LOCUS       sequence-393700-       13477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Binary vector with MpIGPDm as a selective marker in Marchantia 
            polymorpha (igpd mutants) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13477)
  AUTHORS   Fukushima T, Kodama Y
  TITLE     Selection of a histidine auxotrophic Marchantia polymorpha strain 
            with an auxotrophic selective marker.
  JOURNAL   Plant Biotechnol (Tokyo). 2022 Dec 25;39(4):345-354. doi: 
            10.5511/plantbiotechnology.22.0810a. Epub 2022 Nov 26.
  PUBMED    37283617
REFERENCE   2  (bases 1 to 13477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.5511/plantbiotechnology.22.0810a"; journalName: "Plant 
            Biotechnol (Tokyo)"; date: "2022-12-25- 25"; volume: "39"; issue: 
            "4"; pages: "345-354"
COMMENT     Sequence Label: pMpGWBhis03
FEATURES             Location/Qualifiers
     source          1..13477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2057..2181
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2610..2915)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(2712..2731)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(3235..3918)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     3833..3852
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3919..4021)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(4199..4323)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      4355..4607
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4640..4656)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4640..4656)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4653..4675)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4664..4680
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4688..4694,4695..4712,4713..4718))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4733..4754
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4839..5013
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(6128..6804)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(6171..6191)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    6999..7023
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7544..8335
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8581..9169
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9070..9089
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9323..9340
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9355..9495
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9421..9440)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9581..9603
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9839..10033
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10099..11172)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11601..12230)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttggcc cgggcaaatg agtcacacac attgttgaga gacatatcaa aataactcaa
      361 ttgtaaggta agttgataga tttcccagtt aaatgcatta ttttaacaat atttgtatct
      421 caaattttca aaatcatatc aaatttgcag atcttgatga tgattcacaa ctctgtcttc
      481 gatttttgat tgttaacggg tatcgatatt tgggtaactt aaaaagaatt tgctaaagat
      541 cttaaaaaac agattaaaaa aacctttcaa aatagctaat cctttaaatc cgtttcacaa
      601 tataatgctc attgatgata atcgaaaaga ttccatttta aagtgcatat aaattgctta
      661 attatctctt tctgtctcgt aatattttgt tctctcctat tggatcggac ccgcatcttc
      721 tcgatcagtt cactatacta atatttggtc aacttcaaga gagattagaa cttcgttctc
      781 caaagtgtaa aggccgacaa gcagaaaagg aaacttcaat cagaagcctt ctaggttttc
      841 ccgcgtccca aaaagcgaag ggaggacggg gaggacggtg aggccagaag aagaaagcaa
      901 cagtaaaatc tcatgtgatt gcgtgcatgt catggatcta tgggatgcta gtccaccgtt
      961 tggaagtatg tgcagaagga gaaaggcgcg ggaggccaaa agcgacttcg gccacctctc
     1021 tactttctag aaagtttgct cgcagcttgt aataagcgcg gaaaaggtac tcgaaaaacc
     1081 ggagcaatcg gtgtggtatc agcagtcggg ttcgctaaac ctacgtcttc ttattttgga
     1141 atgttaaatt gaaagggcgt gccggcccta agcacggacg atgtgacaga cggaggagtg
     1201 tgagcaattc ggatgacgat gctggccggc agtcacgaca aagagtccga aggggcatgg
     1261 acagggcaat atataaatgg aaaagaggct ttgaatattc ctaacccttc gtcgtcggcc
     1321 tcccaacttg ccctcgcatg ctgaattggc cgcctatctg cgaatggccg gccgccgacc
     1381 ctaagtcgca ataacttgaa cggagccgat gcaaagtcct tatcgaacgc tcgttattat
     1441 aaacggggca gacctaggta tttaattata tcaaaaccct ccgcaatttc gctaatcatt
     1501 ccctaatttc ttctactttg gcgtctagtg ctgtggtcgc gcttgctctc aaagctcgct
     1561 gggaggcagt ggagcgtctg gcttaaaatc gatcgccgca accctagagt tcctttcact
     1621 tctttctctc ttgcgtggag agcatcgaca tcgaagagct gcatcttgtc ttctggtatg
     1681 cgccttgtgc cctcgtctct gtacgtcgtt acgattgctc gatattgtga ttgtctagac
     1741 ctgaaacccg actggagctt ttacgtggtg tcgattgggg cctcgagcga gtgatttttt
     1801 aggttcgttt tagccaagta catcccactt tggtcagtcc tgttatctcg gtaaggtgtt
     1861 cagttcggtc tctggtgcgg aggtgcctcg taagtttcct gcatgataga tttgtctatc
     1921 cttgacttga agaggagtcg aaggtcagcg aggttggctg tgtgctatgg acgtcggtgc
     1981 tccttggttc cgtgcttcta tgggtgttgc ctcaacagta ttgatgtgcc tgcagaaagg
     2041 ttgactagag ttatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat
     2101 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
     2161 acaacatatc cagtcactat gaatcaacta cttagatggt attagtgacc tgtagtcgac
     2221 cgacagcctt ccaaatgttc ttcgggtgat gctgccaact tagtcgaccg acagccttcc
     2281 aaatgttctt ctcaaacgga atcgtcgtat ccagcctact cgctattgtc ctcaatgccg
     2341 tattaaatca taaaaagaaa taagaaaaag aggtgcgagc ctcttttttg tgtgacaaaa
     2401 taaaaacatc tacctattca tatacgctag tgtcatagtc ctgaaaatca tctgcatcaa
     2461 gaacaatttc acaactctta tacttttctc ttacaagtcg ttcggcttca tctggatttt
     2521 cagcctctat acttactaaa cgtgataaag tttctgtaat ttctactgta tcgacctgca
     2581 gactggctgt gtataaggga gcctgacatt tatattcccc agaacatcag gttaatggcg
     2641 tttttgatgt cattttcgcg gtggctgaga tcagccactt cttccccgat aacggagacc
     2701 ggcacactgg ccatatcggt ggtcatcatg cgccagcttt catccccgat atgcaccacc
     2761 gggtaaagtt cacgggagac tttatctgac agcagacgtg cactggccag ggggatcacc
