LOCUS       sequence-393682-        2426 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Amino acids 1-195 of CSL3 with an N-terminal 6xHis-tag followed by a
            TEV site in the pJL1 vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Warfel KF, Laigre E, Sobol SE, Gillon E, Varrot A, Renaudet O, Dejeu
            J, Jewett MC, Imberty A
  TITLE     Cell-free expression and characterization of multivalent 
            rhamnose-binding lectins using biolayer interferometry.
  JOURNAL   Glycobiology. 2023 Mar 7:cwad018. doi: 10.1093/glycob/cwad018.
  PUBMED    36882003
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Glycobiology. 2023 Mar 7:cwad018. doi: 10.1093/glycob/cwad018."
COMMENT     Sequence Label: pJL1_CSL3
FEATURES             Location/Qualifiers
     source          1..2426
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..60
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(715..733)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      729..776
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(794..1609)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1517..1536
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1601..1620
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1731..2319
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2220..2239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2347..2366
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2347..2365
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             2397..2419
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 atgggtagca gccatcacca tcatcatcat agcagcggtg aaaatctgta ttttcagagc
       61 tccatgggcg cgatctcaat cacatgcgag ggatctgacg ctcttcttca atgtgacggc
      121 gctaagatcc acataaagcg agcaaactac ggacggagac aacacgacgt gtgttcaatc
      181 ggccgccccg acaaccaatt aacagacaca aactgtctct ctcaatcgag tacatctaag
      241 atggcggagc gatgcggcgg caagtctgag tgcatcgtac cagctagtaa cttcgtcttc
      301 ggagacccct gcgtgggaac ttacaagtat cttgacacaa agtactcatg tgtacaacaa
      361 caggagacga tctctagtat catctgtgag gggtcagact cacaactctt atgtgacaga
      421 ggcgagatca gaatccaacg cgccaattac ggccgacgtc agcatgacgt ttgctccatc
      481 gggcgcccgc accaacaact taagaacacg aactgtcttt ctcaaagtac gacatctaag
      541 atggcagaac gttgcgacgg gaagcgccaa tgcatagtct ccgtatctaa cagtgtattc
      601 ggtgacccat gtgtaggcac ctataaatac ttggacgtgg cttacacttg tgactaactc
      661 gaggtcgacc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
      721 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
      781 aagccaattc tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
      841 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
      901 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
      961 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
     1021 catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt
     1081 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
     1141 tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac
     1201 aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac
     1261 ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga
     1321 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
     1381 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
     1441 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac
     1501 ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg
     1561 aatttaatcg cggcttcgag caagacgttt cccgttgaat atggctcata acaccccttg
     1621 tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg
     1681 caatgtaaca tcagagattt tgagacacaa cgtgagatca aaggatcttc ttgagatcct
     1741 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     1801 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     1861 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     1921 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     1981 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2041 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     2101 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     2161 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     2221 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     2281 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcgatcccg
     2341 cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg
     2401 tttaacttta agaaggagat atacat
//
