LOCUS       sequence-393699-       11983 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector for the expression of Citrine with MpIGPDm selective 
            marker in Marchantia polymorpha (igpd mutants) ..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11983)
  AUTHORS   Fukushima T, Kodama Y
  TITLE     Selection of a histidine auxotrophic Marchantia polymorpha strain 
            with an auxotrophic selective marker.
  JOURNAL   Plant Biotechnol (Tokyo). 2022 Dec 25;39(4):345-354. doi: 
            10.5511/plantbiotechnology.22.0810a. Epub 2022 Nov 26.
  PUBMED    37283617
REFERENCE   2  (bases 1 to 11983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.5511/plantbiotechnology.22.0810a"; journalName: "Plant 
            Biotechnol (Tokyo)"; date: "2022-12-25- 25"; volume: "39"; issue: 
            "4"; pages: "345-354"
COMMENT     Sequence Label: pMpGWBhis03-Citrine
FEATURES             Location/Qualifiers
     source          1..11983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2057..2081
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(2084..2086,2087..2089,2090..2800)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2129..2150)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2390..2409)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2737..2758
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(2805..2829)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      2861..3113
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3146..3162)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3146..3162)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3159..3181)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3170..3186
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3194..3200,3201..3218,3219..3224))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3239..3260
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    3345..3519
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(4634..5310)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(4677..4697)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    5505..5529
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6050..6841
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7087..7675
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7576..7595
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7829..7846
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7861..8001
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7927..7946)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8087..8109
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8345..8539
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8605..9678)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10107..10736)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttggcc cgggcaaatg agtcacacac attgttgaga gacatatcaa aataactcaa
      361 ttgtaaggta agttgataga tttcccagtt aaatgcatta ttttaacaat atttgtatct
      421 caaattttca aaatcatatc aaatttgcag atcttgatga tgattcacaa ctctgtcttc
      481 gatttttgat tgttaacggg tatcgatatt tgggtaactt aaaaagaatt tgctaaagat
      541 cttaaaaaac agattaaaaa aacctttcaa aatagctaat cctttaaatc cgtttcacaa
      601 tataatgctc attgatgata atcgaaaaga ttccatttta aagtgcatat aaattgctta
      661 attatctctt tctgtctcgt aatattttgt tctctcctat tggatcggac ccgcatcttc
      721 tcgatcagtt cactatacta atatttggtc aacttcaaga gagattagaa cttcgttctc
      781 caaagtgtaa aggccgacaa gcagaaaagg aaacttcaat cagaagcctt ctaggttttc
      841 ccgcgtccca aaaagcgaag ggaggacggg gaggacggtg aggccagaag aagaaagcaa
      901 cagtaaaatc tcatgtgatt gcgtgcatgt catggatcta tgggatgcta gtccaccgtt
      961 tggaagtatg tgcagaagga gaaaggcgcg ggaggccaaa agcgacttcg gccacctctc
     1021 tactttctag aaagtttgct cgcagcttgt aataagcgcg gaaaaggtac tcgaaaaacc
     1081 ggagcaatcg gtgtggtatc agcagtcggg ttcgctaaac ctacgtcttc ttattttgga
     1141 atgttaaatt gaaagggcgt gccggcccta agcacggacg atgtgacaga cggaggagtg
     1201 tgagcaattc ggatgacgat gctggccggc agtcacgaca aagagtccga aggggcatgg
     1261 acagggcaat atataaatgg aaaagaggct ttgaatattc ctaacccttc gtcgtcggcc
     1321 tcccaacttg ccctcgcatg ctgaattggc cgcctatctg cgaatggccg gccgccgacc
     1381 ctaagtcgca ataacttgaa cggagccgat gcaaagtcct tatcgaacgc tcgttattat
     1441 aaacggggca gacctaggta tttaattata tcaaaaccct ccgcaatttc gctaatcatt
     1501 ccctaatttc ttctactttg gcgtctagtg ctgtggtcgc gcttgctctc aaagctcgct
     1561 gggaggcagt ggagcgtctg gcttaaaatc gatcgccgca accctagagt tcctttcact
     1621 tctttctctc ttgcgtggag agcatcgaca tcgaagagct gcatcttgtc ttctggtatg
     1681 cgccttgtgc cctcgtctct gtacgtcgtt acgattgctc gatattgtga ttgtctagac
     1741 ctgaaacccg actggagctt ttacgtggtg tcgattgggg cctcgagcga gtgatttttt
     1801 aggttcgttt tagccaagta catcccactt tggtcagtcc tgttatctcg gtaaggtgtt
     1861 cagttcggtc tctggtgcgg aggtgcctcg taagtttcct gcatgataga tttgtctatc
     1921 cttgacttga agaggagtcg aaggtcagcg aggttggctg tgtgctatgg acgtcggtgc
     1981 tccttggttc cgtgcttcta tgggtgttgc ctcaacagta ttgatgtgcc tgcagaaagg
     2041 ttgactagag ttatcaacaa gtttgtacaa aaaagcaggc tccatggtga gcaagggcga
     2101 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     2161 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     2221 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg
     2281 ctacggcctg atgtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
