LOCUS       sequence-393683-       10582 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express tKiMBImut(AA), AkaLuc and Blasticidin in a lentivirus 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10582)
  AUTHORS   Wu Y, Walker JR, Westberg M, Ning L, Monje M, Kirkland TA, Lin MZ, 
            Su Y
  TITLE     Kinase-Modulated Bioluminescent Indicators Enable Noninvasive 
            Imaging of Drug Activity in the Brain.
  JOURNAL   ACS Cent Sci. 2023 Mar 20;9(4):719-732. doi: 
            10.1021/acscentsci.3c00074. eCollection 2023 Apr 26.
  PUBMED    37122464
REFERENCE   2  (bases 1 to 10582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscentsci.3c00074"; journalName: "ACS Cent Sci"; date: 
            "2023-03-20- 20"; volume: "9"; issue: "4"; pages: "719-732"
COMMENT     Sequence Label: pLL3.7m_tKiMBImut-T2A-AkaLuc-P2A-Blasticidin
FEATURES             Location/Qualifiers
     source          1..10582
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        63..366
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        368..566
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             584..764
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    811..936
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1433..1666
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1851..1895
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2193..2310
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2369..2681
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     2600..2620
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     2698..2714
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2756..2789)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     enhancer        2905..3208
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3209..3412
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      3441..3450
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5568..5621
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7281..7337
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7338..7736
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    7758..8346
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7811..7831)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8229..8240)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8349..8365)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             8588..8768
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(8830..9418)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8910..8929)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9589..10380,10381..10449))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10212..10231
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10450..10554)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
       61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      361 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      421 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      481 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      541 gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga
      601 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
      661 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
      721 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
      781 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
      841 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
      901 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
      961 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     1021 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     1081 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     1141 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     1201 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     1261 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     1321 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1381 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1441 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1501 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1561 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1621 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1681 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1741 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1801 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1861 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1921 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1981 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     2041 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     2101 gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa
     2161 ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     2221 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     2281 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
     2341 gtttggttag taccgggccc gctctagaga tccgacgcgc catctctagg cccgcgccgg
     2401 ccccctcgca cagacttgtg ggagaagctc ggctactccc ctgccccggt taatttgcat
     2461 ataatatttc ctagtaacta tagaggctta atgtgcgata aaagacagat aatctgttct
     2521 ttttaatact agctacattt tacatgatag gcttggattt ctataagaga tacaaatact
     2581 aaattattat tttaaaaaac agcacaaaag gaaactcacc ctaactgtaa agtaattgtg
     2641 tgttttgaga ctataaatat cccttggaga aaagccttgt taacgcgcgg tgaccctcga
     2701 ggtcgacggt atcgataagc tcgcttcacg agattccagc aggtcgaggg acctaataac
     2761 ttcgtatagc atacattata cgaagttata ttaagggttc caagcttaag cggccgcgtg
     2821 gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt
     2881 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     2941 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     3001 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     3061 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     3121 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     3181 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     3241 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     3301 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     3361 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt
     3421 gaaccgtcag atccgctagc gccaccatgg tgagcaaggg cgaggaggtc atcaaagagt
     3481 tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc gagatcgagg
     3541 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
     3601 gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac ggctccaagg
     3661 cgtacgtgaa gcaccccgcc gacatccccg attacaagaa gctgtccttc cccgagggct
     3721 tcaagtggga gcgcgtgatg aacttcgagg acggcggtct ggtgaccgtg acccaggact
     3781 cctccctgca ggacggcacg ctgatctaca aggtgaagat gcgcggcacc aacttccccc
     3841 ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt
     3901 acccccgcga cggcgtgctg aagggcgaga tccaccaggc cctgaagctg aaggacggcg
     3961 gccactacct ggtggagttc aagaccatct acatggccaa gaagcccgtg caactgcccg
     4021 gctactacta cgtggacacc aagctggaca tcacctccca caacgaggac tacaccatcg
     4081 tggaacagta cgagcgctcc gagggccgcc accacctgtt cctggggcat ggcaccggca
     4141 gcaccggcag cggcagctcc ggcaccgcct cctccgagga caacaacatg gccgtcatca
     4201 aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga
     4261 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
