctaagct agattttagc
     6241 ggaggttcca caaatctttc tgacattatt gaaaaagaaa ccggcaagca attggtgatt
     6301 caggaatcta ttcttatgct gccagaggag gtagaggagg tcattggaaa taaaccggag
     6361 tctgacatcc tggtgcatac ggcctatgac gagtccacag acgagaacgt catgctgctt
     6421 acatcggatg cccctgagta taagccctgg gctcttgtca ttcaggacag taatggcgaa
     6481 aataagatta agatgcttag cggggggtcc taactcgagt aaggatctcc aggcatcaaa
     6541 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     6601 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatacct
     6661 agggagacgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag
     6721 cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc
     6781 atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag
     6841 gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg
     6901 agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6961 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     7021 cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg
     7081 gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg
     7141 tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac
     7201 taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag
     7261 cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg
     7321 catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg
     7381 aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga
     7441 acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac
     7501 gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga
     7561 gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg
     7621 gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac
     7681 cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc
     7741 cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag
     7801 actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg
     7861 gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga
     7921 aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc
     7981 tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg
     8041 gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac
     8101 gctctccctt atgcgactcc tgcattagga agaagcacac ggtcacactg cttccggtag
     8161 tcaataaacc ggtaaaccag caatagacat aagcggctat tcaacgaccc tgccctgaac
     8221 cgacgaccgg gtcgaatttg ctttcgaatt tctgccattc atccgcttat tatcacttat
     8281 tcaggcgtag caaccaggcg tttaagggca ccaataactg ccttaaaaaa attacgcccc
     8341 gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc
     8401 atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt
     8461 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa
     8521 atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa
     8581 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg
     8641 tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg
     8701 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt
     8761 cattgccata cggaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc
     8821 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg
     8881 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg
     8941 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc
     9001 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt
     9061 atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc aaaagttggc
     9121 ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga agtgatcttc
     9181 cgtcacaggt atttattcgg cgcaaagtgc gtcgggtgat gctgccaact tactgattta
     9241 gtgtatgatg gtgtttttga ggtgctccag tggcttctgt ttctatcagc tgtccctcct
     9301 gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat cagcgctagc
     9361 ggagtgtata ctggcttact atgttggcac tgatgagggt gtcagtgaag tgcttcatgt
     9421 ggcaggagaa aaaaggctgc accggtgcgt cagcagaata tgtgatacag gatatattcc
     9481 gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct
     9541 tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag ggaagtgaga
     9601 gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat cacgaaatct
     9661 gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     9721 ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc
     9781 tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc
     9841 tccaagctgg actgt
//
