LOCUS       sequence-393698-       11992 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for the expression of Citrine-NLS with MpIGPDm 
            selective marker in Marchantia polymorpha (igpd mutants) ..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11992)
  AUTHORS   Fukushima T, Kodama Y
  TITLE     Selection of a histidine auxotrophic Marchantia polymorpha strain 
            with an auxotrophic selective marker.
  JOURNAL   Plant Biotechnol (Tokyo). 2022 Dec 25;39(4):345-354. doi: 
            10.5511/plantbiotechnology.22.0810a. Epub 2022 Nov 26.
  PUBMED    37283617
REFERENCE   2  (bases 1 to 11992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.5511/plantbiotechnology.22.0810a"; journalName: "Plant 
            Biotechnol (Tokyo)"; date: "2022-12-25- 25"; volume: "39"; issue: 
            "4"; pages: "345-354"
COMMENT     Sequence Label: pMpGWBhis03-Citrine-NLS
FEATURES             Location/Qualifiers
     source          1..11992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2057..2081
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(2084..2086,2087..2089,2090..2602)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(2129..2150)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2390..2409)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2737..2758
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2789..2809
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(2814..2838)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      2870..3122
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3155..3171)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3155..3171)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3168..3190)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3179..3195
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3203..3209,3210..3227,3228..3233))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3248..3269
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    3354..3528
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(4643..5319)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(4686..4706)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    5514..5538
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6059..6850
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7096..7684
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7585..7604
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7838..7855
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7870..8010
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7936..7955)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8096..8118
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8354..8548
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8614..9687)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10116..10745)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttggcc cgggcaaatg agtcacacac attgttgaga gacatatcaa aataactcaa
      361 ttgtaaggta agttgataga tttcccagtt aaatgcatta ttttaacaat atttgtatct
      421 caaattttca aaatcatatc aaatttgcag atcttgatga tgattcacaa ctctgtcttc
      481 gatttttgat tgttaacggg tatcgatatt tgggtaactt aaaaagaatt tgctaaagat
      541 cttaaaaaac agattaaaaa aacctttcaa aatagctaat cctttaaatc cgtttcacaa
      601 tataatgctc attgatgata atcgaaaaga ttccatttta aagtgcatat aaattgctta
      661 attatctctt tctgtctcgt aatattttgt tctctcctat tggatcggac ccgcatcttc
      721 tcgatcagtt cactatacta atatttggtc aacttcaaga gagattagaa cttcgttctc
      781 caaagtgtaa aggccgacaa gcagaaaagg aaacttcaat cagaagcctt ctaggttttc
      841 ccgcgtccca aaaagcgaag ggaggacggg gaggacggtg aggccagaag aagaaagcaa
      901 cagtaaaatc tcatgtgatt gcgtgcatgt catggatcta tgggatgcta gtccaccgtt
      961 tggaagtatg tgcagaagga gaaaggcgcg ggaggccaaa agcgacttcg gccacctctc
     1021 tactttctag aaagtttgct cgcagcttgt aataagcgcg gaaaaggtac tcgaaaaacc
     1081 ggagcaatcg gtgtggtatc agcagtcggg ttcgctaaac ctacgtcttc ttattttgga
     1141 atgttaaatt gaaagggcgt gccggcccta agcacggacg atgtgacaga cggaggagtg
     1201 tgagcaattc ggatgacgat gctggccggc agtcacgaca aagagtccga aggggcatgg
     1261 acagggcaat atataaatgg aaaagaggct ttgaatattc ctaacccttc gtcgtcggcc
     1321 tcccaacttg ccctcgcatg ctgaattggc cgcctatctg cgaatggccg gccgccgacc
     1381 ctaagtcgca ataacttgaa cggagccgat gcaaagtcct tatcgaacgc tcgttattat
     1441 aaacggggca gacctaggta tttaattata tcaaaaccct ccgcaatttc gctaatcatt
     1501 ccctaatttc ttctactttg gcgtctagtg ctgtggtcgc gcttgctctc aaagctcgct
     1561 gggaggcagt ggagcgtctg gcttaaaatc gatcgccgca accctagagt tcctttcact
     1621 tctttctctc ttgcgtggag agcatcgaca tcgaagagct gcatcttgtc ttctggtatg
     1681 cgccttgtgc cctcgtctct gtacgtcgtt acgattgctc gatattgtga ttgtctagac
     1741 ctgaaacccg actggagctt ttacgtggtg tcgattgggg cctcgagcga gtgatttttt
     1801 aggttcgttt tagccaagta catcccactt tggtcagtcc tgttatctcg gtaaggtgtt
     1861 cagttcggtc tctggtgcgg aggtgcctcg taagtttcct gcatgataga tttgtctatc
     1921 cttgacttga agaggagtcg aaggtcagcg aggttggctg tgtgctatgg acgtcggtgc
     1981 tccttggttc cgtgcttcta tgggtgttgc ctcaacagta ttgatgtgcc tgcagaaagg
     2041 ttgactagag ttatcaacaa gtttgtacaa aaaagcaggc tccatggtga gcaagggcga
     2101 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     2161 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     2221 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg
     2281 ctacggcctg atgtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
     2341 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     2401 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     2461 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     2521 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     2581 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     2641 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc
     2701 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     2761 cgccgccggg accactttgt acaagcctcc taagaagaag cgcaaggtgt gagacccagc
     2821 tttcttgtac aaagtggttg ataacagcgc ttagagctcg aatttccccg atcgttcaaa
     2881 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     2941 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     3001 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     3061 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     3121 tcgggaattg gttccggaac caattcgtaa tcatgtcata gctgtttcct gtgtgaaatt
     3181 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     3241 gtgcctaatg agtgagctaa ctcacattag gctgaattag gcgcgcctat ttctgaattc
     3301 aacacattgc ggacgttttt aatgtactga attaacgccg aattaattcg ggggatctgg
     3361 attttagtac tggattttgg ttttaggaat tagaaatttt attgatagaa gtattttaca
     3421 aatacaaata catactaagg gtttcttata tgctcaacac atgagcgaaa ccctatagga
     3481 accctaattc ccttatctgg gaactactca cacattatta tggagaaact cgagcttgtc
     3541 gatcgacaga tcccggtcgg catctacttc atgcgcgaga taagaccccc ttagagcttg
     3601 gaacggatcc ctttcttctc gtatcatatt caagcgcctg tcgcagagct ctggcaaaag
     3661 ctttgaaagt agcctcgatg atgtggtgag aattggttcc cgcaagcttg cgaatatgga
     3721 gggtcattcc agacgtgttc accaccgatt ggaaaaaatg ctccaccaac tgtgtatcgt
     3781 aggtaccaac tcgttgagtg ggaatgtcta cattataccc caaaaatggt cttcctgata
     3841 aatccaaaac aacatggatc agagcttcat caagtggagc cgaaaaatcc ccaaagcgat
     3901 atatgccctt cctatctcct aaggcctgga gtaatgcagt tccgacagcc aaaccaatgt
     3961 cctcgttcgt gtgatgatca tcaatgtgaa tgtcgccctt tgccttcact gtcacatcta
     4021 acagtccatg cgacgcaagt tgatctagca tgtgatcgag aaagggtatg ccggtgtcac
     4081 agtcgcagat tcctgtcccg tccagattca attctacttc cacgctagtt tccttcgtca
     4141 cccgagacac acttccaatt ctaggtagca cttgttcggt agtgtgagtg ttagcgggct
     4201 ctacgatgac gccaccacct acagaagtgg cactaattct agtttccaac ctcctcgaac
     4261 ttcttccttc cctctgagaa gacgatggta cagcaactga ttttaattcg gagtaaagcg
     4321 gacaataatc gcccacggga taaaactcct cgttcgcttg gcctacaccg acggctgccc
     4381 gagccgcaga actctgttta cgactctgag tcaacaaatc taaggataat cgcccgactg
     4441 ctgctccgat ccagcccagt cctttcgaat tcctcgtact ccgttcaggc gtgccatttc
     4501 cacactgagt tatagctgct ggactcgcca gccgaacagc agaccagtta gcagttgtgg
     4561 ctgccaccca cgccatatct cattgccccc cggatctgcg aaagctcgag agagatagat
     4621 ttgtagagag agactggtga tttcagcgtg tcctctccaa atgaaatgaa cttccttata
     4681 tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga
     4741 tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc
     4801 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag
     4861 cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt
     4921 tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt
     4981 gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt
     5041 agacgagagt gtcgtgctcc accatgttca catcaatcca cttgctttga agacgtggtt
     5101 ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg
     5161 tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg
     5221 ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg
     5281 aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag
     5341 actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat gttggcaagc
     5401 tgctctagcc aatacgcaaa ccgaattcag aaataaattc agcctaattc ggcgttaatt
     5461 cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca
     5521 ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa
     5581 tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta
     5641 aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg
     5701 ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg
     5761 ttgctgcctg tgatcaattc gggcacgaac ccagtggaca taagcctgtt cggttcgtaa
     5821 gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag
     5881 cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca
     5941 gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt
     6001 atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat
     6061 gggggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga
     6121 gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg
     6181 cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac
     6241 aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga
     6301 gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta
     6361 tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat
     6421 cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca
     6481 tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga
     6541 tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg
     6601 cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa
     6661 aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca
     6721 gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc
     6781 gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt
     6841 cggcaaataa tgtctagcta gaaattcgtt caagccgacg ccgcttcgcg gcgcggctta
     6901 actcaagcgt tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc
     6961 tgcttttatt atttttaagc gtgcataata agccctacac aaattgggag atatatcatg