     2821 atccgtcgcc cgggcgtgtc aataatatca ctctgtacat ccacaaacag acgataacgg
     2881 ctctctcttt tataggtgta aaccttaaac tgcatttcac cagcccctgt tctcgtcagc
     2941 aaaagagccg ttcatttcaa taaaccgggc gacctcagcc atcccttcct gattttccgc
     3001 tttccagcgt tcggcacgca gacgacgggc ttcattctgc atggttgtgc ttaccagacc
     3061 ggagatattg acatcatata tgccttgagc aactgatagc tgtcgctgtc aactgtcact
     3121 gtaatacgct gcttcatagc atacctcttt ttgacatact tcgggtatac atatcagtat
     3181 atattcttat accgcaaaaa tcagcgcgca aatacgcata ctgttatctg gcttttagta
     3241 agccggatcc acgcggcgtt tacgcccccc ctgccactca tcgcagtact gttgtaattc
     3301 attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag
     3361 cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa
     3421 gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc
     3481 tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta
     3541 acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact
     3601 ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact
     3661 atcccatatc accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat
     3721 caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt
     3781 ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga
     3841 ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc
     3901 agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa
     3961 tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc
     4021 aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg
     4081 atttatttat tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgtc
     4141 gggtgatgct gccaacttag tcgactacag gtcactaata ccatctaagt agttgattca
     4201 tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta
     4261 atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt
     4321 ggttgataac agcgcttaga gctcgaattt ccccgatcgt tcaaacattt ggcaataaag
     4381 tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa
     4441 ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt
     4501 tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc
     4561 aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg aattggttcc
     4621 ggaaccaatt cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     4681 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     4741 gctaactcac attaggctga attaggcgcg cctatttctg aattcaacac attgcggacg
     4801 tttttaatgt actgaattaa cgccgaatta attcggggga tctggatttt agtactggat
     4861 tttggtttta ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac
     4921 taagggtttc ttatatgctc aacacatgag cgaaacccta taggaaccct aattccctta
     4981 tctgggaact actcacacat tattatggag aaactcgagc ttgtcgatcg acagatcccg
     5041 gtcggcatct acttcatgcg cgagataaga cccccttaga gcttggaacg gatccctttc
     5101 ttctcgtatc atattcaagc gcctgtcgca gagctctggc aaaagctttg aaagtagcct
     5161 cgatgatgtg gtgagaattg gttcccgcaa gcttgcgaat atggagggtc attccagacg
     5221 tgttcaccac cgattggaaa aaatgctcca ccaactgtgt atcgtaggta ccaactcgtt
     5281 gagtgggaat gtctacatta taccccaaaa atggtcttcc tgataaatcc aaaacaacat
     5341 ggatcagagc ttcatcaagt ggagccgaaa aatccccaaa gcgatatatg cccttcctat
     5401 ctcctaaggc ctggagtaat gcagttccga cagccaaacc aatgtcctcg ttcgtgtgat
     5461 gatcatcaat gtgaatgtcg ccctttgcct tcactgtcac atctaacagt ccatgcgacg
     5521 caagttgatc tagcatgtga tcgagaaagg gtatgccggt gtcacagtcg cagattcctg
     5581 tcccgtccag attcaattct acttccacgc tagtttcctt cgtcacccga gacacacttc
     5641 caattctagg tagcacttgt tcggtagtgt gagtgttagc gggctctacg atgacgccac
     5701 cacctacaga agtggcacta attctagttt ccaacctcct cgaacttctt ccttccctct
     5761 gagaagacga tggtacagca actgatttta attcggagta aagcggacaa taatcgccca
     5821 cgggataaaa ctcctcgttc gcttggccta caccgacggc tgcccgagcc gcagaactct
     5881 gtttacgact ctgagtcaac aaatctaagg ataatcgccc gactgctgct ccgatccagc
     5941 ccagtccttt cgaattcctc gtactccgtt caggcgtgcc atttccacac tgagttatag
     6001 ctgctggact cgccagccga acagcagacc agttagcagt tgtggctgcc acccacgcca
     6061 tatctcattg ccccccggat ctgcgaaagc tcgagagaga tagatttgta gagagagact
     6121 ggtgatttca gcgtgtcctc tccaaatgaa atgaacttcc ttatatagag gaagggtctt
     6181 gcgaaggata gtgggattgt gcgtcatccc ttacgtcagt ggagatatca catcaatcca
     6241 cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg
     6301 gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc
     6361 aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga
     6421 tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa
     6481 tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt
     6541 gctccaccat gttcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt
     6601 tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct
     6661 tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct
     6721 actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat
     6781 attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata
     6841 ttcttggagt agacgagagt gtcgtgctcc accatgttgg caagctgctc tagccaatac
     6901 gcaaaccgaa ttcagaaata aattcagcct aattcggcgt taattcagta cattaaaaac
     6961 gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg
     7021 ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca
     7081 ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg
     7141 ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga
     7201 tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc
     7261 aattcgggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta
     7321 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
     7381 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
     7441 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     7501 tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatggggg aagcggtgat
     7561 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
     7621 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
     7681 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
     7741 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
     7801 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
