LOCUS       sequence-393685-       12151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for the expression of er-mCherry (SP-mCherry-HDEL) in 
            plants (for Agrobacterium-mediated genetic transformation and 
            visualization of endoplasmic reticulum).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12151)
  AUTHORS   Ishikawa K, Konno R, Hirano S, Fujii Y, Fujiwara M, Fukao Y, Kodama 
            Y
  TITLE     The ER membrane-bending protein RETICULON facilitates chloroplast 
            relocation movement in Marchantia polymorpha.
  JOURNAL   Plant J. 2022 Apr 27. doi: 10.1111/tpj.15787.
  PUBMED    35476214
REFERENCE   2  (bases 1 to 12151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2022 Apr 27. doi: 10.1111/tpj.15787."
COMMENT     Sequence Label: pMpGWB302-er-mCherry
FEATURES             Location/Qualifiers
     source          1..12151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1179..1203
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1281..1988
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1694..1713
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(2005..2029)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      2061..2313
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2346..2362)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2346..2362)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2359..2381)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2370..2386
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2394..2400,2401..2418,2419..2424))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2439..2460
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    2545..2719
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(4802..5478)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     misc_feature    5673..5697
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6218..7009
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7255..7843
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7744..7763
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7997..8014
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8029..8169
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8095..8114)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8255..8277
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8513..8707
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8773..9846)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10275..10904)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagcag
     1201 gcttcatggc cagactcaca agcatcattg ccctcttcgc agtggctctg ctggttgcag
     1261 atgcgtacgc ctaccgcaca atggtgagca agggcgagga ggataacatg gccatcatca
     1321 aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga
     1381 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
     1441 ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct
     1501 ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg
     1561 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc
     1621 aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact
     1681 tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc
     1741 ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg
     1801 acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc
     1861 tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca
     1921 ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc
     1981 tgtacaagca cgacgagctc taggacccag ctttcttgta caaagtggtt gataacagcg
     2041 cttagagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga
     2101 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
     2161 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
     2221 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
     2281 tatcgcgcgc ggtgtcatct atgttactag atcgggaatt ggttccggaa ccaattcgta
     2341 atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     2401 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     2461 ggctgaatta ggcgcgccta tttctgaatt caacacattg cggacgtttt taatgtactg
     2521 aattaacgcc gaattaattc gggggatctg gattttagta ctggattttg gttttaggaa
     2581 ttagaaattt tattgataga agtattttac aaatacaaat acatactaag ggtttcttat
     2641 atgctcaaca catgagcgaa accctatagg aaccctaatt cccttatctg ggaactactc
     2701 acacattatt atggagaaac tcgagttagt atatagtcct tccatcaccc tctgtgataa
     2761 tctccttaaa acttgctccc ccaggaatca taggcagaac gtgctcctgg tggggaacta
     2821 tcacatccag taaataagga ccgggagaat ctagcatagt ttgaattgct gctcttagtt
     2881 catccttctt caccacacgc gcatttggca ccttgaaacc gtctgcaact gtacagaagt
     2941 cggggtagat ctgctcctcg tgatctggat cacctaagta agtatgtgct ctgttggcct
     3001 tgtagaatct atcctccaat tgtacgacca ttcctagatg ttggttgttt aagatcatga
     3061 tcttcacggg taacttctcc acaaaaattg tagccaactc ctgtacgttc atcacaaagc
     3121 taccatcgcc atcgatgtcg atcacaggaa tatctggttg agttgcggca gcccccaaag
     3181 cagaaggaag tccgaatccc atggctccaa gacctccaga tgtcaaccaa cgccgcggtt
     3241 cgttgtactt gaaccactgg gctgcccaca tttgatgttg gccaacacca gtagatatga
     3301 tggcatttcc accagtgagt tcgtacagcg tctgtatggc agattgagga gcaatggcat
     3361 cctcgaatac gggaaacttc attggccatt tctgcttttg ttcttctagt tctgccaccc
     3421 agttggagaa gtcgaaagtc ctcttttgac tcttcaagag ggcattgatt tcacttagcg
     3481 caggtttcac atcagcacac atggacacat ggggctgttt gttctttcca atttcagcag
     3541 ggtcaatatc gatatgaacg atgctggacc tgctagcaaa agtctccagt ttaccagtca
     3601 ctcgatcgtc gaaacgcact ccaaatgcca acaacagatc tgcttggtcc acggcgtagt
     3661 tggcgtaaac agttccatgc attccaagca ttttcaaact taatggatca gactcgggat
     3721 acgctcccaa acccattaaa gtcgatgtga taggaattcc ggtaagctcg acaaattccc
     3781 tcaactcttc gctggcgtga ttgcaacctc ctccagaata cactacaggc ttcttagaac
     3841 tagaaatgag tcttataatc tgctcgatta acgttggttc cggtggccga ggcagtcgtt
     3901 gtaagtagcc cttcagttgc attggctgag accaatctgg aatagccatt tgctgttgca
     3961 cgtccttggg aatatctacc aagactgggc ccggcctacc tgaagctgcc aaaaagaaag
     4021 cctccctgat tacgcgaggg atgtcatcaa tgttcataac aaggtaattg tgcttggtga
     4081 tcgatctcgt aacctccaca attggagttt cttgaaaagc atcggtacca atcatcctac
     4141 tggatacttg acctgtaatg gccactagtg gcacactgtc caagagggca tcagcgagcc
     4201 cggtaacaag attggtcgct ccaggaccgg atgtggctat acaaacccct actcgtccag
     4261 acgatttagc ataaccttca gcagcgaaaa tctccccctg ctcgtggcga cagagaatgt
     4321 tgcggatgct gctggaacgt gtaagagcct ggtgaatctc catagacgct ccgccgggat
     4381 aagcgaacac cgttctaatc ccttctcgct caagagcctc caccaaaatg tcggctcctt
     4441 tgcggggctc gtcatccttg tagcgagact gaaagtcgga cggggttggt gcaacgagtg
     4501 acttcccgct gctaagccgt ggtccactca catcgactgc agactggatc ttctcgctaa
     4561 cagcttgggc tttcacttcc agtgaacgct cagtcttccg agaggtgttg tatgcatgtc
     4621 ccctcagagc ggctcccgag aagaaaccac tcctcacctt tagctcttta gctcccttgc
     4681 aacaagacct tgccgatggc tgcaccttct ccaggctcgc tatgctcaaa ggcacagctg
     4741 cgcatccact aattgacgcc atgctcgaga gagatagatt tgtagagaga gactggtgat
     4801 ttcagcgtgt cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag
     4861 gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct
     4921 ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat
     4981 ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaattat
     5041 ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg
     5101 ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc
     5161 tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca
     5221 ccatgttcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg
     5281 atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg
     5341 atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc
     5401 cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc
     5461 ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg
     5521 gagtagacga gagtgtcgtg ctccaccatg ttggcaagct gctctagcca atacgcaaac
     5581 cgaattcaga aataaattca gcctaattcg gcgttaattc agtacattaa aaacgtccgc
     5641 aatgtgttat taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca
     5701 gccagccaac agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc
     5761 ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg
     5821 ttaccgatgc tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct
     5881 cgcggagggt agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaattcg
     5941 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     6001 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     6061 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     6121 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     6181 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga
     6241 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
     6301 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
     6361 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
     6421 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
     6481 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
     6541 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
     6601 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
     6661 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
     6721 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
     6781 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
     6841 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
     6901 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     6961 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctagctag
     7021 aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta
     7081 agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg
     7141 tgcataataa gccctacaca aattgggaga tatatcatgc atgaccaaaa tcccttaacg
     7201 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     7261 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     7321 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     7381 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     7441 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     7501 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     7561 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     7621 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     7681 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     7741 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     7801 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     7861 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     7921 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     7981 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     8041 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     8101 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     8161 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     8221 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     8281 tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag
     8341 tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt
     8401 tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga
     8461 ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca
     8521 ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc
     8581 ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg
     8641 gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct
     8701 atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt gccctagcat
     8761 ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat
     8821 gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt
     8881 tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact
     8941 gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc
     9001 caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt
     9061 cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat
     9121 ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc
     9181 ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc
     9241 aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc
     9301 atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg
     9361 cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat
     9421 cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac
     9481 gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag
     9541 acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat
     9601 cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc
     9661 ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc
     9721 accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt
     9781 ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc
     9841 gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc
     9901 cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg
     9961 gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc
    10021 atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt
    10081 aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa
    10141 ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt
    10201 gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc
    10261 tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg
    10321 cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct
    10381 gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc
    10441 gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc
    10501 gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg
    10561 gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg
    10621 atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc
    10681 cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc
    10741 ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact
    10801 aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc
    10861 ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta
    10921 tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact
    10981 caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg
    11041 gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg
    11101 agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt
    11161 cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg
    11221 gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc
    11281 accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag
    11341 ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc
    11401 gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt
    11461 cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg
    11521 ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt
    11581 gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc
    11641 aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac
    11701 gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc
    11761 ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt
    11821 ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct
    11881 cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa
    11941 acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga
    12001 cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact
    12061 atagcagcgg aggggttgga tcaaagtact ttgatcccga ggggaaccct gtggttggca
    12121 tgcacataca aatggacgaa cggataaacc t
//