     7021 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7081 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7141 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7201 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     7261 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7321 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7381 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     7441 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     7501 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     7561 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     7621 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     7681 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     7741 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     7801 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     7861 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     7921 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
     7981 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     8041 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     8101 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg
     8161 atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc
     8221 tggccgtagg ccagccattt ttgagcggcc agcggccgcg ataggccgac gcgaagcggc
     8281 ggggcgtagg gagcgcagcg accgaagggt aggcgctttt tgcagctctt cggctgtgcg
     8341 ctggccagac agttatgcac aggccaggcg ggttttaaga gttttaataa gttttaaaga
     8401 gttttaggcg gaaaaatcgc cttttttctc ttttatatca gtcacttaca tgtgtgaccg
     8461 gttcccaatg tacggctttg ggttcccaat gtacgggttc cggttcccaa tgtacggctt
     8521 tgggttccca atgtacgtgc tatccacagg aaagagacct tttcgacctt tttcccctgc
     8581 tagggcaatt tgccctagca tctgctccgt acattaggaa ccggcggatg cttcgccctc
     8641 gatcaggttg cggtagcgca tgactaggat cgggccagcc tgccccgcct cctccttcaa
     8701 atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac agaacttctt
     8761 gaactctccg gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc
     8821 cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa
     8881 aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga tctcgcggta
     8941 catccaatca gctagctcga tctcgatgta ctccggccgc ccggtttcgc tctttacgat
     9001 cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc accaggcggc cgttcttggc
     9061 cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag
     9121 gtcgtctttc tgctttccgc catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg
     9181 tggacggaac acgcggccgg gcttgtctcc cttcccttcc cggtatcggt tcatggattc
     9241 ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg ccatgccggc
     9301 cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg tagcggatca cctcgccagc
     9361 tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc gactatcgcg
     9421 ggtgcccacg tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg
     9481 cttcctaatc gacggcgcac cggctgccgg cggttgccgg gattctttgc ggattcgatc
     9541 agcggccgct tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc
     9601 ggcctgcgcg gccttcaact tctccaccag gtcatcaccc agcgccgcgc cgatttgtac
     9661 cgggccggat ggtttgcgac cgctcacgcc gattcctcgg gcttgggggt tccagtgcca
     9721 ttgcagggcc ggcagacaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac
     9781 atggggcatt ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta
     9841 gccgctaaaa ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat
     9901 gtattcagat agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt
     9961 ggtgtgatcc tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag
    10021 gctggccaac gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt
    10081 tgtgcttttg ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca
    10141 gcggccagcg cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt
    10201 gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg
    10261 ggcagctcgt acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc
    10321 gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta
    10381 accagctcca ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc
    10441 agcacgaagt cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg
    10501 tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg
    10561 tcgatgccga caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg
    10621 ccctggggat cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct
    10681 agatgggttg cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc
    10741 ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg
    10801 catgacgcaa gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt
    10861 cttcagcggc caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga
    10921 tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac ccggccgcga
    10981 tcatctccgc ctcgatctct tcggtaatga aaaacggttc gtcctggccg tcctggtgcg
    11041 gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt
    11101 caatgcgtcc tcacggaagg caccgcgccg cctggcctcg gtgggcgtca cttcctcgct
    11161 gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt
    11221 cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag
    11281 ggtagggcgg gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt
    11341 gcggtcgatg attagggaac gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat
    11401 gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc aggctacgca ggcccgcgcc
    11461 ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc
    11521 tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc
    11581 cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc agccggccgc
    11641 gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc
    11701 cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt
    11761 attctacttt atgcgactaa aacacgcgac aagaaaacgc caggaaaagg gcagggcggc
    11821 agcctgtcgc gtaacttagg acttgtgcga catgtcgttt tcagaagacg gctgcactga
    11881 acgtcagaag ccgactgcac tatagcagcg gaggggttgg atcaaagtac tttgatcccg
    11941 aggggaaccc tgtggttggc atgcacatac aaatggacga acggataaac ct
//