     7861 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
     7921 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
     7981 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
     8041 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
     8101 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
     8161 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
     8221 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
     8281 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct
     8341 agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttagat
     8401 gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt
     8461 taagcgtgca taataagccc tacacaaatt gggagatata tcatgcatga ccaaaatccc
     8521 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     8581 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     8641 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     8701 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     8761 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     8821 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     8881 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     8941 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     9001 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     9061 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     9121 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     9181 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     9241 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     9301 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
     9361 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
     9421 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
     9481 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     9541 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     9601 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
     9661 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     9721 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     9781 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     9841 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     9901 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     9961 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
    10021 cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc
    10081 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
    10141 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
    10201 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
    10261 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
    10321 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
    10381 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
    10441 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
    10501 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
    10561 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
    10621 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
    10681 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
    10741 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
    10801 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
    10861 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
    10921 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
    10981 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
    11041 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
    11101 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
    11161 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
    11221 acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
    11281 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
    11341 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
    11401 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
    11461 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
    11521 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
    11581 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
    11641 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
    11701 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
    11761 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
    11821 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
    11881 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
    11941 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
    12001 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
    12061 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
    12121 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
    12181 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
    12241 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
    12301 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
    12361 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
    12421 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
    12481 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
    12541 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
    12601 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
    12661 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
    12721 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
    12781 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
    12841 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
    12901 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
    12961 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
    13021 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
    13081 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
    13141 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
    13201 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
    13261 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
    13321 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
    13381 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
    13441 ttggcatgca catacaaatg gacgaacgga taaacct
//
