LOCUS       sequence-39386-e         533 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Nemoto S, Fergusson MM, Finkel T
  TITLE     SIRT1 functionally interacts with the metabolic regulator and 
            transcriptional coactivator PGC-1{alpha}.
  JOURNAL   J Biol Chem. 2005 Apr 22. 280(16):16456-60.
  PUBMED    15716268
REFERENCE   2  (bases 1 to 533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2005 Apr 22. 280(16):16456-60."
COMMENT     Sequence Label: pCruzHA SIRT1 G261A
FEATURES             Location/Qualifiers
     source          1..533
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      69..78
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             84..110
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             90..116
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     regulatory      261..270
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagcttc
       61 tcgaggccgc caccatggga tcctaccctt acgacgttcc tgattacgct agcctcgaat
      121 tcagtactgc ggccgcggta ccgagctcgg atccactagt ccagtgtggt ggaattctgc
      181 agattcgaca gagaggcagt tggaagatgg cggacgaggt ggcgctcgcc cttcaggccg
      241 ccggctcccc ttccgcggcg gccgccatgg aggccgcgtc gcagccggcg gacgagccgc
      301 tccgcaagag gccccgccga gacgggcctg gcctcgggcg cagcccgggc gagccgagcg
      361 cagcagtggc gccggcggcc gcggggtgtg aggcggcgag cgccgcggcc ccggcggcgc
      421 tgtggcggga ggcggcaggg gcggcggcga gcgcggagcg ggaggccccg gcgacggccg
      481 tggccgggga cggagacaat gggtccggcc tgcggcggga gccgagggcg gcc
//