     2341 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     2401 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     2461 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     2521 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     2581 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     2641 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc
     2701 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     2761 cgccgccggg atcactctcg gcatggacga gctgtacaag tgagacccag ctttcttgta
     2821 caaagtggtt gataacagcg cttagagctc gaatttcccc gatcgttcaa acatttggca
     2881 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct
     2941 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
     3001 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata
     3061 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt
     3121 ggttccggaa ccaattcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc
     3181 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     3241 gagtgagcta actcacatta ggctgaatta ggcgcgccta tttctgaatt caacacattg
     3301 cggacgtttt taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta
     3361 ctggattttg gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat
     3421 acatactaag ggtttcttat atgctcaaca catgagcgaa accctatagg aaccctaatt
     3481 cccttatctg ggaactactc acacattatt atggagaaac tcgagcttgt cgatcgacag
     3541 atcccggtcg gcatctactt catgcgcgag ataagacccc cttagagctt ggaacggatc
     3601 cctttcttct cgtatcatat tcaagcgcct gtcgcagagc tctggcaaaa gctttgaaag
     3661 tagcctcgat gatgtggtga gaattggttc ccgcaagctt gcgaatatgg agggtcattc
     3721 cagacgtgtt caccaccgat tggaaaaaat gctccaccaa ctgtgtatcg taggtaccaa
     3781 ctcgttgagt gggaatgtct acattatacc ccaaaaatgg tcttcctgat aaatccaaaa
     3841 caacatggat cagagcttca tcaagtggag ccgaaaaatc cccaaagcga tatatgccct
     3901 tcctatctcc taaggcctgg agtaatgcag ttccgacagc caaaccaatg tcctcgttcg
     3961 tgtgatgatc atcaatgtga atgtcgccct ttgccttcac tgtcacatct aacagtccat
     4021 gcgacgcaag ttgatctagc atgtgatcga gaaagggtat gccggtgtca cagtcgcaga
     4081 ttcctgtccc gtccagattc aattctactt ccacgctagt ttccttcgtc acccgagaca
     4141 cacttccaat tctaggtagc acttgttcgg tagtgtgagt gttagcgggc tctacgatga
     4201 cgccaccacc tacagaagtg gcactaattc tagtttccaa cctcctcgaa cttcttcctt
     4261 ccctctgaga agacgatggt acagcaactg attttaattc ggagtaaagc ggacaataat
     4321 cgcccacggg ataaaactcc tcgttcgctt ggcctacacc gacggctgcc cgagccgcag
     4381 aactctgttt acgactctga gtcaacaaat ctaaggataa tcgcccgact gctgctccga
     4441 tccagcccag tcctttcgaa ttcctcgtac tccgttcagg cgtgccattt ccacactgag
     4501 ttatagctgc tggactcgcc agccgaacag cagaccagtt agcagttgtg gctgccaccc
     4561 acgccatatc tcattgcccc ccggatctgc gaaagctcga gagagataga tttgtagaga
     4621 gagactggtg atttcagcgt gtcctctcca aatgaaatga acttccttat atagaggaag
     4681 ggtcttgcga aggatagtgg gattgtgcgt catcccttac gtcagtggag atatcacatc
     4741 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg
     4801 gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt
     4861 tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt
     4921 gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag
     4981 tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag tagacgagag
     5041 tgtcgtgctc caccatgttc acatcaatcc acttgctttg aagacgtggt tggaacgtct
     5101 tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag
     5161 gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc
     5221 ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt
     5281 cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct
     5341 ttgatattct tggagtagac gagagtgtcg tgctccacca tgttggcaag ctgctctagc
     5401 caatacgcaa accgaattca gaaataaatt cagcctaatt cggcgttaat tcagtacatt
     5461 aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat
     5521 atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc
     5581 gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag
     5641 actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa
     5701 cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct
     5761 gtgatcaatt cgggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg
     5821 caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa
     5881 cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc
     5941 tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag
     6001 caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgggggaagc
     6061 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     6121 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     6181 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     6241 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     6301 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     6361 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     6421 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     6481 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     6541 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     6601 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     6661 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     6721 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     6781 tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     6841 atgtctagct agaaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg
     6901 ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat
     6961 tatttttaag cgtgcataat aagccctaca caaattggga gatatatcat gcatgaccaa
