LOCUS       sequence-39385-e         301 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Nemoto S, Fergusson MM, Finkel T
  TITLE     SIRT1 functionally interacts with the metabolic regulator and 
            transcriptional coactivator PGC-1{alpha}.
  JOURNAL   J Biol Chem. 2005 Apr 22. 280(16):16456-60.
  PUBMED    15716268
REFERENCE   2  (bases 1 to 301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2005 Apr 22. 280(16):16456-60."
COMMENT     Sequence Label: pCruzHA SIRT1
FEATURES             Location/Qualifiers
     source          1..301
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..60
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        41..59
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      82..91
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             97..123
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             103..129
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     regulatory      274..283
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tggctactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
       61 agacccaagc ttctcgaggc cgccaccatg ggatcctacc cttacgacgt tcctgattac
      121 gctagcctcg aattcagtac tgcggccgcg gtaccgagct cggatccact agtccagtgt
      181 ggtggaattc tgcggattcg acagagaggc agttggaaga tggcggacga ggtggcgctc
      241 gcccttcagg ccgccggctc cccttccgcg gcggccgcca tggaggccgc gtcgcagccg
      301 g
//
