LOCUS       sequence-39381-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Maeder ML, Thibodeau-Beganny S, Sander JD, Voytas DF, Joung JK
  TITLE     Oligomerized pool engineering (OPEN): an 'open-source' protocol for 
            making customized zinc-finger arrays.
  JOURNAL   Nat Protoc. 2009. 4(10):1471-501.
  PUBMED    19798082
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2009. 4(10):1471-501."
COMMENT     Sequence Label: pMLM36 (aka pBR-UV5-GP-FD2)
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(240..263)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(554..576)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    565..581
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(589..595,596..613,614..619))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             746..937
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc
       61 gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
      121 gctattacgc caggatcttc agcggagcgg gacttgtcgg cctttttcta gtctctagtt
      181 actactgtgc atgtcttctt acttaggatc ctcacttaga agacctcccc gggtctagat
      241 ttgtcatcat cgtctttgta gtcagcggcc gccaaagctt ggatttttct cagggccttt
      301 tgcacaatgt atttcctaat aaaactttgc ttgacaggtt ccgtcatcgt actacccaat
      361 tgtgtttttt caaaattctt gcaggtttcg atggcttctt gtaataagct gcgttgctcc
      421 tctggagaaa agatttggtt aatttggttg aggacgtttg gtacggtatt ttgtgatgat
      481 tgctgttgca aagggttgtt gtccgcactt gaattgggag gttgcatttg ctgctgttga
      541 ggcatagctg tttcctgtgt gaaattgtta tccgctcaca agtcgacaca ttatacgagc
      601 cggaagcata aagtgtaaag cccggaattc ccgttccagt aaccgggcat gttcatcatc
      661 agtaacccgt atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag
      721 aaatccccct tacacggagg catcagtgac caaacaggaa aaaaccgccc ttaacatggc
      781 ccgctttatc agaagccaga cattaacgct tctggagaaa ctcaacgagc tggacgcgga
      841 tgaacaggca gacatctgtg aatcgcttca cgaccacgct gatgagcttt accgcagctg
      901 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc c
//