     4321 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct
     4381 ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg
     4441 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc
     4501 aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact
     4561 tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc
     4621 gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg
     4681 acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac
     4741 tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca
     4801 ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttccgg ctggaagatt
     4861 tcgttgggga ctgggaacag acagccgcct acaacctgga ccaagtcctt gaacagggag
     4921 gtgtgtccag tttgctgcag aatctcgccg tgtccgtaac tccgatccaa aggattgtcc
     4981 ggagcggtga aaatgccctg aagatcgaca tccatgtcat catcccgtat gaaggtctga
     5041 gcgccgacca aatggcccag atcgaagagg tgtttaaggt ggtgtaccct gtggatgatc
     5101 atcactttaa ggtgatcctg ccctatggca cactggtaat cgacggggtt acgccgaaca
     5161 tgctgaacta tttcggacgg ccgtatgaag gcatcgccgt gttcgacggc aaaaagatca
     5221 ctgtaacagg gaccctgtgg aacggcaaca aaattatcga cgagcgcctg atcacccccg
     5281 acggctccat gctgttccga gtaaccatca acagcatcat ggcggacgag gagaagctgc
     5341 cgcccggctg ggagaagcgc atgagccgca gctcaggccg agtgtactac ttcaaccaca
     5401 tcactaacgc cagccagtgg gagcggccca gcggcaacag cagcagtggt ggcaaaaacg
     5461 ggcaggggga gcctgccagg gtgaccggct accggctgtt cgagccagat gcccctagag
     5521 ccccagtgga taaagcaaag ctgtcattcc aattccccgg atccggtgaa ggtcgaggct
     5581 ccctcctcac ttgtggggat gtggaagaaa acccagggcc tatggaagat gccaaaaaca
     5641 ttaagaaggg cccagcgccg ttctacccac tcgaagacgg gaccgccggc gagcagctgc
     5701 acaaagccat gaagcgctac gccctggtgc ccggcgccat cgcctttacc gacgcacata
     5761 ttcaggtgga cgttacctac gccgagtact tcgagatgag cgttcggctg gcagaagcta
     5821 tgaggcgcta tgggctgaat acaaaccatc ggatcgtggt gtgcagcgag aatagctcgc
     5881 agttcttcat gcccgtgttg ggtgccctgt tcatcggtgt ggctgtggcc ccagctaacg
     5941 acatctacaa cgagcgcgag ctgctgaaca gcatgggcat cagccagccc accgtcgtat
     6001 tcgtgagcaa gaaagggctg cgaaaggtcc tcaacgtgca aaagaagcta ccgatcatac
     6061 gaaagatcat catcatggat agcaagaccg actaccaggg cttccaaagc atgtacacct
     6121 tcgtgacttc ccatttgcca cccagcttca acgagtacga cttcgtgccc gagagcttcg
     6181 accgggacaa aaccatcgcc ctgatcatga acagtagtgg tagtacagga ttacccaagg
     6241 gcgtagccct accgcaccgc accgcttgtg tccgattcag tcatgcccgc gaccccatct
     6301 tcggctacca gaacatcccc gacaccgcta tcctcagcgt ggtgccattt caccacggct
     6361 tcggcatgtt caccacgctg ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc
     6421 gcttcgagga ggagctattc ttgcgcagct tgcaagacta taagattcaa tctgccctgc
     6481 tggtgcccac actatttagc tgcctcgcta agagcactct catcgacaag tacgacctaa
     6541 gcagcttgcg cgagatcgcc agcggcgggg cgccgctcag caaggaggta ggtgaggccg
     6601 tggccaaacg cttccgccta ccaggcatcc gccagggcta tggcctgaca gaaacaacca
     6661 acgccgtcat gatcaccccc gagggggacc gtaagcctgg ctcagtaggc aaggtggtgc
     6721 ccttcttcga ggctaaggtg gtagacttgg tcaccggtaa gacactgggt gtgaaccagc
     6781 gcggtgagct gtgcgtccgt ggccccatga tcatgagcgg ctacgttaac aaccccgagg
     6841 ctacgaacgc tctcatcgac aaggacggct ggctgcacag cggcgacatc gcctactggg
     6901 acgaggacga gcacttcttc atcgtggacc ggctgaagag cctgatcaaa tacaagggct
     6961 accaggtagc cccagccgaa ctggagggca tcctgctgca acacccctac atcttcgacg
     7021 ccggagtcgc cggcctgccc gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgt
     7081 tggaacacgg taaaaccatg accgagaaag agatcgtgga ctatgtggcc agccaggtta
     7141 caaccgccaa gaagctgcgc ggtggtgttg tgtttgtgga tgaagtccct agaggatcga
     7201 ccggcaagtt agacgcccgc aagatccgcg agattctcac taaggccaag aaggacggca
     7261 agatcgccgt gggctcaggt gccaccaact tcagcctgct gaagcaggca ggcgacgtgg
     7321 aagagaaccc tggccctatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa
     7381 gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag
     7441 ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac
     7501 cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt
     7561 gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac
     7621 aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc
     7681 cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaataag
     7741 atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     7801 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     7861 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     7921 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     7981 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     8041 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     8101 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     8161 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     8221 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     8281 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     8341 ccccgcatcg ataccgtcga cctcgatcga gacctagaaa aacatggagc aatcacaagt
     8401 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     8461 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgac
     8521 gcgtgatttt aaaagaaaag gggggactgg aagggctaat tcactcccac tgctttttgc
     8581 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     8641 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     8701 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     8761 ctctagcagc atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8821 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8881 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8941 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9001 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9061 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9121 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9181 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9241 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     9301 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9361 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9421 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9481 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     9541 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     9601 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9661 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9721 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9781 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9841 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9901 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9961 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    10021 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    10081 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    10141 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    10201 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    10261 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    10321 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    10381 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    10441 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    10501 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    10561 tccccgaaaa gtgccacctg ac
//