     7021 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     7081 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     7141 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     7201 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     7261 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     7321 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     7381 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     7441 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     7501 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     7561 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     7621 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     7681 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     7741 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     7801 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     7861 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     7921 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     7981 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     8041 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     8101 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg
     8161 ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc ctggccgtag
     8221 gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg cggggcgtag
     8281 ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc gctggccaga
     8341 cagttatgca caggccaggc gggttttaag agttttaata agttttaaag agttttaggc
     8401 ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc ggttcccaat
     8461 gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct ttgggttccc
     8521 aatgtacgtg ctatccacag gaaagagacc ttttcgacct ttttcccctg ctagggcaat
     8581 ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct cgatcaggtt
     8641 gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca aatcgtactc
     8701 cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct tgaactctcc
     8761 ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg ccttgcctgc
     8821 ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca aaaagtaatc
     8881 ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt acatccaatc
     8941 agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga tcttgtagcg
     9001 gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg ccttcttcgt
     9061 acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt
     9121 ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt gtggacggaa
     9181 cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt cggttagatg
     9241 ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg ccggccctgc
     9301 ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag ctcgtcggtc
     9361 acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc gggtgcccac
     9421 gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat
     9481 cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat cagcggccgc
     9541 ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc
     9601 ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga
     9661 tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc attgcagggc
     9721 cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca catggggcat
     9781 tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt agccgctaaa
     9841 attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga
     9901 tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc
     9961 ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa
    10021 cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt
    10081 gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc
    10141 gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg
    10201 gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg
    10261 tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac
    10321 acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc
    10381 accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag
    10441 tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact
    10501 acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg
    10561 acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga
    10621 tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt
    10681 gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt
    10741 tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca
    10801 agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg
    10861 ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca
    10921 gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg atcatctccg
    10981 cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc
    11041 ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc
    11101 ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag
    11161 tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg
    11221 gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg
    11281 ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat
    11341 gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc
    11401 cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc cggcctcctg
    11461 gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt
    11521 cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc
    11581 gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg cgtgcagttc
    11641 ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc
    11701 agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag tattctactt
    11761 tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg
    11821 cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa
    11881 gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc gaggggaacc
    11941 ctgtggttgg catgcacata caaatggacg aacggataaa cct
//
