LOCUS       sequence-393675-        2693 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Amino acids 25-308 of SUL-I with an N-terminal 6xHis-tag followed by
            a TEV site in the pJL1 vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Warfel KF, Laigre E, Sobol SE, Gillon E, Varrot A, Renaudet O, Dejeu
            J, Jewett MC, Imberty A
  TITLE     Cell-free expression and characterization of multivalent 
            rhamnose-binding lectins using biolayer interferometry.
  JOURNAL   Glycobiology. 2023 Mar 7:cwad018. doi: 10.1093/glycob/cwad018.
  PUBMED    36882003
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Glycobiology. 2023 Mar 7:cwad018. doi: 10.1093/glycob/cwad018."
COMMENT     Sequence Label: pJL1_SUL-I
FEATURES             Location/Qualifiers
     source          1..2693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..60
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(982..1000)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      996..1043
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1061..1876)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1784..1803
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1868..1887
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1998..2586
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2487..2506
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2614..2633
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2614..2632
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2664..2686
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 atgggtagca gccatcacca tcatcatcat agcagcggtg aaaatctgta ttttcagagc
       61 tccatgggcg cagttggtcg tacctgtgaa ggtaaaagcc tggatctgga atgtccggaa
      121 ggttatatca ttagcgtgaa ctatgcaaac tatggtcgta atagtccggg tatttgcccg
      181 cataaaagca gcaatgcacc gccttgtagc gcaagcagca gcctgcgtat tatcaatgaa
      241 cattgcgatg gtcgtagcag ctgtagcgtt catgcaacca atgatgtttt tggtgatccg
      301 tgtcgtggtg tgtataaata cctggaagtt gattatagct gccgtcgtga tccggattgt
      361 cagcgcgaac tggattgcga aggtaatagc attaatatgc tgtgtccgta tgcagaaaca
      421 ccggcaattc atatttgcta tgccatgtat ggtcgtcaga ccagcgaacc ggtttgtccg
      481 agcaaaagca ttagcaccac caattgtgca gcaagcagct cactgagcac cgcacgtagc
      541 gtttgtgaag gtcgttcaga atgtagcatt gcagcaagta acgatgtgtt tggcgatcct
      601 tgtattggca cctacaaata tctggaaatc gattacattt gtgcacgtcg tggtcgttca
      661 tgcgaaggtt caagtctgac cctgagctgt agcagcggtc agaccattag cgttctggat
      721 gcattttatg gtcgcaccgc aggtccggaa atttgtaaag gtaatgcaca ggatcagaat
      781 tgccgtgccg aaagcagcct gaatattgtt cagagcgcct gtaatggtcg cagctcatgc
      841 agcgttaatg caaataacaa cgtgttcggt gatccatgtg ttgggacata taagtatctg
      901 gaagtgctgt ataaatgcgc ctaactcgag gtcgaccggc tgctaacaaa gcccgaaagg
      961 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
     1021 aacgggtctt gaggggtttt ttgctgaaag ccaattctga ttagaaaaac tcatcgagca
     1081 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
     1141 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
     1201 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
     1261 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
     1321 aaagcttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
     1381 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata
     1441 cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
     1501 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
     1561 ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
     1621 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
     1681 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
     1741 tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
     1801 acccatataa atcagcatcc atgttggaat ttaatcgcgg cttcgagcaa gacgtttccc
     1861 gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg
     1921 ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga gacacaacgt
     1981 gagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2041 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2101 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     2161 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2221 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2281 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2341 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     2401 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2461 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2521 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2581 tggaaaaacg ccagcaacgc gatcccgcga aattaatacg actcactata gggagaccac
     2641 aacggtttcc ctctagaaat aattttgttt aactttaaga aggagatata cat
//
